Publication
HPLC-DAD-(ESI)-MS/MS analysis as the first step to metabolic fingerprinting of medicinal herbs: the case of underexploited Euphorbia species
| Summary: | The genus Euphorbia comprises more than 2,000 species widely distributed in Asia, Africa and Latin America. They have been extensively used in folk medicine to treat disorders such as abdominal pain, skin diseases, tumors, wart, among others¹. Although the well-recognized ethnopharmacological relevance of Euphorbia species, most of them have not been studied yet, such as Euphorbia hirta and Euphorbia jokinii. In this context, screening the bioactive molecules potentially responsible for the observed medicinal effects is an initial, but key step for discovering novel active compounds and understanding the mechanisms of action underlying their biological activities. Thus, this study aimed at determining the phenolic compounds composition of E. Hirta and E. jokinii. Both plants were freezedried, milled, and extracted with ethanol:water (60:40 v/v) for 3 hours at 45 °C. After centrifugation, the extracts were freeze-dried, re-suspended in ethanol:water (20:80 v/v), filtered and injected into the HPLC-DAD-(ESI)-MS/MS system. The phenolic profile of E. Hirta and E. Jokinii revealed mainly the presence of flavonoids and galloyl derivatives. Thirty-two compounds were tentatively identified in E. Hirta and twenty-two in E. Jokinii. Quercetin 3-O-rhamnoside was the major compound in both species, with E. Hirta showing the highest content (10.5 mg/g vs 8.2 mg/g extract). Our results indicate that E. Hirta and E. Jokinii, yet underexploited Euphorbia species, are interesting sources of flavonoids. Further studies should evaluate the relationship between these compounds and the biological activities of these plants, as well as establish possible related mechanisms of action. |
|---|---|
| Main Authors: | Silveira, Tayse F. F. da |
| Other Authors: | Rodrigues, Daniele Bobrowski; Vazquez, A.P.; Alvarez, Paula Barciela; Carpena Rodríguez, María; Simal-Gandara, Jesus; Prieto Lage, Miguel A.; Barros, Lillian |
| Subject: | Metabolic fingerprinting Medicinal herbs Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology |
| Year: | 2022 |
| Country: | Portugal |
| Document type: | conference output |
| Access type: | open access |
| Associated institution: | Universidade Politécnica de Bragança |
| Language: | English |
| Origin: | Biblioteca Digital da UPB |
| _version_ | 1877782725868388352 |
|---|---|
| author | Silveira, Tayse F. F. da |
| author2 | Rodrigues, Daniele Bobrowski Vazquez, A.P. Alvarez, Paula Barciela Carpena Rodríguez, María Simal-Gandara, Jesus Prieto Lage, Miguel A. Barros, Lillian |
| author2_role | author author author author author author author |
| author_facet | Silveira, Tayse F. F. da Rodrigues, Daniele Bobrowski Vazquez, A.P. Alvarez, Paula Barciela Carpena Rodríguez, María Simal-Gandara, Jesus Prieto Lage, Miguel A. Barros, Lillian |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital da UPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Silveira, Tayse F. F. da\",\"Person.identifier.cienciaID\":\"541E-97D4-A3E5\"},{\"Person.name\":\"Rodrigues, Daniele Bobrowski\",\"Person.identifier.cienciaID\":\"A91B-FE1D-B275\",\"Person.identifier.orcid\":\"0000-0003-0260-5751\"},{\"Person.name\":\"Vazquez, A.P.\"},{\"Person.name\":\"Alvarez, Paula Barciela\"},{\"Person.name\":\"Carpena Rodríguez, María\"},{\"Person.name\":\"Simal-Gandara, Jesus\"},{\"Person.name\":\"Prieto Lage, Miguel A.\",\"Person.identifier.cienciaID\":\"221E-1E00-AB74\",\"Person.identifier.orcid\":\"0000-0002-3513-0054\",\"Person.identifier.researcherID\":\"G-4516-2011\",\"Person.identifier.scopusAuthorID\":\"35937495700\",\"Person.identifier.scopusAuthorID\":\"57207755963\"},{\"Person.name\":\"Barros, Lillian\",\"Person.identifier.cienciaID\":\"9616-35CB-D001\",\"Person.identifier.orcid\":\"0000-0002-9050-5189\",\"Person.identifier.researcherID\":\"J-3600-2013\",\"Person.identifier.researcherID\":\"J-3600-2013\",\"Person.identifier.scopusAuthorID\":\"35236343600\",\"Person.identifier.scopusAuthorID\":\"57194900801\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital da UPB |
| datacite.creators.creator.creatorName.fl_str_mv | Silveira, Tayse F. F. da Rodrigues, Daniele Bobrowski Vazquez, A.P. Alvarez, Paula Barciela Carpena Rodríguez, María Simal-Gandara, Jesus Prieto Lage, Miguel A. Barros, Lillian |
| datacite.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2023-03-17T14:01:58Z |
| datacite.date.embargoed.fl_str_mv | 2023-03-17T14:01:58Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Metabolic fingerprinting Medicinal herbs Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology |
| datacite.titles.title.fl_str_mv | HPLC-DAD-(ESI)-MS/MS analysis as the first step to metabolic fingerprinting of medicinal herbs: the case of underexploited Euphorbia species |
| dc.contributor.none.fl_str_mv | Biblioteca Digital da UPB |
| dc.creator.none.fl_str_mv | Silveira, Tayse F. F. da Rodrigues, Daniele Bobrowski Vazquez, A.P. Alvarez, Paula Barciela Carpena Rodríguez, María Simal-Gandara, Jesus Prieto Lage, Miguel A. Barros, Lillian |
| dc.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2023-03-17T14:01:58Z |
| dc.date.embargoed.fl_str_mv | 2023-03-17T14:01:58Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/27811 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Sociedade Portuguesa de Química |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Metabolic fingerprinting Medicinal herbs Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology |
| dc.title.fl_str_mv | HPLC-DAD-(ESI)-MS/MS analysis as the first step to metabolic fingerprinting of medicinal herbs: the case of underexploited Euphorbia species |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | The genus Euphorbia comprises more than 2,000 species widely distributed in Asia, Africa and Latin America. They have been extensively used in folk medicine to treat disorders such as abdominal pain, skin diseases, tumors, wart, among others¹. Although the well-recognized ethnopharmacological relevance of Euphorbia species, most of them have not been studied yet, such as Euphorbia hirta and Euphorbia jokinii. In this context, screening the bioactive molecules potentially responsible for the observed medicinal effects is an initial, but key step for discovering novel active compounds and understanding the mechanisms of action underlying their biological activities. Thus, this study aimed at determining the phenolic compounds composition of E. Hirta and E. jokinii. Both plants were freezedried, milled, and extracted with ethanol:water (60:40 v/v) for 3 hours at 45 °C. After centrifugation, the extracts were freeze-dried, re-suspended in ethanol:water (20:80 v/v), filtered and injected into the HPLC-DAD-(ESI)-MS/MS system. The phenolic profile of E. Hirta and E. Jokinii revealed mainly the presence of flavonoids and galloyl derivatives. Thirty-two compounds were tentatively identified in E. Hirta and twenty-two in E. Jokinii. Quercetin 3-O-rhamnoside was the major compound in both species, with E. Hirta showing the highest content (10.5 mg/g vs 8.2 mg/g extract). Our results indicate that E. Hirta and E. Jokinii, yet underexploited Euphorbia species, are interesting sources of flavonoids. Further studies should evaluate the relationship between these compounds and the biological activities of these plants, as well as establish possible related mechanisms of action. |
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| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.identifier_str_mv | UIDB/00690/2020 UIDP/00690/2020 |
| funding.name_str_mv | 6817 - DCRRNI ID 6817 - DCRRNI ID |
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| person_str_mv | Silveira, Tayse F. F. da Silveira, Tayse F. F. da https://www.ciencia-id.pt/541E-97D4-A3E5 541E-97D4-A3E5 Rodrigues, Daniele Bobrowski Rodrigues, Daniele Bobrowski https://www.ciencia-id.pt/A91B-FE1D-B275 A91B-FE1D-B275 http://orcid.org/0000-0003-0260-5751 0000-0003-0260-5751 Vazquez, A.P. Alvarez, Paula Barciela Carpena Rodríguez, María Simal-Gandara, Jesus Prieto Lage, Miguel A. Prieto Lage, Miguel A. https://www.ciencia-id.pt/221E-1E00-AB74 221E-1E00-AB74 http://orcid.org/0000-0002-3513-0054 0000-0002-3513-0054 Barros, Lillian Barros, Lillian https://www.ciencia-id.pt/9616-35CB-D001 9616-35CB-D001 http://orcid.org/0000-0002-9050-5189 0000-0002-9050-5189 |
| publishDate | 2022 |
| publisher.none.fl_str_mv | Sociedade Portuguesa de Química |
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| spelling | engSociedade Portuguesa de Químicapt_PTThe genus Euphorbia comprises more than 2,000 species widely distributed in Asia, Africa and Latin America. They have been extensively used in folk medicine to treat disorders such as abdominal pain, skin diseases, tumors, wart, among others¹. Although the well-recognized ethnopharmacological relevance of Euphorbia species, most of them have not been studied yet, such as Euphorbia hirta and Euphorbia jokinii. In this context, screening the bioactive molecules potentially responsible for the observed medicinal effects is an initial, but key step for discovering novel active compounds and understanding the mechanisms of action underlying their biological activities. Thus, this study aimed at determining the phenolic compounds composition of E. Hirta and E. jokinii. Both plants were freezedried, milled, and extracted with ethanol:water (60:40 v/v) for 3 hours at 45 °C. After centrifugation, the extracts were freeze-dried, re-suspended in ethanol:water (20:80 v/v), filtered and injected into the HPLC-DAD-(ESI)-MS/MS system. The phenolic profile of E. Hirta and E. Jokinii revealed mainly the presence of flavonoids and galloyl derivatives. Thirty-two compounds were tentatively identified in E. Hirta and twenty-two in E. Jokinii. Quercetin 3-O-rhamnoside was the major compound in both species, with E. Hirta showing the highest content (10.5 mg/g vs 8.2 mg/g extract). Our results indicate that E. Hirta and E. Jokinii, yet underexploited Euphorbia species, are interesting sources of flavonoids. Further studies should evaluate the relationship between these compounds and the biological activities of these plants, as well as establish possible related mechanisms of action.application/pdfpt_PTHPLC-DAD-(ESI)-MS/MS analysis as the first step to metabolic fingerprinting of medicinal herbs: the case of underexploited Euphorbia speciesPersonalSilveira, Tayse F. F. daDSpacehttp://dspace.org/items/6b60de6a-591c-4a30-95ea-cc5feebe8a30DSpacehttp://dspace.org/items/6b60de6a-591c-4a30-95ea-cc5feebe8a30SilveiraTayse F. F. daCiência IDhttps://www.ciencia-id.pt541E-97D4-A3E5PersonalRodrigues, Daniele BobrowskiDSpacehttp://dspace.org/items/18a661e9-0f77-4bd1-b2b0-5b929b760ab3DSpacehttp://dspace.org/items/18a661e9-0f77-4bd1-b2b0-5b929b760ab3RodriguesDaniele BobrowskiCiência IDhttps://www.ciencia-id.ptA91B-FE1D-B275ORCIDhttp://orcid.org0000-0003-0260-5751Vazquez, A.P.Alvarez, Paula BarcielaCarpena Rodríguez, MaríaSimal-Gandara, JesusPersonalPrieto Lage, Miguel A.DSpacehttp://dspace.org/items/13260615-fd28-4b8b-9dd4-8a8466007579DSpacehttp://dspace.org/items/13260615-fd28-4b8b-9dd4-8a8466007579PrietoMiguel A.Ciência IDhttps://www.ciencia-id.pt221E-1E00-AB74ORCIDhttp://orcid.org0000-0002-3513-0054Researcher IDhttps://www.researcherid.comG-4516-2011Scopus Author IDhttps://www.scopus.com35937495700Scopus Author IDhttps://www.scopus.com57207755963PersonalBarros, LillianDSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01DSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01BarrosLillianCiência IDhttps://www.ciencia-id.pt9616-35CB-D001ORCIDhttp://orcid.org0000-0002-9050-5189Researcher IDhttps://www.researcherid.comJ-3600-2013Researcher IDhttps://www.researcherid.comJ-3600-2013Scopus Author IDhttps://www.scopus.com35236343600Scopus Author IDhttps://www.scopus.com57194900801HostingInstitutionOrganizationalBiblioteca Digital da UPBe-mailmailto:dspace@unipb.ptdspace@unipb.ptISBNIsPartOf978-989-8124-37-12023-03-17T14:01:58Z20222022-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/27811http://purl.org/coar/access_right/c_abf2open accessMetabolic fingerprintingMedicinal herbsResearch Subject Categories::TECHNOLOGY::Chemical engineering::Food technology319960 bytesFundação para a Ciência e a TecnologiaMountain Research Center - UIDB/00690/2020info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PTUIDB/00690/20206817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaMountain Research Center - UIDP/00690/2020info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PTUIDP/00690/20206817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871other research producthttp://purl.org/coar/resource_type/c_c94fconference object2022http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.unipb.pt/bitstreams/5d463cfe-6033-46f5-b907-b447714b5f25/downloadLivro de Resumos do 12º Encontro Nacional de Cromatografia & XIV WARPA195195Aveiro |
| spellingShingle | HPLC-DAD-(ESI)-MS/MS analysis as the first step to metabolic fingerprinting of medicinal herbs: the case of underexploited Euphorbia species Silveira, Tayse F. F. da Metabolic fingerprinting Medicinal herbs Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology |
| status | SINGLETON |
| subject.fl_str_mv | Metabolic fingerprinting Medicinal herbs Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology |
| title | HPLC-DAD-(ESI)-MS/MS analysis as the first step to metabolic fingerprinting of medicinal herbs: the case of underexploited Euphorbia species |
| title_full | HPLC-DAD-(ESI)-MS/MS analysis as the first step to metabolic fingerprinting of medicinal herbs: the case of underexploited Euphorbia species |
| title_fullStr | HPLC-DAD-(ESI)-MS/MS analysis as the first step to metabolic fingerprinting of medicinal herbs: the case of underexploited Euphorbia species |
| title_full_unstemmed | HPLC-DAD-(ESI)-MS/MS analysis as the first step to metabolic fingerprinting of medicinal herbs: the case of underexploited Euphorbia species |
| title_short | HPLC-DAD-(ESI)-MS/MS analysis as the first step to metabolic fingerprinting of medicinal herbs: the case of underexploited Euphorbia species |
| title_sort | HPLC-DAD-(ESI)-MS/MS analysis as the first step to metabolic fingerprinting of medicinal herbs: the case of underexploited Euphorbia species |
| topic | Metabolic fingerprinting Medicinal herbs Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology |
| topic_facet | Metabolic fingerprinting Medicinal herbs Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology |
| url | http://hdl.handle.net/10198/27811 |
| visible | 1 |