Publicação
Novel pectin and nanocellulose based bioinks for 3D bioprinting applications
| Resumo: | The implementation of personalized and efficient strategies for damaged tissue/organ replacement is becoming increasingly urgent, being observed significant advancements in the field of tissue engineering in recent years, namely in 3D bioprinting of functional living tissue analogues. This automatized technology allows a wide control over the cellular environment and the overall tissue organization. To obtain a fully developed and functional tissue/organ, the bioprinting method and the bioink need to have adequate properties and functionalities, for this reason, a thoughtful choice is crucial. Recently, there have been great developments in the field of bioinks for bioprinting applications. However, the developed bioinks still present several limitations, such as weak mechanical properties, low viability, or high production costs. In this context, the objective of this dissertation is the development of a pectinnanocellulose fibers hydrogel-based bioink laden with human keratinocyte cells (HaCaT cells) for 3D bioprinting. These biopolymers were chosen due to their great potential for 3D bioprinting applications. Pectin is biodegradable, hydrophilic, and can form hydrogels in the presence of divalent cations, such as calcium. Nanocellulose has also already demonstrated its potentialities in 3Dbioprinting, revealing excellent mechanical properties and biocompatibility. However, this combination of biopolymers has never been explored for 3Dbioprinting. The developed bioinks were characterized in terms of their rheology, chemical structure/composition, and morphology. Furthermore, the bioprinting parameters were optimized and the cytotoxicity of the hydrogels evaluated. Rheologically, the inks presented a shear-thinning behavior, which is extremely important for 3D bioprinting applications, furthermore, an increase of three levels of magnitude in viscosity and shear stress was observed after a pre-crosslinking procedure. The inks were able to be printed with the optimal result being obtained for the ink with the highest content of NFC and prereticulated with 1% (m/v) CaCl2, which allowed the printing of up to 8 layers without losing resolution. The fully-crosslinked hydrogels were considered noncytotoxic towards HaCaT cells, however higher cell viability values were observed for the hydrogels with higher NFC content. This study can be considered a great step towards the use of pectin-NFC-based bioinks in 3Dbioprinting applications. |
|---|---|
| Autores principais: | Guincho, Pedro Batalha |
| Assunto: | 3D-bioprinting Hydrogels Pectin Cellulose nanofibers Tissue engineering |
| Ano: | 2021 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Aveiro |
| Idioma: | inglês |
| Origem: | RIA - Repositório Institucional da Universidade de Aveiro |
| _version_ | 1877769128178089984 |
|---|---|
| author | Guincho, Pedro Batalha |
| author_facet | Guincho, Pedro Batalha |
| author_role | author |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Guincho, Pedro Batalha\"}] |
| datacite.creators.creator.creatorName.fl_str_mv | Guincho, Pedro Batalha |
| datacite.date.Accepted.fl_str_mv | 2021-02-15T00:00:00Z |
| datacite.date.available.fl_str_mv | 2025-06-13T10:44:59Z |
| datacite.date.embargoed.fl_str_mv | 2025-06-13T10:44:59Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | 3D-bioprinting Hydrogels Pectin Cellulose nanofibers Tissue engineering |
| datacite.titles.title.fl_str_mv | Novel pectin and nanocellulose based bioinks for 3D bioprinting applications |
| dc.creator.none.fl_str_mv | Guincho, Pedro Batalha |
| dc.date.Accepted.fl_str_mv | 2021-02-15T00:00:00Z |
| dc.date.available.fl_str_mv | 2025-06-13T10:44:59Z |
| dc.date.embargoed.fl_str_mv | 2025-06-13T10:44:59Z |
| dc.description.none.fl_str_mv | A implementação de estratégias personalizadas e eficientes para a regeneração de tecidos/órgãos danificados é cada vez mais importante, enos últimos anos têm-se observado avanços significativos no campo da engenharia de tecidos, nomeadamente, na bio-impressão 3D de análogos de tecidos vivos funcionais. Esta tecnologia automatizada permite o controlo do ambiente celular e também da organização tecidular. Para que o tecido impresso seja funcional e adequado para a aplicação em causa, o método de bio-impressão e a biotinta necessitam de ter propriedades e funcionalidades adequadas, sendo a sua seleção um passo fundamental. Recentemente, tem havido um grande progresso no desenvolvimentode biotintas para aplicação em bio-impressão3D. Contudo, as biotintas desenvolvidas ainda apresentam diversas limitações, tais como fracas propriedades mecânicas, baixa viabilidade celular ou custos de produção elevados. Neste contexto, o objetivo destadissertação é o desenvolvimento de uma biotinta à base de um hidrogel de pectina e nanofibras de celulose (NFC) com incorporação de queratinócitos humanos (células HaCaT) para aplicação em bio-impressão 3D. Estes biopolímeros foram escolhidos devido às suas propriedades intrínsecas e ao seu potencial para impressão 3D. A pectina é biodegradável, hidrofílica e forma hidrogéis na presença de catiões divalentes, como o cálcio. A nanocelulose também já demonstrou as suas potencialidades, revelando excelentes propriedades mecânicas e biocompatibilidade. Contudo, a combinação de pectina e NFC nunca fui explorada no campo da bio-impressão 3D.As tintas desenvolvidas foram caraterizadas em termos da sua reologia, estrutura/composição química e morfologia. Adicionalmente, os parâmetros de impressão foram otimizados e a citotoxicidade dos hidrogéis avaliada. Reologicamente, as tintas apresentaram um comportamento pseudoplástico, que é extremamente importante para aplicações em bio-impressão 3D e, além disso, após pré-reticulação observou-se um aumento de três níveis de magnitude na viscosidade e tensão de cisalhamento. As tintas desenvolvidas têm aptidão para serem imprimidas, tendo o resultado mais promissor sido obtido para a tinta com maior quantidade de NFC e pré-reticulada com1%(m/v) de CaCl2. Nomeadamente, conseguiu-se imprimiraté8 camadas sem perder resolução. Os hidrogéis imprimidos mostraram ser não citotóxicos para as células HaCaT, contudo os valores de viabilidade celular mais elevados foram observados para os hidrogéis com maiores quantidades de nanocelulose. Este estudo considera-se como um grande passo em direção ao uso de biotintas baseadas em pectina e NFC embio-impressão3D. |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10773/30763 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | 3D-bioprinting Hydrogels Pectin Cellulose nanofibers Tissue engineering |
| dc.title.fl_str_mv | Novel pectin and nanocellulose based bioinks for 3D bioprinting applications |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_bdcc |
| description | The implementation of personalized and efficient strategies for damaged tissue/organ replacement is becoming increasingly urgent, being observed significant advancements in the field of tissue engineering in recent years, namely in 3D bioprinting of functional living tissue analogues. This automatized technology allows a wide control over the cellular environment and the overall tissue organization. To obtain a fully developed and functional tissue/organ, the bioprinting method and the bioink need to have adequate properties and functionalities, for this reason, a thoughtful choice is crucial. Recently, there have been great developments in the field of bioinks for bioprinting applications. However, the developed bioinks still present several limitations, such as weak mechanical properties, low viability, or high production costs. In this context, the objective of this dissertation is the development of a pectinnanocellulose fibers hydrogel-based bioink laden with human keratinocyte cells (HaCaT cells) for 3D bioprinting. These biopolymers were chosen due to their great potential for 3D bioprinting applications. Pectin is biodegradable, hydrophilic, and can form hydrogels in the presence of divalent cations, such as calcium. Nanocellulose has also already demonstrated its potentialities in 3Dbioprinting, revealing excellent mechanical properties and biocompatibility. However, this combination of biopolymers has never been explored for 3Dbioprinting. The developed bioinks were characterized in terms of their rheology, chemical structure/composition, and morphology. Furthermore, the bioprinting parameters were optimized and the cytotoxicity of the hydrogels evaluated. Rheologically, the inks presented a shear-thinning behavior, which is extremely important for 3D bioprinting applications, furthermore, an increase of three levels of magnitude in viscosity and shear stress was observed after a pre-crosslinking procedure. The inks were able to be printed with the optimal result being obtained for the ink with the highest content of NFC and prereticulated with 1% (m/v) CaCl2, which allowed the printing of up to 8 layers without losing resolution. The fully-crosslinked hydrogels were considered noncytotoxic towards HaCaT cells, however higher cell viability values were observed for the hydrogels with higher NFC content. This study can be considered a great step towards the use of pectin-NFC-based bioinks in 3Dbioprinting applications. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | masterThesis |
| id | ria_57586259fc653dd967f5f46fbfa0de11 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10773/30763 |
| inst_facet_str | urn:organizationAcronym:ua{{{_:::_}}}Universidade de Aveiro |
| instacron_str | ua |
| institution | Universidade de Aveiro |
| instname_str | Universidade de Aveiro |
| language | eng |
| network_acronym_str | ria |
| network_name_str | RIA - Repositório Institucional da Universidade de Aveiro |
| oai_identifier_str | oai:ria.ua.pt:10773/30763 |
| organization_str_mv | urn:organizationAcronym:ua |
| person_str_mv | Guincho, Pedro Batalha |
| publishDate | 2021 |
| repo_facet_str | urn:repositoryAcronym:ria{{{_:::_}}}RIA - Repositório Institucional da Universidade de Aveiro |
| reponame_str | RIA - Repositório Institucional da Universidade de Aveiro |
| repository_id_str | urn:repositoryAcronym:ria |
| service_str_mv | urn:repositoryAcronym:ria |
| spelling | pt_PTThe implementation of personalized and efficient strategies for damaged tissue/organ replacement is becoming increasingly urgent, being observed significant advancements in the field of tissue engineering in recent years, namely in 3D bioprinting of functional living tissue analogues. This automatized technology allows a wide control over the cellular environment and the overall tissue organization. To obtain a fully developed and functional tissue/organ, the bioprinting method and the bioink need to have adequate properties and functionalities, for this reason, a thoughtful choice is crucial. Recently, there have been great developments in the field of bioinks for bioprinting applications. However, the developed bioinks still present several limitations, such as weak mechanical properties, low viability, or high production costs. In this context, the objective of this dissertation is the development of a pectinnanocellulose fibers hydrogel-based bioink laden with human keratinocyte cells (HaCaT cells) for 3D bioprinting. These biopolymers were chosen due to their great potential for 3D bioprinting applications. Pectin is biodegradable, hydrophilic, and can form hydrogels in the presence of divalent cations, such as calcium. Nanocellulose has also already demonstrated its potentialities in 3Dbioprinting, revealing excellent mechanical properties and biocompatibility. However, this combination of biopolymers has never been explored for 3Dbioprinting. The developed bioinks were characterized in terms of their rheology, chemical structure/composition, and morphology. Furthermore, the bioprinting parameters were optimized and the cytotoxicity of the hydrogels evaluated. Rheologically, the inks presented a shear-thinning behavior, which is extremely important for 3D bioprinting applications, furthermore, an increase of three levels of magnitude in viscosity and shear stress was observed after a pre-crosslinking procedure. The inks were able to be printed with the optimal result being obtained for the ink with the highest content of NFC and prereticulated with 1% (m/v) CaCl2, which allowed the printing of up to 8 layers without losing resolution. The fully-crosslinked hydrogels were considered noncytotoxic towards HaCaT cells, however higher cell viability values were observed for the hydrogels with higher NFC content. This study can be considered a great step towards the use of pectin-NFC-based bioinks in 3Dbioprinting applications.pt_PTA implementação de estratégias personalizadas e eficientes para a regeneração de tecidos/órgãos danificados é cada vez mais importante, enos últimos anos têm-se observado avanços significativos no campo da engenharia de tecidos, nomeadamente, na bio-impressão 3D de análogos de tecidos vivos funcionais. Esta tecnologia automatizada permite o controlo do ambiente celular e também da organização tecidular. Para que o tecido impresso seja funcional e adequado para a aplicação em causa, o método de bio-impressão e a biotinta necessitam de ter propriedades e funcionalidades adequadas, sendo a sua seleção um passo fundamental. Recentemente, tem havido um grande progresso no desenvolvimentode biotintas para aplicação em bio-impressão3D. Contudo, as biotintas desenvolvidas ainda apresentam diversas limitações, tais como fracas propriedades mecânicas, baixa viabilidade celular ou custos de produção elevados. Neste contexto, o objetivo destadissertação é o desenvolvimento de uma biotinta à base de um hidrogel de pectina e nanofibras de celulose (NFC) com incorporação de queratinócitos humanos (células HaCaT) para aplicação em bio-impressão 3D. Estes biopolímeros foram escolhidos devido às suas propriedades intrínsecas e ao seu potencial para impressão 3D. A pectina é biodegradável, hidrofílica e forma hidrogéis na presença de catiões divalentes, como o cálcio. A nanocelulose também já demonstrou as suas potencialidades, revelando excelentes propriedades mecânicas e biocompatibilidade. Contudo, a combinação de pectina e NFC nunca fui explorada no campo da bio-impressão 3D.As tintas desenvolvidas foram caraterizadas em termos da sua reologia, estrutura/composição química e morfologia. Adicionalmente, os parâmetros de impressão foram otimizados e a citotoxicidade dos hidrogéis avaliada. Reologicamente, as tintas apresentaram um comportamento pseudoplástico, que é extremamente importante para aplicações em bio-impressão 3D e, além disso, após pré-reticulação observou-se um aumento de três níveis de magnitude na viscosidade e tensão de cisalhamento. As tintas desenvolvidas têm aptidão para serem imprimidas, tendo o resultado mais promissor sido obtido para a tinta com maior quantidade de NFC e pré-reticulada com1%(m/v) de CaCl2. Nomeadamente, conseguiu-se imprimiraté8 camadas sem perder resolução. Os hidrogéis imprimidos mostraram ser não citotóxicos para as células HaCaT, contudo os valores de viabilidade celular mais elevados foram observados para os hidrogéis com maiores quantidades de nanocelulose. Este estudo considera-se como um grande passo em direção ao uso de biotintas baseadas em pectina e NFC embio-impressão3D.application/pdfengpt_PTNovel pectin and nanocellulose based bioinks for 3D bioprinting applicationsGuincho, Pedro BatalhaHandlehttp://hdl.handle.net/10773/307632025-06-13T10:44:59Z2021-02-15T00:00:00Z2021-02-15http://purl.org/coar/access_right/c_abf2open accesspt_PT3D-bioprintingpt_PTHydrogelspt_PTPectinpt_PTCellulose nanofiberspt_PTTissue engineering3011928 byteshttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://ria.ua.pt/bitstream/10773/30763/1/Documento_Pedro_Guincho.pdfother research producthttp://purl.org/coar/resource_type/c_bdccmaster thesis |
| spellingShingle | Novel pectin and nanocellulose based bioinks for 3D bioprinting applications Guincho, Pedro Batalha 3D-bioprinting Hydrogels Pectin Cellulose nanofibers Tissue engineering |
| status | SINGLETON |
| subject.fl_str_mv | 3D-bioprinting Hydrogels Pectin Cellulose nanofibers Tissue engineering |
| title | Novel pectin and nanocellulose based bioinks for 3D bioprinting applications |
| title_full | Novel pectin and nanocellulose based bioinks for 3D bioprinting applications |
| title_fullStr | Novel pectin and nanocellulose based bioinks for 3D bioprinting applications |
| title_full_unstemmed | Novel pectin and nanocellulose based bioinks for 3D bioprinting applications |
| title_short | Novel pectin and nanocellulose based bioinks for 3D bioprinting applications |
| title_sort | Novel pectin and nanocellulose based bioinks for 3D bioprinting applications |
| topic | 3D-bioprinting Hydrogels Pectin Cellulose nanofibers Tissue engineering |
| topic_facet | 3D-bioprinting Hydrogels Pectin Cellulose nanofibers Tissue engineering |
| url | http://hdl.handle.net/10773/30763 |
| visible | 1 |
Atividades financiadas
Não foram encontrados projetos financiados.