Publicação
New perspectives of Juglans regia L. phytochemicals against Candida species
| Resumo: | Opportunistic fungal infections have deserved special relevance in the last decades, presenting itself, a serious problem in terms of public health. Despite Candida albicans was considered the main agent responsible for those infections, other non-albicans Candida species have also been described in the last years [1-3]. Most of the species are susceptible to antimicrobial drugs, but recently it has been observed a growing number of microorganisms with drug resistance. Therefore, the discover/use of alternative therapies is crucial [4]. Juglans regia L. (walnut) leaves are commonly used in traditional medicine as antiseptic, antimicrobial and anti-inflammatory [5]; those benefits could be related with its richness in phenolic compounds [6]. In the present work, the antifungal potential of the hydroalcoholic extract prepared from walnut leaves was evaluated against a total of nineteen Candida strains (from the species: C.albicans, C.glabrata, C.parapsilosis and C.tropicalis), using the disc diffusion halo assay. All the tested strains were sensible to the plant extract. The obtained values of the inhibitory zones ranged between 0.9-1.4 cm, being the halo maintained after 48h. The observed antifungal activity is certainly related to the phenolic compounds previously determined in the extract [6]: five phenolic acid derivatives- caffeoylquinic and p-coumaroylquinic acid derivatives, two dimers and one trimer of procyanidins, twelve flavonols- quercetin, myricetin and kaempferol derivatives, and five taxifolin O-pentoside isomers; 3-O-caffeoylquinic acids and quercetin O-pentoside were the main phenolic compounds. Further studies are necessaries in order to elucidate the most active compounds and the specific role of each one. REFERENCES [1] Kim J, Sudbery P. (2011) J Microbiol. 49, 171-177. [2] Tsai P-W, Chen Y-T, Hsu P-C, Lan C-Y. (2013) BioMedicine. 3, 51–64. [3] Vázquez-González D, Perusquía-Ortiz AM, Hundeiker M, Bonifaz A. (2013) J Ger Soc Dermatology. 11, 381–94. [4] Kanafani ZA, Perfect JR. (2008) Clin Infect Dis - Antimicrob Resist. 46, 120–128. [5] Murray MT. (2004) 2nd ed. New York, NY. Random House. [6] Santos A, Barros L, Calhelha RC, Dueñas M, Carvalho AM, Santos-Buelga C, Ferreira ICFR. (2013) Ind Crops Prod. 51, 430-436. |
|---|---|
| Autores principais: | Martins, Natália |
| Outros Autores: | Barros, Lillian; Carvalho, Ana Maria; Silva, Sónia Carina; Henriques, Mariana; Ferreira, Isabel C. F. R. |
| Assunto: | Antifungal activity Juglans regia L. Hydroalcoholic extract Candida species |
| Ano: | 2014 |
| País: | Portugal |
| Tipo de documento: | outro |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1877776315885551616 |
|---|---|
| author | Martins, Natália |
| author2 | Barros, Lillian Carvalho, Ana Maria Silva, Sónia Carina Henriques, Mariana Ferreira, Isabel C. F. R. |
| author2_role | author author author author author |
| author_facet | Martins, Natália Barros, Lillian Carvalho, Ana Maria Silva, Sónia Carina Henriques, Mariana Ferreira, Isabel C. F. R. |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Martins, Natália\"},{\"Person.name\":\"Barros, Lillian\"},{\"Person.name\":\"Carvalho, Ana Maria\"},{\"Person.name\":\"Silva, Sónia Carina\"},{\"Person.name\":\"Henriques, Mariana\"},{\"Person.name\":\"Ferreira, Isabel C. F. R.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Martins, Natália Barros, Lillian Carvalho, Ana Maria Silva, Sónia Carina Henriques, Mariana Ferreira, Isabel C. F. R. |
| datacite.date.Accepted.fl_str_mv | 2014-01-01T00:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Antifungal activity Juglans regia L. Hydroalcoholic extract Candida species |
| datacite.titles.title.fl_str_mv | New perspectives of Juglans regia L. phytochemicals against Candida species |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Martins, Natália Barros, Lillian Carvalho, Ana Maria Silva, Sónia Carina Henriques, Mariana Ferreira, Isabel C. F. R. |
| dc.date.Accepted.fl_str_mv | 2014-01-01T00:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/31683 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | European Society of Clinical Microbiology and Infectious Diseases (ESCMID) |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Antifungal activity Juglans regia L. Hydroalcoholic extract Candida species |
| dc.title.fl_str_mv | New perspectives of Juglans regia L. phytochemicals against Candida species |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_1843 |
| description | Opportunistic fungal infections have deserved special relevance in the last decades, presenting itself, a serious problem in terms of public health. Despite Candida albicans was considered the main agent responsible for those infections, other non-albicans Candida species have also been described in the last years [1-3]. Most of the species are susceptible to antimicrobial drugs, but recently it has been observed a growing number of microorganisms with drug resistance. Therefore, the discover/use of alternative therapies is crucial [4]. Juglans regia L. (walnut) leaves are commonly used in traditional medicine as antiseptic, antimicrobial and anti-inflammatory [5]; those benefits could be related with its richness in phenolic compounds [6]. In the present work, the antifungal potential of the hydroalcoholic extract prepared from walnut leaves was evaluated against a total of nineteen Candida strains (from the species: C.albicans, C.glabrata, C.parapsilosis and C.tropicalis), using the disc diffusion halo assay. All the tested strains were sensible to the plant extract. The obtained values of the inhibitory zones ranged between 0.9-1.4 cm, being the halo maintained after 48h. The observed antifungal activity is certainly related to the phenolic compounds previously determined in the extract [6]: five phenolic acid derivatives- caffeoylquinic and p-coumaroylquinic acid derivatives, two dimers and one trimer of procyanidins, twelve flavonols- quercetin, myricetin and kaempferol derivatives, and five taxifolin O-pentoside isomers; 3-O-caffeoylquinic acids and quercetin O-pentoside were the main phenolic compounds. Further studies are necessaries in order to elucidate the most active compounds and the specific role of each one. REFERENCES [1] Kim J, Sudbery P. (2011) J Microbiol. 49, 171-177. [2] Tsai P-W, Chen Y-T, Hsu P-C, Lan C-Y. (2013) BioMedicine. 3, 51–64. [3] Vázquez-González D, Perusquía-Ortiz AM, Hundeiker M, Bonifaz A. (2013) J Ger Soc Dermatology. 11, 381–94. [4] Kanafani ZA, Perfect JR. (2008) Clin Infect Dis - Antimicrob Resist. 46, 120–128. [5] Murray MT. (2004) 2nd ed. New York, NY. Random House. [6] Santos A, Barros L, Calhelha RC, Dueñas M, Carvalho AM, Santos-Buelga C, Ferreira ICFR. (2013) Ind Crops Prod. 51, 430-436. |
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| eu_rights_str_mv | openAccess |
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| identifier.url.fl_str_mv | https://hdl.handle.net/1822/31683 |
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| language | eng |
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| oai_identifier_str | oai:repositorium.uminho.pt:1822/31683 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Martins, Natália Barros, Lillian Carvalho, Ana Maria Silva, Sónia Carina Henriques, Mariana Ferreira, Isabel C. F. R. |
| publishDate | 2014 |
| publisher.none.fl_str_mv | European Society of Clinical Microbiology and Infectious Diseases (ESCMID) |
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| spelling | engEuropean Society of Clinical Microbiology and Infectious Diseases (ESCMID)Opportunistic fungal infections have deserved special relevance in the last decades, presenting itself, a serious problem in terms of public health. Despite Candida albicans was considered the main agent responsible for those infections, other non-albicans Candida species have also been described in the last years [1-3]. Most of the species are susceptible to antimicrobial drugs, but recently it has been observed a growing number of microorganisms with drug resistance. Therefore, the discover/use of alternative therapies is crucial [4]. Juglans regia L. (walnut) leaves are commonly used in traditional medicine as antiseptic, antimicrobial and anti-inflammatory [5]; those benefits could be related with its richness in phenolic compounds [6]. In the present work, the antifungal potential of the hydroalcoholic extract prepared from walnut leaves was evaluated against a total of nineteen Candida strains (from the species: C.albicans, C.glabrata, C.parapsilosis and C.tropicalis), using the disc diffusion halo assay. All the tested strains were sensible to the plant extract. The obtained values of the inhibitory zones ranged between 0.9-1.4 cm, being the halo maintained after 48h. The observed antifungal activity is certainly related to the phenolic compounds previously determined in the extract [6]: five phenolic acid derivatives- caffeoylquinic and p-coumaroylquinic acid derivatives, two dimers and one trimer of procyanidins, twelve flavonols- quercetin, myricetin and kaempferol derivatives, and five taxifolin O-pentoside isomers; 3-O-caffeoylquinic acids and quercetin O-pentoside were the main phenolic compounds. Further studies are necessaries in order to elucidate the most active compounds and the specific role of each one. REFERENCES [1] Kim J, Sudbery P. (2011) J Microbiol. 49, 171-177. [2] Tsai P-W, Chen Y-T, Hsu P-C, Lan C-Y. (2013) BioMedicine. 3, 51–64. [3] Vázquez-González D, Perusquía-Ortiz AM, Hundeiker M, Bonifaz A. (2013) J Ger Soc Dermatology. 11, 381–94. [4] Kanafani ZA, Perfect JR. (2008) Clin Infect Dis - Antimicrob Resist. 46, 120–128. [5] Murray MT. (2004) 2nd ed. New York, NY. Random House. [6] Santos A, Barros L, Calhelha RC, Dueñas M, Carvalho AM, Santos-Buelga C, Ferreira ICFR. (2013) Ind Crops Prod. 51, 430-436.application/pdfporNew perspectives of Juglans regia L. phytochemicals against Candida speciesMartins, NatáliaBarros, LillianCarvalho, Ana MariaSilva, Sónia CarinaHenriques, MarianaFerreira, Isabel C. F. R.HostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONMartins, Natália; Barros, Lillian; Carvalho, Ana Maria; Silva, Sónia Carina; Henriques, Mariana; Ferreira, Isabel C. F. R., New perspectives of Juglans regia L. phytochemicals against Candida species. TEMPH2014 - Trends in Environmental Microbiology for Public Health. Lisboa, Portugal, Sep. 18-21, European Society of Clinical Microbiology and Infectious Diseases - ESCMID, 2014.20142014-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/31683http://purl.org/coar/access_right/c_abf2open accessAntifungal activityJuglans regia L.Hydroalcoholic extractCandida species409113 bytesother research producthttp://purl.org/coar/resource_type/c_1843otherhttp://purl.org/coar/access_right/c_abf2application/pdfhttps://repositorium.uminho.pt/bitstreams/4ae1e089-8ded-40aa-8ef4-9ffd374c06c6/download |
| spellingShingle | New perspectives of Juglans regia L. phytochemicals against Candida species Martins, Natália Antifungal activity Juglans regia L. Hydroalcoholic extract Candida species |
| status | SINGLETON |
| subject.fl_str_mv | Antifungal activity Juglans regia L. Hydroalcoholic extract Candida species |
| title | New perspectives of Juglans regia L. phytochemicals against Candida species |
| title_full | New perspectives of Juglans regia L. phytochemicals against Candida species |
| title_fullStr | New perspectives of Juglans regia L. phytochemicals against Candida species |
| title_full_unstemmed | New perspectives of Juglans regia L. phytochemicals against Candida species |
| title_short | New perspectives of Juglans regia L. phytochemicals against Candida species |
| title_sort | New perspectives of Juglans regia L. phytochemicals against Candida species |
| topic | Antifungal activity Juglans regia L. Hydroalcoholic extract Candida species |
| topic_facet | Antifungal activity Juglans regia L. Hydroalcoholic extract Candida species |
| url | https://hdl.handle.net/1822/31683 |
| visible | 1 |
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