Publicação
Evolution of PTH assays
| Resumo: | PTH metabolism is complex and the circulating forms include the intact 1-84 molecule as well as several carboxyl-terminal fragments. The first generation of PTH assays included several types of competitive assays, with specificities that spanned carboxyl, mid-region and amino-terminal portions of the molecule. The limitations of these assays and the methodological evolution led to the description of 2nd generation non-competitive immunometric assays for PTH in the late 80's, based on the recognition of the PTH molecule by two different antibodies, one directed against de amino-terminal and other against the carboxyl-terminal segments. The observation that in some circumstances "long" carboxyl-terminal segments were also measured by 2nd generation assays led to the development of 3rd generation assays based on amino-terminal specific antibodies that are specific for the first amino acids, measuring only the molecular forms that activate PTH1R. The practical and cost-benefit advantages of these assays are still debatable. The recent observation that carboxyl-terminal fragments of PTH have biological activity via a distinct receptor than PTH1R, points to the future need of more than one assay in order to evaluate parathyroid hormone function. |
|---|---|
| Autores principais: | Vieira,José Gilberto H. |
| Outros Autores: | Kunii,Ilda; Nishida,Sônia |
| Assunto: | Parathyroid hormone assay Radioimmunoassay Immunometric assays Circulating forms of parathyroid hormone |
| Ano: | 2006 |
| País: | Brasil |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | IBICT |
| Idioma: | inglês |
| Origem: | Oasisbr |
| _version_ | 1871511903028641792 |
|---|---|
| author | Vieira,José Gilberto H. |
| author2 | Kunii,Ilda Nishida,Sônia |
| author2_role | author author |
| author_facet | Vieira,José Gilberto H. Kunii,Ilda Nishida,Sônia |
| author_role | author |
| country_str | BR |
| creators_json_txt | [{\"Person.name\":\"Vieira,José Gilberto H.\"},{\"Person.name\":\"Kunii,Ilda\"},{\"Person.name\":\"Nishida,Sônia\"}] |
| datacite.creators.creator.creatorName.fl_str_mv | Vieira,José Gilberto H. Kunii,Ilda Nishida,Sônia |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Parathyroid hormone assay Radioimmunoassay Immunometric assays Circulating forms of parathyroid hormone |
| datacite.titles.title.fl_str_mv | Evolution of PTH assays |
| dc.creator.none.fl_str_mv | Vieira,José Gilberto H. Kunii,Ilda Nishida,Sônia |
| dc.format.none.fl_str_mv | text/html |
| dc.identifier.none.fl_str_mv | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27302006000400007 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Sociedade Brasileira de Endocrinologia e Metabologia |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.source.none.fl_str_mv | Arquivos Brasileiros de Endocrinologia & Metabologia v.50 n.4 2006 reponame:Arquivos Brasileiros de Endocrinologia & Metabologia (Online) instname:Sociedade Brasileira de Endocrinologia e Metabologia (SBEM) instacron:SBEM |
| dc.subject.none.fl_str_mv | Parathyroid hormone assay Radioimmunoassay Immunometric assays Circulating forms of parathyroid hormone |
| dc.title.fl_str_mv | Evolution of PTH assays |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | PTH metabolism is complex and the circulating forms include the intact 1-84 molecule as well as several carboxyl-terminal fragments. The first generation of PTH assays included several types of competitive assays, with specificities that spanned carboxyl, mid-region and amino-terminal portions of the molecule. The limitations of these assays and the methodological evolution led to the description of 2nd generation non-competitive immunometric assays for PTH in the late 80's, based on the recognition of the PTH molecule by two different antibodies, one directed against de amino-terminal and other against the carboxyl-terminal segments. The observation that in some circumstances "long" carboxyl-terminal segments were also measured by 2nd generation assays led to the development of 3rd generation assays based on amino-terminal specific antibodies that are specific for the first amino acids, measuring only the molecular forms that activate PTH1R. The practical and cost-benefit advantages of these assays are still debatable. The recent observation that carboxyl-terminal fragments of PTH have biological activity via a distinct receptor than PTH1R, points to the future need of more than one assay in order to evaluate parathyroid hormone function. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| id | oasisbr_2b88797c420766765ba77d35cbd7fe97 |
| identifier.url.fl_str_mv | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27302006000400007 |
| inst_facet_str | urn:organizationAcronym:oasisbr{{{_:::_}}}IBICT |
| instacron_str | OASISBR |
| institution | IBICT |
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| language | eng |
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| oai_identifier_str | oai:scielo:S0004-27302006000400007 |
| organization_str_mv | urn:organizationAcronym:oasisbr |
| person_str_mv | Vieira,José Gilberto H. Kunii,Ilda Nishida,Sônia |
| publishDate | 2006 |
| publisher.none.fl_str_mv | Sociedade Brasileira de Endocrinologia e Metabologia |
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| spelling | Evolution of PTH assaysVieira,José Gilberto H.Kunii,IldaNishida,SôniaParathyroid hormone assayRadioimmunoassayImmunometric assaysCirculating forms of parathyroid hormoneopen accesshttp://purl.org/coar/access_right/c_abf2http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27302006000400007URLhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27302006000400007URLHasVersion2006-08-01PTH metabolism is complex and the circulating forms include the intact 1-84 molecule as well as several carboxyl-terminal fragments. The first generation of PTH assays included several types of competitive assays, with specificities that spanned carboxyl, mid-region and amino-terminal portions of the molecule. The limitations of these assays and the methodological evolution led to the description of 2nd generation non-competitive immunometric assays for PTH in the late 80's, based on the recognition of the PTH molecule by two different antibodies, one directed against de amino-terminal and other against the carboxyl-terminal segments. The observation that in some circumstances "long" carboxyl-terminal segments were also measured by 2nd generation assays led to the development of 3rd generation assays based on amino-terminal specific antibodies that are specific for the first amino acids, measuring only the molecular forms that activate PTH1R. The practical and cost-benefit advantages of these assays are still debatable. The recent observation that carboxyl-terminal fragments of PTH have biological activity via a distinct receptor than PTH1R, points to the future need of more than one assay in order to evaluate parathyroid hormone function.engtext/htmlSociedade Brasileira de Endocrinologia e MetabologiaArquivos Brasileiros de Endocrinologia & Metabologia v.50 n.4 2006reponame:Arquivos Brasileiros de Endocrinologia & Metabologia (Online)instname:Sociedade Brasileira de Endocrinologia e Metabologia (SBEM)instacron:SBEMjournal articlehttp://purl.org/coar/resource_type/c_6501literatureVoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85 |
| spellingShingle | Evolution of PTH assays Vieira,José Gilberto H. Parathyroid hormone assay Radioimmunoassay Immunometric assays Circulating forms of parathyroid hormone |
| status | SINGLETON |
| status_str | VoR |
| subject.fl_str_mv | Parathyroid hormone assay Radioimmunoassay Immunometric assays Circulating forms of parathyroid hormone |
| title | Evolution of PTH assays |
| title_full | Evolution of PTH assays |
| title_fullStr | Evolution of PTH assays |
| title_full_unstemmed | Evolution of PTH assays |
| title_short | Evolution of PTH assays |
| title_sort | Evolution of PTH assays |
| topic | Parathyroid hormone assay Radioimmunoassay Immunometric assays Circulating forms of parathyroid hormone |
| topic_facet | Parathyroid hormone assay Radioimmunoassay Immunometric assays Circulating forms of parathyroid hormone |
| url | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27302006000400007 |
| visible | 1 |
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