Publicação
Biofunctionalization of Bacterial Cellulose with FucoPol Enhances Keratinocyte Activity and Wound Closure
| Resumo: | Bacterial cellulose (BC), a renewable microbially produced biocompatible polymer, is widely studied as a wound dressing given its nanofibrillar structure, mechanical strength, and high water retention capacity. However, pristine BC is largely biologically inert and does not actively stimulate tissue regeneration, highlighting the need for its biofunctionalization. Effective wound care requires dressings that both protect the wound environment and actively promote healing, driving growing interest in advanced and affordable bioactive wound dressings. FucoPol (FP), a fucose-containing, polyanionic microbial polysaccharide with proven wound-healing, antioxidant, and photoprotective properties, offers an attractive strategy to impart bioactivity to BC. In this study, BC membranes were functionalized by impregnation with an FP solution, enabling efficient diffusion and loading of the bioactive polysaccharide. The resulting FP-functionalized membranes presented reduced crystallinity (CI = 47 vs 56%) and water-holding capacity (41 ± 2.1 vs 75 ± 0.9 g/g). However, they outperformed neat BC in biological properties; BC/FP membranes promoted keratinocyte proliferation and metabolic activity and accelerated wound closure (28.3 ± 7.5% at 24 h). Overall, FP-functionalized BC membranes emerge as promising materials for developing bioactive wound dressings with clear enhanced therapeutic potential, particularly for early-stage wound management. |
|---|---|
| Autores principais: | Esmail, Asiyah |
| Outros Autores: | Rovisco, Ana; Morais, Maria; Fortunato, Elvira; Serra, Ana Teresa; Freitas, Filomena |
| Assunto: | Bacterial cellulose Composite Hydrogel Polysaccharide Wound healing Biomaterials General Chemistry Biomedical Engineering Biochemistry, medical SDG 7 - Affordable and Clean Energy |
| Ano: | 2026 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| _version_ | 1879446021997592576 |
|---|---|
| author | Esmail, Asiyah |
| author2 | Rovisco, Ana Morais, Maria Fortunato, Elvira Serra, Ana Teresa Freitas, Filomena |
| author2_role | author author author author author |
| author_facet | Esmail, Asiyah Rovisco, Ana Morais, Maria Fortunato, Elvira Serra, Ana Teresa Freitas, Filomena |
| author_role | author |
| contributor_name_str_mv | DQ - Departamento de Química UCIBIO - Applied Molecular Biosciences Unit UNINOVA-Instituto de Desenvolvimento de Novas Tecnologias CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N) DCM - Departamento de Ciência dos Materiais Instituto de Tecnologia Química e Biológica António Xavier (ITQB) Faculdade de Ciências e Tecnologia (FCT) ACS - American Chemical Society RUN |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Esmail, Asiyah\"},{\"Person.name\":\"Rovisco, Ana\"},{\"Person.name\":\"Morais, Maria\"},{\"Person.name\":\"Fortunato, Elvira\"},{\"Person.name\":\"Serra, Ana Teresa\"},{\"Person.name\":\"Freitas, Filomena\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | DQ - Departamento de Química UCIBIO - Applied Molecular Biosciences Unit UNINOVA-Instituto de Desenvolvimento de Novas Tecnologias CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N) DCM - Departamento de Ciência dos Materiais Instituto de Tecnologia Química e Biológica António Xavier (ITQB) Faculdade de Ciências e Tecnologia (FCT) ACS - American Chemical Society RUN |
| datacite.creators.creator.creatorName.fl_str_mv | Esmail, Asiyah Rovisco, Ana Morais, Maria Fortunato, Elvira Serra, Ana Teresa Freitas, Filomena |
| datacite.date.Accepted.fl_str_mv | 2026-06-30T00:00:00Z |
| datacite.date.available.fl_str_mv | 2026-09-11T15:00:01Z |
| datacite.date.embargoed.fl_str_mv | 2026-09-11T15:00:01Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Bacterial cellulose Composite Hydrogel Polysaccharide Wound healing Biomaterials General Chemistry Biomedical Engineering Biochemistry, medical SDG 7 - Affordable and Clean Energy |
| datacite.titles.title.fl_str_mv | Biofunctionalization of Bacterial Cellulose with FucoPol Enhances Keratinocyte Activity and Wound Closure |
| dc.contributor.none.fl_str_mv | DQ - Departamento de Química UCIBIO - Applied Molecular Biosciences Unit UNINOVA-Instituto de Desenvolvimento de Novas Tecnologias CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N) DCM - Departamento de Ciência dos Materiais Instituto de Tecnologia Química e Biológica António Xavier (ITQB) Faculdade de Ciências e Tecnologia (FCT) ACS - American Chemical Society RUN |
| dc.creator.none.fl_str_mv | Esmail, Asiyah Rovisco, Ana Morais, Maria Fortunato, Elvira Serra, Ana Teresa Freitas, Filomena |
| dc.date.Accepted.fl_str_mv | 2026-06-30T00:00:00Z |
| dc.date.available.fl_str_mv | 2026-09-11T15:00:01Z |
| dc.date.embargoed.fl_str_mv | 2026-09-11T15:00:01Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10362/206327 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Bacterial cellulose Composite Hydrogel Polysaccharide Wound healing Biomaterials General Chemistry Biomedical Engineering Biochemistry, medical SDG 7 - Affordable and Clean Energy |
| dc.title.fl_str_mv | Biofunctionalization of Bacterial Cellulose with FucoPol Enhances Keratinocyte Activity and Wound Closure |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Bacterial cellulose (BC), a renewable microbially produced biocompatible polymer, is widely studied as a wound dressing given its nanofibrillar structure, mechanical strength, and high water retention capacity. However, pristine BC is largely biologically inert and does not actively stimulate tissue regeneration, highlighting the need for its biofunctionalization. Effective wound care requires dressings that both protect the wound environment and actively promote healing, driving growing interest in advanced and affordable bioactive wound dressings. FucoPol (FP), a fucose-containing, polyanionic microbial polysaccharide with proven wound-healing, antioxidant, and photoprotective properties, offers an attractive strategy to impart bioactivity to BC. In this study, BC membranes were functionalized by impregnation with an FP solution, enabling efficient diffusion and loading of the bioactive polysaccharide. The resulting FP-functionalized membranes presented reduced crystallinity (CI = 47 vs 56%) and water-holding capacity (41 ± 2.1 vs 75 ± 0.9 g/g). However, they outperformed neat BC in biological properties; BC/FP membranes promoted keratinocyte proliferation and metabolic activity and accelerated wound closure (28.3 ± 7.5% at 24 h). Overall, FP-functionalized BC membranes emerge as promising materials for developing bioactive wound dressings with clear enhanced therapeutic potential, particularly for early-stage wound management. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://run.unl.pt/bitstreams/10724292-1d99-41ba-b24b-912ecc263bc6/download |
| id | run_3db2b66af0d5f465ae25fd3950ebabfa |
| identifier.url.fl_str_mv | http://hdl.handle.net/10362/206327 |
| inst_facet_str | urn:organizationAcronym:unl{{{_:::_}}}Universidade Nova de Lisboa |
| instacron_str | unl |
| institution | Universidade Nova de Lisboa |
| instname_str | Universidade Nova de Lisboa |
| language | eng |
| network_acronym_str | run |
| network_name_str | Repositório Institucional da UNL |
| oai_identifier_str | oai:run.unl.pt:10362/206327 |
| organization_str_mv | urn:organizationAcronym:unl |
| person_str_mv | Esmail, Asiyah Rovisco, Ana Morais, Maria Fortunato, Elvira Serra, Ana Teresa Freitas, Filomena |
| publishDate | 2026 |
| repo_facet_str | urn:repositoryAcronym:run{{{_:::_}}}Repositório Institucional da UNL |
| reponame_str | Repositório Institucional da UNL |
| repository_id_str | urn:repositoryAcronym:run |
| service_str_mv | urn:repositoryAcronym:run |
| spelling | engenBacterial cellulose (BC), a renewable microbially produced biocompatible polymer, is widely studied as a wound dressing given its nanofibrillar structure, mechanical strength, and high water retention capacity. However, pristine BC is largely biologically inert and does not actively stimulate tissue regeneration, highlighting the need for its biofunctionalization. Effective wound care requires dressings that both protect the wound environment and actively promote healing, driving growing interest in advanced and affordable bioactive wound dressings. FucoPol (FP), a fucose-containing, polyanionic microbial polysaccharide with proven wound-healing, antioxidant, and photoprotective properties, offers an attractive strategy to impart bioactivity to BC. In this study, BC membranes were functionalized by impregnation with an FP solution, enabling efficient diffusion and loading of the bioactive polysaccharide. The resulting FP-functionalized membranes presented reduced crystallinity (CI = 47 vs 56%) and water-holding capacity (41 ± 2.1 vs 75 ± 0.9 g/g). However, they outperformed neat BC in biological properties; BC/FP membranes promoted keratinocyte proliferation and metabolic activity and accelerated wound closure (28.3 ± 7.5% at 24 h). Overall, FP-functionalized BC membranes emerge as promising materials for developing bioactive wound dressings with clear enhanced therapeutic potential, particularly for early-stage wound management.application/pdfenBiofunctionalization of Bacterial Cellulose with FucoPol Enhances Keratinocyte Activity and Wound ClosureEsmail, AsiyahRovisco, AnaMorais, MariaFortunato, ElviraSerra, Ana TeresaFreitas, FilomenaDQ - Departamento de QuímicaUCIBIO - Applied Molecular Biosciences UnitUNINOVA-Instituto de Desenvolvimento de Novas TecnologiasCENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)DCM - Departamento de Ciência dos MateriaisInstituto de Tecnologia Química e Biológica António Xavier (ITQB)Faculdade de Ciências e Tecnologia (FCT)ACS - American Chemical SocietyHostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptISSNIsPartOf2576-6422URNIsPartOfPURE: 172749504URNIsPartOfPURE UUID: fc467b1a-0804-4dc7-a8f4-c75f629618f6URNIsPartOfScopus: 105045025461URNIsPartOfPubMed: 42377006URNIsPartOfWOS: 001808039000001URNIsPartOfORCID: /0000-0002-4202-7047/work/226484120URNIsPartOfORCID: /0000-0002-9430-4640/work/226484739URNIsPartOfORCID: /0000-0001-6240-3743/work/226484857DOIIsPartOf10.1021/acsabm.6c006492026-09-11T15:00:01Z2026-06-302026-06-30T00:00:00ZHandlehttp://hdl.handle.net/10362/206327http://purl.org/coar/access_right/c_abf2open accessBacterial celluloseCompositeHydrogelPolysaccharideWound healingBiomaterialsGeneral ChemistryBiomedical EngineeringBiochemistry, medicalSDG 7 - Affordable and Clean Energy3590365 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/10724292-1d99-41ba-b24b-912ecc263bc6/download |
| spellingShingle | Biofunctionalization of Bacterial Cellulose with FucoPol Enhances Keratinocyte Activity and Wound Closure Esmail, Asiyah Bacterial cellulose Composite Hydrogel Polysaccharide Wound healing Biomaterials General Chemistry Biomedical Engineering Biochemistry, medical SDG 7 - Affordable and Clean Energy |
| status | SINGLETON |
| subject.fl_str_mv | Bacterial cellulose Composite Hydrogel Polysaccharide Wound healing Biomaterials General Chemistry Biomedical Engineering Biochemistry, medical SDG 7 - Affordable and Clean Energy |
| title | Biofunctionalization of Bacterial Cellulose with FucoPol Enhances Keratinocyte Activity and Wound Closure |
| title_full | Biofunctionalization of Bacterial Cellulose with FucoPol Enhances Keratinocyte Activity and Wound Closure |
| title_fullStr | Biofunctionalization of Bacterial Cellulose with FucoPol Enhances Keratinocyte Activity and Wound Closure |
| title_full_unstemmed | Biofunctionalization of Bacterial Cellulose with FucoPol Enhances Keratinocyte Activity and Wound Closure |
| title_short | Biofunctionalization of Bacterial Cellulose with FucoPol Enhances Keratinocyte Activity and Wound Closure |
| title_sort | Biofunctionalization of Bacterial Cellulose with FucoPol Enhances Keratinocyte Activity and Wound Closure |
| topic | Bacterial cellulose Composite Hydrogel Polysaccharide Wound healing Biomaterials General Chemistry Biomedical Engineering Biochemistry, medical SDG 7 - Affordable and Clean Energy |
| topic_facet | Bacterial cellulose Composite Hydrogel Polysaccharide Wound healing Biomaterials General Chemistry Biomedical Engineering Biochemistry, medical SDG 7 - Affordable and Clean Energy |
| url | http://hdl.handle.net/10362/206327 |
| visible | 1 |
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