Publication
Development of antimicrobial polysaccharide-based wound dressings
| Summary: | Effective management of wounds is essential for preventing infections and promoting skin regeneration. Despite significant technological advances, many commercially available dressings exhibit limitations in exudate absorption, traumatic removal, and antimicrobial efficacy. In this context, polysaccharides have emerged as promising materials for the development of bioactive dressings, owing to their biocompatibility, biodegradability, and ability to form flexible films. This dissertation aimed to develop starch-based dressings plasticized with glycerol and doped with cationic porphyrins (TMePyP and TPPh3PyP), with the goal of combining suitable structural characteristics with antimicrobial photodynamic activity. The films were prepared by solvent casting and characterized in terms of physicochemical (thickness, wettability, residual moisture content, solubility, and singlet oxygen generation), mechanical (tensile strength, Young’s modulus, and elongation at break), and biological properties (antimicrobial activity against Staphylococcus aureus and Pseudomonas aeruginosa). Furthermore, the leaching behavior of the porphyrins in aqueous and biological media evaluated to support the interpretation of the biological performance results. The results obtained demonstrated that films doped with TMePyP exhibited greater flexibility, adequate wettability, and rapid release of the compound, suggesting potential applicability in the treatment of acute infections. Conversely, films with TPPh3PyP higher mechanical strength, lower solubility, and a more sustained release profile, features that may be advantageous for managing chronic infections. Films doped with porphyrins showed a high capacity for singlet oxygen generation under visible light irradiation and to significantly reduce bacterial viability in antimicrobial photodynamic therapy assays. These findings support the potential of these materials as promising photoactive platforms for antimicrobial wound dressings, adaptable to various clinical scenarios. |
|---|---|
| Main Authors: | Trindade, Patrícia Rafael |
| Subject: | Wound dressings Porphyrins Photodynamic therapy Starch films Antimicrobial materials Controlled release |
| Year: | 2025 |
| Country: | Portugal |
| Document type: | master thesis |
| Access type: | open access |
| Associated institution: | Universidade de Aveiro |
| Language: | English |
| Origin: | RIA - Repositório Institucional da Universidade de Aveiro |
| _version_ | 1877769479593656320 |
|---|---|
| author | Trindade, Patrícia Rafael |
| author_facet | Trindade, Patrícia Rafael |
| author_role | author |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Trindade, Patrícia Rafael\"}] |
| datacite.creators.creator.creatorName.fl_str_mv | Trindade, Patrícia Rafael |
| datacite.date.Accepted.fl_str_mv | 2025-07-16T00:00:00Z |
| datacite.date.available.fl_str_mv | 2025-10-09T14:55:30Z |
| datacite.date.embargoed.fl_str_mv | 2025-10-09T14:55:30Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Wound dressings Porphyrins Photodynamic therapy Starch films Antimicrobial materials Controlled release |
| datacite.titles.title.fl_str_mv | Development of antimicrobial polysaccharide-based wound dressings |
| dc.creator.none.fl_str_mv | Trindade, Patrícia Rafael |
| dc.date.Accepted.fl_str_mv | 2025-07-16T00:00:00Z |
| dc.date.available.fl_str_mv | 2025-10-09T14:55:30Z |
| dc.date.embargoed.fl_str_mv | 2025-10-09T14:55:30Z |
| dc.description.none.fl_str_mv | A gestão eficaz de feridas é essencial para a prevenção de infeções e para a promoção da regeneração cutânea. Não obstante os progressos tecnológicos, muitos pensos atualmente disponíveis apresentam limitações relativamente à absorção de exsudado, remoção traumática e eficácia antimicrobiana. Neste âmbito, os polissacarídeos emergem como materiais promissores para o desenvolvimento de pensos bioativos, devido à sua biocompatibilidade, biodegradabilidade e capacidade de formação de filmes flexíveis. A presente dissertação teve como objetivo o desenvolvimento de pensos à base de amido, plastificados com glicerol e dopados com porfirinas catiónicas (TMePyP e TPPh3PyP) visando a combinação de propriedades estruturais adequadas com atividade fotodinâmica antimicrobiana. Os filmes foram preparados por evaporação do solvente e caracterizados quanto às suas propriedades físico-químicas (espessura, molhabilidade, humidade residual, solubilidade, geração de oxigénio singleto), mecânicas (resistência à tração, módulo de Young e alongamento), e biológicas (atividade antimicrobiana contra Staphylococcus aureus e Pseudomonas aeruginosa). Adicionalmente, a lixiviação dos compostos em meios aquosos e biológicos foi objeto de teste, contribuindo para a validação dos resultados da avaliação biológica. Os resultados obtidos demonstraram que os filmes dopados com TMePyP apresentaram maior flexibilidade, molhabilidade adequada e rápida do composto nos primeiros minutos de contacto com meio aquoso, sugerindo um potencial para aplicação em infeções agudas, devido às suas características estruturais e de libertação do agente ativo. Em contraste, os filmes com TPPh3PyP exibiram maior resistência mecânica, menor solubilidade e libertação controlada, que sugerem potencial aplicação em infeções crónicas. Ambas as porfirinas exibiram uma elevada capacidade de gerar oxigénio singleto sob irradiação com luz visível e de reduzir significativamente a viabilidade bacteriana em ensaios de terapia fotodinâmica antimicrobiana. Os dados obtidos corroboram a aplicabilidade destes sistemas como plataformas promissoras para pensos antimicrobianos fotoativos, com potencial de adaptação a diferentes cenários clínicos. |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10773/45492 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Wound dressings Porphyrins Photodynamic therapy Starch films Antimicrobial materials Controlled release |
| dc.title.fl_str_mv | Development of antimicrobial polysaccharide-based wound dressings |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_bdcc |
| description | Effective management of wounds is essential for preventing infections and promoting skin regeneration. Despite significant technological advances, many commercially available dressings exhibit limitations in exudate absorption, traumatic removal, and antimicrobial efficacy. In this context, polysaccharides have emerged as promising materials for the development of bioactive dressings, owing to their biocompatibility, biodegradability, and ability to form flexible films. This dissertation aimed to develop starch-based dressings plasticized with glycerol and doped with cationic porphyrins (TMePyP and TPPh3PyP), with the goal of combining suitable structural characteristics with antimicrobial photodynamic activity. The films were prepared by solvent casting and characterized in terms of physicochemical (thickness, wettability, residual moisture content, solubility, and singlet oxygen generation), mechanical (tensile strength, Young’s modulus, and elongation at break), and biological properties (antimicrobial activity against Staphylococcus aureus and Pseudomonas aeruginosa). Furthermore, the leaching behavior of the porphyrins in aqueous and biological media evaluated to support the interpretation of the biological performance results. The results obtained demonstrated that films doped with TMePyP exhibited greater flexibility, adequate wettability, and rapid release of the compound, suggesting potential applicability in the treatment of acute infections. Conversely, films with TPPh3PyP higher mechanical strength, lower solubility, and a more sustained release profile, features that may be advantageous for managing chronic infections. Films doped with porphyrins showed a high capacity for singlet oxygen generation under visible light irradiation and to significantly reduce bacterial viability in antimicrobial photodynamic therapy assays. These findings support the potential of these materials as promising photoactive platforms for antimicrobial wound dressings, adaptable to various clinical scenarios. |
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| eu_rights_str_mv | openAccess |
| format | masterThesis |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10773/45492 |
| inst_facet_str | urn:organizationAcronym:ua{{{_:::_}}}Universidade de Aveiro |
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| institution | Universidade de Aveiro |
| instname_str | Universidade de Aveiro |
| language | eng |
| network_acronym_str | ria |
| network_name_str | RIA - Repositório Institucional da Universidade de Aveiro |
| oai_identifier_str | oai:ria.ua.pt:10773/45492 |
| organization_str_mv | urn:organizationAcronym:ua |
| person_str_mv | Trindade, Patrícia Rafael |
| publishDate | 2025 |
| repo_facet_str | urn:repositoryAcronym:ria{{{_:::_}}}RIA - Repositório Institucional da Universidade de Aveiro |
| reponame_str | RIA - Repositório Institucional da Universidade de Aveiro |
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| spelling | pt_PTEffective management of wounds is essential for preventing infections and promoting skin regeneration. Despite significant technological advances, many commercially available dressings exhibit limitations in exudate absorption, traumatic removal, and antimicrobial efficacy. In this context, polysaccharides have emerged as promising materials for the development of bioactive dressings, owing to their biocompatibility, biodegradability, and ability to form flexible films. This dissertation aimed to develop starch-based dressings plasticized with glycerol and doped with cationic porphyrins (TMePyP and TPPh3PyP), with the goal of combining suitable structural characteristics with antimicrobial photodynamic activity. The films were prepared by solvent casting and characterized in terms of physicochemical (thickness, wettability, residual moisture content, solubility, and singlet oxygen generation), mechanical (tensile strength, Young’s modulus, and elongation at break), and biological properties (antimicrobial activity against Staphylococcus aureus and Pseudomonas aeruginosa). Furthermore, the leaching behavior of the porphyrins in aqueous and biological media evaluated to support the interpretation of the biological performance results. The results obtained demonstrated that films doped with TMePyP exhibited greater flexibility, adequate wettability, and rapid release of the compound, suggesting potential applicability in the treatment of acute infections. Conversely, films with TPPh3PyP higher mechanical strength, lower solubility, and a more sustained release profile, features that may be advantageous for managing chronic infections. Films doped with porphyrins showed a high capacity for singlet oxygen generation under visible light irradiation and to significantly reduce bacterial viability in antimicrobial photodynamic therapy assays. These findings support the potential of these materials as promising photoactive platforms for antimicrobial wound dressings, adaptable to various clinical scenarios.pt_PTA gestão eficaz de feridas é essencial para a prevenção de infeções e para a promoção da regeneração cutânea. Não obstante os progressos tecnológicos, muitos pensos atualmente disponíveis apresentam limitações relativamente à absorção de exsudado, remoção traumática e eficácia antimicrobiana. Neste âmbito, os polissacarídeos emergem como materiais promissores para o desenvolvimento de pensos bioativos, devido à sua biocompatibilidade, biodegradabilidade e capacidade de formação de filmes flexíveis. A presente dissertação teve como objetivo o desenvolvimento de pensos à base de amido, plastificados com glicerol e dopados com porfirinas catiónicas (TMePyP e TPPh3PyP) visando a combinação de propriedades estruturais adequadas com atividade fotodinâmica antimicrobiana. Os filmes foram preparados por evaporação do solvente e caracterizados quanto às suas propriedades físico-químicas (espessura, molhabilidade, humidade residual, solubilidade, geração de oxigénio singleto), mecânicas (resistência à tração, módulo de Young e alongamento), e biológicas (atividade antimicrobiana contra Staphylococcus aureus e Pseudomonas aeruginosa). Adicionalmente, a lixiviação dos compostos em meios aquosos e biológicos foi objeto de teste, contribuindo para a validação dos resultados da avaliação biológica. Os resultados obtidos demonstraram que os filmes dopados com TMePyP apresentaram maior flexibilidade, molhabilidade adequada e rápida do composto nos primeiros minutos de contacto com meio aquoso, sugerindo um potencial para aplicação em infeções agudas, devido às suas características estruturais e de libertação do agente ativo. Em contraste, os filmes com TPPh3PyP exibiram maior resistência mecânica, menor solubilidade e libertação controlada, que sugerem potencial aplicação em infeções crónicas. Ambas as porfirinas exibiram uma elevada capacidade de gerar oxigénio singleto sob irradiação com luz visível e de reduzir significativamente a viabilidade bacteriana em ensaios de terapia fotodinâmica antimicrobiana. Os dados obtidos corroboram a aplicabilidade destes sistemas como plataformas promissoras para pensos antimicrobianos fotoativos, com potencial de adaptação a diferentes cenários clínicos.application/pdfengpt_PTDevelopment of antimicrobial polysaccharide-based wound dressingsTrindade, Patrícia RafaelHandlehttp://hdl.handle.net/10773/454922025-10-09T14:55:30Z2025-07-16T00:00:00Z2025-07-16http://purl.org/coar/access_right/c_abf2open accesspt_PTWound dressingspt_PTPorphyrinspt_PTPhotodynamic therapypt_PTStarch filmspt_PTAntimicrobial materialspt_PTControlled release1786614 byteshttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://ria.ua.pt/bitstream/10773/45492/1/Documento_Patr%c3%adcia_Trindade.pdfother research producthttp://purl.org/coar/resource_type/c_bdccmaster thesis |
| spellingShingle | Development of antimicrobial polysaccharide-based wound dressings Trindade, Patrícia Rafael Wound dressings Porphyrins Photodynamic therapy Starch films Antimicrobial materials Controlled release |
| status | SINGLETON |
| subject.fl_str_mv | Wound dressings Porphyrins Photodynamic therapy Starch films Antimicrobial materials Controlled release |
| title | Development of antimicrobial polysaccharide-based wound dressings |
| title_full | Development of antimicrobial polysaccharide-based wound dressings |
| title_fullStr | Development of antimicrobial polysaccharide-based wound dressings |
| title_full_unstemmed | Development of antimicrobial polysaccharide-based wound dressings |
| title_short | Development of antimicrobial polysaccharide-based wound dressings |
| title_sort | Development of antimicrobial polysaccharide-based wound dressings |
| topic | Wound dressings Porphyrins Photodynamic therapy Starch films Antimicrobial materials Controlled release |
| topic_facet | Wound dressings Porphyrins Photodynamic therapy Starch films Antimicrobial materials Controlled release |
| url | http://hdl.handle.net/10773/45492 |
| visible | 1 |
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