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Chemical modification of human proteins for the development of personalized cell culture and bioinks

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Summary:Tissue engineering (TE) aims to repair or regenerate tissues by combining knowledge from several research fields with advanced technologies like three-dimensional (3D) bioprinting. To overcome the cellular monolayer limitations, advances in protein-based bioinks are being developed. Proteins are one of the most promising biomaterials due to their biocompatibility, unique structure, complexity, abundance, and variety. Their structures make proteins prestigious chemical targets for modification, assisting in developing currently used bioinks. Envisioning to development of physiologically enhanced 3D cell culture platforms, this work presents chemically engineered human platelet lysates to develop a personalised bioink. The PL-DBCO-MA bioink was formed through two main reactions: the Strain-promoted azide-alkyne cycloaddition (SPAAC) reaction between the dibenzocyclooctyne (DBCO) and the azide-bearing Poly(ethylene glycol) methyl ether (PEG), and the photopolymerization between the methacrylic anhydride (MA) groups. Due to these two crosslinks, it was possible to have a system that gained viscosity and suitable characteristics for extrusion printing, and then the final construct was fixated by blue light. Mechanical and rheological assays proved the tunable characteristics for bioprinting and cell support. Human adipose stem cells (hASCs) encapsulation showed good biocompatibility and cell support of the biomaterial. Our findings represent the first successful development of a 3D printable human platelet lysates (hPL)-based bioink with no supporting bath.
Main Authors:Ribas, Tânia Isabel de Sousa
Subject:Tissue engineering Biomaterials Bioinks Human proteins Bioprinting
Year:2022
Country:Portugal
Document type:master thesis
Access type:open access
Associated institution:Universidade de Aveiro
Language:English
Origin:RIA - Repositório Institucional da Universidade de Aveiro

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