Publication
Protocols for decellularization of human amniotic membrane
| Summary: | Human amniotic membrane (HAM) has been used as a very promising biological-based product in health centers, especially for skin and cornea wound healing applications. The excellent properties of this membrane make it a potential candidate in treatment of various skin injuries such as bedsores, burn wounds and diabetic ulcers. Such properties are cytobiocompatibility, a structure very similar to normal skin composed of extracellular matrix (ECM) proteins, various growth factors involved in normal wound healing process and antibacterial agents. HAM contains epithelial cells, fibroblasts and mesenchymal stem cells. Therefore, the successful decellularization of HAM with minimal negative effects on its ECM components is very important to avoid graft rejection and shows improved performance. To date, several approaches have been conducted for decellularization of HAM, which is mainly based on enzyme-, detergent- or mechanical procedures with various ranges of success. Here, we describe a systematic detergent based decellularization protocol as main protocol. We also explain the enzyme- and mechanical based methods as the alternative protocols for decellularization of HAM. |
|---|---|
| Main Authors: | Khosravimelal, S. |
| Other Authors: | Momeni, M.; Gholipur, M.; Kundu, Subhas C; Gholipourmalekabadi, M. |
| Subject: | Decellurization. Protocol human amniotic membrane Amniotic membrane Decellularization Denudation Detergent-based decellularization Tissue engineering Wound dressing |
| Year: | 2020 |
| Country: | Portugal |
| Document type: | book part |
| Access type: | restricted access |
| Associated institution: | Universidade do Minho |
| Language: | English |
| Origin: | RepositóriUM - Universidade do Minho |
| _version_ | 1877775872067371008 |
|---|---|
| author | Khosravimelal, S. |
| author2 | Momeni, M. Gholipur, M. Kundu, Subhas C Gholipourmalekabadi, M. |
| author2_role | author author author author |
| author_facet | Khosravimelal, S. Momeni, M. Gholipur, M. Kundu, Subhas C Gholipourmalekabadi, M. |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Khosravimelal, S.\"},{\"Person.name\":\"Momeni, M.\"},{\"Person.name\":\"Gholipur, M.\"},{\"Person.name\":\"Kundu, Subhas C\"},{\"Person.name\":\"Gholipourmalekabadi, M.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Khosravimelal, S. Momeni, M. Gholipur, M. Kundu, Subhas C Gholipourmalekabadi, M. |
| datacite.date.Accepted.fl_str_mv | 2020-04-01T00:00:00Z |
| datacite.date.embargoed.fl_str_mv | 10000-01-01T00:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Decellurization. Protocol human amniotic membrane Amniotic membrane Decellularization Denudation Detergent-based decellularization Tissue engineering Wound dressing |
| datacite.titles.title.fl_str_mv | Protocols for decellularization of human amniotic membrane |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Khosravimelal, S. Momeni, M. Gholipur, M. Kundu, Subhas C Gholipourmalekabadi, M. |
| dc.date.Accepted.fl_str_mv | 2020-04-01T00:00:00Z |
| dc.date.embargoed.fl_str_mv | 10000-01-01T00:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/66341 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier B.V. |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Decellurization. Protocol human amniotic membrane Amniotic membrane Decellularization Denudation Detergent-based decellularization Tissue engineering Wound dressing |
| dc.title.fl_str_mv | Protocols for decellularization of human amniotic membrane |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_3248 |
| description | Human amniotic membrane (HAM) has been used as a very promising biological-based product in health centers, especially for skin and cornea wound healing applications. The excellent properties of this membrane make it a potential candidate in treatment of various skin injuries such as bedsores, burn wounds and diabetic ulcers. Such properties are cytobiocompatibility, a structure very similar to normal skin composed of extracellular matrix (ECM) proteins, various growth factors involved in normal wound healing process and antibacterial agents. HAM contains epithelial cells, fibroblasts and mesenchymal stem cells. Therefore, the successful decellularization of HAM with minimal negative effects on its ECM components is very important to avoid graft rejection and shows improved performance. To date, several approaches have been conducted for decellularization of HAM, which is mainly based on enzyme-, detergent- or mechanical procedures with various ranges of success. Here, we describe a systematic detergent based decellularization protocol as main protocol. We also explain the enzyme- and mechanical based methods as the alternative protocols for decellularization of HAM. |
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| identifier.url.fl_str_mv | https://hdl.handle.net/1822/66341 |
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| language | eng |
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| person_str_mv | Khosravimelal, S. Momeni, M. Gholipur, M. Kundu, Subhas C Gholipourmalekabadi, M. |
| publishDate | 2020 |
| publisher.none.fl_str_mv | Elsevier B.V. |
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| spelling | engElsevier B.V.porHuman amniotic membrane (HAM) has been used as a very promising biological-based product in health centers, especially for skin and cornea wound healing applications. The excellent properties of this membrane make it a potential candidate in treatment of various skin injuries such as bedsores, burn wounds and diabetic ulcers. Such properties are cytobiocompatibility, a structure very similar to normal skin composed of extracellular matrix (ECM) proteins, various growth factors involved in normal wound healing process and antibacterial agents. HAM contains epithelial cells, fibroblasts and mesenchymal stem cells. Therefore, the successful decellularization of HAM with minimal negative effects on its ECM components is very important to avoid graft rejection and shows improved performance. To date, several approaches have been conducted for decellularization of HAM, which is mainly based on enzyme-, detergent- or mechanical procedures with various ranges of success. Here, we describe a systematic detergent based decellularization protocol as main protocol. We also explain the enzyme- and mechanical based methods as the alternative protocols for decellularization of HAM.application/pdfporProtocols for decellularization of human amniotic membraneKhosravimelal, S.Momeni, M.Gholipur, M.Kundu, Subhas CGholipourmalekabadi, M.HostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONKhosravimelal S., Momeni M., Gholipur M., Kundu S. C., Gholipourmalekabadi M. Protocols for decellularization of human amniotic membrane, Methods in Cell Biology –Cell-derived Matrices, Vol. 157, Issue Part B, pp. 37-48, 0091-679X, 2020PMID32334719ISBNIsPartOf978-0-12-820174-9ISSNIsPartOf0091-679XDOIIsPartOf10.1016/bs.mcb.2019.11.0042020-042020-04-01T00:00:00Z10000-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/66341http://purl.org/coar/access_right/c_16ecrestricted accessDecellurization. Protocolhuman amniotic membraneAmniotic membraneDecellularizationDenudationDetergent-based decellularizationTissue engineeringWound dressing2158768 bytesliteraturehttp://purl.org/coar/resource_type/c_3248book parthttp://purl.org/coar/access_right/c_f1cfapplication/pdfhttps://repositorium.uminho.pt/bitstreams/0f8aa5fa-b569-4f1c-8a71-8afebc819dba/download |
| spellingShingle | Protocols for decellularization of human amniotic membrane Khosravimelal, S. Decellurization. Protocol human amniotic membrane Amniotic membrane Decellularization Denudation Detergent-based decellularization Tissue engineering Wound dressing |
| status | SINGLETON |
| subject.fl_str_mv | Decellurization. Protocol human amniotic membrane Amniotic membrane Decellularization Denudation Detergent-based decellularization Tissue engineering Wound dressing |
| title | Protocols for decellularization of human amniotic membrane |
| title_full | Protocols for decellularization of human amniotic membrane |
| title_fullStr | Protocols for decellularization of human amniotic membrane |
| title_full_unstemmed | Protocols for decellularization of human amniotic membrane |
| title_short | Protocols for decellularization of human amniotic membrane |
| title_sort | Protocols for decellularization of human amniotic membrane |
| topic | Decellurization. Protocol human amniotic membrane Amniotic membrane Decellularization Denudation Detergent-based decellularization Tissue engineering Wound dressing |
| topic_facet | Decellurization. Protocol human amniotic membrane Amniotic membrane Decellularization Denudation Detergent-based decellularization Tissue engineering Wound dressing |
| url | https://hdl.handle.net/1822/66341 |
| visible | 1 |
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