Publicação
Molecular Identification of Cultivable Bacteria From Infected Root Canals Associated With Acute Apical Abscess
| Resumo: | Abstract The objective of this study was to investigate the bacterial composition present in root canals of teeth associated with acute apical abscess by molecular identification (16S rRNA) of cultivable bacteria. Two hundred and twenty strains isolated by culture from 20 root canals were subjected to DNA extraction and amplification of the 16S rRNA gene (PCR), followed by sequencing. The resulting nucleotide sequences were compared to the GenBank database from the National Center of Biotechnology Information through BLAST. Strains not identified by sequencing were submitted to clonal analysis. The association of microbiological findings with clinical features and the association between microbial species were also investigated. Fifty-nine different cultivable bacteria were identified by 16S rRNA gene sequencing, belonging to 6 phyla, with an average number of 6 species per root canal. Molecular approaches allowed identification of 99% of isolates. The most frequently identified bacteria were Prevotella spp., Pseudoramibacter alactolyticus, Parvimonas micra, Dialister invisus, Filifactor alocis, and Peptostreptococcus stomatis. Positive association was found between Prevotella buccae and Pseudoramibacter alactolyticus and between Parvimonas micra and Prevotella nigrescens (both p<0.05). It was concluded that the microbiota of infected root canals associated with acute apical abscess is diverse and heterogeneous, composed mainly of anaerobic Gram-negative bacteria, with the great majority belonging to the phyla Firmicutes and Bacteroidetes. |
|---|---|
| Autores principais: | Nóbrega,Letícia M. M. |
| Outros Autores: | Montagner,Francisco; Ribeiro,Adriana C.; Mayer,Márcia A. P.; Gomes,Brenda P. F. A. |
| Assunto: | root canals endodontics microorganisms abscess sequencing. |
| Ano: | 2016 |
| País: | Brasil |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | IBICT |
| Idioma: | inglês |
| Origem: | Oasisbr |
| _version_ | 1871509729032798208 |
|---|---|
| author | Nóbrega,Letícia M. M. |
| author2 | Montagner,Francisco Ribeiro,Adriana C. Mayer,Márcia A. P. Gomes,Brenda P. F. A. |
| author2_role | author author author author |
| author_facet | Nóbrega,Letícia M. M. Montagner,Francisco Ribeiro,Adriana C. Mayer,Márcia A. P. Gomes,Brenda P. F. A. |
| author_role | author |
| country_str | BR |
| creators_json_txt | [{\"Person.name\":\"Nóbrega,Letícia M. M.\"},{\"Person.name\":\"Montagner,Francisco\"},{\"Person.name\":\"Ribeiro,Adriana C.\"},{\"Person.name\":\"Mayer,Márcia A. P.\"},{\"Person.name\":\"Gomes,Brenda P. F. A.\"}] |
| datacite.creators.creator.creatorName.fl_str_mv | Nóbrega,Letícia M. M. Montagner,Francisco Ribeiro,Adriana C. Mayer,Márcia A. P. Gomes,Brenda P. F. A. |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | root canals endodontics microorganisms abscess sequencing. |
| datacite.titles.title.fl_str_mv | Molecular Identification of Cultivable Bacteria From Infected Root Canals Associated With Acute Apical Abscess |
| dc.creator.none.fl_str_mv | Nóbrega,Letícia M. M. Montagner,Francisco Ribeiro,Adriana C. Mayer,Márcia A. P. Gomes,Brenda P. F. A. |
| dc.format.none.fl_str_mv | text/html |
| dc.identifier.none.fl_str_mv | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402016000300318 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Fundação Odontológica de Ribeirão Preto |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.source.none.fl_str_mv | Brazilian Dental Journal v.27 n.3 2016 reponame:Brazilian Dental Journal instname:Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
| dc.subject.none.fl_str_mv | root canals endodontics microorganisms abscess sequencing. |
| dc.title.fl_str_mv | Molecular Identification of Cultivable Bacteria From Infected Root Canals Associated With Acute Apical Abscess |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Abstract The objective of this study was to investigate the bacterial composition present in root canals of teeth associated with acute apical abscess by molecular identification (16S rRNA) of cultivable bacteria. Two hundred and twenty strains isolated by culture from 20 root canals were subjected to DNA extraction and amplification of the 16S rRNA gene (PCR), followed by sequencing. The resulting nucleotide sequences were compared to the GenBank database from the National Center of Biotechnology Information through BLAST. Strains not identified by sequencing were submitted to clonal analysis. The association of microbiological findings with clinical features and the association between microbial species were also investigated. Fifty-nine different cultivable bacteria were identified by 16S rRNA gene sequencing, belonging to 6 phyla, with an average number of 6 species per root canal. Molecular approaches allowed identification of 99% of isolates. The most frequently identified bacteria were Prevotella spp., Pseudoramibacter alactolyticus, Parvimonas micra, Dialister invisus, Filifactor alocis, and Peptostreptococcus stomatis. Positive association was found between Prevotella buccae and Pseudoramibacter alactolyticus and between Parvimonas micra and Prevotella nigrescens (both p<0.05). It was concluded that the microbiota of infected root canals associated with acute apical abscess is diverse and heterogeneous, composed mainly of anaerobic Gram-negative bacteria, with the great majority belonging to the phyla Firmicutes and Bacteroidetes. |
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| institution | IBICT |
| instname_str | IBICT |
| language | eng |
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| person_str_mv | Nóbrega,Letícia M. M. Montagner,Francisco Ribeiro,Adriana C. Mayer,Márcia A. P. Gomes,Brenda P. F. A. |
| publishDate | 2016 |
| publisher.none.fl_str_mv | Fundação Odontológica de Ribeirão Preto |
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| spelling | Molecular Identification of Cultivable Bacteria From Infected Root Canals Associated With Acute Apical AbscessNóbrega,Letícia M. M.Montagner,FranciscoRibeiro,Adriana C.Mayer,Márcia A. P.Gomes,Brenda P. F. A.root canalsendodonticsmicroorganismsabscesssequencing.open accesshttp://purl.org/coar/access_right/c_abf2http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402016000300318URLhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402016000300318URLHasVersion2016-06-01Abstract The objective of this study was to investigate the bacterial composition present in root canals of teeth associated with acute apical abscess by molecular identification (16S rRNA) of cultivable bacteria. Two hundred and twenty strains isolated by culture from 20 root canals were subjected to DNA extraction and amplification of the 16S rRNA gene (PCR), followed by sequencing. The resulting nucleotide sequences were compared to the GenBank database from the National Center of Biotechnology Information through BLAST. Strains not identified by sequencing were submitted to clonal analysis. The association of microbiological findings with clinical features and the association between microbial species were also investigated. Fifty-nine different cultivable bacteria were identified by 16S rRNA gene sequencing, belonging to 6 phyla, with an average number of 6 species per root canal. Molecular approaches allowed identification of 99% of isolates. The most frequently identified bacteria were Prevotella spp., Pseudoramibacter alactolyticus, Parvimonas micra, Dialister invisus, Filifactor alocis, and Peptostreptococcus stomatis. Positive association was found between Prevotella buccae and Pseudoramibacter alactolyticus and between Parvimonas micra and Prevotella nigrescens (both p<0.05). It was concluded that the microbiota of infected root canals associated with acute apical abscess is diverse and heterogeneous, composed mainly of anaerobic Gram-negative bacteria, with the great majority belonging to the phyla Firmicutes and Bacteroidetes.engtext/htmlFundação Odontológica de Ribeirão PretoBrazilian Dental Journal v.27 n.3 2016reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORPjournal articlehttp://purl.org/coar/resource_type/c_6501literatureVoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85 |
| spellingShingle | Molecular Identification of Cultivable Bacteria From Infected Root Canals Associated With Acute Apical Abscess Nóbrega,Letícia M. M. root canals endodontics microorganisms abscess sequencing. |
| status | SINGLETON |
| status_str | VoR |
| subject.fl_str_mv | root canals endodontics microorganisms abscess sequencing. |
| title | Molecular Identification of Cultivable Bacteria From Infected Root Canals Associated With Acute Apical Abscess |
| title_full | Molecular Identification of Cultivable Bacteria From Infected Root Canals Associated With Acute Apical Abscess |
| title_fullStr | Molecular Identification of Cultivable Bacteria From Infected Root Canals Associated With Acute Apical Abscess |
| title_full_unstemmed | Molecular Identification of Cultivable Bacteria From Infected Root Canals Associated With Acute Apical Abscess |
| title_short | Molecular Identification of Cultivable Bacteria From Infected Root Canals Associated With Acute Apical Abscess |
| title_sort | Molecular Identification of Cultivable Bacteria From Infected Root Canals Associated With Acute Apical Abscess |
| topic | root canals endodontics microorganisms abscess sequencing. |
| topic_facet | root canals endodontics microorganisms abscess sequencing. |
| url | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402016000300318 |
| visible | 1 |
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