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Maximization of fructose esters synthesis by response surface methodology

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Summary:Enzymatic synthesis of fructose fatty acide sterwasper formed inorganic solventmedia, using apurified lipase from Candida antartica B immobilizedinacrylicresin. Response sur face methodology with a central composit erotatable design based on five levels was implemented to optimize three experimental operating conditions (temperature, agitation and reaction time). A statistical significant cubic model was established. Temperature andre action time were found to bethe most significant parameters. The optimum operational conditions for maximizing the synthesis of fructos e esters were 57.18C, 100rpm and 37.8h. The model was validated in the identified optimal condition stocheck its adequacy and accuracy, and an experimental esterification percentage of 88.4% ( 0.3%) was obtained. These results showed that animprovement of the enzymatic synthesis of fructose esters was obtained under the optimized conditions
Main Authors:Neta, Nair do Amaral Sampaio
Other Authors:Peres, A. M.; Teixeira, J. A.; Rodrigues, L. R.
Subject:Candida antartica B Fructose esters Response surface methodology
Year:2011
Country:Portugal
Document type:article
Access type:open access
Associated institution:Universidade do Minho
Language:English
Origin:RepositóriUM - Universidade do Minho

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