Publication
In vivo performance of hierarchical HRP-crosslinked silk fibroin/β-TCP scaffolds for osteochondral tissue regeneration
| Summary: | Background: Osteochondral defects (OCD) can affect the articular cartilage and subchondral bone tissues, which requires superior therapies for the simultaneous and full restoration of such structurally and biologically different tissues. Methods: Tissue engineered OC grafts were prepared using a horseradish peroxidase (HRP) approach to crosslink silk fibroin (HRP-SF) as the articular cartilage-like layer and an underlying HRP-SF/ZnSrTCP subchondral bone-like layer (HRP-SF/dTCP), through salt-leaching/freeze-drying methodologies. In vivo OC regeneration was assessed by implantating the hierarchical scaffolds in rabbit critical size OC defects, during 8 weeks. A comparative analysis was performed using hierarchical OC grafts made of pure β-TCP (HRP-SF/TCP). Results: The hierarchical scaffolds showed good integration into the host tissue and no signs of acute inflammatory reaction, after 8 weeks of implantation. The histological analyses revealed positive collagen type II and glycosaminoglycansâ formation in the articular cartilage-like layer. New bone ingrowthâ s and blood vessels infiltration were detected in the subchondral bone-like layers. Conclusions: The proposed hierarchical scaffolds presented an adequate in vivo response with cartilage tissue regeneration and calcified tissue formation specially promoted by the ionic incorporation into the subchondral bone layer, confirming the hierarchical structures as suitable for OCD regeneration. |
|---|---|
| Main Authors: | Ribeiro, Viviana Pinto |
| Other Authors: | Pina, Sandra Cristina Almeida; Canadas, R. F.; Morais, A.; Vilela, C.; Vieira, Sílvia Cristina Araújo; Cengiz, I. F.; Reis, R. L.; Oliveira, Joaquim M. |
| Subject: | Hierarchical scaffolds Horseradish peroxidase-mediated crosslinking Osteochondral regeneration Rabbit critical defect Silk Fibroin ZnSr-tricalcium phosphate |
| Year: | 2019 |
| Country: | Portugal |
| Document type: | article |
| Access type: | open access |
| Associated institution: | Universidade do Minho |
| Language: | English |
| Origin: | RepositóriUM - Universidade do Minho |
| _version_ | 1877774533517115392 |
|---|---|
| author | Ribeiro, Viviana Pinto |
| author2 | Pina, Sandra Cristina Almeida Canadas, R. F. Morais, A. Vilela, C. Vieira, Sílvia Cristina Araújo Cengiz, I. F. Reis, R. L. Oliveira, Joaquim M. |
| author2_role | author author author author author author author author |
| author_facet | Ribeiro, Viviana Pinto Pina, Sandra Cristina Almeida Canadas, R. F. Morais, A. Vilela, C. Vieira, Sílvia Cristina Araújo Cengiz, I. F. Reis, R. L. Oliveira, Joaquim M. |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Ribeiro, Viviana Pinto\"},{\"Person.name\":\"Pina, Sandra Cristina Almeida\"},{\"Person.name\":\"Canadas, R. F.\"},{\"Person.name\":\"Morais, A.\"},{\"Person.name\":\"Vilela, C.\"},{\"Person.name\":\"Vieira, Sílvia Cristina Araújo\"},{\"Person.name\":\"Cengiz, I. F.\"},{\"Person.name\":\"Reis, R. L.\"},{\"Person.name\":\"Oliveira, Joaquim M.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Ribeiro, Viviana Pinto Pina, Sandra Cristina Almeida Canadas, R. F. Morais, A. Vilela, C. Vieira, Sílvia Cristina Araújo Cengiz, I. F. Reis, R. L. Oliveira, Joaquim M. |
| datacite.date.Accepted.fl_str_mv | 2019-09-01T00:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Hierarchical scaffolds Horseradish peroxidase-mediated crosslinking Osteochondral regeneration Rabbit critical defect Silk Fibroin ZnSr-tricalcium phosphate |
| datacite.titles.title.fl_str_mv | In vivo performance of hierarchical HRP-crosslinked silk fibroin/β-TCP scaffolds for osteochondral tissue regeneration |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Ribeiro, Viviana Pinto Pina, Sandra Cristina Almeida Canadas, R. F. Morais, A. Vilela, C. Vieira, Sílvia Cristina Araújo Cengiz, I. F. Reis, R. L. Oliveira, Joaquim M. |
| dc.date.Accepted.fl_str_mv | 2019-09-01T00:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/61432 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Hapres |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Hierarchical scaffolds Horseradish peroxidase-mediated crosslinking Osteochondral regeneration Rabbit critical defect Silk Fibroin ZnSr-tricalcium phosphate |
| dc.title.fl_str_mv | In vivo performance of hierarchical HRP-crosslinked silk fibroin/β-TCP scaffolds for osteochondral tissue regeneration |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Background: Osteochondral defects (OCD) can affect the articular cartilage and subchondral bone tissues, which requires superior therapies for the simultaneous and full restoration of such structurally and biologically different tissues. Methods: Tissue engineered OC grafts were prepared using a horseradish peroxidase (HRP) approach to crosslink silk fibroin (HRP-SF) as the articular cartilage-like layer and an underlying HRP-SF/ZnSrTCP subchondral bone-like layer (HRP-SF/dTCP), through salt-leaching/freeze-drying methodologies. In vivo OC regeneration was assessed by implantating the hierarchical scaffolds in rabbit critical size OC defects, during 8 weeks. A comparative analysis was performed using hierarchical OC grafts made of pure β-TCP (HRP-SF/TCP). Results: The hierarchical scaffolds showed good integration into the host tissue and no signs of acute inflammatory reaction, after 8 weeks of implantation. The histological analyses revealed positive collagen type II and glycosaminoglycansâ formation in the articular cartilage-like layer. New bone ingrowthâ s and blood vessels infiltration were detected in the subchondral bone-like layers. Conclusions: The proposed hierarchical scaffolds presented an adequate in vivo response with cartilage tissue regeneration and calcified tissue formation specially promoted by the ionic incorporation into the subchondral bone layer, confirming the hierarchical structures as suitable for OCD regeneration. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| id | rum_397e442b1ae84653a098f60dabc46688 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/61432 |
| inst_facet_str | urn:organizationAcronym:repositorium{{{_:::_}}}Universidade do Minho |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/61432 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Ribeiro, Viviana Pinto Pina, Sandra Cristina Almeida Canadas, R. F. Morais, A. Vilela, C. Vieira, Sílvia Cristina Araújo Cengiz, I. F. Reis, R. L. Oliveira, Joaquim M. |
| publishDate | 2019 |
| publisher.none.fl_str_mv | Hapres |
| repo_facet_str | urn:repositoryAcronym:rum{{{_:::_}}}RepositóriUM - Universidade do Minho |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engHapresporBackground: Osteochondral defects (OCD) can affect the articular cartilage and subchondral bone tissues, which requires superior therapies for the simultaneous and full restoration of such structurally and biologically different tissues. Methods: Tissue engineered OC grafts were prepared using a horseradish peroxidase (HRP) approach to crosslink silk fibroin (HRP-SF) as the articular cartilage-like layer and an underlying HRP-SF/ZnSrTCP subchondral bone-like layer (HRP-SF/dTCP), through salt-leaching/freeze-drying methodologies. In vivo OC regeneration was assessed by implantating the hierarchical scaffolds in rabbit critical size OC defects, during 8 weeks. A comparative analysis was performed using hierarchical OC grafts made of pure β-TCP (HRP-SF/TCP). Results: The hierarchical scaffolds showed good integration into the host tissue and no signs of acute inflammatory reaction, after 8 weeks of implantation. The histological analyses revealed positive collagen type II and glycosaminoglycansâ formation in the articular cartilage-like layer. New bone ingrowthâ s and blood vessels infiltration were detected in the subchondral bone-like layers. Conclusions: The proposed hierarchical scaffolds presented an adequate in vivo response with cartilage tissue regeneration and calcified tissue formation specially promoted by the ionic incorporation into the subchondral bone layer, confirming the hierarchical structures as suitable for OCD regeneration.application/pdfporIn vivo performance of hierarchical HRP-crosslinked silk fibroin/β-TCP scaffolds for osteochondral tissue regenerationRibeiro, Viviana PintoPina, Sandra Cristina AlmeidaCanadas, R. F.Morais, A.Vilela, C.Vieira, Sílvia Cristina AraújoCengiz, I. F.Reis, R. L.Oliveira, Joaquim M.HostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONRibeiro V. P., Pina S., Canadas R. F., Morais A., Vilela C., Vieira S., Cengiz I. F., Reis R. L., Oliveira J. M. In Vivo Performance of Hierarchical HRP-Crosslinked Silk Fibroin/β-TCP Scaffolds for Osteochondral Tissue Regeneration, Regenerative Medicine Frontiers, doi:10.20900/rmf20190007, 2019ISSNIsPartOf2633-0806DOIIsPartOf10.20900/rmf201900072019-092019-09-01T00:00:00ZHandlehttps://hdl.handle.net/1822/61432http://purl.org/coar/access_right/c_abf2open accessHierarchical scaffoldsHorseradish peroxidase-mediated crosslinkingOsteochondral regenerationRabbit critical defectSilk FibroinZnSr-tricalcium phosphate19062766 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdfhttps://repositorium.uminho.pt/bitstreams/6d6ca519-f7ef-41bf-ad22-0661ff4068ab/download |
| spellingShingle | In vivo performance of hierarchical HRP-crosslinked silk fibroin/β-TCP scaffolds for osteochondral tissue regeneration Ribeiro, Viviana Pinto Hierarchical scaffolds Horseradish peroxidase-mediated crosslinking Osteochondral regeneration Rabbit critical defect Silk Fibroin ZnSr-tricalcium phosphate |
| status | SINGLETON |
| subject.fl_str_mv | Hierarchical scaffolds Horseradish peroxidase-mediated crosslinking Osteochondral regeneration Rabbit critical defect Silk Fibroin ZnSr-tricalcium phosphate |
| title | In vivo performance of hierarchical HRP-crosslinked silk fibroin/β-TCP scaffolds for osteochondral tissue regeneration |
| title_full | In vivo performance of hierarchical HRP-crosslinked silk fibroin/β-TCP scaffolds for osteochondral tissue regeneration |
| title_fullStr | In vivo performance of hierarchical HRP-crosslinked silk fibroin/β-TCP scaffolds for osteochondral tissue regeneration |
| title_full_unstemmed | In vivo performance of hierarchical HRP-crosslinked silk fibroin/β-TCP scaffolds for osteochondral tissue regeneration |
| title_short | In vivo performance of hierarchical HRP-crosslinked silk fibroin/β-TCP scaffolds for osteochondral tissue regeneration |
| title_sort | In vivo performance of hierarchical HRP-crosslinked silk fibroin/β-TCP scaffolds for osteochondral tissue regeneration |
| topic | Hierarchical scaffolds Horseradish peroxidase-mediated crosslinking Osteochondral regeneration Rabbit critical defect Silk Fibroin ZnSr-tricalcium phosphate |
| topic_facet | Hierarchical scaffolds Horseradish peroxidase-mediated crosslinking Osteochondral regeneration Rabbit critical defect Silk Fibroin ZnSr-tricalcium phosphate |
| url | https://hdl.handle.net/1822/61432 |
| visible | 1 |
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