Publicação
Characterization of plasmonic effects in AuNP+rGO composite as a sensing layer for a low-cost lab-on-chip biosensor
| Resumo: | This work deals with the production of a low-cost disposable biosensor for point of care applications. The proposed sensor is a plasmonic structure based on the Localized Surface Plasmon Resonance (LSPR) interaction of metal nanoparticles (MNPs), embedded into a matrix of reduced Graphene Oxide (rGO). After proper functionalization with selective antibodies (Ab), the efficiency of light extinction is controlled by slight changes of the refractive coefficient induced by the concentration of biomarkers trapped by the antibodies on the sensor surface. This work reports a study about the applicability of rGO as a support for gold nanoparticles (AuNPs) for preparing the functionalized LSPR sensing layer. AuNPs are prepared with an economic and eco-friendly method using phytochemicals present in tea extract at room temperature, while a modified Hummer’s method is used to synthesize rGO. The resulting AuNPs-rGO composites are studied in terms of UV-VIS spectroscopy spectral light transmission and plasmonic resonance. The overall analysis is supported by simulation results, obtained by Mie analysis, about the LSPR effect in AuNPs-rGO and its dependence on the biomarker concentration. |
|---|---|
| Autores principais: | Fantoni, Alessandro |
| Outros Autores: | Stojkovic, Vladan; Fernandes, Miguel; Louro, Paula; Vieira, Manuela; Alegria, Elisabete; Da Costa Ribeiro, Ana Paula; Carvalho, Ana; Almeida, M. Gabriela |
| Assunto: | Plasmonic effects AuNP+rGO Lab-on-chip Biosensor |
| Ano: | 2019 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade Politécnica de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Científico da Universidade Politécnica de Lisboa |
| _version_ | 1880398507773263872 |
|---|---|
| author | Fantoni, Alessandro |
| author2 | Stojkovic, Vladan Fernandes, Miguel Louro, Paula Vieira, Manuela Alegria, Elisabete Da Costa Ribeiro, Ana Paula Carvalho, Ana Almeida, M. Gabriela |
| author2_role | author author author author author author author author |
| author_facet | Fantoni, Alessandro Stojkovic, Vladan Fernandes, Miguel Louro, Paula Vieira, Manuela Alegria, Elisabete Da Costa Ribeiro, Ana Paula Carvalho, Ana Almeida, M. Gabriela |
| author_role | author |
| contributor_name_str_mv | RCIPL |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Fantoni, Alessandro\",\"Person.identifier.cienciaID\":\"241E-E87C-552F\",\"Person.identifier.orcid\":\"0000-0002-9938-0351\",\"Person.identifier.researcherID\":\"K-1105-2016\",\"Person.identifier.scopusAuthorID\":\"7006535604\"},{\"Person.name\":\"Stojkovic, Vladan\"},{\"Person.name\":\"Fernandes, Miguel\",\"Person.identifier.cienciaID\":\"DD1B-859F-EB0B\",\"Person.identifier.orcid\":\"0000-0002-0765-474X\",\"Person.identifier.scopusAuthorID\":\"24450183800\"},{\"Person.name\":\"Louro, Paula\",\"Person.identifier.cienciaID\":\"E511-8C08-E606\",\"Person.identifier.orcid\":\"0000-0002-4167-2052\",\"Person.identifier.researcherID\":\"U-8346-2017\",\"Person.identifier.scopusAuthorID\":\"8845716400\"},{\"Person.name\":\"Vieira, Manuela\",\"Person.identifier.cienciaID\":\"9516-E25E-BB8E\",\"Person.identifier.orcid\":\"0000-0002-1150-9895\",\"Person.identifier.researcherID\":\"V-7860-2017\",\"Person.identifier.scopusAuthorID\":\"7202140173\",\"Person.identifier.scopusAuthorID\":\"57202593765\"},{\"Person.name\":\"Alegria, Elisabete\",\"Person.identifier.cienciaID\":\"8C1F-851A-9F1C\",\"Person.identifier.orcid\":\"0000-0003-4060-1057\",\"Person.identifier.researcherID\":\"E-9945-2012\",\"Person.identifier.scopusAuthorID\":\"8315848300\"},{\"Person.name\":\"Da Costa Ribeiro, Ana Paula\",\"Person.identifier.cienciaID\":\"E115-5C31-30F4\",\"Person.identifier.orcid\":\"0000-0003-4323-4328\",\"Person.identifier.researcherID\":\"F-8341-2014\",\"Person.identifier.scopusAuthorID\":\"22235818800\"},{\"Person.name\":\"Carvalho, Ana\"},{\"Person.name\":\"Almeida, M. Gabriela\",\"Person.identifier.cienciaID\":\"DF16-FBB4-3564\",\"Person.identifier.orcid\":\"0000-0002-4508-7379\",\"Person.identifier.researcherID\":\"D-8265-2013\",\"Person.identifier.scopusAuthorID\":\"15768609100\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RCIPL |
| datacite.creators.creator.creatorName.fl_str_mv | Fantoni, Alessandro Stojkovic, Vladan Fernandes, Miguel Louro, Paula Vieira, Manuela Alegria, Elisabete Da Costa Ribeiro, Ana Paula Carvalho, Ana Almeida, M. Gabriela |
| datacite.date.Accepted.fl_str_mv | 2019-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2019-12-12T11:22:06Z |
| datacite.date.embargoed.fl_str_mv | 2019-12-12T11:22:06Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Plasmonic effects AuNP+rGO Lab-on-chip Biosensor |
| datacite.titles.title.fl_str_mv | Characterization of plasmonic effects in AuNP+rGO composite as a sensing layer for a low-cost lab-on-chip biosensor |
| dc.contributor.none.fl_str_mv | RCIPL |
| dc.creator.none.fl_str_mv | Fantoni, Alessandro Stojkovic, Vladan Fernandes, Miguel Louro, Paula Vieira, Manuela Alegria, Elisabete Da Costa Ribeiro, Ana Paula Carvalho, Ana Almeida, M. Gabriela |
| dc.date.Accepted.fl_str_mv | 2019-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2019-12-12T11:22:06Z |
| dc.date.embargoed.fl_str_mv | 2019-12-12T11:22:06Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.21/10840 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Spring |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Plasmonic effects AuNP+rGO Lab-on-chip Biosensor |
| dc.title.fl_str_mv | Characterization of plasmonic effects in AuNP+rGO composite as a sensing layer for a low-cost lab-on-chip biosensor |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | This work deals with the production of a low-cost disposable biosensor for point of care applications. The proposed sensor is a plasmonic structure based on the Localized Surface Plasmon Resonance (LSPR) interaction of metal nanoparticles (MNPs), embedded into a matrix of reduced Graphene Oxide (rGO). After proper functionalization with selective antibodies (Ab), the efficiency of light extinction is controlled by slight changes of the refractive coefficient induced by the concentration of biomarkers trapped by the antibodies on the sensor surface. This work reports a study about the applicability of rGO as a support for gold nanoparticles (AuNPs) for preparing the functionalized LSPR sensing layer. AuNPs are prepared with an economic and eco-friendly method using phytochemicals present in tea extract at room temperature, while a modified Hummer’s method is used to synthesize rGO. The resulting AuNPs-rGO composites are studied in terms of UV-VIS spectroscopy spectral light transmission and plasmonic resonance. The overall analysis is supported by simulation results, obtained by Mie analysis, about the LSPR effect in AuNPs-rGO and its dependence on the biomarker concentration. |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10400.21/10840 |
| inst_facet_str | urn:organizationAcronym:ipl{{{_:::_}}}Universidade Politécnica de Lisboa |
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| institution | Universidade Politécnica de Lisboa |
| instname_str | Universidade Politécnica de Lisboa |
| language | eng |
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| person_str_mv | Fantoni, Alessandro Fantoni, Alessandro https://www.ciencia-id.pt/241E-E87C-552F 241E-E87C-552F http://orcid.org/0000-0002-9938-0351 0000-0002-9938-0351 Stojkovic, Vladan Fernandes, Miguel Fernandes, Miguel https://www.ciencia-id.pt/DD1B-859F-EB0B DD1B-859F-EB0B http://orcid.org/0000-0002-0765-474X 0000-0002-0765-474X Louro, Paula Louro, Paula https://www.ciencia-id.pt/E511-8C08-E606 E511-8C08-E606 http://orcid.org/0000-0002-4167-2052 0000-0002-4167-2052 Vieira, Manuela Vieira, Manuela https://www.ciencia-id.pt/9516-E25E-BB8E 9516-E25E-BB8E http://orcid.org/0000-0002-1150-9895 0000-0002-1150-9895 Alegria, Elisabete Alegria, Elisabete https://www.ciencia-id.pt/8C1F-851A-9F1C 8C1F-851A-9F1C http://orcid.org/0000-0003-4060-1057 0000-0003-4060-1057 Da Costa Ribeiro, Ana Paula Da Costa Ribeiro, Ana Paula https://www.ciencia-id.pt/E115-5C31-30F4 E115-5C31-30F4 http://orcid.org/0000-0003-4323-4328 0000-0003-4323-4328 Carvalho, Ana Almeida, M. Gabriela Almeida, M. Gabriela https://www.ciencia-id.pt/DF16-FBB4-3564 DF16-FBB4-3564 http://orcid.org/0000-0002-4508-7379 0000-0002-4508-7379 |
| publishDate | 2019 |
| publisher.none.fl_str_mv | Spring |
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| spelling | engSpringpt_PTThis work deals with the production of a low-cost disposable biosensor for point of care applications. The proposed sensor is a plasmonic structure based on the Localized Surface Plasmon Resonance (LSPR) interaction of metal nanoparticles (MNPs), embedded into a matrix of reduced Graphene Oxide (rGO). After proper functionalization with selective antibodies (Ab), the efficiency of light extinction is controlled by slight changes of the refractive coefficient induced by the concentration of biomarkers trapped by the antibodies on the sensor surface. This work reports a study about the applicability of rGO as a support for gold nanoparticles (AuNPs) for preparing the functionalized LSPR sensing layer. AuNPs are prepared with an economic and eco-friendly method using phytochemicals present in tea extract at room temperature, while a modified Hummer’s method is used to synthesize rGO. The resulting AuNPs-rGO composites are studied in terms of UV-VIS spectroscopy spectral light transmission and plasmonic resonance. The overall analysis is supported by simulation results, obtained by Mie analysis, about the LSPR effect in AuNPs-rGO and its dependence on the biomarker concentration.application/pdfpt_PTCharacterization of plasmonic effects in AuNP+rGO composite as a sensing layer for a low-cost lab-on-chip biosensorPersonalFantoni, AlessandroDSpacehttp://dspace.org/items/9f35eb1e-83e4-4342-a86d-265604301499DSpacehttp://dspace.org/items/9f35eb1e-83e4-4342-a86d-265604301499FantoniAlessandroCiência IDhttps://www.ciencia-id.pt241E-E87C-552FORCIDhttp://orcid.org0000-0002-9938-0351Researcher IDhttps://www.researcherid.comK-1105-2016Scopus Author IDhttps://www.scopus.com7006535604Stojkovic, VladanPersonalFernandes, MiguelDSpacehttp://dspace.org/items/6a72ab08-83fa-4225-be0e-b827f206a82eDSpacehttp://dspace.org/items/6a72ab08-83fa-4225-be0e-b827f206a82eFernandesMiguelCiência IDhttps://www.ciencia-id.ptDD1B-859F-EB0BORCIDhttp://orcid.org0000-0002-0765-474XScopus Author IDhttps://www.scopus.com24450183800PersonalLouro, PaulaDSpacehttp://dspace.org/items/730dc3dd-73eb-428d-b3a6-430085e33585DSpacehttp://dspace.org/items/730dc3dd-73eb-428d-b3a6-430085e33585LouroPaulaCiência IDhttps://www.ciencia-id.ptE511-8C08-E606ORCIDhttp://orcid.org0000-0002-4167-2052Researcher IDhttps://www.researcherid.comU-8346-2017Scopus Author IDhttps://www.scopus.com8845716400PersonalVieira, ManuelaDSpacehttp://dspace.org/items/b77c6785-9cfb-4cd4-90b5-c2b816bd7d11DSpacehttp://dspace.org/items/b77c6785-9cfb-4cd4-90b5-c2b816bd7d11VieiraManuelaCiência IDhttps://www.ciencia-id.pt9516-E25E-BB8EORCIDhttp://orcid.org0000-0002-1150-9895Researcher IDhttps://www.researcherid.comV-7860-2017Scopus Author IDhttps://www.scopus.com7202140173Scopus Author IDhttps://www.scopus.com57202593765PersonalAlegria, ElisabeteDSpacehttp://dspace.org/items/6d781c38-c45e-4159-b795-8c8675606160DSpacehttp://dspace.org/items/6d781c38-c45e-4159-b795-8c8675606160AlegriaElisabeteCiência IDhttps://www.ciencia-id.pt8C1F-851A-9F1CORCIDhttp://orcid.org0000-0003-4060-1057Researcher IDhttps://www.researcherid.comE-9945-2012Scopus Author IDhttps://www.scopus.com8315848300PersonalDa Costa Ribeiro, Ana PaulaDSpacehttp://dspace.org/items/ebe0b25e-d8b0-4c6a-ab27-91f199d36347DSpacehttp://dspace.org/items/ebe0b25e-d8b0-4c6a-ab27-91f199d36347da Costa RibeiroAna PaulaCiência IDhttps://www.ciencia-id.ptE115-5C31-30F4ORCIDhttp://orcid.org0000-0003-4323-4328Researcher IDhttps://www.researcherid.comF-8341-2014Scopus Author IDhttps://www.scopus.com22235818800Carvalho, AnaPersonalAlmeida, M. GabrielaDSpacehttp://dspace.org/items/d21924d0-4d10-4a49-9aff-3e6f83a0e2b1DSpacehttp://dspace.org/items/d21924d0-4d10-4a49-9aff-3e6f83a0e2b1AlmeidaGabrielaCiência IDhttps://www.ciencia-id.ptDF16-FBB4-3564ORCIDhttp://orcid.org0000-0002-4508-7379Researcher IDhttps://www.researcherid.comD-8265-2013Scopus Author IDhttps://www.scopus.com15768609100HostingInstitutionOrganizationalRCIPLe-mailmailto:rcaap@sp.ipl.ptrcaap@sp.ipl.ptISSNIsPartOf1559-94502019-12-12T11:22:06Z20192019-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.21/10840http://purl.org/coar/access_right/c_16ecrestricted accessPlasmonic effectsAuNP+rGOLab-on-chipBiosensor856173 bytesother research producthttp://purl.org/coar/resource_type/c_c94fconference objecthttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorio.ipl.pt/bitstreams/9613eb5c-1d6c-438c-b15a-a5dc1d312d88/download2019 PhotonIcs & Electromagnetics Research Symposium4180418617–20 June 2019 - Rome, Italy |
| spellingShingle | Characterization of plasmonic effects in AuNP+rGO composite as a sensing layer for a low-cost lab-on-chip biosensor Fantoni, Alessandro Plasmonic effects AuNP+rGO Lab-on-chip Biosensor |
| status | SINGLETON |
| subject.fl_str_mv | Plasmonic effects AuNP+rGO Lab-on-chip Biosensor |
| title | Characterization of plasmonic effects in AuNP+rGO composite as a sensing layer for a low-cost lab-on-chip biosensor |
| title_full | Characterization of plasmonic effects in AuNP+rGO composite as a sensing layer for a low-cost lab-on-chip biosensor |
| title_fullStr | Characterization of plasmonic effects in AuNP+rGO composite as a sensing layer for a low-cost lab-on-chip biosensor |
| title_full_unstemmed | Characterization of plasmonic effects in AuNP+rGO composite as a sensing layer for a low-cost lab-on-chip biosensor |
| title_short | Characterization of plasmonic effects in AuNP+rGO composite as a sensing layer for a low-cost lab-on-chip biosensor |
| title_sort | Characterization of plasmonic effects in AuNP+rGO composite as a sensing layer for a low-cost lab-on-chip biosensor |
| topic | Plasmonic effects AuNP+rGO Lab-on-chip Biosensor |
| topic_facet | Plasmonic effects AuNP+rGO Lab-on-chip Biosensor |
| url | http://hdl.handle.net/10400.21/10840 |
| visible | 1 |
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