Publicação
Biosensors of laccase based on hydrophobic ionic liquids derived from imidazolium cation
| Resumo: | Biosensors based on laccase from Aspergillus oryzae and ionic liquids derived from the 1-butyl-3-methylimidazolium cation (BMI) associated with the anions hexafluorophosphate (BMI·PF6) or bis(trifluoromethylsulfonyl)imide (BMI·Tf2N) were constructed for adrenaline determination. The biosensor based on BMI·Tf2N was selected by presenting higher response when compared with that based on BMI·PF6. The best conditions for the optimization were established by square wave voltammetry (pulse amplitude 100 mV, frequency 10 Hz and scan increment 4.0 mV). The best performance was obtained with 50:20:15:15% (m/m/m/m) as the graphite powder:laccase:Nujol:ILs composition in 0.1 mol L-1 acetate buffer solution (pH 4.0). The analytical curve was linear in the concentration range 2.49 × 10-6 to 2.27 × 10-4 mol L-1 with a detection limit of 5.34 × 10-7 mol L-1. The recovery of adrenaline in injectable samples ranged from 96.3 to 101.6%. The results obtained for the adrenaline using the proposed biosensor and the United States Pharmacopeia procedure were in agreement at the 95% confidence level. |
|---|---|
| Autores principais: | Franzoi,Ana Cristina |
| Outros Autores: | Vieira,Iolanda Cruz; Dupont,Jairton |
| Assunto: | biosensor laccase ionic liquids imidazolium bis(trifluoromethylsulfonyl)imide adrenaline |
| Ano: | 2010 |
| País: | Brasil |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | IBICT |
| Idioma: | inglês |
| Origem: | Oasisbr |
| _version_ | 1871506604959989760 |
|---|---|
| author | Franzoi,Ana Cristina |
| author2 | Vieira,Iolanda Cruz Dupont,Jairton |
| author2_role | author author |
| author_facet | Franzoi,Ana Cristina Vieira,Iolanda Cruz Dupont,Jairton |
| author_role | author |
| country_str | BR |
| creators_json_txt | [{\"Person.name\":\"Franzoi,Ana Cristina\"},{\"Person.name\":\"Vieira,Iolanda Cruz\"},{\"Person.name\":\"Dupont,Jairton\"}] |
| datacite.creators.creator.creatorName.fl_str_mv | Franzoi,Ana Cristina Vieira,Iolanda Cruz Dupont,Jairton |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | biosensor laccase ionic liquids imidazolium bis(trifluoromethylsulfonyl)imide adrenaline |
| datacite.titles.title.fl_str_mv | Biosensors of laccase based on hydrophobic ionic liquids derived from imidazolium cation |
| dc.creator.none.fl_str_mv | Franzoi,Ana Cristina Vieira,Iolanda Cruz Dupont,Jairton |
| dc.format.none.fl_str_mv | text/html |
| dc.identifier.none.fl_str_mv | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532010000800007 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Sociedade Brasileira de Química |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.source.none.fl_str_mv | Journal of the Brazilian Chemical Society v.21 n.8 2010 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
| dc.subject.none.fl_str_mv | biosensor laccase ionic liquids imidazolium bis(trifluoromethylsulfonyl)imide adrenaline |
| dc.title.fl_str_mv | Biosensors of laccase based on hydrophobic ionic liquids derived from imidazolium cation |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Biosensors based on laccase from Aspergillus oryzae and ionic liquids derived from the 1-butyl-3-methylimidazolium cation (BMI) associated with the anions hexafluorophosphate (BMI·PF6) or bis(trifluoromethylsulfonyl)imide (BMI·Tf2N) were constructed for adrenaline determination. The biosensor based on BMI·Tf2N was selected by presenting higher response when compared with that based on BMI·PF6. The best conditions for the optimization were established by square wave voltammetry (pulse amplitude 100 mV, frequency 10 Hz and scan increment 4.0 mV). The best performance was obtained with 50:20:15:15% (m/m/m/m) as the graphite powder:laccase:Nujol:ILs composition in 0.1 mol L-1 acetate buffer solution (pH 4.0). The analytical curve was linear in the concentration range 2.49 × 10-6 to 2.27 × 10-4 mol L-1 with a detection limit of 5.34 × 10-7 mol L-1. The recovery of adrenaline in injectable samples ranged from 96.3 to 101.6%. The results obtained for the adrenaline using the proposed biosensor and the United States Pharmacopeia procedure were in agreement at the 95% confidence level. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| id | oasisbr_c7e611b12315209fac1909b43b5358e6 |
| identifier.url.fl_str_mv | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532010000800007 |
| inst_facet_str | urn:organizationAcronym:oasisbr{{{_:::_}}}IBICT |
| instacron_str | OASISBR |
| institution | IBICT |
| instname_str | IBICT |
| language | eng |
| network_acronym_str | oasisbr |
| network_name_str | Oasisbr |
| oai_identifier_str | oai:scielo:S0103-50532010000800007 |
| organization_str_mv | urn:organizationAcronym:oasisbr |
| person_str_mv | Franzoi,Ana Cristina Vieira,Iolanda Cruz Dupont,Jairton |
| publishDate | 2010 |
| publisher.none.fl_str_mv | Sociedade Brasileira de Química |
| repo_facet_str | urn:repositoryAcronym:oasisbr{{{_:::_}}}Oasisbr |
| reponame_str | Oasisbr |
| repository_id_str | urn:repositoryAcronym:oasisbr |
| service_str_mv | urn:repositoryAcronym:oasisbr |
| spelling | Biosensors of laccase based on hydrophobic ionic liquids derived from imidazolium cationFranzoi,Ana CristinaVieira,Iolanda CruzDupont,Jairtonbiosensorlaccaseionic liquidsimidazoliumbis(trifluoromethylsulfonyl)imideadrenalineopen accesshttp://purl.org/coar/access_right/c_abf2http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532010000800007URLhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532010000800007URLHasVersion2010-01-01Biosensors based on laccase from Aspergillus oryzae and ionic liquids derived from the 1-butyl-3-methylimidazolium cation (BMI) associated with the anions hexafluorophosphate (BMI·PF6) or bis(trifluoromethylsulfonyl)imide (BMI·Tf2N) were constructed for adrenaline determination. The biosensor based on BMI·Tf2N was selected by presenting higher response when compared with that based on BMI·PF6. The best conditions for the optimization were established by square wave voltammetry (pulse amplitude 100 mV, frequency 10 Hz and scan increment 4.0 mV). The best performance was obtained with 50:20:15:15% (m/m/m/m) as the graphite powder:laccase:Nujol:ILs composition in 0.1 mol L-1 acetate buffer solution (pH 4.0). The analytical curve was linear in the concentration range 2.49 × 10-6 to 2.27 × 10-4 mol L-1 with a detection limit of 5.34 × 10-7 mol L-1. The recovery of adrenaline in injectable samples ranged from 96.3 to 101.6%. The results obtained for the adrenaline using the proposed biosensor and the United States Pharmacopeia procedure were in agreement at the 95% confidence level.engtext/htmlSociedade Brasileira de QuímicaJournal of the Brazilian Chemical Society v.21 n.8 2010reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQjournal articlehttp://purl.org/coar/resource_type/c_6501literatureVoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85 |
| spellingShingle | Biosensors of laccase based on hydrophobic ionic liquids derived from imidazolium cation Franzoi,Ana Cristina biosensor laccase ionic liquids imidazolium bis(trifluoromethylsulfonyl)imide adrenaline |
| status | SINGLETON |
| status_str | VoR |
| subject.fl_str_mv | biosensor laccase ionic liquids imidazolium bis(trifluoromethylsulfonyl)imide adrenaline |
| title | Biosensors of laccase based on hydrophobic ionic liquids derived from imidazolium cation |
| title_full | Biosensors of laccase based on hydrophobic ionic liquids derived from imidazolium cation |
| title_fullStr | Biosensors of laccase based on hydrophobic ionic liquids derived from imidazolium cation |
| title_full_unstemmed | Biosensors of laccase based on hydrophobic ionic liquids derived from imidazolium cation |
| title_short | Biosensors of laccase based on hydrophobic ionic liquids derived from imidazolium cation |
| title_sort | Biosensors of laccase based on hydrophobic ionic liquids derived from imidazolium cation |
| topic | biosensor laccase ionic liquids imidazolium bis(trifluoromethylsulfonyl)imide adrenaline |
| topic_facet | biosensor laccase ionic liquids imidazolium bis(trifluoromethylsulfonyl)imide adrenaline |
| url | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532010000800007 |
| visible | 1 |
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