Publicação
Visualization and measurement of red blood cells flowing in microfluidic devices
| Resumo: | Several experimental techniques were performed in the past years using in vitro environments, in an attempt to not only understand the blood flow behaviour in microcirculation but also develop microfluidic devices as an alternative clinical methodology to detect blood diseases. Hence, the visualization and measurement of red blood cells (RBCs) flowing in a microfluidic device are important to provide not only essential information about hydrodynamic characteristics of the blood but also vital information to diagnose the initial symptoms of diseases during clinical investigations. For instance, RBC rigidity has been correlated with myocardial infarction, diabetes mellitus, hypertension, and also other haematological disorders and diseases that affect RBC deformation more directly, such as, hereditary spherocytosis, sickle cell anaemia and malaria. Regarding a better understanding of the RBCs deformation and motion, we present in this paper a compilation of studies made in our research group, using several microfluidic devices with different microchannel geometries and fabrication techniques (i.e., soft-lithography, xurography and hybrids) that focus in the shear and extensional flow behaviour, either in healthy or chemically stiffed RBCs. |
|---|---|
| Autores principais: | Rodrigues, Raquel Oliveira |
| Outros Autores: | Pinho, Diana; Faustino, Vera; Yaginuma, Tomoko; Bento, David; Fernandes, Carla S.; Garcia, Valdemar; Lima, Rui A. |
| Assunto: | Red blood cells deformation Microfluidic devices |
| Ano: | 2014 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Politécnica de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital da UPB |
| _version_ | 1877782695539376128 |
|---|---|
| author | Rodrigues, Raquel Oliveira |
| author2 | Pinho, Diana Faustino, Vera Yaginuma, Tomoko Bento, David Fernandes, Carla S. Garcia, Valdemar Lima, Rui A. |
| author2_role | author author author author author author author |
| author_facet | Rodrigues, Raquel Oliveira Pinho, Diana Faustino, Vera Yaginuma, Tomoko Bento, David Fernandes, Carla S. Garcia, Valdemar Lima, Rui A. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital da UPB |
| country_str | PT |
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| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital da UPB |
| datacite.creators.creator.creatorName.fl_str_mv | Rodrigues, Raquel Oliveira Pinho, Diana Faustino, Vera Yaginuma, Tomoko Bento, David Fernandes, Carla S. Garcia, Valdemar Lima, Rui A. |
| datacite.date.Accepted.fl_str_mv | 2014-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2014-07-31T10:29:46Z |
| datacite.date.embargoed.fl_str_mv | 2014-07-31T10:29:46Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Red blood cells deformation Microfluidic devices |
| datacite.titles.title.fl_str_mv | Visualization and measurement of red blood cells flowing in microfluidic devices |
| dc.contributor.none.fl_str_mv | Biblioteca Digital da UPB |
| dc.creator.none.fl_str_mv | Rodrigues, Raquel Oliveira Pinho, Diana Faustino, Vera Yaginuma, Tomoko Bento, David Fernandes, Carla S. Garcia, Valdemar Lima, Rui A. |
| dc.date.Accepted.fl_str_mv | 2014-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2014-07-31T10:29:46Z |
| dc.date.embargoed.fl_str_mv | 2014-07-31T10:29:46Z |
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| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/9992 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Red blood cells deformation Microfluidic devices |
| dc.title.fl_str_mv | Visualization and measurement of red blood cells flowing in microfluidic devices |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | Several experimental techniques were performed in the past years using in vitro environments, in an attempt to not only understand the blood flow behaviour in microcirculation but also develop microfluidic devices as an alternative clinical methodology to detect blood diseases. Hence, the visualization and measurement of red blood cells (RBCs) flowing in a microfluidic device are important to provide not only essential information about hydrodynamic characteristics of the blood but also vital information to diagnose the initial symptoms of diseases during clinical investigations. For instance, RBC rigidity has been correlated with myocardial infarction, diabetes mellitus, hypertension, and also other haematological disorders and diseases that affect RBC deformation more directly, such as, hereditary spherocytosis, sickle cell anaemia and malaria. Regarding a better understanding of the RBCs deformation and motion, we present in this paper a compilation of studies made in our research group, using several microfluidic devices with different microchannel geometries and fabrication techniques (i.e., soft-lithography, xurography and hybrids) that focus in the shear and extensional flow behaviour, either in healthy or chemically stiffed RBCs. |
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| person_str_mv | Rodrigues, Raquel Oliveira Pinho, Diana Pinho, Diana https://www.ciencia-id.pt/5214-ED0C-35E8 5214-ED0C-35E8 http://orcid.org/0000-0002-3884-6496 0000-0002-3884-6496 Faustino, Vera Yaginuma, Tomoko Bento, David Fernandes, Carla S. Fernandes, Carla S. https://www.ciencia-id.pt/F319-3D67-9DB7 F319-3D67-9DB7 http://orcid.org/0000-0002-3138-7493 0000-0002-3138-7493 Garcia, Valdemar Garcia, Valdemar http://orcid.org/0000-0002-3941-2438 0000-0002-3941-2438 Lima, Rui A. Lima, Rui A. https://www.ciencia-id.pt/EE12-C3FB-349D EE12-C3FB-349D http://orcid.org/0000-0003-3428-637X 0000-0003-3428-637X |
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| spelling | engporSeveral experimental techniques were performed in the past years using in vitro environments, in an attempt to not only understand the blood flow behaviour in microcirculation but also develop microfluidic devices as an alternative clinical methodology to detect blood diseases. Hence, the visualization and measurement of red blood cells (RBCs) flowing in a microfluidic device are important to provide not only essential information about hydrodynamic characteristics of the blood but also vital information to diagnose the initial symptoms of diseases during clinical investigations. For instance, RBC rigidity has been correlated with myocardial infarction, diabetes mellitus, hypertension, and also other haematological disorders and diseases that affect RBC deformation more directly, such as, hereditary spherocytosis, sickle cell anaemia and malaria. Regarding a better understanding of the RBCs deformation and motion, we present in this paper a compilation of studies made in our research group, using several microfluidic devices with different microchannel geometries and fabrication techniques (i.e., soft-lithography, xurography and hybrids) that focus in the shear and extensional flow behaviour, either in healthy or chemically stiffed RBCs.application/pdfporVisualization and measurement of red blood cells flowing in microfluidic devicesRodrigues, Raquel OliveiraPersonalPinho, DianaDSpacehttp://dspace.org/items/02bf2dd9-33ed-4d87-b61d-ca39c51f8451DSpacehttp://dspace.org/items/02bf2dd9-33ed-4d87-b61d-ca39c51f8451PinhoDianaCiência IDhttps://www.ciencia-id.pt5214-ED0C-35E8ORCIDhttp://orcid.org0000-0002-3884-6496Faustino, VeraYaginuma, TomokoBento, DavidPersonalFernandes, Carla S.DSpacehttp://dspace.org/items/8f9cf139-cfef-4025-ba87-56425618bc6eDSpacehttp://dspace.org/items/8f9cf139-cfef-4025-ba87-56425618bc6eFernandesCarla S.Ciência IDhttps://www.ciencia-id.ptF319-3D67-9DB7ORCIDhttp://orcid.org0000-0002-3138-7493Researcher IDhttps://www.researcherid.comA-4269-2015Scopus Author IDhttps://www.scopus.com8253740700PersonalGarcia, ValdemarDSpacehttp://dspace.org/items/f9422279-4412-49c2-becd-1e696a8c5c4bDSpacehttp://dspace.org/items/f9422279-4412-49c2-becd-1e696a8c5c4bGarciaValdemarORCIDhttp://orcid.org0000-0002-3941-2438PersonalLima, Rui A.DSpacehttp://dspace.org/items/7b50c499-8095-4f4f-8b1b-fa7388e4ff62DSpacehttp://dspace.org/items/7b50c499-8095-4f4f-8b1b-fa7388e4ff62LimaRui A.Ciência IDhttps://www.ciencia-id.ptEE12-C3FB-349DORCIDhttp://orcid.org0000-0003-3428-637XResearcher IDhttps://www.researcherid.comH-5157-2016Scopus Author IDhttps://www.scopus.com18437397800HostingInstitutionOrganizationalBiblioteca Digital da UPBe-mailmailto:dspace@unipb.ptdspace@unipb.pt2014-07-31T10:29:46Z20142014-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/9992http://purl.org/coar/access_right/c_abf2open accessRed blood cells deformationMicrofluidic devices223422 bytesFundação para a Ciência e a TecnologiaCSD-Chip - Desenvolvimento de um biochip para a separação e deformação de células sanguíneasinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FSAU-BEB%2F105650%2F2008/PTPTDC/SAU-BEB/105650/20085876-PPCDTICrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaANEURYSM - Hemodynamic Study in Cerebral Aneurysmsinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FEME-MFE%2F099109%2F2008/PTPTDC/EME-MFE/099109/20085876-PPCDTICrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaBIOMIMETIC - A biomimetic microdevice for the diagnosis of erythrocytes diseases based on their deformabilityinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FSAU-ENB%2F116929%2F2010/PTPTDC/SAU-ENB/116929/20105876-PPCDTICrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaMicroBloodSystems - O estudo da reologia do sangue e a migração das células sanguíneas em sistemas microfluídicosinfo:eu-repo/grantAgreement/FCT/COMPETE/EXPL%2FEMS-SIS%2F2215%2F2013/PTEXPL/EMS-SIS/2215/2013COMPETECrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaA study of the blood flow behaviour in microvascular networksinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F91192%2F2012/PTSFRH/BD/91192/2012SFRHCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaBlood Rheology and Red Blood Cell Migration in Microchannel Flowsinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F89077%2F2012/PTSFRH/BD/89077/2012SFRHCrossref Funder IDhttp://doi.org/10.13039/501100001871other research producthttp://purl.org/coar/resource_type/c_c94fconference objecthttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.unipb.pt/bitstreams/b15f29c4-73a5-427f-b47f-5145e0344112/download7th World Congress of BiomechanicsBoston |
| spellingShingle | Visualization and measurement of red blood cells flowing in microfluidic devices Rodrigues, Raquel Oliveira Red blood cells deformation Microfluidic devices |
| status | SINGLETON |
| subject.fl_str_mv | Red blood cells deformation Microfluidic devices |
| title | Visualization and measurement of red blood cells flowing in microfluidic devices |
| title_full | Visualization and measurement of red blood cells flowing in microfluidic devices |
| title_fullStr | Visualization and measurement of red blood cells flowing in microfluidic devices |
| title_full_unstemmed | Visualization and measurement of red blood cells flowing in microfluidic devices |
| title_short | Visualization and measurement of red blood cells flowing in microfluidic devices |
| title_sort | Visualization and measurement of red blood cells flowing in microfluidic devices |
| topic | Red blood cells deformation Microfluidic devices |
| topic_facet | Red blood cells deformation Microfluidic devices |
| url | http://hdl.handle.net/10198/9992 |
| visible | 1 |