Publicação
Metallothionein expression and its influence on the in vitro biological behavior of mucoepidermoid carcinoma
| Resumo: | This research was funded by the Brazilian National Council for Scientific and Technological Development, grant number 47409720129. |
|---|---|
| Autores principais: | Aquime, João Rafael Habib souza |
| Outros Autores: | Zampieri, Lara Carolina D'Araújo Pinto; Kataoka, MAria Sueli da Silva; Ribeiro, Nelson Antonio Bailão; Jaeger, Ruy Gastaldoni; Silva, Artur Luiz da; Ramos, Rommel Thiago Jucá; Alves Júnior, Sérgio de Melo; Pinheiro, João de Jesus Viana |
| Assunto: | Glândulas Salivares / patologia Carcinoma Mucoepidermoide / diagnóstico Metalotioneína Metaloproteinases da Matriz |
| Ano: | 2020 |
| País: | Brasil |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | IBICT |
| Idioma: | inglês |
| Origem: | Oasisbr |
| _version_ | 1871610454095167488 |
|---|---|
| author | Aquime, João Rafael Habib souza |
| author2 | Zampieri, Lara Carolina D'Araújo Pinto Kataoka, MAria Sueli da Silva Ribeiro, Nelson Antonio Bailão Jaeger, Ruy Gastaldoni Silva, Artur Luiz da Ramos, Rommel Thiago Jucá Alves Júnior, Sérgio de Melo Pinheiro, João de Jesus Viana |
| author2_role | author author author author author author author author |
| author_facet | Aquime, João Rafael Habib souza Zampieri, Lara Carolina D'Araújo Pinto Kataoka, MAria Sueli da Silva Ribeiro, Nelson Antonio Bailão Jaeger, Ruy Gastaldoni Silva, Artur Luiz da Ramos, Rommel Thiago Jucá Alves Júnior, Sérgio de Melo Pinheiro, João de Jesus Viana |
| author_role | author |
| country_str | BR |
| creators_json_txt | [{\"Person.name\":\"Aquime, João Rafael Habib souza\"},{\"Person.name\":\"Zampieri, Lara Carolina D'Araújo Pinto\"},{\"Person.name\":\"Kataoka, MAria Sueli da Silva\"},{\"Person.name\":\"Ribeiro, Nelson Antonio Bailão\"},{\"Person.name\":\"Jaeger, Ruy Gastaldoni\"},{\"Person.name\":\"Silva, Artur Luiz da\"},{\"Person.name\":\"Ramos, Rommel Thiago Jucá\"},{\"Person.name\":\"Alves Júnior, Sérgio de Melo\"},{\"Person.name\":\"Pinheiro, João de Jesus Viana\"}] |
| datacite.creators.creator.creatorName.fl_str_mv | Aquime, João Rafael Habib souza Zampieri, Lara Carolina D'Araújo Pinto Kataoka, MAria Sueli da Silva Ribeiro, Nelson Antonio Bailão Jaeger, Ruy Gastaldoni Silva, Artur Luiz da Ramos, Rommel Thiago Jucá Alves Júnior, Sérgio de Melo Pinheiro, João de Jesus Viana |
| datacite.date.Accepted.fl_str_mv | 2020-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2020-04-07T17:32:22Z |
| datacite.date.embargoed.fl_str_mv | 2020-04-07T17:32:22Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Glândulas Salivares / patologia Carcinoma Mucoepidermoide / diagnóstico Metalotioneína Metaloproteinases da Matriz |
| datacite.titles.title.fl_str_mv | Metallothionein expression and its influence on the in vitro biological behavior of mucoepidermoid carcinoma |
| dc.creator.none.fl_str_mv | Aquime, João Rafael Habib souza Zampieri, Lara Carolina D'Araújo Pinto Kataoka, MAria Sueli da Silva Ribeiro, Nelson Antonio Bailão Jaeger, Ruy Gastaldoni Silva, Artur Luiz da Ramos, Rommel Thiago Jucá Alves Júnior, Sérgio de Melo Pinheiro, João de Jesus Viana |
| dc.date.Accepted.fl_str_mv | 2020-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2020-04-07T17:32:22Z |
| dc.date.embargoed.fl_str_mv | 2020-04-07T17:32:22Z |
| dc.description.none.fl_str_mv | Federal University of Para. School of Dentistry. Department of Oral Pathology. Belém, PA, Brazil. Federal University of Para. School of Dentistry. Department of Oral Pathology. Belém, PA, Brazil. Federal University of Para. School of Dentistry. Department of Oral Pathology. Belém, PA, Brazil. State University of Pará. Laboratory of Genetics and Molecular Biology. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Núcleo de Análise Genética para Imagem. Ananindeua, PA, Brasil. University of São Paulo. Institute of Biomedical Sciences. Department of Cell and Developmental Biology. São Paulo, SP, Brazil. Federal University of Pará. Institute of Biological Sciences. Belém, PA, Brazil. Federal University of Pará. Institute of Biological Sciences. Belém, PA, Brazil. Federal University of Para. School of Dentistry. Department of Oral Pathology. Belém, PA, Brazil. Federal University of Para. School of Dentistry. Department of Oral Pathology. Belém, PA, Brazil / University of São Paulo. Institute of Biomedical Sciences. Department of Cell and Developmental Biology. São Paulo, SP, Brazil. Mucoepidermoid carcinoma (MEC) is the most common tumor in the salivary glands, often presenting with recurrence and metastasis due to its high invasive capacity. Metallothionein (MT), a zinc storage protein that supplies this element for protease activity, is probably related to mucoepidermoid carcinoma behavior. This prompted us to characterize a cell line derived from mucoepidermoid carcinoma and to correlate metallothionein expression with transforming growth factor-α (TGF-α), tumor necrosis factor-α (TNF-α) and matrix metalloproteinases (MMPs). Transcriptomic analysis and cytogenetic assays were performed to detect the expression of genes of interest and cellular chromosomal alterations, respectively. MEC cells with a depleted metallothionein 2A (MT2A) gene were subjected to Western blot to correlate metallothionein expression with growth factors and MMPs. Additionally, cells with depleted MT were subjected to migration and invasion assays. The transcriptomic study revealed reads mapped to cytokeratins 19 and AE1/AE3, α-smooth muscle actin, vimentin, and fibronectin. Cytogenetic evaluation demonstrated structural and numerical alterations, including the translocation t(11;19)(q21;p13), characteristic of MEC. Metallothionein depletion was correlated with the decreased expression of TGF-α and MMP-9, while TNF-α protein levels were augmented. Migration and invasion activity were diminished after metallothionein silencing. Our findings suggest an important role of MT in MEC invasion, through the regulation of proteins involved in this process. |
| dc.identifier.none.fl_str_mv | https://doi.org/10.3390/cells9010157. |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | MDPI |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.source.none.fl_str_mv | reponame:Repositório Digital do Instituto Evandro Chagas (Patuá) instname:Instituto Evandro Chagas (IEC) instacron:IEC |
| dc.subject.none.fl_str_mv | Glândulas Salivares / patologia Carcinoma Mucoepidermoide / diagnóstico Metalotioneína Metaloproteinases da Matriz |
| dc.title.fl_str_mv | Metallothionein expression and its influence on the in vitro biological behavior of mucoepidermoid carcinoma |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | This research was funded by the Brazilian National Council for Scientific and Technological Development, grant number 47409720129. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| id | oasisbr_3fa890ce2700bff7e417a5c263bc052d |
| identifier.doi.fl_str_mv | https://doi.org/10.3390/cells9010157. |
| inst_facet_str | urn:organizationAcronym:oasisbr{{{_:::_}}}IBICT |
| instacron_str | OASISBR |
| institution | IBICT |
| instname_str | IBICT |
| language | eng |
| network_acronym_str | oasisbr |
| network_name_str | Oasisbr |
| oai_identifier_str | oai:patua.iec.gov.br:iec/4083 |
| organization_str_mv | urn:organizationAcronym:oasisbr |
| person_str_mv | Aquime, João Rafael Habib souza Zampieri, Lara Carolina D'Araújo Pinto Kataoka, MAria Sueli da Silva Ribeiro, Nelson Antonio Bailão Jaeger, Ruy Gastaldoni Silva, Artur Luiz da Ramos, Rommel Thiago Jucá Alves Júnior, Sérgio de Melo Pinheiro, João de Jesus Viana |
| publishDate | 2020 |
| publisher.none.fl_str_mv | MDPI |
| repo_facet_str | urn:repositoryAcronym:oasisbr{{{_:::_}}}Oasisbr |
| reponame_str | Oasisbr |
| repository_id_str | urn:repositoryAcronym:oasisbr |
| service_str_mv | urn:repositoryAcronym:oasisbr |
| spelling | Metallothionein expression and its influence on the in vitro biological behavior of mucoepidermoid carcinomaAquime, João Rafael Habib souzaZampieri, Lara Carolina D'Araújo PintoKataoka, MAria Sueli da SilvaRibeiro, Nelson Antonio BailãoJaeger, Ruy GastaldoniSilva, Artur Luiz daRamos, Rommel Thiago JucáAlves Júnior, Sérgio de MeloPinheiro, João de Jesus VianaGlândulas Salivares / patologiaCarcinoma Mucoepidermoide / diagnósticoMetalotioneínaMetaloproteinases da Matrizopen accesshttp://purl.org/coar/access_right/c_abf2https://doi.org/10.3390/cells9010157.DOIAQUIME, João Rafael Habib Souza et al. Metallothionein expression and its influence on the in vitro biological behavior of mucoepidermoid carcinoma. Cells, v. 9, n. 1, E157, 2020.N/AHasVersionhttps://patua.iec.gov.br/handle/iec/4083URLHasVersion2073-4409N/AHasVersionhttps://doi.org/10.3390/cells9010157.DOI2020-04-07T17:32:22Z2020-04-072020-01-01T00:00:00ZThis research was funded by the Brazilian National Council for Scientific and Technological Development, grant number 47409720129.Federal University of Para. School of Dentistry. Department of Oral Pathology. Belém, PA, Brazil.Federal University of Para. School of Dentistry. Department of Oral Pathology. Belém, PA, Brazil.Federal University of Para. School of Dentistry. Department of Oral Pathology. Belém, PA, Brazil.State University of Pará. Laboratory of Genetics and Molecular Biology. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Núcleo de Análise Genética para Imagem. Ananindeua, PA, Brasil.University of São Paulo. Institute of Biomedical Sciences. Department of Cell and Developmental Biology. São Paulo, SP, Brazil.Federal University of Pará. Institute of Biological Sciences. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Belém, PA, Brazil.Federal University of Para. School of Dentistry. Department of Oral Pathology. Belém, PA, Brazil.Federal University of Para. School of Dentistry. Department of Oral Pathology. Belém, PA, Brazil / University of São Paulo. Institute of Biomedical Sciences. Department of Cell and Developmental Biology. São Paulo, SP, Brazil.Mucoepidermoid carcinoma (MEC) is the most common tumor in the salivary glands, often presenting with recurrence and metastasis due to its high invasive capacity. Metallothionein (MT), a zinc storage protein that supplies this element for protease activity, is probably related to mucoepidermoid carcinoma behavior. This prompted us to characterize a cell line derived from mucoepidermoid carcinoma and to correlate metallothionein expression with transforming growth factor-α (TGF-α), tumor necrosis factor-α (TNF-α) and matrix metalloproteinases (MMPs). Transcriptomic analysis and cytogenetic assays were performed to detect the expression of genes of interest and cellular chromosomal alterations, respectively. MEC cells with a depleted metallothionein 2A (MT2A) gene were subjected to Western blot to correlate metallothionein expression with growth factors and MMPs. Additionally, cells with depleted MT were subjected to migration and invasion assays. The transcriptomic study revealed reads mapped to cytokeratins 19 and AE1/AE3, α-smooth muscle actin, vimentin, and fibronectin. Cytogenetic evaluation demonstrated structural and numerical alterations, including the translocation t(11;19)(q21;p13), characteristic of MEC. Metallothionein depletion was correlated with the decreased expression of TGF-α and MMP-9, while TNF-α protein levels were augmented. Migration and invasion activity were diminished after metallothionein silencing. Our findings suggest an important role of MT in MEC invasion, through the regulation of proteins involved in this process.engMDPIreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECVoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85journal articlehttp://purl.org/coar/resource_type/c_6501literature |
| spellingShingle | Metallothionein expression and its influence on the in vitro biological behavior of mucoepidermoid carcinoma Aquime, João Rafael Habib souza Glândulas Salivares / patologia Carcinoma Mucoepidermoide / diagnóstico Metalotioneína Metaloproteinases da Matriz |
| status | SINGLETON |
| status_str | VoR |
| subject.fl_str_mv | Glândulas Salivares / patologia Carcinoma Mucoepidermoide / diagnóstico Metalotioneína Metaloproteinases da Matriz |
| title | Metallothionein expression and its influence on the in vitro biological behavior of mucoepidermoid carcinoma |
| title_full | Metallothionein expression and its influence on the in vitro biological behavior of mucoepidermoid carcinoma |
| title_fullStr | Metallothionein expression and its influence on the in vitro biological behavior of mucoepidermoid carcinoma |
| title_full_unstemmed | Metallothionein expression and its influence on the in vitro biological behavior of mucoepidermoid carcinoma |
| title_short | Metallothionein expression and its influence on the in vitro biological behavior of mucoepidermoid carcinoma |
| title_sort | Metallothionein expression and its influence on the in vitro biological behavior of mucoepidermoid carcinoma |
| topic | Glândulas Salivares / patologia Carcinoma Mucoepidermoide / diagnóstico Metalotioneína Metaloproteinases da Matriz |
| topic_facet | Glândulas Salivares / patologia Carcinoma Mucoepidermoide / diagnóstico Metalotioneína Metaloproteinases da Matriz |
| url | https://doi.org/10.3390/cells9010157. |
| visible | 1 |
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