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Histochemistry and infrared microspectroscopy of lignified tissue in young stems of Struthanthus vulgaris Mart.

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Resumo:In this study, we aimed to determine lignified tissue in young stems of Struthanthus vulgaris Mart. by infrared microspectroscopy and histochemical methods as well as by fluorescence microscopy. Struthanthus vulgaris Mart. is a mistletoe species that belongs to the Loranthaceae family. A brief anatomical description was also carried out. The first procedure for analysis was to elaborate anatomical cross sections (20-30 µm) from young stems before and after treatment with NaOH 1%. This procedure was applied to release possible low molecular mass phenolic compounds. Safranin-astra blue was used to distinguish anatomical tissues while Wiesner test enabled verification of lignified pericyclic fibers. Infrared microspectroscopy analysis confirmed the presence of lignin in this region according to the following spectral signals: 1600 (shoulder), 1511, 1453, 1338 and 1244 cm-1. Analyses of the cross section of young stems under fluorescence microscopy before and after treatment with NaOH 1% allowed us to confirm the presence of low mass phenolic compounds in the region of pericyclic fibers.
Autores principais:Oliveira,Gisely de Lima
Outros Autores:Abreu,Heber dos Santos; Elias,Tatiana de Felice; Silva,Bruno Couto da; Gurgel,Monique de Moura; Gonçalves,Carlos Henrique Rocha; Soares,Geraldo Luiz Gonçalves
Assunto:mistletoe species fibers lignifications
Ano:2013
País:Brasil
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:IBICT
Idioma:inglês
Origem:Oasisbr

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