Publication
New insights of phytochemical profile and in vitro antioxidant and neuroprotective activities from optimized extract of Horned Melon fruit
| Summary: | This study aimed the optimization of antioxidant activity, total phenolic compounds (TPC) and total carotenoids of Horned Melon fruit submitted to maceration extraction, using ethanol as solvent. Optimization was performed by Box–Behnken design on three levels and three variables: extraction temperature (25 °C, 55 °C, and 85 °C), extraction time (0.5, 2.0, and 3.5 h) and ethanol concentration (10%, 50%, and 90%). The optimal conditions to improve the carotenoids extraction was 55 °C, 1.5 h and 86% ethanol, whereas the antiradical/antioxidant activity (ABTS and FRAP assays) and TPC were enhanced at 45 °C for 2.0 h and 40% ethanol. The optimal antioxidant extract was characterized regarding the phenolic composition and in vitro neuroprotective activities. The phenolic composition revealed the presence of high amounts of gallic acid (11.7 ± 0.6 mg/100 g dw) and protocatechuic acid (7.69 ± 0.38 mg/100 g dw). However, this extract presented a weak activity against Monoamine oxidase A (11.8 ± 2.3% inhibition at 2.7 mg/mL) and did not display anti-cholinesterase activity at the maximum concentration tested (1.3 mg/mL). Maceration was an efficient extraction technique to recover polyphenols and carotenoids from Horned Melon fruit. The optimized model can be very useful for food and pharmaceutical industries |
|---|---|
| Main Authors: | Vieira, Elsa F. |
| Other Authors: | Podlasiak, Martyna; Moreira, Manuela M.; Grosso, Clara; Rodrigues, Francisca; Fernandes, Virgínia Cruz; Delerue-Matos, Cristina |
| Subject: | Horned Melon fruit Box–Behnken design Maceration Phenolics Neuroprotective activity |
| Year: | 2022 |
| Country: | Portugal |
| Document type: | article |
| Access type: | restricted access |
| Associated institution: | Instituto Politécnico do Porto |
| Language: | English |
| Origin: | Repositório Científico do Instituto Politécnico do Porto |
| _version_ | 1877953161993388032 |
|---|---|
| author | Vieira, Elsa F. |
| author2 | Podlasiak, Martyna Moreira, Manuela M. Grosso, Clara Rodrigues, Francisca Fernandes, Virgínia Cruz Delerue-Matos, Cristina |
| author2_role | author author author author author author |
| author_facet | Vieira, Elsa F. Podlasiak, Martyna Moreira, Manuela M. Grosso, Clara Rodrigues, Francisca Fernandes, Virgínia Cruz Delerue-Matos, Cristina |
| author_role | author |
| contributor_name_str_mv | REPOSITÓRIO P.PORTO |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Vieira, Elsa F.\",\"Person.identifier.cienciaID\":\"D41C-E7B7-92F1\",\"Person.identifier.orcid\":\"0000-0003-0301-6182\",\"Person.identifier.researcherID\":\"O-6135-2016\",\"Person.identifier.scopusAuthorID\":\"24167217900\",\"Person.identifier.scopusAuthorID\":\"35447643100\"},{\"Person.name\":\"Podlasiak, Martyna\"},{\"Person.name\":\"Moreira, Manuela M.\",\"Person.identifier.cienciaID\":\"A414-640D-9066\",\"Person.identifier.orcid\":\"0000-0003-1500-9211\",\"Person.identifier.scopusAuthorID\":\"7103289231\"},{\"Person.name\":\"Grosso, Clara\",\"Person.identifier.cienciaID\":\"711B-1170-4C32\",\"Person.identifier.orcid\":\"0000-0002-2457-8267\",\"Person.identifier.researcherID\":\"B-9813-2013\",\"Person.identifier.scopusAuthorID\":\"25627935200\"},{\"Person.name\":\"Rodrigues, Francisca\",\"Person.identifier.cienciaID\":\"4F19-3D98-2CBA\",\"Person.identifier.orcid\":\"0000-0001-8803-0041\"},{\"Person.name\":\"Fernandes, Virgínia Cruz\",\"Person.identifier.cienciaID\":\"5A16-3713-E0CE\",\"Person.identifier.orcid\":\"0000-0003-3979-7523\",\"Person.identifier.researcherID\":\"G-4985-2010\",\"Person.identifier.scopusAuthorID\":\"37045954500\"},{\"Person.name\":\"Delerue-Matos, Cristina\",\"Person.identifier.cienciaID\":\"9A1A-43FB-5C27\",\"Person.identifier.orcid\":\"0000-0002-3924-776X\",\"Person.identifier.researcherID\":\"D-4990-2013\",\"Person.identifier.scopusAuthorID\":\"6603741848\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | REPOSITÓRIO P.PORTO |
| datacite.creators.creator.creatorName.fl_str_mv | Vieira, Elsa F. Podlasiak, Martyna Moreira, Manuela M. Grosso, Clara Rodrigues, Francisca Fernandes, Virgínia Cruz Delerue-Matos, Cristina |
| datacite.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2035-01-01T00:00:00Z |
| datacite.date.embargoed.fl_str_mv | 2035-01-01T00:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Horned Melon fruit Box–Behnken design Maceration Phenolics Neuroprotective activity |
| datacite.titles.title.fl_str_mv | New insights of phytochemical profile and in vitro antioxidant and neuroprotective activities from optimized extract of Horned Melon fruit |
| dc.contributor.none.fl_str_mv | REPOSITÓRIO P.PORTO |
| dc.creator.none.fl_str_mv | Vieira, Elsa F. Podlasiak, Martyna Moreira, Manuela M. Grosso, Clara Rodrigues, Francisca Fernandes, Virgínia Cruz Delerue-Matos, Cristina |
| dc.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2035-01-01T00:00:00Z |
| dc.date.embargoed.fl_str_mv | 2035-01-01T00:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.22/21964 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Springer |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Horned Melon fruit Box–Behnken design Maceration Phenolics Neuroprotective activity |
| dc.title.fl_str_mv | New insights of phytochemical profile and in vitro antioxidant and neuroprotective activities from optimized extract of Horned Melon fruit |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | This study aimed the optimization of antioxidant activity, total phenolic compounds (TPC) and total carotenoids of Horned Melon fruit submitted to maceration extraction, using ethanol as solvent. Optimization was performed by Box–Behnken design on three levels and three variables: extraction temperature (25 °C, 55 °C, and 85 °C), extraction time (0.5, 2.0, and 3.5 h) and ethanol concentration (10%, 50%, and 90%). The optimal conditions to improve the carotenoids extraction was 55 °C, 1.5 h and 86% ethanol, whereas the antiradical/antioxidant activity (ABTS and FRAP assays) and TPC were enhanced at 45 °C for 2.0 h and 40% ethanol. The optimal antioxidant extract was characterized regarding the phenolic composition and in vitro neuroprotective activities. The phenolic composition revealed the presence of high amounts of gallic acid (11.7 ± 0.6 mg/100 g dw) and protocatechuic acid (7.69 ± 0.38 mg/100 g dw). However, this extract presented a weak activity against Monoamine oxidase A (11.8 ± 2.3% inhibition at 2.7 mg/mL) and did not display anti-cholinesterase activity at the maximum concentration tested (1.3 mg/mL). Maceration was an efficient extraction technique to recover polyphenols and carotenoids from Horned Melon fruit. The optimized model can be very useful for food and pharmaceutical industries |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://recipp.ipp.pt/bitstreams/12112b64-66f8-466d-993d-bac2b965c076/download |
| funder_facet_str_mv | FCT{{{_:::_}}}Fundação para a Ciência e a Tecnologia FCT{{{_:::_}}}Fundação para a Ciência e a Tecnologia FCT{{{_:::_}}}Fundação para a Ciência e a Tecnologia FCT{{{_:::_}}}Fundação para a Ciência e a Tecnologia FCT{{{_:::_}}}Fundação para a Ciência e a Tecnologia EC{{{_:::_}}}European Commission |
| funding.funder.alternateName_str_mv | FCT FCT FCT FCT FCT EC |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100008530 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia European Commission |
| funding.identifier_str_mv | UIDB/50006/2020 UIDP/50006/2020 SFRH/BPD/109153/2015 CEECIND/03988/2018/CP1545/CT0006 CEECIND/02702/2017/CP1427/CT0004 696295 |
| funding.name_str_mv | 6817 - DCRRNI ID 6817 - DCRRNI ID POR_NORTE CEEC IND 2018 CEEC IND 2017 H2020 |
| funding_str_mv | UIDB/50006/2020 info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT UIDP/50006/2020 info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50006%2F2020/PT SFRH/BPD/109153/2015 info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBPD%2F109153%2F2015/PT CEECIND/03988/2018/CP1545/CT0006 info:eu-repo/grantAgreement/FCT/CEEC IND 2018/CEECIND%2F03988%2F2018%2FCP1545%2FCT0006/PT CEECIND/02702/2017/CP1427/CT0004 info:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F02702%2F2017%2FCP1427%2FCT0004/PT 696295 info:eu-repo/grantAgreement/EC/H2020/696295/EU |
| id | recipp_0f0e2796dc1b885b2552bab5cd99f553 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.22/21964 |
| inst_facet_str | urn:organizationAcronym:recipp{{{_:::_}}}Instituto Politécnico do Porto |
| instacron_str | recipp |
| institution | Instituto Politécnico do Porto |
| instname_str | Instituto Politécnico do Porto |
| language | eng |
| network_acronym_str | recipp |
| network_name_str | Repositório Científico do Instituto Politécnico do Porto |
| oai_identifier_str | oai:recipp.ipp.pt:10400.22/21964 |
| organization_str_mv | urn:organizationAcronym:recipp |
| person_str_mv | Vieira, Elsa F. Vieira, Elsa F. https://www.ciencia-id.pt/D41C-E7B7-92F1 D41C-E7B7-92F1 http://orcid.org/0000-0003-0301-6182 0000-0003-0301-6182 Podlasiak, Martyna Moreira, Manuela M. Moreira, Manuela M. https://www.ciencia-id.pt/A414-640D-9066 A414-640D-9066 http://orcid.org/0000-0003-1500-9211 0000-0003-1500-9211 Grosso, Clara Grosso, Clara https://www.ciencia-id.pt/711B-1170-4C32 711B-1170-4C32 http://orcid.org/0000-0002-2457-8267 0000-0002-2457-8267 Rodrigues, Francisca Rodrigues, Francisca https://www.ciencia-id.pt/4F19-3D98-2CBA 4F19-3D98-2CBA http://orcid.org/0000-0001-8803-0041 0000-0001-8803-0041 Fernandes, Virgínia Cruz Fernandes, Virgínia Cruz https://www.ciencia-id.pt/5A16-3713-E0CE 5A16-3713-E0CE http://orcid.org/0000-0003-3979-7523 0000-0003-3979-7523 Delerue-Matos, Cristina Delerue-Matos, Cristina https://www.ciencia-id.pt/9A1A-43FB-5C27 9A1A-43FB-5C27 http://orcid.org/0000-0002-3924-776X 0000-0002-3924-776X |
| publishDate | 2022 |
| publisher.none.fl_str_mv | Springer |
| repo_facet_str | urn:repositoryAcronym:recipp{{{_:::_}}}Repositório Científico do Instituto Politécnico do Porto |
| reponame_str | Repositório Científico do Instituto Politécnico do Porto |
| repository_id_str | urn:repositoryAcronym:recipp |
| service_str_mv | urn:repositoryAcronym:recipp |
| spelling | engSpringerpt_PTThis study aimed the optimization of antioxidant activity, total phenolic compounds (TPC) and total carotenoids of Horned Melon fruit submitted to maceration extraction, using ethanol as solvent. Optimization was performed by Box–Behnken design on three levels and three variables: extraction temperature (25 °C, 55 °C, and 85 °C), extraction time (0.5, 2.0, and 3.5 h) and ethanol concentration (10%, 50%, and 90%). The optimal conditions to improve the carotenoids extraction was 55 °C, 1.5 h and 86% ethanol, whereas the antiradical/antioxidant activity (ABTS and FRAP assays) and TPC were enhanced at 45 °C for 2.0 h and 40% ethanol. The optimal antioxidant extract was characterized regarding the phenolic composition and in vitro neuroprotective activities. The phenolic composition revealed the presence of high amounts of gallic acid (11.7 ± 0.6 mg/100 g dw) and protocatechuic acid (7.69 ± 0.38 mg/100 g dw). However, this extract presented a weak activity against Monoamine oxidase A (11.8 ± 2.3% inhibition at 2.7 mg/mL) and did not display anti-cholinesterase activity at the maximum concentration tested (1.3 mg/mL). Maceration was an efficient extraction technique to recover polyphenols and carotenoids from Horned Melon fruit. The optimized model can be very useful for food and pharmaceutical industriesapplication/pdfpt_PTNew insights of phytochemical profile and in vitro antioxidant and neuroprotective activities from optimized extract of Horned Melon fruitPersonalVieira, Elsa F.DSpacehttp://dspace.org/items/33d008c0-bcff-4d0e-8386-a41af9166bb7DSpacehttp://dspace.org/items/33d008c0-bcff-4d0e-8386-a41af9166bb7elsavieiraCiência IDhttps://www.ciencia-id.ptD41C-E7B7-92F1ORCIDhttp://orcid.org0000-0003-0301-6182Researcher IDhttps://www.researcherid.comO-6135-2016Scopus Author IDhttps://www.scopus.com24167217900Scopus Author IDhttps://www.scopus.com35447643100Podlasiak, MartynaPersonalMoreira, Manuela M.DSpacehttp://dspace.org/items/838d4bba-08c3-42be-8e6f-6af2bffcf999DSpacehttp://dspace.org/items/838d4bba-08c3-42be-8e6f-6af2bffcf999MoreiraManuela M.Ciência IDhttps://www.ciencia-id.ptA414-640D-9066ORCIDhttp://orcid.org0000-0003-1500-9211Scopus Author IDhttps://www.scopus.com7103289231PersonalGrosso, ClaraDSpacehttp://dspace.org/items/f8a4443b-2748-4523-8219-691d0523d467DSpacehttp://dspace.org/items/f8a4443b-2748-4523-8219-691d0523d467GrossoAna ClaraCiência IDhttps://www.ciencia-id.pt711B-1170-4C32ORCIDhttp://orcid.org0000-0002-2457-8267Researcher IDhttps://www.researcherid.comB-9813-2013Scopus Author IDhttps://www.scopus.com25627935200PersonalRodrigues, FranciscaDSpacehttp://dspace.org/items/176ed0e9-7da4-4265-bc19-50b694ea58abDSpacehttp://dspace.org/items/176ed0e9-7da4-4265-bc19-50b694ea58abPinto Lisboa Martins Rodrigues SarmentoFranciscaCiência IDhttps://www.ciencia-id.pt4F19-3D98-2CBAORCIDhttp://orcid.org0000-0001-8803-0041PersonalFernandes, Virgínia CruzDSpacehttp://dspace.org/items/e30d74c3-1a3a-4e3e-ada0-5e79362cdd6eDSpacehttp://dspace.org/items/e30d74c3-1a3a-4e3e-ada0-5e79362cdd6eFernandesVirgíniaCiência IDhttps://www.ciencia-id.pt5A16-3713-E0CEORCIDhttp://orcid.org0000-0003-3979-7523Researcher IDhttps://www.researcherid.comG-4985-2010Scopus Author IDhttps://www.scopus.com37045954500PersonalDelerue-Matos, CristinaDSpacehttp://dspace.org/items/09f6a7bd-2f15-42b0-adc5-04bd22210519DSpacehttp://dspace.org/items/09f6a7bd-2f15-42b0-adc5-04bd22210519Delerue-MatosCristinaCiência IDhttps://www.ciencia-id.pt9A1A-43FB-5C27ORCIDhttp://orcid.org0000-0002-3924-776XResearcher IDhttps://www.researcherid.comD-4990-2013Scopus Author IDhttps://www.scopus.com6603741848HostingInstitutionOrganizationalREPOSITÓRIO P.PORTOe-mailmailto:recipp@sc.ipp.ptrecipp@sc.ipp.ptISSNIsPartOf1847–1858DOIIsPartOf10.1007/s11694-022-01307-320222035-01-01T00:00:00Z2022-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.22/21964http://purl.org/coar/access_right/c_16ecrestricted accessHorned Melon fruitBox–Behnken designMacerationPhenolicsNeuroprotective activity2505394 bytesFundação para a Ciência e a TecnologiaAssociated Laboratory for Green Chemistry - Clean Technologies and Processesinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PTUIDB/50006/20206817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaAssociated Laboratory for Green Chemistry - Clean Technologies and Processesinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50006%2F2020/PTUIDP/50006/20206817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaFruitec-Modern analytical technologies for assess Persistent and Emergent Organic Pollutants in red fruitsinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBPD%2F109153%2F2015/PTSFRH/BPD/109153/2015POR_NORTECrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaNot Availableinfo:eu-repo/grantAgreement/FCT/CEEC IND 2018/CEECIND%2F03988%2F2018%2FCP1545%2FCT0006/PTCEECIND/03988/2018/CP1545/CT0006CEEC IND 2018Crossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaNot Availableinfo:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F02702%2F2017%2FCP1427%2FCT0004/PTCEECIND/02702/2017/CP1427/CT0004CEEC IND 2017Crossref Funder IDhttp://doi.org/10.13039/501100001871European CommissionERA-NET Biomarkers for Nutrition and Health implementing the JPI HDHL objectivesinfo:eu-repo/grantAgreement/EC/H2020/696295/EU696295H2020Crossref Funder IDhttp://doi.org/10.13039/501100008530literaturehttp://purl.org/coar/resource_type/c_6501journal article2022http://creativecommons.org/licenses/by-nc-nd/4.0/http://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://recipp.ipp.pt/bitstreams/12112b64-66f8-466d-993d-bac2b965c076/downloadJournal of Food Measurement and Characterization16318471858 |
| spellingShingle | New insights of phytochemical profile and in vitro antioxidant and neuroprotective activities from optimized extract of Horned Melon fruit Vieira, Elsa F. Horned Melon fruit Box–Behnken design Maceration Phenolics Neuroprotective activity |
| status | SINGLETON |
| subject.fl_str_mv | Horned Melon fruit Box–Behnken design Maceration Phenolics Neuroprotective activity |
| title | New insights of phytochemical profile and in vitro antioxidant and neuroprotective activities from optimized extract of Horned Melon fruit |
| title_full | New insights of phytochemical profile and in vitro antioxidant and neuroprotective activities from optimized extract of Horned Melon fruit |
| title_fullStr | New insights of phytochemical profile and in vitro antioxidant and neuroprotective activities from optimized extract of Horned Melon fruit |
| title_full_unstemmed | New insights of phytochemical profile and in vitro antioxidant and neuroprotective activities from optimized extract of Horned Melon fruit |
| title_short | New insights of phytochemical profile and in vitro antioxidant and neuroprotective activities from optimized extract of Horned Melon fruit |
| title_sort | New insights of phytochemical profile and in vitro antioxidant and neuroprotective activities from optimized extract of Horned Melon fruit |
| topic | Horned Melon fruit Box–Behnken design Maceration Phenolics Neuroprotective activity |
| topic_facet | Horned Melon fruit Box–Behnken design Maceration Phenolics Neuroprotective activity |
| url | http://hdl.handle.net/10400.22/21964 |
| visible | 1 |