Publication
3D Bioprinted Highly Elastic Hybrid Constructs for Advanced Fibrocartilaginous Tissue Regeneration
| Summary: | Advanced strategies to bioengineer a fibrocartilaginous tissue to restore the function of the meniscus are necessary. Currently, 3D bioprinting technologies have been employed to fabricate clinically relevant patient-specific complex constructs to address unmet clinical needs. In this study, a highly elastic hybrid construct for fibrocartilaginous regeneration is produced by coprinting a cell-laden gellan gum/fibrinogen (GG/FB) composite bioink together with a silk fibroin methacrylate (Sil-MA) bioink in an interleaved crosshatch pattern. We characterize each bioink formulation by measuring the rheological properties, swelling ratio, and compressive mechanical behavior. For in vitro biological evaluations, porcine primary meniscus cells (pMCs) are isolated and suspended in the GG/FB bioink for the printing process. The results show that the GG/FB bioink provides a proper cellular microenvironment for maintaining the cell viability and proliferation capacity, as well as the maturation of the pMCs in the bioprinted constructs, while the Sil-MA bioink offers excellent biomechanical behavior and structural integrity. More importantly, this bioprinted hybrid system shows the fibrocartilaginous tissue formation without a dimensional change in a mouse subcutaneous implantation model during the 10-week postimplantation. Especially, the alignment of collagen fibers is achieved in the bioprinted hybrid constructs. The results demonstrate this bioprinted mechanically reinforced hybrid construct offers a versatile and promising alternative for the production of advanced fibrocartilaginous tissue. |
|---|---|
| Main Authors: | Costa, João Pedro Bebiano |
| Other Authors: | Park, J.; Jorgensen, A. M.; Silva-Correia, Joana; Reis, R. L.; Oliveira, J. M.; Atala, A.; Yoo, J. J.; Lee, S. J. |
| Subject: | Bioprinting fibrocartilage Hybrid Ciências Médicas::Medicina Básica |
| Year: | 2020 |
| Country: | Portugal |
| Document type: | article |
| Access type: | restricted access |
| Associated institution: | Universidade do Minho |
| Language: | English |
| Origin: | RepositóriUM - Universidade do Minho |
| _version_ | 1877774401139638272 |
|---|---|
| author | Costa, João Pedro Bebiano |
| author2 | Park, J. Jorgensen, A. M. Silva-Correia, Joana Reis, R. L. Oliveira, J. M. Atala, A. Yoo, J. J. Lee, S. J. |
| author2_role | author author author author author author author author |
| author_facet | Costa, João Pedro Bebiano Park, J. Jorgensen, A. M. Silva-Correia, Joana Reis, R. L. Oliveira, J. M. Atala, A. Yoo, J. J. Lee, S. J. |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Costa, João Pedro Bebiano\"},{\"Person.name\":\"Park, J.\"},{\"Person.name\":\"Jorgensen, A. M.\"},{\"Person.name\":\"Silva-Correia, Joana\"},{\"Person.name\":\"Reis, R. L.\"},{\"Person.name\":\"Oliveira, J. M.\"},{\"Person.name\":\"Atala, A.\"},{\"Person.name\":\"Yoo, J. J.\"},{\"Person.name\":\"Lee, S. J.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Costa, João Pedro Bebiano Park, J. Jorgensen, A. M. Silva-Correia, Joana Reis, R. L. Oliveira, J. M. Atala, A. Yoo, J. J. Lee, S. J. |
| datacite.date.Accepted.fl_str_mv | 2020-09-01T00:00:00Z |
| datacite.date.embargoed.fl_str_mv | 10000-01-01T00:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Bioprinting fibrocartilage Hybrid Ciências Médicas::Medicina Básica |
| datacite.titles.title.fl_str_mv | 3D Bioprinted Highly Elastic Hybrid Constructs for Advanced Fibrocartilaginous Tissue Regeneration |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Costa, João Pedro Bebiano Park, J. Jorgensen, A. M. Silva-Correia, Joana Reis, R. L. Oliveira, J. M. Atala, A. Yoo, J. J. Lee, S. J. |
| dc.date.Accepted.fl_str_mv | 2020-09-01T00:00:00Z |
| dc.date.embargoed.fl_str_mv | 10000-01-01T00:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/67158 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | American Chemical Society |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Bioprinting fibrocartilage Hybrid Ciências Médicas::Medicina Básica |
| dc.title.fl_str_mv | 3D Bioprinted Highly Elastic Hybrid Constructs for Advanced Fibrocartilaginous Tissue Regeneration |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Advanced strategies to bioengineer a fibrocartilaginous tissue to restore the function of the meniscus are necessary. Currently, 3D bioprinting technologies have been employed to fabricate clinically relevant patient-specific complex constructs to address unmet clinical needs. In this study, a highly elastic hybrid construct for fibrocartilaginous regeneration is produced by coprinting a cell-laden gellan gum/fibrinogen (GG/FB) composite bioink together with a silk fibroin methacrylate (Sil-MA) bioink in an interleaved crosshatch pattern. We characterize each bioink formulation by measuring the rheological properties, swelling ratio, and compressive mechanical behavior. For in vitro biological evaluations, porcine primary meniscus cells (pMCs) are isolated and suspended in the GG/FB bioink for the printing process. The results show that the GG/FB bioink provides a proper cellular microenvironment for maintaining the cell viability and proliferation capacity, as well as the maturation of the pMCs in the bioprinted constructs, while the Sil-MA bioink offers excellent biomechanical behavior and structural integrity. More importantly, this bioprinted hybrid system shows the fibrocartilaginous tissue formation without a dimensional change in a mouse subcutaneous implantation model during the 10-week postimplantation. Especially, the alignment of collagen fibers is achieved in the bioprinted hybrid constructs. The results demonstrate this bioprinted mechanically reinforced hybrid construct offers a versatile and promising alternative for the production of advanced fibrocartilaginous tissue. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
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| id | rum_326a9f42e62a90d73593ecf147c551ec |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/67158 |
| inst_facet_str | urn:organizationAcronym:repositorium{{{_:::_}}}Universidade do Minho |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/67158 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Costa, João Pedro Bebiano Park, J. Jorgensen, A. M. Silva-Correia, Joana Reis, R. L. Oliveira, J. M. Atala, A. Yoo, J. J. Lee, S. J. |
| publishDate | 2020 |
| publisher.none.fl_str_mv | American Chemical Society |
| repo_facet_str | urn:repositoryAcronym:rum{{{_:::_}}}RepositóriUM - Universidade do Minho |
| reponame_str | RepositóriUM - Universidade do Minho |
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| spelling | engAmerican Chemical SocietyporAdvanced strategies to bioengineer a fibrocartilaginous tissue to restore the function of the meniscus are necessary. Currently, 3D bioprinting technologies have been employed to fabricate clinically relevant patient-specific complex constructs to address unmet clinical needs. In this study, a highly elastic hybrid construct for fibrocartilaginous regeneration is produced by coprinting a cell-laden gellan gum/fibrinogen (GG/FB) composite bioink together with a silk fibroin methacrylate (Sil-MA) bioink in an interleaved crosshatch pattern. We characterize each bioink formulation by measuring the rheological properties, swelling ratio, and compressive mechanical behavior. For in vitro biological evaluations, porcine primary meniscus cells (pMCs) are isolated and suspended in the GG/FB bioink for the printing process. The results show that the GG/FB bioink provides a proper cellular microenvironment for maintaining the cell viability and proliferation capacity, as well as the maturation of the pMCs in the bioprinted constructs, while the Sil-MA bioink offers excellent biomechanical behavior and structural integrity. More importantly, this bioprinted hybrid system shows the fibrocartilaginous tissue formation without a dimensional change in a mouse subcutaneous implantation model during the 10-week postimplantation. Especially, the alignment of collagen fibers is achieved in the bioprinted hybrid constructs. The results demonstrate this bioprinted mechanically reinforced hybrid construct offers a versatile and promising alternative for the production of advanced fibrocartilaginous tissue.application/pdfpor3D Bioprinted Highly Elastic Hybrid Constructs for Advanced Fibrocartilaginous Tissue RegenerationCosta, João Pedro BebianoPark, J.Jorgensen, A. M.Silva-Correia, JoanaReis, R. L.Oliveira, J. M.Atala, A.Yoo, J. J.Lee, S. J.HostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONCosta J. B., Park J., Jorgensen A. M., Silva-Correia J., Reis R. L., Oliveira J. M., Atala A., Yoo J. J., Lee S. J. 3D Bioprinted Highly Elastic Hybrid Constructs for Advanced Fibrocartilaginous Tissue Regeneration, Chemistry Of Materials, doi:10.1021/acs.chemmater.0c03556, 2020ISSNIsPartOf0897-4756EISSNIsPartOf1520-5002DOIIsPartOf10.1021/acs.chemmater.0c035562020-092020-09-01T00:00:00Z10000-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/67158http://purl.org/coar/access_right/c_16ecrestricted accessBioprintingfibrocartilageHybridhttp://www.oecd.org/science/inno/38235147.pdfFields of Science and Technology (FOS)Ciências Médicas::Medicina Básica11576809 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_f1cfapplication/pdfhttps://repositorium.uminho.pt/bitstreams/1f6ab1bb-b2e8-4ee8-8569-f8824fc1865b/download |
| spellingShingle | 3D Bioprinted Highly Elastic Hybrid Constructs for Advanced Fibrocartilaginous Tissue Regeneration Costa, João Pedro Bebiano Bioprinting fibrocartilage Hybrid Ciências Médicas::Medicina Básica |
| status | SINGLETON |
| subject.fl_str_mv | Bioprinting fibrocartilage Hybrid |
| subject.other.fl_str_mv | Ciências Médicas::Medicina Básica |
| title | 3D Bioprinted Highly Elastic Hybrid Constructs for Advanced Fibrocartilaginous Tissue Regeneration |
| title_full | 3D Bioprinted Highly Elastic Hybrid Constructs for Advanced Fibrocartilaginous Tissue Regeneration |
| title_fullStr | 3D Bioprinted Highly Elastic Hybrid Constructs for Advanced Fibrocartilaginous Tissue Regeneration |
| title_full_unstemmed | 3D Bioprinted Highly Elastic Hybrid Constructs for Advanced Fibrocartilaginous Tissue Regeneration |
| title_short | 3D Bioprinted Highly Elastic Hybrid Constructs for Advanced Fibrocartilaginous Tissue Regeneration |
| title_sort | 3D Bioprinted Highly Elastic Hybrid Constructs for Advanced Fibrocartilaginous Tissue Regeneration |
| topic | Bioprinting fibrocartilage Hybrid Ciências Médicas::Medicina Básica |
| topic_facet | Bioprinting fibrocartilage Hybrid Ciências Médicas::Medicina Básica |
| url | https://hdl.handle.net/1822/67158 |
| visible | 1 |
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