Publicação
The progression from a lower to a higher invasive stage of bladder cancer is associated with severe alterations in glucose and pyruvate metabolism
| Resumo: | Cancer cells present a particular metabolic behavior. We hypothesized that the progression of bladder cancer could be accompanied by changes in cells glycolytic profile. We studied two human bladder cancer cells, RT4 and TCCSUP, in which the latter represents a more invasive stage. The levels of glucose, pyruvate, alanine and lactate in the extracellular media were measured by Proton Nuclear Magnetic Resonance. The protein expression levels of glucose transporters 1 (GLUT1) and 3 (GLUT3), monocarboxylate transporter 4 (MCT4), phosphofructokinase-1 (PFK1), glutamic-pyruvate transaminase (GPT) and lactate dehydrogenase (LDH) were determined. Our data showed that glucose consumption and GLUT3 levels were similar in both cell lines, but TCCSUP cells displayed lower levels of GLUT1 and PFK expression. An increase in pyruvate consumption, concordant with the higher levels of lactate and alanine production, was also detected in TCCSUP cells. Moreover, TCCSUP cells presented lower protein expression levels of GPT and LDH. These results illustrate that bladder cancer progression is associated with alterations in cells glycolytic profile, namely the switch from glucose to pyruvate consumption in the more aggressive stage. This may be useful to develop new therapies and to identify biomarkers for cancer progression. |
|---|---|
| Autores principais: | Conde, Vanessa R. |
| Outros Autores: | Oliveira, Pedro F.; Nunes, Ana R.; Rocha, Cátia S.; Ramalhosa, Elsa; Pereira, J.A.; Alves, Marco G.; Silva, Branca M. |
| Assunto: | Bladder cancer TCCSUP RT4 Metabolism Warburg effect |
| Ano: | 2015 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade Politécnica de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital da UPB |
| _version_ | 1877782387502350336 |
|---|---|
| author | Conde, Vanessa R. |
| author2 | Oliveira, Pedro F. Nunes, Ana R. Rocha, Cátia S. Ramalhosa, Elsa Pereira, J.A. Alves, Marco G. Silva, Branca M. |
| author2_role | author author author author author author author |
| author_facet | Conde, Vanessa R. Oliveira, Pedro F. Nunes, Ana R. Rocha, Cátia S. Ramalhosa, Elsa Pereira, J.A. Alves, Marco G. Silva, Branca M. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital da UPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Conde, Vanessa R.\"},{\"Person.name\":\"Oliveira, Pedro F.\"},{\"Person.name\":\"Nunes, Ana R.\"},{\"Person.name\":\"Rocha, Cátia S.\"},{\"Person.name\":\"Ramalhosa, Elsa\",\"Person.identifier.cienciaID\":\"1A1D-FC05-A05D\",\"Person.identifier.orcid\":\"0000-0003-2503-9705\",\"Person.identifier.scopusAuthorID\":\"6602978189\"},{\"Person.name\":\"Pereira, J.A.\",\"Person.identifier.cienciaID\":\"611F-80B2-A7C1\",\"Person.identifier.orcid\":\"0000-0002-2260-0600\",\"Person.identifier.researcherID\":\"L-6798-2014\",\"Person.identifier.scopusAuthorID\":\"57204366348\"},{\"Person.name\":\"Alves, Marco G.\"},{\"Person.name\":\"Silva, Branca M.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital da UPB |
| datacite.creators.creator.creatorName.fl_str_mv | Conde, Vanessa R. Oliveira, Pedro F. Nunes, Ana R. Rocha, Cátia S. Ramalhosa, Elsa Pereira, J.A. Alves, Marco G. Silva, Branca M. |
| datacite.date.Accepted.fl_str_mv | 2015-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2016-11-16T11:38:06Z |
| datacite.date.embargoed.fl_str_mv | 2016-11-16T11:38:06Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Bladder cancer TCCSUP RT4 Metabolism Warburg effect |
| datacite.titles.title.fl_str_mv | The progression from a lower to a higher invasive stage of bladder cancer is associated with severe alterations in glucose and pyruvate metabolism |
| dc.contributor.none.fl_str_mv | Biblioteca Digital da UPB |
| dc.creator.none.fl_str_mv | Conde, Vanessa R. Oliveira, Pedro F. Nunes, Ana R. Rocha, Cátia S. Ramalhosa, Elsa Pereira, J.A. Alves, Marco G. Silva, Branca M. |
| dc.date.Accepted.fl_str_mv | 2015-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2016-11-16T11:38:06Z |
| dc.date.embargoed.fl_str_mv | 2016-11-16T11:38:06Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/13500 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Bladder cancer TCCSUP RT4 Metabolism Warburg effect |
| dc.title.fl_str_mv | The progression from a lower to a higher invasive stage of bladder cancer is associated with severe alterations in glucose and pyruvate metabolism |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Cancer cells present a particular metabolic behavior. We hypothesized that the progression of bladder cancer could be accompanied by changes in cells glycolytic profile. We studied two human bladder cancer cells, RT4 and TCCSUP, in which the latter represents a more invasive stage. The levels of glucose, pyruvate, alanine and lactate in the extracellular media were measured by Proton Nuclear Magnetic Resonance. The protein expression levels of glucose transporters 1 (GLUT1) and 3 (GLUT3), monocarboxylate transporter 4 (MCT4), phosphofructokinase-1 (PFK1), glutamic-pyruvate transaminase (GPT) and lactate dehydrogenase (LDH) were determined. Our data showed that glucose consumption and GLUT3 levels were similar in both cell lines, but TCCSUP cells displayed lower levels of GLUT1 and PFK expression. An increase in pyruvate consumption, concordant with the higher levels of lactate and alanine production, was also detected in TCCSUP cells. Moreover, TCCSUP cells presented lower protein expression levels of GPT and LDH. These results illustrate that bladder cancer progression is associated with alterations in cells glycolytic profile, namely the switch from glucose to pyruvate consumption in the more aggressive stage. This may be useful to develop new therapies and to identify biomarkers for cancer progression. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.unipb.pt/bitstreams/81052e61-b6e3-466c-a32d-62b818838f8d/download |
| funder_facet_str_mv | FCT{{{_:::_}}}Fundação para a Ciência e a Tecnologia FCT{{{_:::_}}}Fundação para a Ciência e a Tecnologia FCT{{{_:::_}}}Fundação para a Ciência e a Tecnologia |
| funding.funder.alternateName_str_mv | FCT FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.identifier_str_mv | PTDC/QUI-BIQ/121446/2010 PEst-OE/SAU/UI0709/2014 SFRH/BPD/80451/2011 |
| funding.name_str_mv | 5876-PPCDTI 5876 SFRH |
| funding_str_mv | PTDC/QUI-BIQ/121446/2010 info:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FQUI-BIQ%2F121446%2F2010/PT PEst-OE/SAU/UI0709/2014 info:eu-repo/grantAgreement/FCT/5876/PEst-OE%2FSAU%2FUI0709%2F2014/PT SFRH/BPD/80451/2011 info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F80451%2F2011/PT |
| id | ipb_b7d4eaaacae1d2611f4f76d2f4620fae |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/13500 |
| inst_facet_str | urn:organizationAcronym:unipb{{{_:::_}}}Universidade Politécnica de Bragança |
| instacron_str | unipb |
| institution | Universidade Politécnica de Bragança |
| instname_str | Universidade Politécnica de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital da UPB |
| oai_identifier_str | oai:bibliotecadigital.unipb.pt:10198/13500 |
| organization_str_mv | urn:organizationAcronym:unipb |
| person_str_mv | Conde, Vanessa R. Oliveira, Pedro F. Nunes, Ana R. Rocha, Cátia S. Ramalhosa, Elsa Ramalhosa, Elsa https://www.ciencia-id.pt/1A1D-FC05-A05D 1A1D-FC05-A05D http://orcid.org/0000-0003-2503-9705 0000-0003-2503-9705 Pereira, J.A. Pereira, J.A. https://www.ciencia-id.pt/611F-80B2-A7C1 611F-80B2-A7C1 http://orcid.org/0000-0002-2260-0600 0000-0002-2260-0600 Alves, Marco G. Silva, Branca M. |
| publishDate | 2015 |
| publisher.none.fl_str_mv | Elsevier |
| repo_facet_str | urn:repositoryAcronym:ipb{{{_:::_}}}Biblioteca Digital da UPB |
| reponame_str | Biblioteca Digital da UPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engElsevierpt_PTCancer cells present a particular metabolic behavior. We hypothesized that the progression of bladder cancer could be accompanied by changes in cells glycolytic profile. We studied two human bladder cancer cells, RT4 and TCCSUP, in which the latter represents a more invasive stage. The levels of glucose, pyruvate, alanine and lactate in the extracellular media were measured by Proton Nuclear Magnetic Resonance. The protein expression levels of glucose transporters 1 (GLUT1) and 3 (GLUT3), monocarboxylate transporter 4 (MCT4), phosphofructokinase-1 (PFK1), glutamic-pyruvate transaminase (GPT) and lactate dehydrogenase (LDH) were determined. Our data showed that glucose consumption and GLUT3 levels were similar in both cell lines, but TCCSUP cells displayed lower levels of GLUT1 and PFK expression. An increase in pyruvate consumption, concordant with the higher levels of lactate and alanine production, was also detected in TCCSUP cells. Moreover, TCCSUP cells presented lower protein expression levels of GPT and LDH. These results illustrate that bladder cancer progression is associated with alterations in cells glycolytic profile, namely the switch from glucose to pyruvate consumption in the more aggressive stage. This may be useful to develop new therapies and to identify biomarkers for cancer progression.application/pdfpt_PTThe progression from a lower to a higher invasive stage of bladder cancer is associated with severe alterations in glucose and pyruvate metabolismConde, Vanessa R.Oliveira, Pedro F.Nunes, Ana R.Rocha, Cátia S.PersonalRamalhosa, ElsaDSpacehttp://dspace.org/items/d74be6c0-1703-4f77-bd81-e289ec981ee3DSpacehttp://dspace.org/items/d74be6c0-1703-4f77-bd81-e289ec981ee3RamalhosaElsaCiência IDhttps://www.ciencia-id.pt1A1D-FC05-A05DORCIDhttp://orcid.org0000-0003-2503-9705Scopus Author IDhttps://www.scopus.com6602978189PersonalPereira, J.A.DSpacehttp://dspace.org/items/7932162e-a2da-4913-b00d-17babbe51857DSpacehttp://dspace.org/items/7932162e-a2da-4913-b00d-17babbe51857PereiraJosé AlbertoCiência IDhttps://www.ciencia-id.pt611F-80B2-A7C1ORCIDhttp://orcid.org0000-0002-2260-0600Researcher IDhttps://www.researcherid.comL-6798-2014Scopus Author IDhttps://www.scopus.com57204366348Alves, Marco G.Silva, Branca M.HostingInstitutionOrganizationalBiblioteca Digital da UPBe-mailmailto:dspace@unipb.ptdspace@unipb.ptISSNIsPartOf0014-4827DOIIsPartOf10.1016/j.yexcr.2015.04.0072016-11-16T11:38:06Z20152015-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/13500http://purl.org/coar/access_right/c_16ecrestricted accessBladder cancerTCCSUPRT4MetabolismWarburg effect1042022 bytesFundação para a Ciência e a TecnologiaCan Hormonal (De)regulation of Ion Transporters in Human Sertoli Cells be Responsible for Infertility?info:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FQUI-BIQ%2F121446%2F2010/PTPTDC/QUI-BIQ/121446/20105876-PPCDTICrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaPEst-OE/SAU/UI0709/2014info:eu-repo/grantAgreement/FCT/5876/PEst-OE%2FSAU%2FUI0709%2F2014/PTPEst-OE/SAU/UI0709/20145876Crossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaType 2 diabetes mellitus and male infertility: unraveling the metabolic dysfunction in testes and sertoli cellsinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F80451%2F2011/PTSFRH/BPD/80451/2011SFRHCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal article2015http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://bibliotecadigital.unipb.pt/bitstreams/81052e61-b6e3-466c-a32d-62b818838f8d/downloadExperimental Cell Research33519198 |
| spellingShingle | The progression from a lower to a higher invasive stage of bladder cancer is associated with severe alterations in glucose and pyruvate metabolism Conde, Vanessa R. Bladder cancer TCCSUP RT4 Metabolism Warburg effect |
| status | SINGLETON |
| subject.fl_str_mv | Bladder cancer TCCSUP RT4 Metabolism Warburg effect |
| title | The progression from a lower to a higher invasive stage of bladder cancer is associated with severe alterations in glucose and pyruvate metabolism |
| title_full | The progression from a lower to a higher invasive stage of bladder cancer is associated with severe alterations in glucose and pyruvate metabolism |
| title_fullStr | The progression from a lower to a higher invasive stage of bladder cancer is associated with severe alterations in glucose and pyruvate metabolism |
| title_full_unstemmed | The progression from a lower to a higher invasive stage of bladder cancer is associated with severe alterations in glucose and pyruvate metabolism |
| title_short | The progression from a lower to a higher invasive stage of bladder cancer is associated with severe alterations in glucose and pyruvate metabolism |
| title_sort | The progression from a lower to a higher invasive stage of bladder cancer is associated with severe alterations in glucose and pyruvate metabolism |
| topic | Bladder cancer TCCSUP RT4 Metabolism Warburg effect |
| topic_facet | Bladder cancer TCCSUP RT4 Metabolism Warburg effect |
| url | http://hdl.handle.net/10198/13500 |
| visible | 1 |