Publicação
Contact lenses incorporating methotrexate-loaded chitosan/hyaluronic acid nanoparticles
| Resumo: | Nanoparticle-laden contact lenses (CLs) represent a promising strategy for ocular drug delivery. Nanocarriers built from hyaluronic acid and chitosan offer a biomimetic alternative to synthetic polymers, by combining the lubricating property of hyaluronic acid with the mucoadhesive property of chitosan. However, achieving sustained drug release without compromising lens properties remains challenging, and the influence of lens material and replacement modality is unclear. This study evaluated methotrexate (MTX)-loaded chitosan/hyaluronic acid (CS/HA) nanoparticles incorporated into silicone hydrogel CLs with different replacement modalities, assessing their effect on drug release kinetics. Daily replacement lenses (Senofilcon A and Delefilcon A) released 26 ± 4% and 33 ± 5% of MTX after 24 h, respectively. The monthly lens Lehfilcon A showed slower diffusion-controlled release, with only 15 ± 2% released at 24 h and a 64 ± 3% cumulative release after 31 days (p < 0.01). Nanoparticle incorporation improved drug retention and reduced initial drug loss compared with lenses loaded with only MTX. Monthly lenses demonstrated sustained delivery potential, supporting prolonged ocular therapy, while daily lenses may be better suited for short-term treatment. These findings reinforce the value of bioinspired nanocarriers, mimicking natural retention mechanisms of the ocular surface, for next-generation therapeutic CLs. |
|---|---|
| Autores principais: | Vale, Sofia |
| Outros Autores: | Senra, Sara F. M.; Veloso, Sérgio Rafael Silva; Castanheira, Elisabete M. S.; Lira, Madalena |
| Assunto: | therapeutic contact lenses drug delivery methotrexate nanoparticles chitosan hyaluronic acid biomimetic materials Ciências Naturais::Ciências Físicas Ciências Médicas::Ciências da Saúde Engenharia e Tecnologia::Nanotecnologia Saúde de qualidade Produção e consumo sustentáveis |
| Ano: | 2026 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1877776874275340288 |
|---|---|
| author | Vale, Sofia |
| author2 | Senra, Sara F. M. Veloso, Sérgio Rafael Silva Castanheira, Elisabete M. S. Lira, Madalena |
| author2_role | author author author author |
| author_facet | Vale, Sofia Senra, Sara F. M. Veloso, Sérgio Rafael Silva Castanheira, Elisabete M. S. Lira, Madalena |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Vale, Sofia\"},{\"Person.name\":\"Senra, Sara F. M.\"},{\"Person.name\":\"Veloso, Sérgio Rafael Silva\"},{\"Person.name\":\"Castanheira, Elisabete M. S.\"},{\"Person.name\":\"Lira, Madalena\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Vale, Sofia Senra, Sara F. M. Veloso, Sérgio Rafael Silva Castanheira, Elisabete M. S. Lira, Madalena |
| datacite.date.Accepted.fl_str_mv | 2026-08-01T00:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | therapeutic contact lenses drug delivery methotrexate nanoparticles chitosan hyaluronic acid biomimetic materials Ciências Naturais::Ciências Físicas Ciências Médicas::Ciências da Saúde Engenharia e Tecnologia::Nanotecnologia Saúde de qualidade Produção e consumo sustentáveis |
| datacite.titles.title.fl_str_mv | Contact lenses incorporating methotrexate-loaded chitosan/hyaluronic acid nanoparticles |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Vale, Sofia Senra, Sara F. M. Veloso, Sérgio Rafael Silva Castanheira, Elisabete M. S. Lira, Madalena |
| dc.date.Accepted.fl_str_mv | 2026-08-01T00:00:00Z |
| dc.format.none.fl_str_mv | application/pdf application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/103254 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Multidisciplinary Digital Publishing Institute (MDPI) |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.rights.rights.copyright.fl_str_mv | openAccess |
| dc.subject.none.fl_str_mv | therapeutic contact lenses drug delivery methotrexate nanoparticles chitosan hyaluronic acid biomimetic materials Ciências Naturais::Ciências Físicas Ciências Médicas::Ciências da Saúde Engenharia e Tecnologia::Nanotecnologia Saúde de qualidade Produção e consumo sustentáveis |
| dc.title.fl_str_mv | Contact lenses incorporating methotrexate-loaded chitosan/hyaluronic acid nanoparticles |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Nanoparticle-laden contact lenses (CLs) represent a promising strategy for ocular drug delivery. Nanocarriers built from hyaluronic acid and chitosan offer a biomimetic alternative to synthetic polymers, by combining the lubricating property of hyaluronic acid with the mucoadhesive property of chitosan. However, achieving sustained drug release without compromising lens properties remains challenging, and the influence of lens material and replacement modality is unclear. This study evaluated methotrexate (MTX)-loaded chitosan/hyaluronic acid (CS/HA) nanoparticles incorporated into silicone hydrogel CLs with different replacement modalities, assessing their effect on drug release kinetics. Daily replacement lenses (Senofilcon A and Delefilcon A) released 26 ± 4% and 33 ± 5% of MTX after 24 h, respectively. The monthly lens Lehfilcon A showed slower diffusion-controlled release, with only 15 ± 2% released at 24 h and a 64 ± 3% cumulative release after 31 days (p < 0.01). Nanoparticle incorporation improved drug retention and reduced initial drug loss compared with lenses loaded with only MTX. Monthly lenses demonstrated sustained delivery potential, supporting prolonged ocular therapy, while daily lenses may be better suited for short-term treatment. These findings reinforce the value of bioinspired nanocarriers, mimicking natural retention mechanisms of the ocular surface, for next-generation therapeutic CLs. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| funder_facet_str_mv | FCT{{{_:::_}}}Fundação para a Ciência e a Tecnologia, I.P. |
| funding.funder.alternateName_str_mv | FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia, I.P. |
| funding.identifier_str_mv | UID/04650/2025 |
| funding.name_str_mv | Avaliação UID 2023/2024 |
| funding_str_mv | UID/04650/2025 https://hdl.handle.net/1822/102883 |
| id | rum_15d98ea4cd107ca4322ac7fa67002fe0 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/103254 |
| inst_facet_str | urn:organizationAcronym:repositorium{{{_:::_}}}Universidade do Minho |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/103254 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Vale, Sofia Senra, Sara F. M. Veloso, Sérgio Rafael Silva Castanheira, Elisabete M. S. Lira, Madalena |
| publishDate | 2026 |
| publisher.none.fl_str_mv | Multidisciplinary Digital Publishing Institute (MDPI) |
| repo_facet_str | urn:repositoryAcronym:rum{{{_:::_}}}RepositóriUM - Universidade do Minho |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engMultidisciplinary Digital Publishing Institute (MDPI)engNanoparticle-laden contact lenses (CLs) represent a promising strategy for ocular drug delivery. Nanocarriers built from hyaluronic acid and chitosan offer a biomimetic alternative to synthetic polymers, by combining the lubricating property of hyaluronic acid with the mucoadhesive property of chitosan. However, achieving sustained drug release without compromising lens properties remains challenging, and the influence of lens material and replacement modality is unclear. This study evaluated methotrexate (MTX)-loaded chitosan/hyaluronic acid (CS/HA) nanoparticles incorporated into silicone hydrogel CLs with different replacement modalities, assessing their effect on drug release kinetics. Daily replacement lenses (Senofilcon A and Delefilcon A) released 26 ± 4% and 33 ± 5% of MTX after 24 h, respectively. The monthly lens Lehfilcon A showed slower diffusion-controlled release, with only 15 ± 2% released at 24 h and a 64 ± 3% cumulative release after 31 days (p < 0.01). Nanoparticle incorporation improved drug retention and reduced initial drug loss compared with lenses loaded with only MTX. Monthly lenses demonstrated sustained delivery potential, supporting prolonged ocular therapy, while daily lenses may be better suited for short-term treatment. These findings reinforce the value of bioinspired nanocarriers, mimicking natural retention mechanisms of the ocular surface, for next-generation therapeutic CLs.application/pdfapplication/pdfengContact lenses incorporating methotrexate-loaded chitosan/hyaluronic acid nanoparticlesVale, SofiaSenra, Sara F. M.Veloso, Sérgio Rafael SilvaCastanheira, Elisabete M. S.Lira, MadalenaHostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONVale, S., F. M. Senra, S., Veloso, S. R. S., Castanheira, E. M. S., & Lira, M. (2026). Contact Lenses Incorporating Methotrexate-Loaded Chitosan/Hyaluronic Acid Nanoparticles. Biomimetics, 11(8), 532. https://doi.org/10.3390/biomimetics11080532ARTICLENUMBER532ISSNIsPartOf2313-7673DOIIsPartOf10.3390/biomimetics11080532URLHasVersionhttps://www.mdpi.com/2313-7673/11/8/5322026-08-012026-08-01T00:00:00ZHandlehttps://hdl.handle.net/1822/103254http://purl.org/coar/access_right/c_abf2open accesstherapeutic contact lensesdrug deliverymethotrexatenanoparticleschitosanhyaluronic acidbiomimetic materialshttp://www.oecd.org/science/inno/38235147.pdfFields of Science and Technology (FOS)Ciências Naturais::Ciências Físicashttp://www.oecd.org/science/inno/38235147.pdfFields of Science and Technology (FOS)Ciências Médicas::Ciências da Saúdehttp://www.oecd.org/science/inno/38235147.pdfFields of Science and Technology (FOS)Engenharia e Tecnologia::Nanotecnologiahttps://sdgs.un.org/goalsSustainable Development Goals (SDG)Saúde de qualidadehttps://sdgs.un.org/goalsSustainable Development Goals (SDG)Produção e consumo sustentáveis1883863 bytes222932 bytesFundação para a Ciência e a Tecnologia, I.P.Physics Center of Minho and Porto Universities [UID/04650/2025]Avaliação UID 2023/2024https://hdl.handle.net/1822/102883UID/04650/2025Crossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal article2026-08-01http://creativecommons.org/licenses/by/4.0/openAccesshttp://purl.org/coar/access_right/c_abf2application/pdfhttps://repositorium.uminho.pt/bitstreams/b827aa9a-a58c-44e0-8e6b-f714ac591355/downloadhttp://purl.org/coar/access_right/c_abf2application/pdfhttps://repositorium.uminho.pt/bitstreams/0efdd351-a6b9-4c9c-81a7-781024d628c1/download118 |
| spellingShingle | Contact lenses incorporating methotrexate-loaded chitosan/hyaluronic acid nanoparticles Vale, Sofia therapeutic contact lenses drug delivery methotrexate nanoparticles chitosan hyaluronic acid biomimetic materials Ciências Naturais::Ciências Físicas Ciências Médicas::Ciências da Saúde Engenharia e Tecnologia::Nanotecnologia Saúde de qualidade Produção e consumo sustentáveis |
| status | SINGLETON |
| subject.fl_str_mv | therapeutic contact lenses drug delivery methotrexate nanoparticles chitosan hyaluronic acid biomimetic materials |
| subject.other.fl_str_mv | Ciências Naturais::Ciências Físicas Ciências Médicas::Ciências da Saúde Engenharia e Tecnologia::Nanotecnologia Saúde de qualidade Produção e consumo sustentáveis |
| title | Contact lenses incorporating methotrexate-loaded chitosan/hyaluronic acid nanoparticles |
| title_full | Contact lenses incorporating methotrexate-loaded chitosan/hyaluronic acid nanoparticles |
| title_fullStr | Contact lenses incorporating methotrexate-loaded chitosan/hyaluronic acid nanoparticles |
| title_full_unstemmed | Contact lenses incorporating methotrexate-loaded chitosan/hyaluronic acid nanoparticles |
| title_short | Contact lenses incorporating methotrexate-loaded chitosan/hyaluronic acid nanoparticles |
| title_sort | Contact lenses incorporating methotrexate-loaded chitosan/hyaluronic acid nanoparticles |
| topic | therapeutic contact lenses drug delivery methotrexate nanoparticles chitosan hyaluronic acid biomimetic materials Ciências Naturais::Ciências Físicas Ciências Médicas::Ciências da Saúde Engenharia e Tecnologia::Nanotecnologia Saúde de qualidade Produção e consumo sustentáveis |
| topic_facet | therapeutic contact lenses drug delivery methotrexate nanoparticles chitosan hyaluronic acid biomimetic materials Ciências Naturais::Ciências Físicas Ciências Médicas::Ciências da Saúde Engenharia e Tecnologia::Nanotecnologia Saúde de qualidade Produção e consumo sustentáveis |
| url | https://hdl.handle.net/1822/103254 |
| visible | 1 |