Publicação
Catecholamines production by kidney tissue and mesangial cell culture is differentially modulated by diabetes
| Resumo: | Abstract Introduction: According to the International Diabetes Federation, the number of people with diabetes mellitus may reach 700 million in 2045. Catecholamines are involved in the regulation of several kidney functions. This study investigates the effects of hyperglycemia on catecholamines' metabolism in kidney tissue from control, diabetic, and insulin-treated diabetic rats, both in vivo and in vitro. Methods: Male Wistar-Hannover rats were randomized into: control, diabetic, and insulin-treated diabetic groups. Diabetes was induced by a single injection of streptozotocin, and diabetic treated group also received insulin. After 60 days, blood and kidney tissue from all groups were collected for catecholamines' quantification and mesangial cells culture. Results: diabetic rats had lower body weight, hyperglycemia, and increase water intake and diuresis. Additionally, diabetes promoted a sharp decrease in creatinine clearance compared to control group. Regarding the whole kidney extracts, both diabetic groups (treated and non-treated) had significant reduction in norepinephrine concentration. In mesangial cell culture, catecholamines' concentration were lower in the culture medium than in the intracellular compartment for all groups. Norepinephrine, epinephrine, and dopamine medium levels were increased in the diabetic group. Conclusion: The major finding of the present study was that 8 weeks of diabetes induction altered the kidney catecholaminergic system in a very specific manner, once the production of catecholamines in the excised kidney tissue from diabetic rats was differentially modulated as compared with the production and secretion by cultured mesangial cells. |
|---|---|
| Autores principais: | Moreira,Roseli Peres |
| Outros Autores: | Bertoncello,Nadia S. C.; Colucci,Juliana Almada; Arita,Danielle Yuri; Andrade,Maria Claudina Camargo de; Ronchi,Fernanda Aparecida; Cunha,Tatiana Sousa; Casarini,Dulce Elena |
| Assunto: | Diabetes Mellitus Catecholamines Norepinephrine Epinephrine Dopamine |
| Ano: | 2021 |
| País: | Brasil |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | IBICT |
| Idioma: | inglês |
| Origem: | Oasisbr |
| _version_ | 1871506973907746816 |
|---|---|
| author | Moreira,Roseli Peres |
| author2 | Bertoncello,Nadia S. C. Colucci,Juliana Almada Arita,Danielle Yuri Andrade,Maria Claudina Camargo de Ronchi,Fernanda Aparecida Cunha,Tatiana Sousa Casarini,Dulce Elena |
| author2_role | author author author author author author author |
| author_facet | Moreira,Roseli Peres Bertoncello,Nadia S. C. Colucci,Juliana Almada Arita,Danielle Yuri Andrade,Maria Claudina Camargo de Ronchi,Fernanda Aparecida Cunha,Tatiana Sousa Casarini,Dulce Elena |
| author_role | author |
| country_str | BR |
| creators_json_txt | [{\"Person.name\":\"Moreira,Roseli Peres\"},{\"Person.name\":\"Bertoncello,Nadia S. C.\"},{\"Person.name\":\"Colucci,Juliana Almada\"},{\"Person.name\":\"Arita,Danielle Yuri\"},{\"Person.name\":\"Andrade,Maria Claudina Camargo de\"},{\"Person.name\":\"Ronchi,Fernanda Aparecida\"},{\"Person.name\":\"Cunha,Tatiana Sousa\"},{\"Person.name\":\"Casarini,Dulce Elena\"}] |
| datacite.creators.creator.creatorName.fl_str_mv | Moreira,Roseli Peres Bertoncello,Nadia S. C. Colucci,Juliana Almada Arita,Danielle Yuri Andrade,Maria Claudina Camargo de Ronchi,Fernanda Aparecida Cunha,Tatiana Sousa Casarini,Dulce Elena |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Diabetes Mellitus Catecholamines Norepinephrine Epinephrine Dopamine |
| datacite.titles.title.fl_str_mv | Catecholamines production by kidney tissue and mesangial cell culture is differentially modulated by diabetes |
| dc.creator.none.fl_str_mv | Moreira,Roseli Peres Bertoncello,Nadia S. C. Colucci,Juliana Almada Arita,Danielle Yuri Andrade,Maria Claudina Camargo de Ronchi,Fernanda Aparecida Cunha,Tatiana Sousa Casarini,Dulce Elena |
| dc.format.none.fl_str_mv | text/html |
| dc.identifier.none.fl_str_mv | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-28002021000400510 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Sociedade Brasileira de Nefrologia |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.source.none.fl_str_mv | Brazilian Journal of Nephrology v.43 n.4 2021 reponame:Jornal Brasileiro de Nefrologia instname:Sociedade Brasileira de Nefrologia (SBN) instacron:SBN |
| dc.subject.none.fl_str_mv | Diabetes Mellitus Catecholamines Norepinephrine Epinephrine Dopamine |
| dc.title.fl_str_mv | Catecholamines production by kidney tissue and mesangial cell culture is differentially modulated by diabetes |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Abstract Introduction: According to the International Diabetes Federation, the number of people with diabetes mellitus may reach 700 million in 2045. Catecholamines are involved in the regulation of several kidney functions. This study investigates the effects of hyperglycemia on catecholamines' metabolism in kidney tissue from control, diabetic, and insulin-treated diabetic rats, both in vivo and in vitro. Methods: Male Wistar-Hannover rats were randomized into: control, diabetic, and insulin-treated diabetic groups. Diabetes was induced by a single injection of streptozotocin, and diabetic treated group also received insulin. After 60 days, blood and kidney tissue from all groups were collected for catecholamines' quantification and mesangial cells culture. Results: diabetic rats had lower body weight, hyperglycemia, and increase water intake and diuresis. Additionally, diabetes promoted a sharp decrease in creatinine clearance compared to control group. Regarding the whole kidney extracts, both diabetic groups (treated and non-treated) had significant reduction in norepinephrine concentration. In mesangial cell culture, catecholamines' concentration were lower in the culture medium than in the intracellular compartment for all groups. Norepinephrine, epinephrine, and dopamine medium levels were increased in the diabetic group. Conclusion: The major finding of the present study was that 8 weeks of diabetes induction altered the kidney catecholaminergic system in a very specific manner, once the production of catecholamines in the excised kidney tissue from diabetic rats was differentially modulated as compared with the production and secretion by cultured mesangial cells. |
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| person_str_mv | Moreira,Roseli Peres Bertoncello,Nadia S. C. Colucci,Juliana Almada Arita,Danielle Yuri Andrade,Maria Claudina Camargo de Ronchi,Fernanda Aparecida Cunha,Tatiana Sousa Casarini,Dulce Elena |
| publishDate | 2021 |
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| spelling | Catecholamines production by kidney tissue and mesangial cell culture is differentially modulated by diabetesMoreira,Roseli PeresBertoncello,Nadia S. C.Colucci,Juliana AlmadaArita,Danielle YuriAndrade,Maria Claudina Camargo deRonchi,Fernanda AparecidaCunha,Tatiana SousaCasarini,Dulce ElenaDiabetes MellitusCatecholaminesNorepinephrineEpinephrineDopamineopen accesshttp://purl.org/coar/access_right/c_abf2http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-28002021000400510URLhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-28002021000400510URLHasVersion2021-12-01Abstract Introduction: According to the International Diabetes Federation, the number of people with diabetes mellitus may reach 700 million in 2045. Catecholamines are involved in the regulation of several kidney functions. This study investigates the effects of hyperglycemia on catecholamines' metabolism in kidney tissue from control, diabetic, and insulin-treated diabetic rats, both in vivo and in vitro. Methods: Male Wistar-Hannover rats were randomized into: control, diabetic, and insulin-treated diabetic groups. Diabetes was induced by a single injection of streptozotocin, and diabetic treated group also received insulin. After 60 days, blood and kidney tissue from all groups were collected for catecholamines' quantification and mesangial cells culture. Results: diabetic rats had lower body weight, hyperglycemia, and increase water intake and diuresis. Additionally, diabetes promoted a sharp decrease in creatinine clearance compared to control group. Regarding the whole kidney extracts, both diabetic groups (treated and non-treated) had significant reduction in norepinephrine concentration. In mesangial cell culture, catecholamines' concentration were lower in the culture medium than in the intracellular compartment for all groups. Norepinephrine, epinephrine, and dopamine medium levels were increased in the diabetic group. Conclusion: The major finding of the present study was that 8 weeks of diabetes induction altered the kidney catecholaminergic system in a very specific manner, once the production of catecholamines in the excised kidney tissue from diabetic rats was differentially modulated as compared with the production and secretion by cultured mesangial cells.engtext/htmlSociedade Brasileira de NefrologiaBrazilian Journal of Nephrology v.43 n.4 2021reponame:Jornal Brasileiro de Nefrologiainstname:Sociedade Brasileira de Nefrologia (SBN)instacron:SBNjournal articlehttp://purl.org/coar/resource_type/c_6501literatureVoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85 |
| spellingShingle | Catecholamines production by kidney tissue and mesangial cell culture is differentially modulated by diabetes Moreira,Roseli Peres Diabetes Mellitus Catecholamines Norepinephrine Epinephrine Dopamine |
| status | SINGLETON |
| status_str | VoR |
| subject.fl_str_mv | Diabetes Mellitus Catecholamines Norepinephrine Epinephrine Dopamine |
| title | Catecholamines production by kidney tissue and mesangial cell culture is differentially modulated by diabetes |
| title_full | Catecholamines production by kidney tissue and mesangial cell culture is differentially modulated by diabetes |
| title_fullStr | Catecholamines production by kidney tissue and mesangial cell culture is differentially modulated by diabetes |
| title_full_unstemmed | Catecholamines production by kidney tissue and mesangial cell culture is differentially modulated by diabetes |
| title_short | Catecholamines production by kidney tissue and mesangial cell culture is differentially modulated by diabetes |
| title_sort | Catecholamines production by kidney tissue and mesangial cell culture is differentially modulated by diabetes |
| topic | Diabetes Mellitus Catecholamines Norepinephrine Epinephrine Dopamine |
| topic_facet | Diabetes Mellitus Catecholamines Norepinephrine Epinephrine Dopamine |
| url | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-28002021000400510 |
| visible | 1 |
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