Publicação
Vaginal sheets with Thymbra capitata essential oil for the treatment of bacterial vaginosis: design, characterization and in vitro evaluation of efficacy and safety
| Resumo: | We aimed to incorporate Thymbra capitata essential oil (TCEO), a potent antimicrobial natural product against bacterial vaginosis (BV)-related bacteria, in a suitable drug delivery system. We used vaginal sheets as dosage form to promote immediate relief of the typical abundant vaginal discharge with unpleasant odour. Excipients were selected to promote the healthy vaginal environment reestablishment and bioadhesion of formulations, while the TCEO acts directly on BV pathogens. We characterized vaginal sheets with TCEO in regard to technological characterization, predictable in vivo performance, in vitro efficacy and safety. Vaginal sheet D.O (acid lactic buffer, gelatine, glycerine, chitosan coated with TCEO 1% w/w) presented a higher buffer capacity and ability to absorb vaginal fluid simulant (VFS) among all vaginal sheets with EO, showing one of the most promising bioadhesive profiles, an excellent flexibility and structure that allow it to be easily rolled for application. Vaginal sheet D.O with 0.32 µL/mL TCEO was able to significantly reduce the bacterial load of all in vitro tested Gardnerella species. Although vaginal sheet D.O presented toxicity at some concentrations, this product was developed for a short time period of treatment, so this toxicity can probably be limited or even reversed when the treatment ends. |
|---|---|
| Autores principais: | Tomás, Mariana |
| Outros Autores: | Sousa, Lúcia Filipa Guimarães Vieira; Oliveira, Ana Sofia; Gomes, Carolina P.; Palmeira-de-Oliveira, Ana; Cavaleiro, Carlos; Salgueiro, Lígia; Cerca, Nuno; Martinez-de-Oliveira, José; Palmeira-de-Oliveira, Rita |
| Assunto: | Vaginal infections Bacterial vaginosis Thymbra capitata essential oil Treatment Gardnerella several species (spp.) Vaginal sheet |
| Ano: | 2023 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1877776501162639360 |
|---|---|
| author | Tomás, Mariana |
| author2 | Sousa, Lúcia Filipa Guimarães Vieira Oliveira, Ana Sofia Gomes, Carolina P. Palmeira-de-Oliveira, Ana Cavaleiro, Carlos Salgueiro, Lígia Cerca, Nuno Martinez-de-Oliveira, José Palmeira-de-Oliveira, Rita |
| author2_role | author author author author author author author author author |
| author_facet | Tomás, Mariana Sousa, Lúcia Filipa Guimarães Vieira Oliveira, Ana Sofia Gomes, Carolina P. Palmeira-de-Oliveira, Ana Cavaleiro, Carlos Salgueiro, Lígia Cerca, Nuno Martinez-de-Oliveira, José Palmeira-de-Oliveira, Rita |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Tomás, Mariana\"},{\"Person.name\":\"Sousa, Lúcia Filipa Guimarães Vieira\"},{\"Person.name\":\"Oliveira, Ana Sofia\"},{\"Person.name\":\"Gomes, Carolina P.\"},{\"Person.name\":\"Palmeira-de-Oliveira, Ana\"},{\"Person.name\":\"Cavaleiro, Carlos\"},{\"Person.name\":\"Salgueiro, Lígia\"},{\"Person.name\":\"Cerca, Nuno\"},{\"Person.name\":\"Martinez-de-Oliveira, José\"},{\"Person.name\":\"Palmeira-de-Oliveira, Rita\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Tomás, Mariana Sousa, Lúcia Filipa Guimarães Vieira Oliveira, Ana Sofia Gomes, Carolina P. Palmeira-de-Oliveira, Ana Cavaleiro, Carlos Salgueiro, Lígia Cerca, Nuno Martinez-de-Oliveira, José Palmeira-de-Oliveira, Rita |
| datacite.date.Accepted.fl_str_mv | 2023-04-02T00:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Vaginal infections Bacterial vaginosis Thymbra capitata essential oil Treatment Gardnerella several species (spp.) Vaginal sheet |
| datacite.titles.title.fl_str_mv | Vaginal sheets with Thymbra capitata essential oil for the treatment of bacterial vaginosis: design, characterization and in vitro evaluation of efficacy and safety |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Tomás, Mariana Sousa, Lúcia Filipa Guimarães Vieira Oliveira, Ana Sofia Gomes, Carolina P. Palmeira-de-Oliveira, Ana Cavaleiro, Carlos Salgueiro, Lígia Cerca, Nuno Martinez-de-Oliveira, José Palmeira-de-Oliveira, Rita |
| dc.date.Accepted.fl_str_mv | 2023-04-02T00:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/83820 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Multidisciplinary Digital Publishing Institute (MDPI) |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.rights.rights.copyright.fl_str_mv | openAccess |
| dc.subject.none.fl_str_mv | Vaginal infections Bacterial vaginosis Thymbra capitata essential oil Treatment Gardnerella several species (spp.) Vaginal sheet |
| dc.title.fl_str_mv | Vaginal sheets with Thymbra capitata essential oil for the treatment of bacterial vaginosis: design, characterization and in vitro evaluation of efficacy and safety |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | We aimed to incorporate Thymbra capitata essential oil (TCEO), a potent antimicrobial natural product against bacterial vaginosis (BV)-related bacteria, in a suitable drug delivery system. We used vaginal sheets as dosage form to promote immediate relief of the typical abundant vaginal discharge with unpleasant odour. Excipients were selected to promote the healthy vaginal environment reestablishment and bioadhesion of formulations, while the TCEO acts directly on BV pathogens. We characterized vaginal sheets with TCEO in regard to technological characterization, predictable in vivo performance, in vitro efficacy and safety. Vaginal sheet D.O (acid lactic buffer, gelatine, glycerine, chitosan coated with TCEO 1% w/w) presented a higher buffer capacity and ability to absorb vaginal fluid simulant (VFS) among all vaginal sheets with EO, showing one of the most promising bioadhesive profiles, an excellent flexibility and structure that allow it to be easily rolled for application. Vaginal sheet D.O with 0.32 µL/mL TCEO was able to significantly reduce the bacterial load of all in vitro tested Gardnerella species. Although vaginal sheet D.O presented toxicity at some concentrations, this product was developed for a short time period of treatment, so this toxicity can probably be limited or even reversed when the treatment ends. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| id | rum_4384edafda8449e2de4a1f3d8f81a2b7 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/83820 |
| inst_facet_str | urn:organizationAcronym:repositorium{{{_:::_}}}Universidade do Minho |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/83820 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Tomás, Mariana Sousa, Lúcia Filipa Guimarães Vieira Oliveira, Ana Sofia Gomes, Carolina P. Palmeira-de-Oliveira, Ana Cavaleiro, Carlos Salgueiro, Lígia Cerca, Nuno Martinez-de-Oliveira, José Palmeira-de-Oliveira, Rita |
| publishDate | 2023 |
| publisher.none.fl_str_mv | Multidisciplinary Digital Publishing Institute (MDPI) |
| repo_facet_str | urn:repositoryAcronym:rum{{{_:::_}}}RepositóriUM - Universidade do Minho |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engMultidisciplinary Digital Publishing Institute (MDPI)porWe aimed to incorporate Thymbra capitata essential oil (TCEO), a potent antimicrobial natural product against bacterial vaginosis (BV)-related bacteria, in a suitable drug delivery system. We used vaginal sheets as dosage form to promote immediate relief of the typical abundant vaginal discharge with unpleasant odour. Excipients were selected to promote the healthy vaginal environment reestablishment and bioadhesion of formulations, while the TCEO acts directly on BV pathogens. We characterized vaginal sheets with TCEO in regard to technological characterization, predictable in vivo performance, in vitro efficacy and safety. Vaginal sheet D.O (acid lactic buffer, gelatine, glycerine, chitosan coated with TCEO 1% w/w) presented a higher buffer capacity and ability to absorb vaginal fluid simulant (VFS) among all vaginal sheets with EO, showing one of the most promising bioadhesive profiles, an excellent flexibility and structure that allow it to be easily rolled for application. Vaginal sheet D.O with 0.32 µL/mL TCEO was able to significantly reduce the bacterial load of all in vitro tested Gardnerella species. Although vaginal sheet D.O presented toxicity at some concentrations, this product was developed for a short time period of treatment, so this toxicity can probably be limited or even reversed when the treatment ends.application/pdfengVaginal sheets with Thymbra capitata essential oil for the treatment of bacterial vaginosis: design, characterization and in vitro evaluation of efficacy and safetyTomás, MarianaSousa, Lúcia Filipa Guimarães VieiraOliveira, Ana SofiaGomes, Carolina P.Palmeira-de-Oliveira, AnaCavaleiro, CarlosSalgueiro, LígiaCerca, NunoMartinez-de-Oliveira, JoséPalmeira-de-Oliveira, RitaHostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONTomás, M., Sousa, L. G. V., Oliveira, A. S., Gomes, C. P., Palmeira-de-Oliveira, A., Cavaleiro, C., Salgueiro, L., Cerca, N., Martinez-de-Oliveira, J., & Palmeira-de-Oliveira, R. (2023). Vaginal Sheets with Thymbra capitata Essential Oil for the Treatment of Bacterial Vaginosis: Design, Characterization and In Vitro Evaluation of Efficacy and Safety. Gels, 9(4), 293. https://doi.org/10.3390/gels9040293ARTICLENUMBER293EISSNIsPartOf2310-2861DOIIsPartOf10.3390/gels90402932023-04-022023-04-02T00:00:00ZHandlehttps://hdl.handle.net/1822/83820http://purl.org/coar/access_right/c_abf2open accessVaginal infectionsBacterial vaginosisThymbra capitata essential oilTreatmentGardnerella several species (spp.)Vaginal sheet1914692 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2023-04-02http://creativecommons.org/licenses/by/4.0/openAccesshttp://purl.org/coar/access_right/c_abf2application/pdfhttps://repositorium.uminho.pt/bitstreams/408dce87-3fe8-42b6-a4e1-f14a86557f8f/download |
| spellingShingle | Vaginal sheets with Thymbra capitata essential oil for the treatment of bacterial vaginosis: design, characterization and in vitro evaluation of efficacy and safety Tomás, Mariana Vaginal infections Bacterial vaginosis Thymbra capitata essential oil Treatment Gardnerella several species (spp.) Vaginal sheet |
| status | SINGLETON |
| subject.fl_str_mv | Vaginal infections Bacterial vaginosis Thymbra capitata essential oil Treatment Gardnerella several species (spp.) Vaginal sheet |
| title | Vaginal sheets with Thymbra capitata essential oil for the treatment of bacterial vaginosis: design, characterization and in vitro evaluation of efficacy and safety |
| title_full | Vaginal sheets with Thymbra capitata essential oil for the treatment of bacterial vaginosis: design, characterization and in vitro evaluation of efficacy and safety |
| title_fullStr | Vaginal sheets with Thymbra capitata essential oil for the treatment of bacterial vaginosis: design, characterization and in vitro evaluation of efficacy and safety |
| title_full_unstemmed | Vaginal sheets with Thymbra capitata essential oil for the treatment of bacterial vaginosis: design, characterization and in vitro evaluation of efficacy and safety |
| title_short | Vaginal sheets with Thymbra capitata essential oil for the treatment of bacterial vaginosis: design, characterization and in vitro evaluation of efficacy and safety |
| title_sort | Vaginal sheets with Thymbra capitata essential oil for the treatment of bacterial vaginosis: design, characterization and in vitro evaluation of efficacy and safety |
| topic | Vaginal infections Bacterial vaginosis Thymbra capitata essential oil Treatment Gardnerella several species (spp.) Vaginal sheet |
| topic_facet | Vaginal infections Bacterial vaginosis Thymbra capitata essential oil Treatment Gardnerella several species (spp.) Vaginal sheet |
| url | https://hdl.handle.net/1822/83820 |
| visible | 1 |
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