Publicação
Designing highly customizable human based platforms for cell culture using proteins from the amniotic membrane
| Resumo: | In the past few years researchers have witnessed a paradigm shift in the development of biomaterials for drug discovery, tissue engineering, and regenerative medicine. After the great advances resulting from the transition of the 2D to the 3D, the new focus has been to increase the clinical relevance of such systems, as well as avoid the use of animals, by developing platforms that better replicate the human physiology in vitro. In this sense, we envisage the use of human matrices extracted from ethically sourced and readily available tissues as an optimal and promising alternative to currently used approaches. Hereupon, we report for the first time the chemical modification of human ECM proteins from the amniotic membrane (AM) with photoresponsive groups to produce bioinks and hydrogel precursors to engineer customizable platforms that are representative of native tissues and capable of supporting long-term cell culture. Our results demonstrated an efficient decellularization, liquefaction and functionalization of AM-derived ECM with methacryloyl domains (AMMA), with production of stable and versatile hydrogels. Mechanical characterization evidenced an increased compression strength as a function of methacrylation degree and decellularized ECM concentration. Three-dimensional (3D) stem cell culture in the AMMA hydrogels resulted in viable and proliferative cells up to 7 days; moreover, the mouldable character of the hydrogel precursors permits the processing of patterned hydrogel constructs allowing the control over cellular alignment and elongation, or microgels with highly tunable shape. |
|---|---|
| Autores principais: | Deus, Inês A. |
| Outros Autores: | Santos, Sara C.; Custódio, Catarina A.; Mano, João F. |
| Assunto: | Amniotic membrane Extracellular matrix Photocrosslinking Hydrogels 3D cell culture |
| Ano: | 2022 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Aveiro |
| Idioma: | inglês |
| Origem: | RIA - Repositório Institucional da Universidade de Aveiro |
| _version_ | 1877769223688683520 |
|---|---|
| author | Deus, Inês A. |
| author2 | Santos, Sara C. Custódio, Catarina A. Mano, João F. |
| author2_role | author author author |
| author_facet | Deus, Inês A. Santos, Sara C. Custódio, Catarina A. Mano, João F. |
| author_role | author |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Deus, Inês A.\"},{\"Person.name\":\"Santos, Sara C.\"},{\"Person.name\":\"Custódio, Catarina A.\"},{\"Person.name\":\"Mano, João F.\"}] |
| datacite.creators.creator.creatorName.fl_str_mv | Deus, Inês A. Santos, Sara C. Custódio, Catarina A. Mano, João F. |
| datacite.date.Accepted.fl_str_mv | 2022-03-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2025-06-13T10:44:42Z |
| datacite.date.embargoed.fl_str_mv | 2025-06-13T10:44:42Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Amniotic membrane Extracellular matrix Photocrosslinking Hydrogels 3D cell culture |
| datacite.titles.title.fl_str_mv | Designing highly customizable human based platforms for cell culture using proteins from the amniotic membrane |
| dc.creator.none.fl_str_mv | Deus, Inês A. Santos, Sara C. Custódio, Catarina A. Mano, João F. |
| dc.date.Accepted.fl_str_mv | 2022-03-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2025-06-13T10:44:42Z |
| dc.date.embargoed.fl_str_mv | 2025-06-13T10:44:42Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10773/34427 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Amniotic membrane Extracellular matrix Photocrosslinking Hydrogels 3D cell culture |
| dc.title.fl_str_mv | Designing highly customizable human based platforms for cell culture using proteins from the amniotic membrane |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | In the past few years researchers have witnessed a paradigm shift in the development of biomaterials for drug discovery, tissue engineering, and regenerative medicine. After the great advances resulting from the transition of the 2D to the 3D, the new focus has been to increase the clinical relevance of such systems, as well as avoid the use of animals, by developing platforms that better replicate the human physiology in vitro. In this sense, we envisage the use of human matrices extracted from ethically sourced and readily available tissues as an optimal and promising alternative to currently used approaches. Hereupon, we report for the first time the chemical modification of human ECM proteins from the amniotic membrane (AM) with photoresponsive groups to produce bioinks and hydrogel precursors to engineer customizable platforms that are representative of native tissues and capable of supporting long-term cell culture. Our results demonstrated an efficient decellularization, liquefaction and functionalization of AM-derived ECM with methacryloyl domains (AMMA), with production of stable and versatile hydrogels. Mechanical characterization evidenced an increased compression strength as a function of methacrylation degree and decellularized ECM concentration. Three-dimensional (3D) stem cell culture in the AMMA hydrogels resulted in viable and proliferative cells up to 7 days; moreover, the mouldable character of the hydrogel precursors permits the processing of patterned hydrogel constructs allowing the control over cellular alignment and elongation, or microgels with highly tunable shape. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ria_88ea538b6f28172f9aa39da3239a3baa |
| identifier.url.fl_str_mv | http://hdl.handle.net/10773/34427 |
| inst_facet_str | urn:organizationAcronym:ua{{{_:::_}}}Universidade de Aveiro |
| instacron_str | ua |
| institution | Universidade de Aveiro |
| instname_str | Universidade de Aveiro |
| language | eng |
| network_acronym_str | ria |
| network_name_str | RIA - Repositório Institucional da Universidade de Aveiro |
| oai_identifier_str | oai:ria.ua.pt:10773/34427 |
| organization_str_mv | urn:organizationAcronym:ua |
| person_str_mv | Deus, Inês A. Santos, Sara C. Custódio, Catarina A. Mano, João F. |
| publishDate | 2022 |
| publisher.none.fl_str_mv | Elsevier |
| repo_facet_str | urn:repositoryAcronym:ria{{{_:::_}}}RIA - Repositório Institucional da Universidade de Aveiro |
| reponame_str | RIA - Repositório Institucional da Universidade de Aveiro |
| repository_id_str | urn:repositoryAcronym:ria |
| service_str_mv | urn:repositoryAcronym:ria |
| spelling | pt_PTIn the past few years researchers have witnessed a paradigm shift in the development of biomaterials for drug discovery, tissue engineering, and regenerative medicine. After the great advances resulting from the transition of the 2D to the 3D, the new focus has been to increase the clinical relevance of such systems, as well as avoid the use of animals, by developing platforms that better replicate the human physiology in vitro. In this sense, we envisage the use of human matrices extracted from ethically sourced and readily available tissues as an optimal and promising alternative to currently used approaches. Hereupon, we report for the first time the chemical modification of human ECM proteins from the amniotic membrane (AM) with photoresponsive groups to produce bioinks and hydrogel precursors to engineer customizable platforms that are representative of native tissues and capable of supporting long-term cell culture. Our results demonstrated an efficient decellularization, liquefaction and functionalization of AM-derived ECM with methacryloyl domains (AMMA), with production of stable and versatile hydrogels. Mechanical characterization evidenced an increased compression strength as a function of methacrylation degree and decellularized ECM concentration. Three-dimensional (3D) stem cell culture in the AMMA hydrogels resulted in viable and proliferative cells up to 7 days; moreover, the mouldable character of the hydrogel precursors permits the processing of patterned hydrogel constructs allowing the control over cellular alignment and elongation, or microgels with highly tunable shape.application/pdfengElsevierpt_PTDesigning highly customizable human based platforms for cell culture using proteins from the amniotic membraneDeus, Inês A.Santos, Sara C.Custódio, Catarina A.Mano, João F.Handlehttp://hdl.handle.net/10773/34427DOIIsPartOf10.1016/j.msec.2021.1125742025-06-13T10:44:42Z2022-03-01T00:00:00Z2022-03http://purl.org/coar/access_right/c_abf2open accesspt_PTAmniotic membranept_PTExtracellular matrixpt_PTPhotocrosslinkingpt_PTHydrogelspt_PT3D cell culture5227660 byteshttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://ria.ua.pt/bitstream/10773/34427/1/Designing%20highly%20customizable%20human%20based%20platforms%20for%20cell%20culture%20using%20proteins%20from%20the%20amniotic%20membrane%20%28s%29.pdfliteraturehttp://purl.org/coar/resource_type/c_6501journal article |
| spellingShingle | Designing highly customizable human based platforms for cell culture using proteins from the amniotic membrane Deus, Inês A. Amniotic membrane Extracellular matrix Photocrosslinking Hydrogels 3D cell culture |
| status | SINGLETON |
| subject.fl_str_mv | Amniotic membrane Extracellular matrix Photocrosslinking Hydrogels 3D cell culture |
| title | Designing highly customizable human based platforms for cell culture using proteins from the amniotic membrane |
| title_full | Designing highly customizable human based platforms for cell culture using proteins from the amniotic membrane |
| title_fullStr | Designing highly customizable human based platforms for cell culture using proteins from the amniotic membrane |
| title_full_unstemmed | Designing highly customizable human based platforms for cell culture using proteins from the amniotic membrane |
| title_short | Designing highly customizable human based platforms for cell culture using proteins from the amniotic membrane |
| title_sort | Designing highly customizable human based platforms for cell culture using proteins from the amniotic membrane |
| topic | Amniotic membrane Extracellular matrix Photocrosslinking Hydrogels 3D cell culture |
| topic_facet | Amniotic membrane Extracellular matrix Photocrosslinking Hydrogels 3D cell culture |
| url | http://hdl.handle.net/10773/34427 |
| visible | 1 |
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