Publication
Phospholipid‐induced silk fibroin hydrogels and their potential as cell carriers for tissue regeneration
| Summary: | Silk fibroin (SF) hydrogels can be obtained via self‐assembly, but this process takes several days or weeks, being unfeasible to produce cell carrier hydrogels. In this work, a phospholipid, namely, 1,2‐dimyristoyl‐sn‐glycero‐3‐phospho‐(1′‐rac‐glycerol) sodium salt (DMPG), was used to induce and accelerate the gelation process of SF solutions. Due to the amphipathic nature and negative charge of DMPG, electrostatic and hydrophobic interactions between the phospholipids and SF chains will occur, inducing the structural transition of SF chains to the beta sheet and consequently a rapid gel formation is observed (less than 50 min). Moreover, the gelation time can be controlled by varying the lipid concentration. To assess the potential of the hydrogels as cell carriers, several mammalian cell lines, including L929, NIH/3T3, SaOS‐2, and CaSki, were encapsulated into the hydrogel. The silk‐based hydrogels supported the normal growth of fibroblasts, corroborating their cytocompatibility. Interestingly, an inhibition in the growth of cancer‐derived cell lines was observed. Therefore, DMPG‐induced SF hydrogels can be successfully used as a 3D platform for in situ cell encapsulation, opening promising opportunities in biomedical applications, such as in cell therapies and tissue regeneration. |
|---|---|
| Main Authors: | Laomeephol, Chavee |
| Other Authors: | Guedes, Marta Cristina Alves Moreira; Ferreira, Helena Susana Costa Machado; Reis, R. L.; Kanokpanont, Sorada; Damrongsakkul, Siriporn; Neves, N. M. |
| Subject: | Cell encapsulation Cytocompatibility DMPG Gelation time Hydrogel Silk Fibroin |
| Year: | 2020 |
| Country: | Portugal |
| Document type: | article |
| Access type: | open access |
| Associated institution: | Universidade do Minho |
| Language: | English |
| Origin: | RepositóriUM - Universidade do Minho |
| _version_ | 1877775870912888832 |
|---|---|
| author | Laomeephol, Chavee |
| author2 | Guedes, Marta Cristina Alves Moreira Ferreira, Helena Susana Costa Machado Reis, R. L. Kanokpanont, Sorada Damrongsakkul, Siriporn Neves, N. M. |
| author2_role | author author author author author author |
| author_facet | Laomeephol, Chavee Guedes, Marta Cristina Alves Moreira Ferreira, Helena Susana Costa Machado Reis, R. L. Kanokpanont, Sorada Damrongsakkul, Siriporn Neves, N. M. |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Laomeephol, Chavee\"},{\"Person.name\":\"Guedes, Marta Cristina Alves Moreira\"},{\"Person.name\":\"Ferreira, Helena Susana Costa Machado\"},{\"Person.name\":\"Reis, R. L.\"},{\"Person.name\":\"Kanokpanont, Sorada\"},{\"Person.name\":\"Damrongsakkul, Siriporn\"},{\"Person.name\":\"Neves, N. M.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Laomeephol, Chavee Guedes, Marta Cristina Alves Moreira Ferreira, Helena Susana Costa Machado Reis, R. L. Kanokpanont, Sorada Damrongsakkul, Siriporn Neves, N. M. |
| datacite.date.Accepted.fl_str_mv | 2020-01-01T00:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Cell encapsulation Cytocompatibility DMPG Gelation time Hydrogel Silk Fibroin |
| datacite.titles.title.fl_str_mv | Phospholipid‐induced silk fibroin hydrogels and their potential as cell carriers for tissue regeneration |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Laomeephol, Chavee Guedes, Marta Cristina Alves Moreira Ferreira, Helena Susana Costa Machado Reis, R. L. Kanokpanont, Sorada Damrongsakkul, Siriporn Neves, N. M. |
| dc.date.Accepted.fl_str_mv | 2020-01-01T00:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/62341 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Wiley |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Cell encapsulation Cytocompatibility DMPG Gelation time Hydrogel Silk Fibroin |
| dc.title.fl_str_mv | Phospholipid‐induced silk fibroin hydrogels and their potential as cell carriers for tissue regeneration |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Silk fibroin (SF) hydrogels can be obtained via self‐assembly, but this process takes several days or weeks, being unfeasible to produce cell carrier hydrogels. In this work, a phospholipid, namely, 1,2‐dimyristoyl‐sn‐glycero‐3‐phospho‐(1′‐rac‐glycerol) sodium salt (DMPG), was used to induce and accelerate the gelation process of SF solutions. Due to the amphipathic nature and negative charge of DMPG, electrostatic and hydrophobic interactions between the phospholipids and SF chains will occur, inducing the structural transition of SF chains to the beta sheet and consequently a rapid gel formation is observed (less than 50 min). Moreover, the gelation time can be controlled by varying the lipid concentration. To assess the potential of the hydrogels as cell carriers, several mammalian cell lines, including L929, NIH/3T3, SaOS‐2, and CaSki, were encapsulated into the hydrogel. The silk‐based hydrogels supported the normal growth of fibroblasts, corroborating their cytocompatibility. Interestingly, an inhibition in the growth of cancer‐derived cell lines was observed. Therefore, DMPG‐induced SF hydrogels can be successfully used as a 3D platform for in situ cell encapsulation, opening promising opportunities in biomedical applications, such as in cell therapies and tissue regeneration. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| id | rum_7ddaf448a2077f647a8a6b5def3ff516 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/62341 |
| inst_facet_str | urn:organizationAcronym:repositorium{{{_:::_}}}Universidade do Minho |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/62341 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Laomeephol, Chavee Guedes, Marta Cristina Alves Moreira Ferreira, Helena Susana Costa Machado Reis, R. L. Kanokpanont, Sorada Damrongsakkul, Siriporn Neves, N. M. |
| publishDate | 2020 |
| publisher.none.fl_str_mv | Wiley |
| repo_facet_str | urn:repositoryAcronym:rum{{{_:::_}}}RepositóriUM - Universidade do Minho |
| reponame_str | RepositóriUM - Universidade do Minho |
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| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engWileyporSilk fibroin (SF) hydrogels can be obtained via self‐assembly, but this process takes several days or weeks, being unfeasible to produce cell carrier hydrogels. In this work, a phospholipid, namely, 1,2‐dimyristoyl‐sn‐glycero‐3‐phospho‐(1′‐rac‐glycerol) sodium salt (DMPG), was used to induce and accelerate the gelation process of SF solutions. Due to the amphipathic nature and negative charge of DMPG, electrostatic and hydrophobic interactions between the phospholipids and SF chains will occur, inducing the structural transition of SF chains to the beta sheet and consequently a rapid gel formation is observed (less than 50 min). Moreover, the gelation time can be controlled by varying the lipid concentration. To assess the potential of the hydrogels as cell carriers, several mammalian cell lines, including L929, NIH/3T3, SaOS‐2, and CaSki, were encapsulated into the hydrogel. The silk‐based hydrogels supported the normal growth of fibroblasts, corroborating their cytocompatibility. Interestingly, an inhibition in the growth of cancer‐derived cell lines was observed. Therefore, DMPG‐induced SF hydrogels can be successfully used as a 3D platform for in situ cell encapsulation, opening promising opportunities in biomedical applications, such as in cell therapies and tissue regeneration.application/pdfporPhospholipid‐induced silk fibroin hydrogels and their potential as cell carriers for tissue regenerationLaomeephol, ChaveeGuedes, Marta Cristina Alves MoreiraFerreira, Helena Susana Costa MachadoReis, R. L.Kanokpanont, SoradaDamrongsakkul, SiripornNeves, N. M.HostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONLaomeephol C., Guedes M., Ferreira H., Reis R. L., Kanokpanont S., Damrongsakkul S., Neves N. M. Phospholipid‐induced silk fibroin hydrogels and their potential as cell carriers for tissue regeneration, Journal Of Tissue Engineering And Regenerative Medicine, pp. 1-13, doi:10.1002/term.2982, 2020PMID31671250ISSNIsPartOf1932-6254EISSNIsPartOf1932-7005DOIIsPartOf10.1002/term.298220202020-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/62341http://purl.org/coar/access_right/c_abf2open accessCell encapsulationCytocompatibilityDMPGGelation timeHydrogelSilk Fibroin20377166 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdfhttps://repositorium.uminho.pt/bitstreams/88875d6c-43f6-49fc-b050-38098027d1e2/download |
| spellingShingle | Phospholipid‐induced silk fibroin hydrogels and their potential as cell carriers for tissue regeneration Laomeephol, Chavee Cell encapsulation Cytocompatibility DMPG Gelation time Hydrogel Silk Fibroin |
| status | SINGLETON |
| subject.fl_str_mv | Cell encapsulation Cytocompatibility DMPG Gelation time Hydrogel Silk Fibroin |
| title | Phospholipid‐induced silk fibroin hydrogels and their potential as cell carriers for tissue regeneration |
| title_full | Phospholipid‐induced silk fibroin hydrogels and their potential as cell carriers for tissue regeneration |
| title_fullStr | Phospholipid‐induced silk fibroin hydrogels and their potential as cell carriers for tissue regeneration |
| title_full_unstemmed | Phospholipid‐induced silk fibroin hydrogels and their potential as cell carriers for tissue regeneration |
| title_short | Phospholipid‐induced silk fibroin hydrogels and their potential as cell carriers for tissue regeneration |
| title_sort | Phospholipid‐induced silk fibroin hydrogels and their potential as cell carriers for tissue regeneration |
| topic | Cell encapsulation Cytocompatibility DMPG Gelation time Hydrogel Silk Fibroin |
| topic_facet | Cell encapsulation Cytocompatibility DMPG Gelation time Hydrogel Silk Fibroin |
| url | https://hdl.handle.net/1822/62341 |
| visible | 1 |
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