Publicação
Low cost microfluidic device for partial cell separation: micromilling approach
| Resumo: | Several studies have already demonstrated that it is possible to perform blood flow studies in microfluidic systems fabricated by using low-cost techniques. However, most of these techniques do not produce microchannels smaller than 100 microns and as a result they have several limitations related to blood cell separation. Recently, manufacturers have been able to produce milling tools smaller than 100 microns, which consequently have promoted the ability of micromilling machines to fabricate microfluidic devices able to perform separation of red blood cells (RBCs) from plasma. In this work, we show the ability of a micromilling machine to manufacture microchannels with dimensions down to 30 microns. Additionally, we show for the first time the ability of the proposed microfluidic device to enhance the cell-free layer close to the walls, leading to perform partial separation of RBCs from plasma. |
|---|---|
| Autores principais: | Lopes, Raquel |
| Outros Autores: | Rodrigues, Raquel Oliveira; Pinho, Diana; Valdemar, Garcia; Schütte, Helmut; Lima, Rui A.; Gassmann, Stefan |
| Assunto: | Micromilling Microfluidic device Nonlithographic technique Red blood cells Cell-free layer |
| Ano: | 2015 |
| País: | Portugal |
| Tipo de documento: | comunicação em conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Politécnica de Bragança |
| Idioma: | português |
| Origem: | Biblioteca Digital da UPB |
| _version_ | 1877782274527723520 |
|---|---|
| author | Lopes, Raquel |
| author2 | Rodrigues, Raquel Oliveira Pinho, Diana Valdemar, Garcia Schütte, Helmut Lima, Rui A. Gassmann, Stefan |
| author2_role | author author author author author author |
| author_facet | Lopes, Raquel Rodrigues, Raquel Oliveira Pinho, Diana Valdemar, Garcia Schütte, Helmut Lima, Rui A. Gassmann, Stefan |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital da UPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Lopes, Raquel\"},{\"Person.name\":\"Rodrigues, Raquel Oliveira\"},{\"Person.name\":\"Pinho, Diana\",\"Person.identifier.cienciaID\":\"5214-ED0C-35E8\",\"Person.identifier.orcid\":\"0000-0002-3884-6496\"},{\"Person.name\":\"Valdemar, Garcia\"},{\"Person.name\":\"Schütte, Helmut\"},{\"Person.name\":\"Lima, Rui A.\",\"Person.identifier.cienciaID\":\"EE12-C3FB-349D\",\"Person.identifier.orcid\":\"0000-0003-3428-637X\",\"Person.identifier.researcherID\":\"H-5157-2016\",\"Person.identifier.scopusAuthorID\":\"18437397800\"},{\"Person.name\":\"Gassmann, Stefan\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital da UPB |
| datacite.creators.creator.creatorName.fl_str_mv | Lopes, Raquel Rodrigues, Raquel Oliveira Pinho, Diana Valdemar, Garcia Schütte, Helmut Lima, Rui A. Gassmann, Stefan |
| datacite.date.Accepted.fl_str_mv | 2015-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2015-07-31T10:18:22Z |
| datacite.date.embargoed.fl_str_mv | 2015-07-31T10:18:22Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Micromilling Microfluidic device Nonlithographic technique Red blood cells Cell-free layer |
| datacite.titles.title.fl_str_mv | Low cost microfluidic device for partial cell separation: micromilling approach |
| dc.contributor.none.fl_str_mv | Biblioteca Digital da UPB |
| dc.creator.none.fl_str_mv | Lopes, Raquel Rodrigues, Raquel Oliveira Pinho, Diana Valdemar, Garcia Schütte, Helmut Lima, Rui A. Gassmann, Stefan |
| dc.date.Accepted.fl_str_mv | 2015-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2015-07-31T10:18:22Z |
| dc.date.embargoed.fl_str_mv | 2015-07-31T10:18:22Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/12014 |
| dc.language.none.fl_str_mv | por |
| dc.publisher.none.fl_str_mv | IEEE |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Micromilling Microfluidic device Nonlithographic technique Red blood cells Cell-free layer |
| dc.title.fl_str_mv | Low cost microfluidic device for partial cell separation: micromilling approach |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_5794 |
| description | Several studies have already demonstrated that it is possible to perform blood flow studies in microfluidic systems fabricated by using low-cost techniques. However, most of these techniques do not produce microchannels smaller than 100 microns and as a result they have several limitations related to blood cell separation. Recently, manufacturers have been able to produce milling tools smaller than 100 microns, which consequently have promoted the ability of micromilling machines to fabricate microfluidic devices able to perform separation of red blood cells (RBCs) from plasma. In this work, we show the ability of a micromilling machine to manufacture microchannels with dimensions down to 30 microns. Additionally, we show for the first time the ability of the proposed microfluidic device to enhance the cell-free layer close to the walls, leading to perform partial separation of RBCs from plasma. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | conferencePaper |
| fulltext.url.fl_str_mv | https://bibliotecadigital.unipb.pt/bitstreams/4093772d-edc3-453b-baf3-236349e1f7bf/download |
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| funding.funder.alternateName_str_mv | FCT FCT FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.identifier_str_mv | PTDC/SAU-ENB/116929/2010 EXPL/EMS-SIS/2215/2013 SFRH/BD/97658/2013 SFRH/BD/89077/2012 |
| funding.name_str_mv | 5876-PPCDTI COMPETE SFRH |
| funding_str_mv | PTDC/SAU-ENB/116929/2010 info:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FSAU-ENB%2F116929%2F2010/PT EXPL/EMS-SIS/2215/2013 info:eu-repo/grantAgreement/FCT/COMPETE/EXPL%2FEMS-SIS%2F2215%2F2013/PT SFRH/BD/97658/2013 info:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F97658%2F2013/PT SFRH/BD/89077/2012 info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F89077%2F2012/PT |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10198/12014 |
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| language | por |
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| network_name_str | Biblioteca Digital da UPB |
| oai_identifier_str | oai:bibliotecadigital.unipb.pt:10198/12014 |
| organization_str_mv | urn:organizationAcronym:unipb |
| person_str_mv | Lopes, Raquel Rodrigues, Raquel Oliveira Pinho, Diana Pinho, Diana https://www.ciencia-id.pt/5214-ED0C-35E8 5214-ED0C-35E8 http://orcid.org/0000-0002-3884-6496 0000-0002-3884-6496 Valdemar, Garcia Schütte, Helmut Lima, Rui A. Lima, Rui A. https://www.ciencia-id.pt/EE12-C3FB-349D EE12-C3FB-349D http://orcid.org/0000-0003-3428-637X 0000-0003-3428-637X Gassmann, Stefan |
| publishDate | 2015 |
| publisher.none.fl_str_mv | IEEE |
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| reponame_str | Biblioteca Digital da UPB |
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| spelling | porIEEEporSeveral studies have already demonstrated that it is possible to perform blood flow studies in microfluidic systems fabricated by using low-cost techniques. However, most of these techniques do not produce microchannels smaller than 100 microns and as a result they have several limitations related to blood cell separation. Recently, manufacturers have been able to produce milling tools smaller than 100 microns, which consequently have promoted the ability of micromilling machines to fabricate microfluidic devices able to perform separation of red blood cells (RBCs) from plasma. In this work, we show the ability of a micromilling machine to manufacture microchannels with dimensions down to 30 microns. Additionally, we show for the first time the ability of the proposed microfluidic device to enhance the cell-free layer close to the walls, leading to perform partial separation of RBCs from plasma.application/pdfporLow cost microfluidic device for partial cell separation: micromilling approachLopes, RaquelRodrigues, Raquel OliveiraPersonalPinho, DianaDSpacehttp://dspace.org/items/02bf2dd9-33ed-4d87-b61d-ca39c51f8451DSpacehttp://dspace.org/items/02bf2dd9-33ed-4d87-b61d-ca39c51f8451PinhoDianaCiência IDhttps://www.ciencia-id.pt5214-ED0C-35E8ORCIDhttp://orcid.org0000-0002-3884-6496Valdemar, GarciaSchütte, HelmutPersonalLima, Rui A.DSpacehttp://dspace.org/items/7b50c499-8095-4f4f-8b1b-fa7388e4ff62DSpacehttp://dspace.org/items/7b50c499-8095-4f4f-8b1b-fa7388e4ff62LimaRui A.Ciência IDhttps://www.ciencia-id.ptEE12-C3FB-349DORCIDhttp://orcid.org0000-0003-3428-637XResearcher IDhttps://www.researcherid.comH-5157-2016Scopus Author IDhttps://www.scopus.com18437397800Gassmann, StefanHostingInstitutionOrganizationalBiblioteca Digital da UPBe-mailmailto:dspace@unipb.ptdspace@unipb.ptISBNIsPartOf978-1-4799-7800-7DOIIsPartOf10.1109/ICIT.2015.71255942015-07-31T10:18:22Z20152015-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/12014http://purl.org/coar/access_right/c_abf2open accessMicromillingMicrofluidic deviceNonlithographic techniqueRed blood cellsCell-free layer1343346 bytesFundação para a Ciência e a TecnologiaBIOMIMETIC - A biomimetic microdevice for the diagnosis of erythrocytes diseases based on their deformabilityinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FSAU-ENB%2F116929%2F2010/PTPTDC/SAU-ENB/116929/20105876-PPCDTICrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaMicroBloodSystems - O estudo da reologia do sangue e a migração das células sanguíneas em sistemas microfluídicosinfo:eu-repo/grantAgreement/FCT/COMPETE/EXPL%2FEMS-SIS%2F2215%2F2013/PTEXPL/EMS-SIS/2215/2013COMPETECrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaMagnetic carbon nanostructures and study of their transport in microfluidic devices for hyperthermiainfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F97658%2F2013/PTSFRH/BD/97658/2013Crossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaBlood Rheology and Red Blood Cell Migration in Microchannel Flowsinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F89077%2F2012/PTSFRH/BD/89077/2012SFRHCrossref Funder IDhttp://doi.org/10.13039/501100001871other research producthttp://purl.org/coar/resource_type/c_5794conference paperhttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.unipb.pt/bitstreams/4093772d-edc3-453b-baf3-236349e1f7bf/downloadIndustrial Technology33473350Seville, Spain |
| spellingShingle | Low cost microfluidic device for partial cell separation: micromilling approach Lopes, Raquel Micromilling Microfluidic device Nonlithographic technique Red blood cells Cell-free layer |
| status | SINGLETON |
| subject.fl_str_mv | Micromilling Microfluidic device Nonlithographic technique Red blood cells Cell-free layer |
| title | Low cost microfluidic device for partial cell separation: micromilling approach |
| title_full | Low cost microfluidic device for partial cell separation: micromilling approach |
| title_fullStr | Low cost microfluidic device for partial cell separation: micromilling approach |
| title_full_unstemmed | Low cost microfluidic device for partial cell separation: micromilling approach |
| title_short | Low cost microfluidic device for partial cell separation: micromilling approach |
| title_sort | Low cost microfluidic device for partial cell separation: micromilling approach |
| topic | Micromilling Microfluidic device Nonlithographic technique Red blood cells Cell-free layer |
| topic_facet | Micromilling Microfluidic device Nonlithographic technique Red blood cells Cell-free layer |
| url | http://hdl.handle.net/10198/12014 |
| visible | 1 |