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Sustainable development of dual–templating molecularly imprinted polymers for biopurification

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Resumo:The demand for bio–based products is increasing, but the development of efficient purification processes is lagging. However, typically these processes are expensive, non–specific and/or lack of efficiency. Molecularly imprinted polymers (MIPs) are synthetic affinity materials able to mimic the molecular recognition ability of natural molecules, offering a cost–effective alternative to replace the commercial affinity–driven materials based on proteins and antibodies. In this work, MIPs were developed using supercritical carbon dioxide (scCO2) technology using two approaches: i) one–templating (O–MIPs), using L–leucine (LEU) as template, and ii) a dual–templating (D–MIPs), using LEU and L–lysine (LYS) as templates, to evaluate their potential in the molecular recognition of amino acids in simple and complex aqueous solutions. MIPs produced in scCO2 have already shown good performances in organic and aqueous solutions, for small and non–polar template molecules. Herein, their applicability is extended to amino acids but also proteins. MIPs were produced using 2–vinylpyridine (VP) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as crosslinker under scCO2 conditions. Polymers were obtained as white, ready–to–use dry powders. Their affinity performance was assessed by Static Binding Tests (SBTs) and Solid Phase Extraction (SPE) assays using different amino acids and proteins. The best SBT results was obtained by D–MIP with Qmax = 216 mg LEU + LYS/g D–MIP, and IFmax = 8.3. D–MIP also presented higher binding capacities to adsorb their templated–molecules by a dynamic process (SPE) (Qmax = 79 mg LEU + LYS/g D–MIP), and selectively bind their templates in a solution containing a protein (IFmax = 6.8). The green D–MIP provides a robust, tailor–made and sustainable alternative for biopurification processes.
Autores principais:Furtado, Ana I.
Outros Autores:Lobato, Diogo; Bonifácio, Vasco D. B.; Viveiros, Raquel; Casimiro, Teresa
Assunto:Biorecognition Bioseparation Dual–templating Molecularly imprinted polymers Solid–phase extraction Supercritical carbon dioxide Synthetic affinity materials Catalysis Electronic, Optical and Magnetic Materials Biomaterials Polymers and Plastics Colloid and Surface Chemistry Materials Chemistry
Ano:2025
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:Universidade Nova de Lisboa
Idioma:inglês
Origem:Repositório Institucional da UNL

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