Publicação
Fucoidan hydrogels photo-cross-linked with visible radiation as matrices for cell culture
| Resumo: | Algae are abundant sources of bioactive components with extensive therapeutic properties, receiving much interest in recent years. The research on marine brown algae, namely one of its polysaccharide-fucoidan, has increased exponentially. Fucoidan is a sulfated cell-wall polysaccharide with several reported biological properties including anticancer, antivirus, anticoagulant, antioxidant and anti-inflammatory effects. In this study, fucoidan was functionalized by grafting methacrylic groups in the chain backbone, photo-cross-linkable under visible light to obtain biodegradable structures for tissue engineering. The functionalization reaction was carried out by concentrations (8 and 12%) of methacrylic anhydride (MA). The modified fucoidan (MFu) was characterized by FTIR and 1HNMR spectroscopy to confirm the functionalization. Further, modified fucoidan was photo-cross-linked under visible light and using superhydrophobic surfaces, to obtain spherical particles with controlled geometries benefiting from the high repellence of the surfaces. When using higher concentrations of MA, the particles were observed to exhibit a smaller average diameter. Moreover, the behavior of L929 mouse fibroblast-like cells was evaluated when cultured in contact with photo-cross-linked particles was investigated, being observed up to 14 days in culture. The photo-cross-linking of MFu under visible light enables thus the formation of particles here suggested as potentially relevant in a wide range of biomedical applications. |
|---|---|
| Autores principais: | Reys, L. L. |
| Outros Autores: | Silva, Simone Santos; Costa, Diana Pereira Soares; Oliveira, Nuno M.; Mano, J. F.; Reis, R. L.; Silva, Tiago José Quinteiros Lopes Henriques |
| Assunto: | Fucoidan Hydrogel Marine biomaterials Methacrylation Photo-cross-linking Tissue engineering |
| Ano: | 2016 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1877775775126519808 |
|---|---|
| author | Reys, L. L. |
| author2 | Silva, Simone Santos Costa, Diana Pereira Soares Oliveira, Nuno M. Mano, J. F. Reis, R. L. Silva, Tiago José Quinteiros Lopes Henriques |
| author2_role | author author author author author author |
| author_facet | Reys, L. L. Silva, Simone Santos Costa, Diana Pereira Soares Oliveira, Nuno M. Mano, J. F. Reis, R. L. Silva, Tiago José Quinteiros Lopes Henriques |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Reys, L. L.\"},{\"Person.name\":\"Silva, Simone Santos\"},{\"Person.name\":\"Costa, Diana Pereira Soares\"},{\"Person.name\":\"Oliveira, Nuno M.\"},{\"Person.name\":\"Mano, J. F.\"},{\"Person.name\":\"Reis, R. L.\"},{\"Person.name\":\"Silva, Tiago José Quinteiros Lopes Henriques\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Reys, L. L. Silva, Simone Santos Costa, Diana Pereira Soares Oliveira, Nuno M. Mano, J. F. Reis, R. L. Silva, Tiago José Quinteiros Lopes Henriques |
| datacite.date.Accepted.fl_str_mv | 2016-05-01T00:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Fucoidan Hydrogel Marine biomaterials Methacrylation Photo-cross-linking Tissue engineering |
| datacite.titles.title.fl_str_mv | Fucoidan hydrogels photo-cross-linked with visible radiation as matrices for cell culture |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Reys, L. L. Silva, Simone Santos Costa, Diana Pereira Soares Oliveira, Nuno M. Mano, J. F. Reis, R. L. Silva, Tiago José Quinteiros Lopes Henriques |
| dc.date.Accepted.fl_str_mv | 2016-05-01T00:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/44384 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | ACS Publications |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Fucoidan Hydrogel Marine biomaterials Methacrylation Photo-cross-linking Tissue engineering |
| dc.title.fl_str_mv | Fucoidan hydrogels photo-cross-linked with visible radiation as matrices for cell culture |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Algae are abundant sources of bioactive components with extensive therapeutic properties, receiving much interest in recent years. The research on marine brown algae, namely one of its polysaccharide-fucoidan, has increased exponentially. Fucoidan is a sulfated cell-wall polysaccharide with several reported biological properties including anticancer, antivirus, anticoagulant, antioxidant and anti-inflammatory effects. In this study, fucoidan was functionalized by grafting methacrylic groups in the chain backbone, photo-cross-linkable under visible light to obtain biodegradable structures for tissue engineering. The functionalization reaction was carried out by concentrations (8 and 12%) of methacrylic anhydride (MA). The modified fucoidan (MFu) was characterized by FTIR and 1HNMR spectroscopy to confirm the functionalization. Further, modified fucoidan was photo-cross-linked under visible light and using superhydrophobic surfaces, to obtain spherical particles with controlled geometries benefiting from the high repellence of the surfaces. When using higher concentrations of MA, the particles were observed to exhibit a smaller average diameter. Moreover, the behavior of L929 mouse fibroblast-like cells was evaluated when cultured in contact with photo-cross-linked particles was investigated, being observed up to 14 days in culture. The photo-cross-linking of MFu under visible light enables thus the formation of particles here suggested as potentially relevant in a wide range of biomedical applications. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| id | rum_e22b2e6ed2086c81288417d6133e393a |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/44384 |
| inst_facet_str | urn:organizationAcronym:repositorium{{{_:::_}}}Universidade do Minho |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/44384 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Reys, L. L. Silva, Simone Santos Costa, Diana Pereira Soares Oliveira, Nuno M. Mano, J. F. Reis, R. L. Silva, Tiago José Quinteiros Lopes Henriques |
| publishDate | 2016 |
| publisher.none.fl_str_mv | ACS Publications |
| repo_facet_str | urn:repositoryAcronym:rum{{{_:::_}}}RepositóriUM - Universidade do Minho |
| reponame_str | RepositóriUM - Universidade do Minho |
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| spelling | engACS PublicationsporAlgae are abundant sources of bioactive components with extensive therapeutic properties, receiving much interest in recent years. The research on marine brown algae, namely one of its polysaccharide-fucoidan, has increased exponentially. Fucoidan is a sulfated cell-wall polysaccharide with several reported biological properties including anticancer, antivirus, anticoagulant, antioxidant and anti-inflammatory effects. In this study, fucoidan was functionalized by grafting methacrylic groups in the chain backbone, photo-cross-linkable under visible light to obtain biodegradable structures for tissue engineering. The functionalization reaction was carried out by concentrations (8 and 12%) of methacrylic anhydride (MA). The modified fucoidan (MFu) was characterized by FTIR and 1HNMR spectroscopy to confirm the functionalization. Further, modified fucoidan was photo-cross-linked under visible light and using superhydrophobic surfaces, to obtain spherical particles with controlled geometries benefiting from the high repellence of the surfaces. When using higher concentrations of MA, the particles were observed to exhibit a smaller average diameter. Moreover, the behavior of L929 mouse fibroblast-like cells was evaluated when cultured in contact with photo-cross-linked particles was investigated, being observed up to 14 days in culture. The photo-cross-linking of MFu under visible light enables thus the formation of particles here suggested as potentially relevant in a wide range of biomedical applications.application/pdfporFucoidan hydrogels photo-cross-linked with visible radiation as matrices for cell cultureReys, L. L.Silva, Simone SantosCosta, Diana Pereira SoaresOliveira, Nuno M.Mano, J. F.Reis, R. L.Silva, Tiago José Quinteiros Lopes HenriquesHostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONReys L. L., Silva S. S., Soares da Costa D., Oliveira N. M., Mano J. F., Reis R. L., Silva T. H. Fucoidan hydrogels photo-cross-linked with visible radiation as matrices for cell culture, ACS Biomaterials Science & Engineering, Vol. 2, Issue 7, pp. 1151–1161, doi:10.1021/acsbiomaterials.6b00180, 2016ISSNIsPartOf2373-9878DOIIsPartOf10.1021/acsbiomaterials.6b001802016-052016-05-01T00:00:00ZHandlehttps://hdl.handle.net/1822/44384http://purl.org/coar/access_right/c_16ecrestricted accessFucoidanHydrogelMarine biomaterialsMethacrylationPhoto-cross-linkingTissue engineering5440455 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdfhttps://repositorium.uminho.pt/bitstreams/a8a99708-7ca4-4acc-b2e5-39ec7accee56/download |
| spellingShingle | Fucoidan hydrogels photo-cross-linked with visible radiation as matrices for cell culture Reys, L. L. Fucoidan Hydrogel Marine biomaterials Methacrylation Photo-cross-linking Tissue engineering |
| status | SINGLETON |
| subject.fl_str_mv | Fucoidan Hydrogel Marine biomaterials Methacrylation Photo-cross-linking Tissue engineering |
| title | Fucoidan hydrogels photo-cross-linked with visible radiation as matrices for cell culture |
| title_full | Fucoidan hydrogels photo-cross-linked with visible radiation as matrices for cell culture |
| title_fullStr | Fucoidan hydrogels photo-cross-linked with visible radiation as matrices for cell culture |
| title_full_unstemmed | Fucoidan hydrogels photo-cross-linked with visible radiation as matrices for cell culture |
| title_short | Fucoidan hydrogels photo-cross-linked with visible radiation as matrices for cell culture |
| title_sort | Fucoidan hydrogels photo-cross-linked with visible radiation as matrices for cell culture |
| topic | Fucoidan Hydrogel Marine biomaterials Methacrylation Photo-cross-linking Tissue engineering |
| topic_facet | Fucoidan Hydrogel Marine biomaterials Methacrylation Photo-cross-linking Tissue engineering |
| url | https://hdl.handle.net/1822/44384 |
| visible | 1 |
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