Publicação
Wild mushroom extracts as inhibitors of bacterial biofilm formation
| Resumo: | Introduction: Microorganisms can colonize a wide variety of medical devices, putting patients in risk for local and systemic infectious complications. These microorganisms are able to grow adhered to almost every surface, forming biofilms. The use of natural products has been successful in the discovery of new medicine, and mushrooms could be a source of natural antimicrobials. Objectives: The present study reports the capacity of wild mushroom extracts to inhibit in vitro biofilm formation by multi-resistant bacteria. Methods: Four Gram-negative bacteria biofilm producers (Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa and Acinetobacter baumannii) isolated from urine were used to verify the activity of Russula delica, Fistulina hepatica, Mycena rosea, Leucopaxilus giganteus and Lepista nuda extracts. Results: The results obtained showed that all tested mushroom extracts presented some extent of inhibition of biofilm production. Pseudomonas aeruginosa was the microorganism with the highest capacity of biofilm production, being also the most susceptible to the extracts inhibition capacity (≥ 50%). Among the five tested extracts against E. coli, Leucopaxillus giganteus (47.8%) and Mycenas rosea (44.8%) presented the highest inhibition of bio lm formation. The extracts exhibiting the highest inhibitory effect upon P. mirabilis biofilm formation were Sarcodon imbricatus (45.4%) and Russula delica (53.1%). Acinetobacter baumannii was the microorganism with the lowest susceptibility to mushroom extracts inhibitory effect on biofilm production. Conclusions: This is a pioneer study since, as far as we know, there are no reports on the inhibition of biofilm production by the studied mushroom extracts and in particular against multi-resistant clinical isolates. |
|---|---|
| Autores principais: | Alves, Maria José |
| Outros Autores: | Ferreira, Isabel C.F.R.; Lourenço, Inês; Costa, Eduardo Manuel; Martins, Anabela; Pintado, Manuela |
| Assunto: | Clinical isolates Biofilm Mushroom extracts |
| Ano: | 2014 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Politécnica de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital da UPB |
| _version_ | 1877782528526385152 |
|---|---|
| author | Alves, Maria José |
| author2 | Ferreira, Isabel C.F.R. Lourenço, Inês Costa, Eduardo Manuel Martins, Anabela Pintado, Manuela |
| author2_role | author author author author author |
| author_facet | Alves, Maria José Ferreira, Isabel C.F.R. Lourenço, Inês Costa, Eduardo Manuel Martins, Anabela Pintado, Manuela |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital da UPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Alves, Maria José\",\"Person.identifier.cienciaID\":\"D618-B3A9-D312\",\"Person.identifier.orcid\":\"0000-0001-8506-4393\",\"Person.identifier.researcherID\":\"M-6624-2016\",\"Person.identifier.scopusAuthorID\":\"16400584800\"},{\"Person.name\":\"Ferreira, Isabel C.F.R.\",\"Person.identifier.cienciaID\":\"9418-CF95-9919\",\"Person.identifier.orcid\":\"0000-0003-4910-4882\",\"Person.identifier.researcherID\":\"E-8500-2013\",\"Person.identifier.scopusAuthorID\":\"36868826600\"},{\"Person.name\":\"Lourenço, Inês\"},{\"Person.name\":\"Costa, Eduardo Manuel\"},{\"Person.name\":\"Martins, Anabela\",\"Person.identifier.cienciaID\":\"7B18-A810-6B93\",\"Person.identifier.orcid\":\"0000-0001-6218-4413\",\"Person.identifier.researcherID\":\"G-5488-2013\",\"Person.identifier.scopusAuthorID\":\"7203013518\"},{\"Person.name\":\"Pintado, Manuela\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital da UPB |
| datacite.creators.creator.creatorName.fl_str_mv | Alves, Maria José Ferreira, Isabel C.F.R. Lourenço, Inês Costa, Eduardo Manuel Martins, Anabela Pintado, Manuela |
| datacite.date.Accepted.fl_str_mv | 2014-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2015-10-29T11:40:02Z |
| datacite.date.embargoed.fl_str_mv | 2015-10-29T11:40:02Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Clinical isolates Biofilm Mushroom extracts |
| datacite.titles.title.fl_str_mv | Wild mushroom extracts as inhibitors of bacterial biofilm formation |
| dc.contributor.none.fl_str_mv | Biblioteca Digital da UPB |
| dc.creator.none.fl_str_mv | Alves, Maria José Ferreira, Isabel C.F.R. Lourenço, Inês Costa, Eduardo Manuel Martins, Anabela Pintado, Manuela |
| dc.date.Accepted.fl_str_mv | 2014-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2015-10-29T11:40:02Z |
| dc.date.embargoed.fl_str_mv | 2015-10-29T11:40:02Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/12220 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Clinical isolates Biofilm Mushroom extracts |
| dc.title.fl_str_mv | Wild mushroom extracts as inhibitors of bacterial biofilm formation |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | Introduction: Microorganisms can colonize a wide variety of medical devices, putting patients in risk for local and systemic infectious complications. These microorganisms are able to grow adhered to almost every surface, forming biofilms. The use of natural products has been successful in the discovery of new medicine, and mushrooms could be a source of natural antimicrobials. Objectives: The present study reports the capacity of wild mushroom extracts to inhibit in vitro biofilm formation by multi-resistant bacteria. Methods: Four Gram-negative bacteria biofilm producers (Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa and Acinetobacter baumannii) isolated from urine were used to verify the activity of Russula delica, Fistulina hepatica, Mycena rosea, Leucopaxilus giganteus and Lepista nuda extracts. Results: The results obtained showed that all tested mushroom extracts presented some extent of inhibition of biofilm production. Pseudomonas aeruginosa was the microorganism with the highest capacity of biofilm production, being also the most susceptible to the extracts inhibition capacity (≥ 50%). Among the five tested extracts against E. coli, Leucopaxillus giganteus (47.8%) and Mycenas rosea (44.8%) presented the highest inhibition of bio lm formation. The extracts exhibiting the highest inhibitory effect upon P. mirabilis biofilm formation were Sarcodon imbricatus (45.4%) and Russula delica (53.1%). Acinetobacter baumannii was the microorganism with the lowest susceptibility to mushroom extracts inhibitory effect on biofilm production. Conclusions: This is a pioneer study since, as far as we know, there are no reports on the inhibition of biofilm production by the studied mushroom extracts and in particular against multi-resistant clinical isolates. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | conferenceObject |
| fulltext.url.fl_str_mv | https://bibliotecadigital.unipb.pt/bitstreams/0bc724f2-0deb-4e61-9169-8c407a59442b/download |
| id | ipb_8db36aabd0854fdfe4bde1cbba5cdac2 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/12220 |
| inst_facet_str | urn:organizationAcronym:unipb{{{_:::_}}}Universidade Politécnica de Bragança |
| instacron_str | unipb |
| institution | Universidade Politécnica de Bragança |
| instname_str | Universidade Politécnica de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital da UPB |
| oai_identifier_str | oai:bibliotecadigital.unipb.pt:10198/12220 |
| organization_str_mv | urn:organizationAcronym:unipb |
| person_str_mv | Alves, Maria José Alves, Maria José https://www.ciencia-id.pt/D618-B3A9-D312 D618-B3A9-D312 http://orcid.org/0000-0001-8506-4393 0000-0001-8506-4393 Ferreira, Isabel C.F.R. Ferreira, Isabel C.F.R. https://www.ciencia-id.pt/9418-CF95-9919 9418-CF95-9919 http://orcid.org/0000-0003-4910-4882 0000-0003-4910-4882 Lourenço, Inês Costa, Eduardo Manuel Martins, Anabela Martins, Anabela https://www.ciencia-id.pt/7B18-A810-6B93 7B18-A810-6B93 http://orcid.org/0000-0001-6218-4413 0000-0001-6218-4413 Pintado, Manuela |
| publishDate | 2014 |
| repo_facet_str | urn:repositoryAcronym:ipb{{{_:::_}}}Biblioteca Digital da UPB |
| reponame_str | Biblioteca Digital da UPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engpt_PTIntroduction: Microorganisms can colonize a wide variety of medical devices, putting patients in risk for local and systemic infectious complications. These microorganisms are able to grow adhered to almost every surface, forming biofilms. The use of natural products has been successful in the discovery of new medicine, and mushrooms could be a source of natural antimicrobials. Objectives: The present study reports the capacity of wild mushroom extracts to inhibit in vitro biofilm formation by multi-resistant bacteria. Methods: Four Gram-negative bacteria biofilm producers (Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa and Acinetobacter baumannii) isolated from urine were used to verify the activity of Russula delica, Fistulina hepatica, Mycena rosea, Leucopaxilus giganteus and Lepista nuda extracts. Results: The results obtained showed that all tested mushroom extracts presented some extent of inhibition of biofilm production. Pseudomonas aeruginosa was the microorganism with the highest capacity of biofilm production, being also the most susceptible to the extracts inhibition capacity (≥ 50%). Among the five tested extracts against E. coli, Leucopaxillus giganteus (47.8%) and Mycenas rosea (44.8%) presented the highest inhibition of bio lm formation. The extracts exhibiting the highest inhibitory effect upon P. mirabilis biofilm formation were Sarcodon imbricatus (45.4%) and Russula delica (53.1%). Acinetobacter baumannii was the microorganism with the lowest susceptibility to mushroom extracts inhibitory effect on biofilm production. Conclusions: This is a pioneer study since, as far as we know, there are no reports on the inhibition of biofilm production by the studied mushroom extracts and in particular against multi-resistant clinical isolates.application/pdfpt_PTWild mushroom extracts as inhibitors of bacterial biofilm formationPersonalAlves, Maria JoséDSpacehttp://dspace.org/items/7850f8c2-8834-4d5a-b4a7-df482abd76d3DSpacehttp://dspace.org/items/7850f8c2-8834-4d5a-b4a7-df482abd76d3AlvesMaria JoséCiência IDhttps://www.ciencia-id.ptD618-B3A9-D312ORCIDhttp://orcid.org0000-0001-8506-4393Researcher IDhttps://www.researcherid.comM-6624-2016Scopus Author IDhttps://www.scopus.com16400584800PersonalFerreira, Isabel C.F.R.DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8FerreiraIsabel C.F.R.Ciência IDhttps://www.ciencia-id.pt9418-CF95-9919ORCIDhttp://orcid.org0000-0003-4910-4882Researcher IDhttps://www.researcherid.comE-8500-2013Scopus Author IDhttps://www.scopus.com36868826600Lourenço, InêsCosta, Eduardo ManuelPersonalMartins, AnabelaDSpacehttp://dspace.org/items/383370dd-10e5-40e6-9a15-f2201fe95581DSpacehttp://dspace.org/items/383370dd-10e5-40e6-9a15-f2201fe95581MartinsAnabelaCiência IDhttps://www.ciencia-id.pt7B18-A810-6B93ORCIDhttp://orcid.org0000-0001-6218-4413Researcher IDhttps://www.researcherid.comG-5488-2013Scopus Author IDhttps://www.scopus.com7203013518Pintado, ManuelaHostingInstitutionOrganizationalBiblioteca Digital da UPBe-mailmailto:dspace@unipb.ptdspace@unipb.pt2015-10-29T11:40:02Z20142014-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/12220http://purl.org/coar/access_right/c_abf2open accessClinical isolatesBiofilmMushroom extracts171313 bytesother research producthttp://purl.org/coar/resource_type/c_c94fconference object2014http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.unipb.pt/bitstreams/0bc724f2-0deb-4e61-9169-8c407a59442b/download2nd World Congress of Health Research. Viseu- Portugal, 7-8 October 2014. Aten Primaria. 2014;46(Espec Cong 1):51 |
| spellingShingle | Wild mushroom extracts as inhibitors of bacterial biofilm formation Alves, Maria José Clinical isolates Biofilm Mushroom extracts |
| status | SINGLETON |
| subject.fl_str_mv | Clinical isolates Biofilm Mushroom extracts |
| title | Wild mushroom extracts as inhibitors of bacterial biofilm formation |
| title_full | Wild mushroom extracts as inhibitors of bacterial biofilm formation |
| title_fullStr | Wild mushroom extracts as inhibitors of bacterial biofilm formation |
| title_full_unstemmed | Wild mushroom extracts as inhibitors of bacterial biofilm formation |
| title_short | Wild mushroom extracts as inhibitors of bacterial biofilm formation |
| title_sort | Wild mushroom extracts as inhibitors of bacterial biofilm formation |
| topic | Clinical isolates Biofilm Mushroom extracts |
| topic_facet | Clinical isolates Biofilm Mushroom extracts |
| url | http://hdl.handle.net/10198/12220 |
| visible | 1 |
Atividades financiadas
Não foram encontrados projetos financiados.