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Study of the antioxidant, antimicrobial and anti-inflammatory activities of two Euphorbia species
| Resumo: | In this research, a study of the antioxidant, antimicrobial and anti-inflammatory activities of two Euphorbia species (Euphorbia hirta and Euphorbia jolkinii) was developed. Euphorbia species belong to the Euphorbiaceae family, being commonly found in America and tropical Africa. Euphorbia species have been traditionally used in folk medicine to treat gonorrhea, migraines, intestinal parasites, warts, and skin diseases [1]. Since there is more data available of Euphorbia hirta, the aim of this study was to provide a comparison between E. hirta and E. jokinii regarding their antioxidant, antimicrobial and anti-inflammatory activities. For the cellular antioxidant activity, murine macrophage cells RAW 264.7 were used following the procedure described by Wolf & Lui (2007). E. hirta showed better inhibition percentage results than E. jolkinii, being 2,000 μg/mL, the maximum concentration tested for both samples. Extracts of both Euphorbia species were used to determine their antimicrobial activity. Gram-negative and Gram-positive food and clinical bacteria were tested for this assay. Moreover, Streptomycin, Methicillin and Ampicillin were used as controllers to compare both MIC and MBC results. For the food bacteria, E. jolkinii had better MIC results than E. hirta when Gram-negative bacteria were tested, although it was lower than controllers. MBC results for both Euphorbia species were similar and lower than the controllers. When Gram-positive clinical bacteria were tested, similar MIC results between both extracts were obtained. However, when L. monocytogenes and S. aureus were tested, E. jolkinii showed better MIC results. Antifungal activity was also assessed using ketoconazole as control and Aspergillus brasiliensis and Aspergillus fumigatus as fungi. Unsatisfactory results were obtained for both Euphorbia species for both fungi. AGS, CaCo2, MCF-7, NCl-H460, PLP2 and RAW 264.7 cell lines were used for the anti-inflammatory assay. Ellipticine was used as control in all the cell lines except in RAW 264.7, where dexamethasone was used. Results showed lower anti-inflammatory activity of the 2 extracts compared to the controllers in all cell lines, being E. jolkinii the one with lower GI50. Considering the obtained results, although E. hirta has been widely studied, E. jolkinii showed better antimicrobial and anti-inflammatory activities, being a suitable option for the pharmacological industry. |
|---|---|
| Autores principais: | Perez Vazquez, Ana |
| Outros Autores: | Alvarez, Paula Barciela; Silva, Tayse Ferreira; Rodrigues, Daniele Bobrowski; Mandim, Filipa; Pires, Tânia C.S.; Carpena Rodríguez, María; Simal-Gandara, Jesus; Barros, Lillian; Prieto Lage, Miguel A. |
| Assunto: | Euphorbia Research Subject Categories::MEDICINE::Morphology, cell biology, pathology |
| Ano: | 2022 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Politécnica de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital da UPB |
| _version_ | 1877782858992451584 |
|---|---|
| author | Perez Vazquez, Ana |
| author2 | Alvarez, Paula Barciela Silva, Tayse Ferreira Rodrigues, Daniele Bobrowski Mandim, Filipa Pires, Tânia C.S. Carpena Rodríguez, María Simal-Gandara, Jesus Barros, Lillian Prieto Lage, Miguel A. |
| author2_role | author author author author author author author author author |
| author_facet | Perez Vazquez, Ana Alvarez, Paula Barciela Silva, Tayse Ferreira Rodrigues, Daniele Bobrowski Mandim, Filipa Pires, Tânia C.S. Carpena Rodríguez, María Simal-Gandara, Jesus Barros, Lillian Prieto Lage, Miguel A. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital da UPB |
| country_str | PT |
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| datacite.creators.creator.creatorName.fl_str_mv | Perez Vazquez, Ana Alvarez, Paula Barciela Silva, Tayse Ferreira Rodrigues, Daniele Bobrowski Mandim, Filipa Pires, Tânia C.S. Carpena Rodríguez, María Simal-Gandara, Jesus Barros, Lillian Prieto Lage, Miguel A. |
| datacite.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2023-03-17T14:14:17Z |
| datacite.date.embargoed.fl_str_mv | 2023-03-17T14:14:17Z |
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| datacite.subjects.subject.fl_str_mv | Euphorbia Research Subject Categories::MEDICINE::Morphology, cell biology, pathology |
| datacite.titles.title.fl_str_mv | Study of the antioxidant, antimicrobial and anti-inflammatory activities of two Euphorbia species |
| dc.contributor.none.fl_str_mv | Biblioteca Digital da UPB |
| dc.creator.none.fl_str_mv | Perez Vazquez, Ana Alvarez, Paula Barciela Silva, Tayse Ferreira Rodrigues, Daniele Bobrowski Mandim, Filipa Pires, Tânia C.S. Carpena Rodríguez, María Simal-Gandara, Jesus Barros, Lillian Prieto Lage, Miguel A. |
| dc.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2023-03-17T14:14:17Z |
| dc.date.embargoed.fl_str_mv | 2023-03-17T14:14:17Z |
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| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Universidade de Santiago de Compostela |
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| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Euphorbia Research Subject Categories::MEDICINE::Morphology, cell biology, pathology |
| dc.title.fl_str_mv | Study of the antioxidant, antimicrobial and anti-inflammatory activities of two Euphorbia species |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | In this research, a study of the antioxidant, antimicrobial and anti-inflammatory activities of two Euphorbia species (Euphorbia hirta and Euphorbia jolkinii) was developed. Euphorbia species belong to the Euphorbiaceae family, being commonly found in America and tropical Africa. Euphorbia species have been traditionally used in folk medicine to treat gonorrhea, migraines, intestinal parasites, warts, and skin diseases [1]. Since there is more data available of Euphorbia hirta, the aim of this study was to provide a comparison between E. hirta and E. jokinii regarding their antioxidant, antimicrobial and anti-inflammatory activities. For the cellular antioxidant activity, murine macrophage cells RAW 264.7 were used following the procedure described by Wolf & Lui (2007). E. hirta showed better inhibition percentage results than E. jolkinii, being 2,000 μg/mL, the maximum concentration tested for both samples. Extracts of both Euphorbia species were used to determine their antimicrobial activity. Gram-negative and Gram-positive food and clinical bacteria were tested for this assay. Moreover, Streptomycin, Methicillin and Ampicillin were used as controllers to compare both MIC and MBC results. For the food bacteria, E. jolkinii had better MIC results than E. hirta when Gram-negative bacteria were tested, although it was lower than controllers. MBC results for both Euphorbia species were similar and lower than the controllers. When Gram-positive clinical bacteria were tested, similar MIC results between both extracts were obtained. However, when L. monocytogenes and S. aureus were tested, E. jolkinii showed better MIC results. Antifungal activity was also assessed using ketoconazole as control and Aspergillus brasiliensis and Aspergillus fumigatus as fungi. Unsatisfactory results were obtained for both Euphorbia species for both fungi. AGS, CaCo2, MCF-7, NCl-H460, PLP2 and RAW 264.7 cell lines were used for the anti-inflammatory assay. Ellipticine was used as control in all the cell lines except in RAW 264.7, where dexamethasone was used. Results showed lower anti-inflammatory activity of the 2 extracts compared to the controllers in all cell lines, being E. jolkinii the one with lower GI50. Considering the obtained results, although E. hirta has been widely studied, E. jolkinii showed better antimicrobial and anti-inflammatory activities, being a suitable option for the pharmacological industry. |
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| person_str_mv | Perez Vazquez, Ana Alvarez, Paula Barciela Silva, Tayse Ferreira Rodrigues, Daniele Bobrowski Rodrigues, Daniele Bobrowski https://www.ciencia-id.pt/A91B-FE1D-B275 A91B-FE1D-B275 http://orcid.org/0000-0003-0260-5751 0000-0003-0260-5751 Mandim, Filipa Mandim, Filipa https://www.ciencia-id.pt/F318-9049-EB99 F318-9049-EB99 http://orcid.org/0000-0002-6494-2326 0000-0002-6494-2326 Pires, Tânia C.S. Pires, Tânia C.S. https://www.ciencia-id.pt/321E-2D96-00CA 321E-2D96-00CA http://orcid.org/0000-0002-3954-3833 0000-0002-3954-3833 Carpena Rodríguez, María Simal-Gandara, Jesus Barros, Lillian Barros, Lillian https://www.ciencia-id.pt/9616-35CB-D001 9616-35CB-D001 http://orcid.org/0000-0002-9050-5189 0000-0002-9050-5189 Prieto Lage, Miguel A. Prieto Lage, Miguel A. https://www.ciencia-id.pt/221E-1E00-AB74 221E-1E00-AB74 http://orcid.org/0000-0002-3513-0054 0000-0002-3513-0054 |
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| spelling | engUniversidade de Santiago de Compostelapt_PTIn this research, a study of the antioxidant, antimicrobial and anti-inflammatory activities of two Euphorbia species (Euphorbia hirta and Euphorbia jolkinii) was developed. Euphorbia species belong to the Euphorbiaceae family, being commonly found in America and tropical Africa. Euphorbia species have been traditionally used in folk medicine to treat gonorrhea, migraines, intestinal parasites, warts, and skin diseases [1]. Since there is more data available of Euphorbia hirta, the aim of this study was to provide a comparison between E. hirta and E. jokinii regarding their antioxidant, antimicrobial and anti-inflammatory activities. For the cellular antioxidant activity, murine macrophage cells RAW 264.7 were used following the procedure described by Wolf & Lui (2007). E. hirta showed better inhibition percentage results than E. jolkinii, being 2,000 μg/mL, the maximum concentration tested for both samples. Extracts of both Euphorbia species were used to determine their antimicrobial activity. Gram-negative and Gram-positive food and clinical bacteria were tested for this assay. Moreover, Streptomycin, Methicillin and Ampicillin were used as controllers to compare both MIC and MBC results. For the food bacteria, E. jolkinii had better MIC results than E. hirta when Gram-negative bacteria were tested, although it was lower than controllers. MBC results for both Euphorbia species were similar and lower than the controllers. When Gram-positive clinical bacteria were tested, similar MIC results between both extracts were obtained. However, when L. monocytogenes and S. aureus were tested, E. jolkinii showed better MIC results. Antifungal activity was also assessed using ketoconazole as control and Aspergillus brasiliensis and Aspergillus fumigatus as fungi. Unsatisfactory results were obtained for both Euphorbia species for both fungi. AGS, CaCo2, MCF-7, NCl-H460, PLP2 and RAW 264.7 cell lines were used for the anti-inflammatory assay. Ellipticine was used as control in all the cell lines except in RAW 264.7, where dexamethasone was used. Results showed lower anti-inflammatory activity of the 2 extracts compared to the controllers in all cell lines, being E. jolkinii the one with lower GI50. Considering the obtained results, although E. hirta has been widely studied, E. jolkinii showed better antimicrobial and anti-inflammatory activities, being a suitable option for the pharmacological industry.application/pdfpt_PTStudy of the antioxidant, antimicrobial and anti-inflammatory activities of two Euphorbia speciesPerez Vazquez, AnaAlvarez, Paula BarcielaSilva, Tayse FerreiraPersonalRodrigues, Daniele BobrowskiDSpacehttp://dspace.org/items/18a661e9-0f77-4bd1-b2b0-5b929b760ab3DSpacehttp://dspace.org/items/18a661e9-0f77-4bd1-b2b0-5b929b760ab3RodriguesDaniele BobrowskiCiência IDhttps://www.ciencia-id.ptA91B-FE1D-B275ORCIDhttp://orcid.org0000-0003-0260-5751PersonalMandim, FilipaDSpacehttp://dspace.org/items/bcc274d4-7e6c-46a8-8e49-2752f9dbfbcdDSpacehttp://dspace.org/items/bcc274d4-7e6c-46a8-8e49-2752f9dbfbcdMandimFilipaCiência IDhttps://www.ciencia-id.ptF318-9049-EB99ORCIDhttp://orcid.org0000-0002-6494-2326PersonalPires, Tânia C.S.DSpacehttp://dspace.org/items/34af4d18-b8ae-4329-9450-69dc35ed448fDSpacehttp://dspace.org/items/34af4d18-b8ae-4329-9450-69dc35ed448fPiresTânia C.S.P.Ciência IDhttps://www.ciencia-id.pt321E-2D96-00CAORCIDhttp://orcid.org0000-0002-3954-3833Researcher IDhttps://www.researcherid.comQ-2842-2018Scopus Author IDhttps://www.scopus.com57057440000Carpena Rodríguez, MaríaSimal-Gandara, JesusPersonalBarros, LillianDSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01DSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01BarrosLillianCiência IDhttps://www.ciencia-id.pt9616-35CB-D001ORCIDhttp://orcid.org0000-0002-9050-5189Researcher IDhttps://www.researcherid.comJ-3600-2013Researcher IDhttps://www.researcherid.comJ-3600-2013Scopus Author IDhttps://www.scopus.com35236343600Scopus Author IDhttps://www.scopus.com57194900801PersonalPrieto Lage, Miguel A.DSpacehttp://dspace.org/items/13260615-fd28-4b8b-9dd4-8a8466007579DSpacehttp://dspace.org/items/13260615-fd28-4b8b-9dd4-8a8466007579PrietoMiguel A.Ciência IDhttps://www.ciencia-id.pt221E-1E00-AB74ORCIDhttp://orcid.org0000-0002-3513-0054Researcher IDhttps://www.researcherid.comG-4516-2011Scopus Author IDhttps://www.scopus.com35937495700Scopus Author IDhttps://www.scopus.com57207755963HostingInstitutionOrganizationalBiblioteca Digital da UPBe-mailmailto:dspace@unipb.ptdspace@unipb.ptISBNIsPartOf978-84-09-45895-02023-03-17T14:14:17Z20222022-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/27812http://purl.org/coar/access_right/c_abf2open accessEuphorbiaResearch Subject Categories::MEDICINE::Morphology, cell biology, pathology417994 bytesFundação para a Ciência e a TecnologiaMountain Research Center - UIDB/00690/2020info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PTUIDB/00690/20206817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaMountain Research Center - UIDP/00690/2020info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PTUIDP/00690/20206817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871other research producthttp://purl.org/coar/resource_type/c_c94fconference object2022http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.unipb.pt/bitstreams/bc4d3a26-22e1-4cf7-8237-a1f65c0019b2/downloadXXVI Encontro Galego-Portugues de Química: Book of Abstracts354354Santiago de Compostela |
| spellingShingle | Study of the antioxidant, antimicrobial and anti-inflammatory activities of two Euphorbia species Perez Vazquez, Ana Euphorbia Research Subject Categories::MEDICINE::Morphology, cell biology, pathology |
| status | SINGLETON |
| subject.fl_str_mv | Euphorbia Research Subject Categories::MEDICINE::Morphology, cell biology, pathology |
| title | Study of the antioxidant, antimicrobial and anti-inflammatory activities of two Euphorbia species |
| title_full | Study of the antioxidant, antimicrobial and anti-inflammatory activities of two Euphorbia species |
| title_fullStr | Study of the antioxidant, antimicrobial and anti-inflammatory activities of two Euphorbia species |
| title_full_unstemmed | Study of the antioxidant, antimicrobial and anti-inflammatory activities of two Euphorbia species |
| title_short | Study of the antioxidant, antimicrobial and anti-inflammatory activities of two Euphorbia species |
| title_sort | Study of the antioxidant, antimicrobial and anti-inflammatory activities of two Euphorbia species |
| topic | Euphorbia Research Subject Categories::MEDICINE::Morphology, cell biology, pathology |
| topic_facet | Euphorbia Research Subject Categories::MEDICINE::Morphology, cell biology, pathology |
| url | http://hdl.handle.net/10198/27812 |
| visible | 1 |