Bibliographic Details
| Summary: | The design of small interfering RNA (siRNA) delivery materials showing efficacy in vivo is at the forefront of nanotherapeutics research. Polyurea (PURE-type) dendrimers are 'smart' biocompatible 3D polymers that unveil a dynamic and elegant back-folding mechanism involving hydrogen bonding between primary amines at the surface and tertiary amines and ureas at the core. Similarly, to a biological proton pump, they are able to automatically and reversibly transform their conformation in response to pH stimulus. Here, we show that PURE-G4 is a useful gene silencing platform showing no cellular toxicity. As a proof of concept we investigated the PURE-G4-siRNA dendriplex, which was shown to be an attractive platform with high transfection efficacy. The simplicity associated with the complexation of siRNA with polyurea dendrimers makes them a powerful tool for efficient cytosolic siRNA delivery. |
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| Main Authors: | Restani, Rita B. |
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| Other Authors: | Conde, João; Baptista, Pedro V.; Cidade, Maria Teresa; Bragança, Ana M.; Morgado, Jorge; Correia, Ilídio J.; Aguiar-Ricardo, Ana; Bonifácio, Vasco D. B. |
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| Subject: | General Chemistry General Chemical Engineering |
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| Year: | 2014 |
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| Country: | Portugal |
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| Document type: | article |
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| Access type: | open access |
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| Associated institution: | Universidade Nova de Lisboa |
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| Language: | English |
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| Origin: | Repositório Institucional da UNL |
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