Publicação
Use of a-SiC:H semiconductor-based transducer for glucose sensing through FRET analysis
| Resumo: | Glucose sensing is an issue with great interest in medical and biological applications. One possible approach to glucose detection takes advantage of measuring changes in fluorescence resonance energy transfer (FRET) between a fluorescent donor and an acceptor within a protein which undergoes glucose-induced changes in conformation. This demands the detection of fluorescent signals in the visible spectrum. In this paper we analyzed the emission spectrum obtained from fluorescent labels attached to a protein which changes its conformation in the presence of glucose using a commercial spectrofluorometer. Different glucose nanosensors were used to measure the output spectra with fluorescent signals located at the cyan and yellow bands of the spectrum. A new device is presented based on multilayered a-SiC:H heterostructures to detect identical transient visible signals. The transducer consists of a p-i'(a-SiC:H)-n/p-i(a-Si:H)-n heterostructure optimized for the detection of the fluorescence resonance energy transfer between fluorophores with excitation in the violet (400 nm) and emissions in the cyan (470 nm) and yellow (588 nm) range of the spectrum. Results show that the device photocurrent signal measured under reverse bias and using appropriate steady state optical bias, allows the separate detection of the cyan and yellow fluorescence signals presented. |
|---|---|
| Autores principais: | Louro, Paula |
| Outros Autores: | Vaz da Silva, V; Vieira, Manuel Augusto; Karmali, Amin; Vieira, Manuela |
| Assunto: | Glucose sensing FRET Nanosensors Fluorescence proteins a-SiC:H photodiodes Multilayered heterostructures Optical sensors |
| Ano: | 2013 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Politécnica de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Científico da Universidade Politécnica de Lisboa |
| _version_ | 1880398382709604352 |
|---|---|
| author | Louro, Paula |
| author2 | Vaz da Silva, V Vieira, Manuel Augusto Karmali, Amin Vieira, Manuela |
| author2_role | author author author author |
| author_facet | Louro, Paula Vaz da Silva, V Vieira, Manuel Augusto Karmali, Amin Vieira, Manuela |
| author_role | author |
| contributor_name_str_mv | RCIPL |
| country_str | PT |
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| datacite.contributors.contributor.contributorName.fl_str_mv | RCIPL |
| datacite.creators.creator.creatorName.fl_str_mv | Louro, Paula Vaz da Silva, V Vieira, Manuel Augusto Karmali, Amin Vieira, Manuela |
| datacite.date.Accepted.fl_str_mv | 2013-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2013-10-20T18:52:50Z |
| datacite.date.embargoed.fl_str_mv | 2013-10-20T18:52:50Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Glucose sensing FRET Nanosensors Fluorescence proteins a-SiC:H photodiodes Multilayered heterostructures Optical sensors |
| datacite.titles.title.fl_str_mv | Use of a-SiC:H semiconductor-based transducer for glucose sensing through FRET analysis |
| dc.contributor.none.fl_str_mv | RCIPL |
| dc.creator.none.fl_str_mv | Louro, Paula Vaz da Silva, V Vieira, Manuel Augusto Karmali, Amin Vieira, Manuela |
| dc.date.Accepted.fl_str_mv | 2013-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2013-10-20T18:52:50Z |
| dc.date.embargoed.fl_str_mv | 2013-10-20T18:52:50Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.21/2759 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Springer-Verlag Berlin |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Glucose sensing FRET Nanosensors Fluorescence proteins a-SiC:H photodiodes Multilayered heterostructures Optical sensors |
| dc.title.fl_str_mv | Use of a-SiC:H semiconductor-based transducer for glucose sensing through FRET analysis |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | Glucose sensing is an issue with great interest in medical and biological applications. One possible approach to glucose detection takes advantage of measuring changes in fluorescence resonance energy transfer (FRET) between a fluorescent donor and an acceptor within a protein which undergoes glucose-induced changes in conformation. This demands the detection of fluorescent signals in the visible spectrum. In this paper we analyzed the emission spectrum obtained from fluorescent labels attached to a protein which changes its conformation in the presence of glucose using a commercial spectrofluorometer. Different glucose nanosensors were used to measure the output spectra with fluorescent signals located at the cyan and yellow bands of the spectrum. A new device is presented based on multilayered a-SiC:H heterostructures to detect identical transient visible signals. The transducer consists of a p-i'(a-SiC:H)-n/p-i(a-Si:H)-n heterostructure optimized for the detection of the fluorescence resonance energy transfer between fluorophores with excitation in the violet (400 nm) and emissions in the cyan (470 nm) and yellow (588 nm) range of the spectrum. Results show that the device photocurrent signal measured under reverse bias and using appropriate steady state optical bias, allows the separate detection of the cyan and yellow fluorescence signals presented. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | conferenceObject |
| fulltext.url.fl_str_mv | https://repositorio.ipl.pt/bitstreams/c7465d59-4b8f-433f-ae4c-57aa1761abf4/download |
| id | ripl_39a60ce1d9f08a4f17174bc09d88c98c |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.21/2759 |
| inst_facet_str | urn:organizationAcronym:ipl{{{_:::_}}}Universidade Politécnica de Lisboa |
| instacron_str | ipl |
| institution | Universidade Politécnica de Lisboa |
| instname_str | Universidade Politécnica de Lisboa |
| language | eng |
| network_acronym_str | ripl |
| network_name_str | Repositório Científico da Universidade Politécnica de Lisboa |
| oai_identifier_str | oai:repositorio.ipl.pt:10400.21/2759 |
| organization_str_mv | urn:organizationAcronym:ipl |
| person_str_mv | Louro, Paula Louro, Paula https://www.ciencia-id.pt/E511-8C08-E606 E511-8C08-E606 http://orcid.org/0000-0002-4167-2052 0000-0002-4167-2052 Vaz da Silva, V Vaz da Silva, V https://www.ciencia-id.pt/AB18-740E-B580 AB18-740E-B580 http://orcid.org/0000-0003-4265-6553 0000-0003-4265-6553 Vieira, Manuel Augusto Vieira, Manuel Augusto https://www.ciencia-id.pt/A511-1330-549F A511-1330-549F http://orcid.org/0000-0003-1385-3646 0000-0003-1385-3646 Karmali, Amin Karmali, Amin http://orcid.org/0000-0003-0419-401X 0000-0003-0419-401X Vieira, Manuela Vieira, Manuela https://www.ciencia-id.pt/9516-E25E-BB8E 9516-E25E-BB8E http://orcid.org/0000-0002-1150-9895 0000-0002-1150-9895 |
| publishDate | 2013 |
| publisher.none.fl_str_mv | Springer-Verlag Berlin |
| repo_facet_str | urn:repositoryAcronym:ripl{{{_:::_}}}Repositório Científico da Universidade Politécnica de Lisboa |
| reponame_str | Repositório Científico da Universidade Politécnica de Lisboa |
| repository_id_str | urn:repositoryAcronym:ripl |
| service_str_mv | urn:repositoryAcronym:ripl |
| spelling | engSpringer-Verlag BerlinporGlucose sensing is an issue with great interest in medical and biological applications. One possible approach to glucose detection takes advantage of measuring changes in fluorescence resonance energy transfer (FRET) between a fluorescent donor and an acceptor within a protein which undergoes glucose-induced changes in conformation. This demands the detection of fluorescent signals in the visible spectrum. In this paper we analyzed the emission spectrum obtained from fluorescent labels attached to a protein which changes its conformation in the presence of glucose using a commercial spectrofluorometer. Different glucose nanosensors were used to measure the output spectra with fluorescent signals located at the cyan and yellow bands of the spectrum. A new device is presented based on multilayered a-SiC:H heterostructures to detect identical transient visible signals. The transducer consists of a p-i'(a-SiC:H)-n/p-i(a-Si:H)-n heterostructure optimized for the detection of the fluorescence resonance energy transfer between fluorophores with excitation in the violet (400 nm) and emissions in the cyan (470 nm) and yellow (588 nm) range of the spectrum. Results show that the device photocurrent signal measured under reverse bias and using appropriate steady state optical bias, allows the separate detection of the cyan and yellow fluorescence signals presented.application/pdfporUse of a-SiC:H semiconductor-based transducer for glucose sensing through FRET analysisPersonalLouro, PaulaDSpacehttp://dspace.org/items/730dc3dd-73eb-428d-b3a6-430085e33585DSpacehttp://dspace.org/items/730dc3dd-73eb-428d-b3a6-430085e33585LouroPaulaCiência IDhttps://www.ciencia-id.ptE511-8C08-E606ORCIDhttp://orcid.org0000-0002-4167-2052Researcher IDhttps://www.researcherid.comU-8346-2017Scopus Author IDhttps://www.scopus.com8845716400PersonalVaz da Silva, VDSpacehttp://dspace.org/items/bf73931d-fd0b-44bc-82a9-fdfd2157406fDSpacehttp://dspace.org/items/bf73931d-fd0b-44bc-82a9-fdfd2157406fVaz da SilvaVitorCiência IDhttps://www.ciencia-id.ptAB18-740E-B580ORCIDhttp://orcid.org0000-0003-4265-6553PersonalVieira, Manuel AugustoDSpacehttp://dspace.org/items/d8ffd958-0f70-4ec1-a9e9-d18e5c6d308eDSpacehttp://dspace.org/items/d8ffd958-0f70-4ec1-a9e9-d18e5c6d308eAugusto VieiraManuelCiência IDhttps://www.ciencia-id.ptA511-1330-549FORCIDhttp://orcid.org0000-0003-1385-3646Scopus Author IDhttps://www.scopus.com5719 1567905PersonalKarmali, AminDSpacehttp://dspace.org/items/23975b4b-3234-4139-9675-89ea6f9a2332DSpacehttp://dspace.org/items/23975b4b-3234-4139-9675-89ea6f9a2332KarmaliAminORCIDhttp://orcid.org0000-0003-0419-401XResearcher IDhttps://www.researcherid.comE-4787-2011Scopus Author IDhttps://www.scopus.com6603778216PersonalVieira, ManuelaDSpacehttp://dspace.org/items/b77c6785-9cfb-4cd4-90b5-c2b816bd7d11DSpacehttp://dspace.org/items/b77c6785-9cfb-4cd4-90b5-c2b816bd7d11VieiraManuelaCiência IDhttps://www.ciencia-id.pt9516-E25E-BB8EORCIDhttp://orcid.org0000-0002-1150-9895Researcher IDhttps://www.researcherid.comV-7860-2017Scopus Author IDhttps://www.scopus.com7202140173Scopus Author IDhttps://www.scopus.com57202593765HostingInstitutionOrganizationalRCIPLe-mailmailto:rcaap@sp.ipl.ptrcaap@sp.ipl.ptISBNIsPartOf978-3-642-37290-2ISSNIsPartOf1868-42382013-10-20T18:52:50Z20132013-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.21/2759http://purl.org/coar/access_right/c_abf2open accessGlucose sensingFRETNanosensorsFluorescence proteinsa-SiC:H photodiodesMultilayered heterostructuresOptical sensors536398 bytesother research producthttp://purl.org/coar/resource_type/c_c94fconference objecthttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ipl.pt/bitstreams/c7465d59-4b8f-433f-ae4c-57aa1761abf4/downloadTechnological Innovation for the Internet of Things394631638Berlin |
| spellingShingle | Use of a-SiC:H semiconductor-based transducer for glucose sensing through FRET analysis Louro, Paula Glucose sensing FRET Nanosensors Fluorescence proteins a-SiC:H photodiodes Multilayered heterostructures Optical sensors |
| status | SINGLETON |
| subject.fl_str_mv | Glucose sensing FRET Nanosensors Fluorescence proteins a-SiC:H photodiodes Multilayered heterostructures Optical sensors |
| title | Use of a-SiC:H semiconductor-based transducer for glucose sensing through FRET analysis |
| title_full | Use of a-SiC:H semiconductor-based transducer for glucose sensing through FRET analysis |
| title_fullStr | Use of a-SiC:H semiconductor-based transducer for glucose sensing through FRET analysis |
| title_full_unstemmed | Use of a-SiC:H semiconductor-based transducer for glucose sensing through FRET analysis |
| title_short | Use of a-SiC:H semiconductor-based transducer for glucose sensing through FRET analysis |
| title_sort | Use of a-SiC:H semiconductor-based transducer for glucose sensing through FRET analysis |
| topic | Glucose sensing FRET Nanosensors Fluorescence proteins a-SiC:H photodiodes Multilayered heterostructures Optical sensors |
| topic_facet | Glucose sensing FRET Nanosensors Fluorescence proteins a-SiC:H photodiodes Multilayered heterostructures Optical sensors |
| url | http://hdl.handle.net/10400.21/2759 |
| visible | 1 |
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