Publicação
Bioinspired multilayer membranes as potential adhesive patches for skin wound healing
| Resumo: | Bioinspired and adhesive multilayer membranes are produced using the layer-by-layer (LbL) assembly of chitosan (CHT), alginate (ALG) and hyaluronic acid modified with dopamine (HA-DN). Freestanding multilayer membranes without DN are also produced as a control. The success of the synthesis of HA-DN was confirmed using UV-visible spectroscopy. Scanning electron microscopy images indicate that the surface of the DN-containing membranes is more porous than the control ones; they also present a higher average thickness value for the same number of CHT/ALG/CHT/HA(-DN) tetralayers (n = 100). Also, water uptake, mechanical strength and adhesion are enhanced with the introduction of DN moieties along the nano-layers. Besides, human dermal fibroblast viability, enhanced adhesion and proliferation were confirmed by immunofluorescence assays and by measuring both the metabolic activity and DNA content. Moreover, in vivo assays with such kinds of DN-containing multilayer membranes were performed; the application of these membranes in the treatment of dermal wounds induced in Wistar rats results in the highest decrease of inflammation of rat skin, compared with the control conditions. Overall, this investigation suggests that these mussel-inspired freestanding multilayer membranes may enhance either their mechanical performance or cellular adhesion and proliferation, leading to an improved wound healing process, being a promising material to restore the structural and functional properties of wounded skin. |
|---|---|
| Autores principais: | Sousa, M. P. |
| Outros Autores: | Neto, A. I.; Correia, T. R.; Miguel, S. P.; Matsusaki, M.; Correia, I. J.; Mano, J. F. |
| Assunto: | Adhesive patches Multilayer membranes Skin Wound healing |
| Ano: | 2018 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1877775825108992000 |
|---|---|
| author | Sousa, M. P. |
| author2 | Neto, A. I. Correia, T. R. Miguel, S. P. Matsusaki, M. Correia, I. J. Mano, J. F. |
| author2_role | author author author author author author |
| author_facet | Sousa, M. P. Neto, A. I. Correia, T. R. Miguel, S. P. Matsusaki, M. Correia, I. J. Mano, J. F. |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Sousa, M. P.\"},{\"Person.name\":\"Neto, A. I.\"},{\"Person.name\":\"Correia, T. R.\"},{\"Person.name\":\"Miguel, S. P.\"},{\"Person.name\":\"Matsusaki, M.\"},{\"Person.name\":\"Correia, I. J.\"},{\"Person.name\":\"Mano, J. F.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Sousa, M. P. Neto, A. I. Correia, T. R. Miguel, S. P. Matsusaki, M. Correia, I. J. Mano, J. F. |
| datacite.date.Accepted.fl_str_mv | 2018-05-01T00:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Adhesive patches Multilayer membranes Skin Wound healing |
| datacite.titles.title.fl_str_mv | Bioinspired multilayer membranes as potential adhesive patches for skin wound healing |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Sousa, M. P. Neto, A. I. Correia, T. R. Miguel, S. P. Matsusaki, M. Correia, I. J. Mano, J. F. |
| dc.date.Accepted.fl_str_mv | 2018-05-01T00:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/58832 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Royal Society of Chemistry |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Adhesive patches Multilayer membranes Skin Wound healing |
| dc.title.fl_str_mv | Bioinspired multilayer membranes as potential adhesive patches for skin wound healing |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Bioinspired and adhesive multilayer membranes are produced using the layer-by-layer (LbL) assembly of chitosan (CHT), alginate (ALG) and hyaluronic acid modified with dopamine (HA-DN). Freestanding multilayer membranes without DN are also produced as a control. The success of the synthesis of HA-DN was confirmed using UV-visible spectroscopy. Scanning electron microscopy images indicate that the surface of the DN-containing membranes is more porous than the control ones; they also present a higher average thickness value for the same number of CHT/ALG/CHT/HA(-DN) tetralayers (n = 100). Also, water uptake, mechanical strength and adhesion are enhanced with the introduction of DN moieties along the nano-layers. Besides, human dermal fibroblast viability, enhanced adhesion and proliferation were confirmed by immunofluorescence assays and by measuring both the metabolic activity and DNA content. Moreover, in vivo assays with such kinds of DN-containing multilayer membranes were performed; the application of these membranes in the treatment of dermal wounds induced in Wistar rats results in the highest decrease of inflammation of rat skin, compared with the control conditions. Overall, this investigation suggests that these mussel-inspired freestanding multilayer membranes may enhance either their mechanical performance or cellular adhesion and proliferation, leading to an improved wound healing process, being a promising material to restore the structural and functional properties of wounded skin. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| id | rum_ece0fa5f32c41dc40693102d5b7f5b23 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/58832 |
| inst_facet_str | urn:organizationAcronym:repositorium{{{_:::_}}}Universidade do Minho |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/58832 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Sousa, M. P. Neto, A. I. Correia, T. R. Miguel, S. P. Matsusaki, M. Correia, I. J. Mano, J. F. |
| publishDate | 2018 |
| publisher.none.fl_str_mv | Royal Society of Chemistry |
| repo_facet_str | urn:repositoryAcronym:rum{{{_:::_}}}RepositóriUM - Universidade do Minho |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engRoyal Society of ChemistryporBioinspired and adhesive multilayer membranes are produced using the layer-by-layer (LbL) assembly of chitosan (CHT), alginate (ALG) and hyaluronic acid modified with dopamine (HA-DN). Freestanding multilayer membranes without DN are also produced as a control. The success of the synthesis of HA-DN was confirmed using UV-visible spectroscopy. Scanning electron microscopy images indicate that the surface of the DN-containing membranes is more porous than the control ones; they also present a higher average thickness value for the same number of CHT/ALG/CHT/HA(-DN) tetralayers (n = 100). Also, water uptake, mechanical strength and adhesion are enhanced with the introduction of DN moieties along the nano-layers. Besides, human dermal fibroblast viability, enhanced adhesion and proliferation were confirmed by immunofluorescence assays and by measuring both the metabolic activity and DNA content. Moreover, in vivo assays with such kinds of DN-containing multilayer membranes were performed; the application of these membranes in the treatment of dermal wounds induced in Wistar rats results in the highest decrease of inflammation of rat skin, compared with the control conditions. Overall, this investigation suggests that these mussel-inspired freestanding multilayer membranes may enhance either their mechanical performance or cellular adhesion and proliferation, leading to an improved wound healing process, being a promising material to restore the structural and functional properties of wounded skin.application/pdfporBioinspired multilayer membranes as potential adhesive patches for skin wound healingSousa, M. P.Neto, A. I.Correia, T. R.Miguel, S. P.Matsusaki, M.Correia, I. J.Mano, J. F.HostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONSousa M. P., Neto A. I., Correia T. R., Miguel S. P., Matsusaki M., Correia I. J., Mano J. F. Bioinspired multilayer membranes as potential adhesive patches for skin wound healing, Biomaterials Science, Vol. 6, pp. 1962–1975, doi:10.1039/C8BM00319J, 2018PMID29850674ISSNIsPartOf2047-4849DOIIsPartOf10.1039/C8BM00319J2018-052018-05-01T00:00:00ZHandlehttps://hdl.handle.net/1822/58832http://purl.org/coar/access_right/c_abf2open accessAdhesive patchesMultilayer membranesSkinWound healing2609564 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdfhttps://repositorium.uminho.pt/bitstreams/cab3014f-9b43-4e63-b9ad-46ee0956565c/download |
| spellingShingle | Bioinspired multilayer membranes as potential adhesive patches for skin wound healing Sousa, M. P. Adhesive patches Multilayer membranes Skin Wound healing |
| status | SINGLETON |
| subject.fl_str_mv | Adhesive patches Multilayer membranes Skin Wound healing |
| title | Bioinspired multilayer membranes as potential adhesive patches for skin wound healing |
| title_full | Bioinspired multilayer membranes as potential adhesive patches for skin wound healing |
| title_fullStr | Bioinspired multilayer membranes as potential adhesive patches for skin wound healing |
| title_full_unstemmed | Bioinspired multilayer membranes as potential adhesive patches for skin wound healing |
| title_short | Bioinspired multilayer membranes as potential adhesive patches for skin wound healing |
| title_sort | Bioinspired multilayer membranes as potential adhesive patches for skin wound healing |
| topic | Adhesive patches Multilayer membranes Skin Wound healing |
| topic_facet | Adhesive patches Multilayer membranes Skin Wound healing |
| url | https://hdl.handle.net/1822/58832 |
| visible | 1 |
Atividades financiadas
Não foram encontrados projetos financiados.