Publicação
Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center
| Resumo: | ABSTRACT Purpose: To analyze the presence of microorganisms in fluorescein eyedrops used in a reference eye center in Recife-PE. Methods: This real-life and masked study evaluated fluorescein eyedrops used at the Altino Ventura Foundation in May 2019. Cultures were performed according to exposure times; I) three eyedrop bottles were analyzed after one day of use, II) three eyedrop bottles after 4 d of use, III) three eyedrop bottles after 8 d of use, and IV) three unopened bottles used as control. Samples were collected from the bottle's tip, instilled drop, and residual fluid. After incubation, all colonies were analyzed and identified through biochemical tests. Results: The contamination rate of the fluorescein eyedrop bottles in this study was 55.5% (5/9 vials). There was no contamination in the control group. The highest contamination was seen in one day exposed eyedrops, in 100% of the bottles. The bottle's tip had a higher rate of contamination compared to the drop and residual fluid. Gram-positive bacteria were isolated in 7/27 (25.9%) samples. Growth of fungi or gram-negative bacteria was not observed. Conclusion: The identification of gram-positive bacteria predominantly on the tip of the fluorescein eyedrop bottles suggests inadequate handling as the main cause of contamination. |
|---|---|
| Autores principais: | Costa,Camila de Moraes |
| Outros Autores: | Espósito,Kayo Pinheiro; Florêncio,Victor Medeiros Brandão; Aquino,Paulo Romero Leite; Firmo,Elza Ferreira; Costa,Iggor Macêdo do Amaral; Rocha,Camilla; Andrade,Camila Galvão de; Oliveira-Júnior,Jorge Belém; Ventura,Camila V. |
| Assunto: | Fluorescein Ophthalmic solution Drug contamination Eye infection, bacterial/microbiology Bacteria/isolation & purification |
| Ano: | 2021 |
| País: | Brasil |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | IBICT |
| Idioma: | inglês |
| Origem: | Oasisbr |
| _version_ | 1871509981786800128 |
|---|---|
| author | Costa,Camila de Moraes |
| author2 | Espósito,Kayo Pinheiro Florêncio,Victor Medeiros Brandão Aquino,Paulo Romero Leite Firmo,Elza Ferreira Costa,Iggor Macêdo do Amaral Rocha,Camilla Andrade,Camila Galvão de Oliveira-Júnior,Jorge Belém Ventura,Camila V. |
| author2_role | author author author author author author author author author |
| author_facet | Costa,Camila de Moraes Espósito,Kayo Pinheiro Florêncio,Victor Medeiros Brandão Aquino,Paulo Romero Leite Firmo,Elza Ferreira Costa,Iggor Macêdo do Amaral Rocha,Camilla Andrade,Camila Galvão de Oliveira-Júnior,Jorge Belém Ventura,Camila V. |
| author_role | author |
| country_str | BR |
| creators_json_txt | [{\"Person.name\":\"Costa,Camila de Moraes\"},{\"Person.name\":\"Espósito,Kayo Pinheiro\"},{\"Person.name\":\"Florêncio,Victor Medeiros Brandão\"},{\"Person.name\":\"Aquino,Paulo Romero Leite\"},{\"Person.name\":\"Firmo,Elza Ferreira\"},{\"Person.name\":\"Costa,Iggor Macêdo do Amaral\"},{\"Person.name\":\"Rocha,Camilla\"},{\"Person.name\":\"Andrade,Camila Galvão de\"},{\"Person.name\":\"Oliveira-Júnior,Jorge Belém\"},{\"Person.name\":\"Ventura,Camila V.\"}] |
| datacite.creators.creator.creatorName.fl_str_mv | Costa,Camila de Moraes Espósito,Kayo Pinheiro Florêncio,Victor Medeiros Brandão Aquino,Paulo Romero Leite Firmo,Elza Ferreira Costa,Iggor Macêdo do Amaral Rocha,Camilla Andrade,Camila Galvão de Oliveira-Júnior,Jorge Belém Ventura,Camila V. |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Fluorescein Ophthalmic solution Drug contamination Eye infection, bacterial/microbiology Bacteria/isolation & purification |
| datacite.titles.title.fl_str_mv | Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center |
| dc.creator.none.fl_str_mv | Costa,Camila de Moraes Espósito,Kayo Pinheiro Florêncio,Victor Medeiros Brandão Aquino,Paulo Romero Leite Firmo,Elza Ferreira Costa,Iggor Macêdo do Amaral Rocha,Camilla Andrade,Camila Galvão de Oliveira-Júnior,Jorge Belém Ventura,Camila V. |
| dc.format.none.fl_str_mv | text/html |
| dc.identifier.none.fl_str_mv | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492021005007201 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Conselho Brasileiro de Oftalmologia |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.source.none.fl_str_mv | Arquivos Brasileiros de Oftalmologia n.ahead 2021 reponame:Arquivos brasileiros de oftalmologia (Online) instname:Conselho Brasileiro de Oftalmologia (CBO) instacron:CBO |
| dc.subject.none.fl_str_mv | Fluorescein Ophthalmic solution Drug contamination Eye infection, bacterial/microbiology Bacteria/isolation & purification |
| dc.title.fl_str_mv | Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | ABSTRACT Purpose: To analyze the presence of microorganisms in fluorescein eyedrops used in a reference eye center in Recife-PE. Methods: This real-life and masked study evaluated fluorescein eyedrops used at the Altino Ventura Foundation in May 2019. Cultures were performed according to exposure times; I) three eyedrop bottles were analyzed after one day of use, II) three eyedrop bottles after 4 d of use, III) three eyedrop bottles after 8 d of use, and IV) three unopened bottles used as control. Samples were collected from the bottle's tip, instilled drop, and residual fluid. After incubation, all colonies were analyzed and identified through biochemical tests. Results: The contamination rate of the fluorescein eyedrop bottles in this study was 55.5% (5/9 vials). There was no contamination in the control group. The highest contamination was seen in one day exposed eyedrops, in 100% of the bottles. The bottle's tip had a higher rate of contamination compared to the drop and residual fluid. Gram-positive bacteria were isolated in 7/27 (25.9%) samples. Growth of fungi or gram-negative bacteria was not observed. Conclusion: The identification of gram-positive bacteria predominantly on the tip of the fluorescein eyedrop bottles suggests inadequate handling as the main cause of contamination. |
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| eu_rights_str_mv | openAccess |
| format | article |
| id | oasisbr_aec385c7c190445621ae75a0807ddffc |
| identifier.url.fl_str_mv | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492021005007201 |
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| instacron_str | OASISBR |
| institution | IBICT |
| instname_str | IBICT |
| language | eng |
| network_acronym_str | oasisbr |
| network_name_str | Oasisbr |
| oai_identifier_str | oai:scielo:S0004-27492021005007201 |
| organization_str_mv | urn:organizationAcronym:oasisbr |
| person_str_mv | Costa,Camila de Moraes Espósito,Kayo Pinheiro Florêncio,Victor Medeiros Brandão Aquino,Paulo Romero Leite Firmo,Elza Ferreira Costa,Iggor Macêdo do Amaral Rocha,Camilla Andrade,Camila Galvão de Oliveira-Júnior,Jorge Belém Ventura,Camila V. |
| publishDate | 2021 |
| publisher.none.fl_str_mv | Conselho Brasileiro de Oftalmologia |
| repo_facet_str | urn:repositoryAcronym:oasisbr{{{_:::_}}}Oasisbr |
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| spelling | Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye centerCosta,Camila de MoraesEspósito,Kayo PinheiroFlorêncio,Victor Medeiros BrandãoAquino,Paulo Romero LeiteFirmo,Elza FerreiraCosta,Iggor Macêdo do AmaralRocha,CamillaAndrade,Camila Galvão deOliveira-Júnior,Jorge BelémVentura,Camila V.FluoresceinOphthalmic solutionDrug contaminationEye infection, bacterial/microbiologyBacteria/isolation & purificationopen accesshttp://purl.org/coar/access_right/c_abf2http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492021005007201URLhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492021005007201URLHasVersion2021-01-01ABSTRACT Purpose: To analyze the presence of microorganisms in fluorescein eyedrops used in a reference eye center in Recife-PE. Methods: This real-life and masked study evaluated fluorescein eyedrops used at the Altino Ventura Foundation in May 2019. Cultures were performed according to exposure times; I) three eyedrop bottles were analyzed after one day of use, II) three eyedrop bottles after 4 d of use, III) three eyedrop bottles after 8 d of use, and IV) three unopened bottles used as control. Samples were collected from the bottle's tip, instilled drop, and residual fluid. After incubation, all colonies were analyzed and identified through biochemical tests. Results: The contamination rate of the fluorescein eyedrop bottles in this study was 55.5% (5/9 vials). There was no contamination in the control group. The highest contamination was seen in one day exposed eyedrops, in 100% of the bottles. The bottle's tip had a higher rate of contamination compared to the drop and residual fluid. Gram-positive bacteria were isolated in 7/27 (25.9%) samples. Growth of fungi or gram-negative bacteria was not observed. Conclusion: The identification of gram-positive bacteria predominantly on the tip of the fluorescein eyedrop bottles suggests inadequate handling as the main cause of contamination.engtext/htmlConselho Brasileiro de OftalmologiaArquivos Brasileiros de Oftalmologia n.ahead 2021reponame:Arquivos brasileiros de oftalmologia (Online)instname:Conselho Brasileiro de Oftalmologia (CBO)instacron:CBOjournal articlehttp://purl.org/coar/resource_type/c_6501literatureVoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85 |
| spellingShingle | Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center Costa,Camila de Moraes Fluorescein Ophthalmic solution Drug contamination Eye infection, bacterial/microbiology Bacteria/isolation & purification |
| status | SINGLETON |
| status_str | VoR |
| subject.fl_str_mv | Fluorescein Ophthalmic solution Drug contamination Eye infection, bacterial/microbiology Bacteria/isolation & purification |
| title | Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center |
| title_full | Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center |
| title_fullStr | Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center |
| title_full_unstemmed | Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center |
| title_short | Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center |
| title_sort | Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center |
| topic | Fluorescein Ophthalmic solution Drug contamination Eye infection, bacterial/microbiology Bacteria/isolation & purification |
| topic_facet | Fluorescein Ophthalmic solution Drug contamination Eye infection, bacterial/microbiology Bacteria/isolation & purification |
| url | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492021005007201 |
| visible | 1 |
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