Publicação
Benefits in the macrophage response due to graphene oxide reduction by thermal treatment
| Resumo: | Graphene and its derivatives are very promising nanomaterials for biomedical applications and are proving to be very useful for the preparation of scaffolds for tissue repair. The response of immune cells to these graphene-based materials (GBM) appears to be critical in promoting regeneration, thus, the study of this response is essential before they are used to prepare any type of scaffold. Another relevant factor is the variability of the GBM surface chemistry, namely the type and quantity of oxygen functional groups, which may have an important effect on cell behavior. The response of RAW-264.7 macrophages to graphene oxide (GO) and two types of reduced GO, rGO15 and rGO30, obtained after vacuum-assisted thermal treatment of 15 and 30 min, respectively, was evaluated by analyzing the uptake of these nanostructures, the intracellular content of reactive oxygen species, and specific markers of the proinflammatory M1 phenotype, such as CD80 expression and secretion of inflammatory cytokines TNF-α and IL-6. Our results demonstrate that GO reduction resulted in a decrease of both oxidative stress and proinflammatory cytokine secretion, significantly improving its biocompatibility and potential for the preparation of 3D scaffolds able of triggering the appropriate immune response for tissue regeneration. |
|---|---|
| Autores principais: | Cicuéndez, Mónica |
| Outros Autores: | Casarrubios, Laura; Barroca, Natália; Silva, Daniela; Feito, María José; Diez-Orejas, Rosalía; Marques, Paula A. A. P.; Portolés, María Teresa |
| Assunto: | Cytokine Graphene oxide Immune response Macrophage Reduced graphene oxide |
| Ano: | 2021 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Aveiro |
| Idioma: | inglês |
| Origem: | RIA - Repositório Institucional da Universidade de Aveiro |
| _version_ | 1877769200694460416 |
|---|---|
| author | Cicuéndez, Mónica |
| author2 | Casarrubios, Laura Barroca, Natália Silva, Daniela Feito, María José Diez-Orejas, Rosalía Marques, Paula A. A. P. Portolés, María Teresa |
| author2_role | author author author author author author author |
| author_facet | Cicuéndez, Mónica Casarrubios, Laura Barroca, Natália Silva, Daniela Feito, María José Diez-Orejas, Rosalía Marques, Paula A. A. P. Portolés, María Teresa |
| author_role | author |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Cicuéndez, Mónica\"},{\"Person.name\":\"Casarrubios, Laura\"},{\"Person.name\":\"Barroca, Natália\"},{\"Person.name\":\"Silva, Daniela\"},{\"Person.name\":\"Feito, María José\"},{\"Person.name\":\"Diez-Orejas, Rosalía\"},{\"Person.name\":\"Marques, Paula A. A. P.\"},{\"Person.name\":\"Portolés, María Teresa\"}] |
| datacite.creators.creator.creatorName.fl_str_mv | Cicuéndez, Mónica Casarrubios, Laura Barroca, Natália Silva, Daniela Feito, María José Diez-Orejas, Rosalía Marques, Paula A. A. P. Portolés, María Teresa |
| datacite.date.Accepted.fl_str_mv | 2021-07-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2022-07-26T16:21:58Z |
| datacite.date.embargoed.fl_str_mv | 2022-07-26T16:21:58Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Cytokine Graphene oxide Immune response Macrophage Reduced graphene oxide |
| datacite.titles.title.fl_str_mv | Benefits in the macrophage response due to graphene oxide reduction by thermal treatment |
| dc.creator.none.fl_str_mv | Cicuéndez, Mónica Casarrubios, Laura Barroca, Natália Silva, Daniela Feito, María José Diez-Orejas, Rosalía Marques, Paula A. A. P. Portolés, María Teresa |
| dc.date.Accepted.fl_str_mv | 2021-07-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2022-07-26T16:21:58Z |
| dc.date.embargoed.fl_str_mv | 2022-07-26T16:21:58Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10773/34310 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | MDPI |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Cytokine Graphene oxide Immune response Macrophage Reduced graphene oxide |
| dc.title.fl_str_mv | Benefits in the macrophage response due to graphene oxide reduction by thermal treatment |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Graphene and its derivatives are very promising nanomaterials for biomedical applications and are proving to be very useful for the preparation of scaffolds for tissue repair. The response of immune cells to these graphene-based materials (GBM) appears to be critical in promoting regeneration, thus, the study of this response is essential before they are used to prepare any type of scaffold. Another relevant factor is the variability of the GBM surface chemistry, namely the type and quantity of oxygen functional groups, which may have an important effect on cell behavior. The response of RAW-264.7 macrophages to graphene oxide (GO) and two types of reduced GO, rGO15 and rGO30, obtained after vacuum-assisted thermal treatment of 15 and 30 min, respectively, was evaluated by analyzing the uptake of these nanostructures, the intracellular content of reactive oxygen species, and specific markers of the proinflammatory M1 phenotype, such as CD80 expression and secretion of inflammatory cytokines TNF-α and IL-6. Our results demonstrate that GO reduction resulted in a decrease of both oxidative stress and proinflammatory cytokine secretion, significantly improving its biocompatibility and potential for the preparation of 3D scaffolds able of triggering the appropriate immune response for tissue regeneration. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ria_4db7dd537601c3bf2e8d2539fd2254f8 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10773/34310 |
| inst_facet_str | urn:organizationAcronym:ua{{{_:::_}}}Universidade de Aveiro |
| instacron_str | ua |
| institution | Universidade de Aveiro |
| instname_str | Universidade de Aveiro |
| language | eng |
| network_acronym_str | ria |
| network_name_str | RIA - Repositório Institucional da Universidade de Aveiro |
| oai_identifier_str | oai:ria.ua.pt:10773/34310 |
| organization_str_mv | urn:organizationAcronym:ua |
| person_str_mv | Cicuéndez, Mónica Casarrubios, Laura Barroca, Natália Silva, Daniela Feito, María José Diez-Orejas, Rosalía Marques, Paula A. A. P. Portolés, María Teresa |
| publishDate | 2021 |
| publisher.none.fl_str_mv | MDPI |
| repo_facet_str | urn:repositoryAcronym:ria{{{_:::_}}}RIA - Repositório Institucional da Universidade de Aveiro |
| reponame_str | RIA - Repositório Institucional da Universidade de Aveiro |
| repository_id_str | urn:repositoryAcronym:ria |
| service_str_mv | urn:repositoryAcronym:ria |
| spelling | pt_PTGraphene and its derivatives are very promising nanomaterials for biomedical applications and are proving to be very useful for the preparation of scaffolds for tissue repair. The response of immune cells to these graphene-based materials (GBM) appears to be critical in promoting regeneration, thus, the study of this response is essential before they are used to prepare any type of scaffold. Another relevant factor is the variability of the GBM surface chemistry, namely the type and quantity of oxygen functional groups, which may have an important effect on cell behavior. The response of RAW-264.7 macrophages to graphene oxide (GO) and two types of reduced GO, rGO15 and rGO30, obtained after vacuum-assisted thermal treatment of 15 and 30 min, respectively, was evaluated by analyzing the uptake of these nanostructures, the intracellular content of reactive oxygen species, and specific markers of the proinflammatory M1 phenotype, such as CD80 expression and secretion of inflammatory cytokines TNF-α and IL-6. Our results demonstrate that GO reduction resulted in a decrease of both oxidative stress and proinflammatory cytokine secretion, significantly improving its biocompatibility and potential for the preparation of 3D scaffolds able of triggering the appropriate immune response for tissue regeneration.application/pdfengMDPIpt_PTBenefits in the macrophage response due to graphene oxide reduction by thermal treatmentCicuéndez, MónicaCasarrubios, LauraBarroca, NatáliaSilva, DanielaFeito, María JoséDiez-Orejas, RosalíaMarques, Paula A. A. P.Portolés, María TeresaHandlehttp://hdl.handle.net/10773/34310ISSNIsPartOf1661-6596DOIIsPartOf10.3390/ijms221367012022-07-26T16:21:58Z2021-07-01T00:00:00Z2021-07-01http://purl.org/coar/access_right/c_abf2open accesspt_PTCytokinept_PTGraphene oxidept_PTImmune responsept_PTMacrophagept_PTReduced graphene oxide2915497 byteshttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://ria.ua.pt/bitstream/10773/34310/1/101-Int.%20J.%20Mol.%20Sci.%202021%2c%2022%2c%206701.pdfliteraturehttp://purl.org/coar/resource_type/c_6501journal article |
| spellingShingle | Benefits in the macrophage response due to graphene oxide reduction by thermal treatment Cicuéndez, Mónica Cytokine Graphene oxide Immune response Macrophage Reduced graphene oxide |
| status | SINGLETON |
| subject.fl_str_mv | Cytokine Graphene oxide Immune response Macrophage Reduced graphene oxide |
| title | Benefits in the macrophage response due to graphene oxide reduction by thermal treatment |
| title_full | Benefits in the macrophage response due to graphene oxide reduction by thermal treatment |
| title_fullStr | Benefits in the macrophage response due to graphene oxide reduction by thermal treatment |
| title_full_unstemmed | Benefits in the macrophage response due to graphene oxide reduction by thermal treatment |
| title_short | Benefits in the macrophage response due to graphene oxide reduction by thermal treatment |
| title_sort | Benefits in the macrophage response due to graphene oxide reduction by thermal treatment |
| topic | Cytokine Graphene oxide Immune response Macrophage Reduced graphene oxide |
| topic_facet | Cytokine Graphene oxide Immune response Macrophage Reduced graphene oxide |
| url | http://hdl.handle.net/10773/34310 |
| visible | 1 |
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