Publicação
Isolation and 2,4-D-degrading characteristics of Cupriavidus campinensis BJ71
| Resumo: | An indigenous bacterial strain capable of utilizing 2,4-dichlorophenoxyacetic acid as the sole carbon and energy source was isolated from a soil used for grown wheat with a long-term history of herbicide use in Beijing, China. The strain BJ71 was identified as Cupriavidus campinensis based on its 16S rRNA sequence analysis and morphological, physiological, and biochemical characteristics. The degradation characteristics of strain BJ71 were evaluated. The optimal conditions for 2,4-D degradation were as follows: pH 7.0, 30 °C, 3% (v/v) inoculum size, and an initial 2,4-D concentration of 350 mg L−1. Up to 99.57% of the 2,4-D was degraded under optimal conditions after 6 days of incubation. Strain BJ71 was also able to degrade quizalofop and fluroxypyr. This is the first report of a 2,4-D-degrader containing tfdA gene that can utilize these two herbicides. In a biodegradation experiment, 87.13% and 42.53% of 2,4-D (initial concentration, 350 mg kg−1) was degraded in non-sterile and sterilized soil inoculated with BJ71, respectively, after 14 days. The 2,4-D degradation was more rapid in a soil microcosm including BJ71 than in a soil microcosm without BJ71. These results indicate that strain BJ71 is a potential candidate for the bioremediation of soil contaminated with the herbicide 2,4-D. |
|---|---|
| Autores principais: | Han,Lizhen |
| Outros Autores: | Zhao,Degang; Li,Cuicui |
| Assunto: | 2,4-D-degrading bacterial strain bioremediation Cupriavidus campinensis BJ71 degradation characteristics |
| Ano: | 2015 |
| País: | Brasil |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | IBICT |
| Idioma: | inglês |
| Origem: | Oasisbr |
| _version_ | 1871507111577387008 |
|---|---|
| author | Han,Lizhen |
| author2 | Zhao,Degang Li,Cuicui |
| author2_role | author author |
| author_facet | Han,Lizhen Zhao,Degang Li,Cuicui |
| author_role | author |
| country_str | BR |
| creators_json_txt | [{\"Person.name\":\"Han,Lizhen\"},{\"Person.name\":\"Zhao,Degang\"},{\"Person.name\":\"Li,Cuicui\"}] |
| datacite.creators.creator.creatorName.fl_str_mv | Han,Lizhen Zhao,Degang Li,Cuicui |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | 2,4-D-degrading bacterial strain bioremediation Cupriavidus campinensis BJ71 degradation characteristics |
| datacite.titles.title.fl_str_mv | Isolation and 2,4-D-degrading characteristics of Cupriavidus campinensis BJ71 |
| dc.creator.none.fl_str_mv | Han,Lizhen Zhao,Degang Li,Cuicui |
| dc.format.none.fl_str_mv | text/html |
| dc.identifier.none.fl_str_mv | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200433 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Sociedade Brasileira de Microbiologia |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.source.none.fl_str_mv | Brazilian Journal of Microbiology v.46 n.2 2015 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
| dc.subject.none.fl_str_mv | 2,4-D-degrading bacterial strain bioremediation Cupriavidus campinensis BJ71 degradation characteristics |
| dc.title.fl_str_mv | Isolation and 2,4-D-degrading characteristics of Cupriavidus campinensis BJ71 |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | An indigenous bacterial strain capable of utilizing 2,4-dichlorophenoxyacetic acid as the sole carbon and energy source was isolated from a soil used for grown wheat with a long-term history of herbicide use in Beijing, China. The strain BJ71 was identified as Cupriavidus campinensis based on its 16S rRNA sequence analysis and morphological, physiological, and biochemical characteristics. The degradation characteristics of strain BJ71 were evaluated. The optimal conditions for 2,4-D degradation were as follows: pH 7.0, 30 °C, 3% (v/v) inoculum size, and an initial 2,4-D concentration of 350 mg L−1. Up to 99.57% of the 2,4-D was degraded under optimal conditions after 6 days of incubation. Strain BJ71 was also able to degrade quizalofop and fluroxypyr. This is the first report of a 2,4-D-degrader containing tfdA gene that can utilize these two herbicides. In a biodegradation experiment, 87.13% and 42.53% of 2,4-D (initial concentration, 350 mg kg−1) was degraded in non-sterile and sterilized soil inoculated with BJ71, respectively, after 14 days. The 2,4-D degradation was more rapid in a soil microcosm including BJ71 than in a soil microcosm without BJ71. These results indicate that strain BJ71 is a potential candidate for the bioremediation of soil contaminated with the herbicide 2,4-D. |
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| eu_rights_str_mv | openAccess |
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| identifier.url.fl_str_mv | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200433 |
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| language | eng |
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| person_str_mv | Han,Lizhen Zhao,Degang Li,Cuicui |
| publishDate | 2015 |
| publisher.none.fl_str_mv | Sociedade Brasileira de Microbiologia |
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| spelling | Isolation and 2,4-D-degrading characteristics of Cupriavidus campinensis BJ71Han,LizhenZhao,DegangLi,Cuicui2,4-D-degrading bacterial strainbioremediationCupriavidus campinensis BJ71degradation characteristicsopen accesshttp://purl.org/coar/access_right/c_abf2http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200433URLhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200433URLHasVersion2015-06-01An indigenous bacterial strain capable of utilizing 2,4-dichlorophenoxyacetic acid as the sole carbon and energy source was isolated from a soil used for grown wheat with a long-term history of herbicide use in Beijing, China. The strain BJ71 was identified as Cupriavidus campinensis based on its 16S rRNA sequence analysis and morphological, physiological, and biochemical characteristics. The degradation characteristics of strain BJ71 were evaluated. The optimal conditions for 2,4-D degradation were as follows: pH 7.0, 30 °C, 3% (v/v) inoculum size, and an initial 2,4-D concentration of 350 mg L−1. Up to 99.57% of the 2,4-D was degraded under optimal conditions after 6 days of incubation. Strain BJ71 was also able to degrade quizalofop and fluroxypyr. This is the first report of a 2,4-D-degrader containing tfdA gene that can utilize these two herbicides. In a biodegradation experiment, 87.13% and 42.53% of 2,4-D (initial concentration, 350 mg kg−1) was degraded in non-sterile and sterilized soil inoculated with BJ71, respectively, after 14 days. The 2,4-D degradation was more rapid in a soil microcosm including BJ71 than in a soil microcosm without BJ71. These results indicate that strain BJ71 is a potential candidate for the bioremediation of soil contaminated with the herbicide 2,4-D.engtext/htmlSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology v.46 n.2 2015reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBMjournal articlehttp://purl.org/coar/resource_type/c_6501literatureVoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85 |
| spellingShingle | Isolation and 2,4-D-degrading characteristics of Cupriavidus campinensis BJ71 Han,Lizhen 2,4-D-degrading bacterial strain bioremediation Cupriavidus campinensis BJ71 degradation characteristics |
| status | SINGLETON |
| status_str | VoR |
| subject.fl_str_mv | 2,4-D-degrading bacterial strain bioremediation Cupriavidus campinensis BJ71 degradation characteristics |
| title | Isolation and 2,4-D-degrading characteristics of Cupriavidus campinensis BJ71 |
| title_full | Isolation and 2,4-D-degrading characteristics of Cupriavidus campinensis BJ71 |
| title_fullStr | Isolation and 2,4-D-degrading characteristics of Cupriavidus campinensis BJ71 |
| title_full_unstemmed | Isolation and 2,4-D-degrading characteristics of Cupriavidus campinensis BJ71 |
| title_short | Isolation and 2,4-D-degrading characteristics of Cupriavidus campinensis BJ71 |
| title_sort | Isolation and 2,4-D-degrading characteristics of Cupriavidus campinensis BJ71 |
| topic | 2,4-D-degrading bacterial strain bioremediation Cupriavidus campinensis BJ71 degradation characteristics |
| topic_facet | 2,4-D-degrading bacterial strain bioremediation Cupriavidus campinensis BJ71 degradation characteristics |
| url | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200433 |
| visible | 1 |
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