Publicação
The anti-tumour effect of spearmint (Mentha spicata) in a HPV-16-transgenic mouse model
| Resumo: | Infection by Human Papillomavirus (HPV) is the main cause of cervical cancer, highlighting the importance of studying compounds that may reduce viral activity and its lesions/symptoms. The aromatic herb spearmint (Mentha spicata) (MS) has proven anti-tumor properties [1]. Thus, this study aimed to evaluate the effects of an hydroethanolic extract obtained from spearmint in HPV16-transgenic (HPV+/-) mice. The extract was obtained through maceration with ethanol/water (80:20, v/v), and the phenolic composition was determined through HPLC-DAD-ESI/MS. Thirty-three female mice (16 HPV-/- and 17 HPV+/-) were randomly divided into six groups: Group (G) I – HPV-/- w/o Mentha (n=5); G II – HPV-/- w/ Mentha (0.5 mg/ml; n=6); G III – HPV-/- w/ Mentha (0.55 mg/ml; n=5); G IV – HPV+/- w/o Mentha (n=6); G V – HPV+/- w/ Mentha (0.5 mg/ml; n=6); G VI – HPV+/- w/ Mentha (0.55 mg/ml; n=5). The spearmint extract was administered in the animal’s drinking water for 28 days. During the study, water and food intake as well the animals’ weights were recorded weekly. Afterwards, the animals were sacrificed, and their organs were collected for oxidative stress and genetic damage analysis. A total of thirteen compounds were identified in the hydroethanolic extract, being salvianolic acid B, rosmarinic acid and luteolin-7-O-glucuronide, the main compounds found. Moreover, the compounds revealed to be stable in the drinking water during the 5 tested days. Results show that HPV+/- (GIV, V and VI) have lower weight but higher water and food intake, as expected. Analysis of HPV+/- mice GIV showed a significant increase of superoxide dismutase activity when compared with GV (p=0.0029) and VI (p=0.0011). This suggests that group IV was subjected to a higher oxidative stress, as expected. These results could also mean that MS is responsible for a decrease in the oxidative stress known to be induced by HPV, which in turn decreases SOD, further the role of MS as an antioxidant. Regarding genetic damage, no statistically significant changes between groups were found in the comet and micronucleus assays, implying that spearmint has no influence on genotoxicity at the concentrations employed. There were no significant differences concerning basal DNA damage between WT and transgenic individuals as seen in other studies [2]. Further studies are required to clarify the antioxidant and antigenotoxic effects of spearmint. |
|---|---|
| Autores principais: | Jesus, T.M. |
| Outros Autores: | Silva-Reis, Rita; Pires, Maria João; Lanzarin, Germano; Félix, Luís; Venâncio, Carlos; Costa, Rui Miguel Gil; Bastos, Margarida M.S.M.; Medeiros, Rui; Nogueira, António José M.; Barros, Lillian; Ferreira, Isabel C.F.R.; Rosa, Eduardo; Oliveira, Paula A. |
| Assunto: | Spearmint (Mentha spicata) Research Subject Categories::MEDICINE::Morphology, cell biology, pathology |
| Ano: | 2021 |
| País: | Portugal |
| Tipo de documento: | póster em conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Politécnica de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital da UPB |
| _version_ | 1877782911327928320 |
|---|---|
| author | Jesus, T.M. |
| author2 | Silva-Reis, Rita Pires, Maria João Lanzarin, Germano Félix, Luís Venâncio, Carlos Costa, Rui Miguel Gil Bastos, Margarida M.S.M. Medeiros, Rui Nogueira, António José M. Barros, Lillian Ferreira, Isabel C.F.R. Rosa, Eduardo Oliveira, Paula A. |
| author2_role | author author author author author author author author author author author author author |
| author_facet | Jesus, T.M. Silva-Reis, Rita Pires, Maria João Lanzarin, Germano Félix, Luís Venâncio, Carlos Costa, Rui Miguel Gil Bastos, Margarida M.S.M. Medeiros, Rui Nogueira, António José M. Barros, Lillian Ferreira, Isabel C.F.R. Rosa, Eduardo Oliveira, Paula A. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital da UPB |
| country_str | PT |
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| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital da UPB |
| datacite.creators.creator.creatorName.fl_str_mv | Jesus, T.M. Silva-Reis, Rita Pires, Maria João Lanzarin, Germano Félix, Luís Venâncio, Carlos Costa, Rui Miguel Gil Bastos, Margarida M.S.M. Medeiros, Rui Nogueira, António José M. Barros, Lillian Ferreira, Isabel C.F.R. Rosa, Eduardo Oliveira, Paula A. |
| datacite.date.Accepted.fl_str_mv | 2021-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2023-03-15T11:23:15Z |
| datacite.date.embargoed.fl_str_mv | 2023-03-15T11:23:15Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Spearmint (Mentha spicata) Research Subject Categories::MEDICINE::Morphology, cell biology, pathology |
| datacite.titles.title.fl_str_mv | The anti-tumour effect of spearmint (Mentha spicata) in a HPV-16-transgenic mouse model |
| dc.contributor.none.fl_str_mv | Biblioteca Digital da UPB |
| dc.creator.none.fl_str_mv | Jesus, T.M. Silva-Reis, Rita Pires, Maria João Lanzarin, Germano Félix, Luís Venâncio, Carlos Costa, Rui Miguel Gil Bastos, Margarida M.S.M. Medeiros, Rui Nogueira, António José M. Barros, Lillian Ferreira, Isabel C.F.R. Rosa, Eduardo Oliveira, Paula A. |
| dc.date.Accepted.fl_str_mv | 2021-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2023-03-15T11:23:15Z |
| dc.date.embargoed.fl_str_mv | 2023-03-15T11:23:15Z |
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| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/27736 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Instituto Politécnico de Bragança |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Spearmint (Mentha spicata) Research Subject Categories::MEDICINE::Morphology, cell biology, pathology |
| dc.title.fl_str_mv | The anti-tumour effect of spearmint (Mentha spicata) in a HPV-16-transgenic mouse model |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6670 |
| description | Infection by Human Papillomavirus (HPV) is the main cause of cervical cancer, highlighting the importance of studying compounds that may reduce viral activity and its lesions/symptoms. The aromatic herb spearmint (Mentha spicata) (MS) has proven anti-tumor properties [1]. Thus, this study aimed to evaluate the effects of an hydroethanolic extract obtained from spearmint in HPV16-transgenic (HPV+/-) mice. The extract was obtained through maceration with ethanol/water (80:20, v/v), and the phenolic composition was determined through HPLC-DAD-ESI/MS. Thirty-three female mice (16 HPV-/- and 17 HPV+/-) were randomly divided into six groups: Group (G) I – HPV-/- w/o Mentha (n=5); G II – HPV-/- w/ Mentha (0.5 mg/ml; n=6); G III – HPV-/- w/ Mentha (0.55 mg/ml; n=5); G IV – HPV+/- w/o Mentha (n=6); G V – HPV+/- w/ Mentha (0.5 mg/ml; n=6); G VI – HPV+/- w/ Mentha (0.55 mg/ml; n=5). The spearmint extract was administered in the animal’s drinking water for 28 days. During the study, water and food intake as well the animals’ weights were recorded weekly. Afterwards, the animals were sacrificed, and their organs were collected for oxidative stress and genetic damage analysis. A total of thirteen compounds were identified in the hydroethanolic extract, being salvianolic acid B, rosmarinic acid and luteolin-7-O-glucuronide, the main compounds found. Moreover, the compounds revealed to be stable in the drinking water during the 5 tested days. Results show that HPV+/- (GIV, V and VI) have lower weight but higher water and food intake, as expected. Analysis of HPV+/- mice GIV showed a significant increase of superoxide dismutase activity when compared with GV (p=0.0029) and VI (p=0.0011). This suggests that group IV was subjected to a higher oxidative stress, as expected. These results could also mean that MS is responsible for a decrease in the oxidative stress known to be induced by HPV, which in turn decreases SOD, further the role of MS as an antioxidant. Regarding genetic damage, no statistically significant changes between groups were found in the comet and micronucleus assays, implying that spearmint has no influence on genotoxicity at the concentrations employed. There were no significant differences concerning basal DNA damage between WT and transgenic individuals as seen in other studies [2]. Further studies are required to clarify the antioxidant and antigenotoxic effects of spearmint. |
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| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia |
| funding.identifier_str_mv | UIDB/00690/2020 |
| funding.name_str_mv | 6817 - DCRRNI ID |
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| person_str_mv | Jesus, T.M. Silva-Reis, Rita Pires, Maria João Lanzarin, Germano Félix, Luís Venâncio, Carlos Costa, Rui Miguel Gil Bastos, Margarida M.S.M. Medeiros, Rui Nogueira, António José M. Nogueira, António José M. https://www.ciencia-id.pt/8A11-794E-36BD 8A11-794E-36BD http://orcid.org/0000-0001-8912-7355 0000-0001-8912-7355 Barros, Lillian Barros, Lillian https://www.ciencia-id.pt/9616-35CB-D001 9616-35CB-D001 http://orcid.org/0000-0002-9050-5189 0000-0002-9050-5189 Ferreira, Isabel C.F.R. Ferreira, Isabel C.F.R. https://www.ciencia-id.pt/9418-CF95-9919 9418-CF95-9919 http://orcid.org/0000-0003-4910-4882 0000-0003-4910-4882 Rosa, Eduardo Oliveira, Paula A. |
| publishDate | 2021 |
| publisher.none.fl_str_mv | Instituto Politécnico de Bragança |
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| spelling | engInstituto Politécnico de Bragançapt_PTInfection by Human Papillomavirus (HPV) is the main cause of cervical cancer, highlighting the importance of studying compounds that may reduce viral activity and its lesions/symptoms. The aromatic herb spearmint (Mentha spicata) (MS) has proven anti-tumor properties [1]. Thus, this study aimed to evaluate the effects of an hydroethanolic extract obtained from spearmint in HPV16-transgenic (HPV+/-) mice. The extract was obtained through maceration with ethanol/water (80:20, v/v), and the phenolic composition was determined through HPLC-DAD-ESI/MS. Thirty-three female mice (16 HPV-/- and 17 HPV+/-) were randomly divided into six groups: Group (G) I – HPV-/- w/o Mentha (n=5); G II – HPV-/- w/ Mentha (0.5 mg/ml; n=6); G III – HPV-/- w/ Mentha (0.55 mg/ml; n=5); G IV – HPV+/- w/o Mentha (n=6); G V – HPV+/- w/ Mentha (0.5 mg/ml; n=6); G VI – HPV+/- w/ Mentha (0.55 mg/ml; n=5). The spearmint extract was administered in the animal’s drinking water for 28 days. During the study, water and food intake as well the animals’ weights were recorded weekly. Afterwards, the animals were sacrificed, and their organs were collected for oxidative stress and genetic damage analysis. A total of thirteen compounds were identified in the hydroethanolic extract, being salvianolic acid B, rosmarinic acid and luteolin-7-O-glucuronide, the main compounds found. Moreover, the compounds revealed to be stable in the drinking water during the 5 tested days. Results show that HPV+/- (GIV, V and VI) have lower weight but higher water and food intake, as expected. Analysis of HPV+/- mice GIV showed a significant increase of superoxide dismutase activity when compared with GV (p=0.0029) and VI (p=0.0011). This suggests that group IV was subjected to a higher oxidative stress, as expected. These results could also mean that MS is responsible for a decrease in the oxidative stress known to be induced by HPV, which in turn decreases SOD, further the role of MS as an antioxidant. Regarding genetic damage, no statistically significant changes between groups were found in the comet and micronucleus assays, implying that spearmint has no influence on genotoxicity at the concentrations employed. There were no significant differences concerning basal DNA damage between WT and transgenic individuals as seen in other studies [2]. Further studies are required to clarify the antioxidant and antigenotoxic effects of spearmint.application/pdfpt_PTThe anti-tumour effect of spearmint (Mentha spicata) in a HPV-16-transgenic mouse modelJesus, T.M.Silva-Reis, RitaPires, Maria JoãoLanzarin, GermanoFélix, LuísVenâncio, CarlosCosta, Rui Miguel GilBastos, Margarida M.S.M.Medeiros, RuiPersonalNogueira, António José M.DSpacehttp://dspace.org/items/648209a2-eec9-4bec-975a-32673e41b95cDSpacehttp://dspace.org/items/648209a2-eec9-4bec-975a-32673e41b95cNogueiraAntónio José M.Ciência IDhttps://www.ciencia-id.pt8A11-794E-36BDORCIDhttp://orcid.org0000-0001-8912-7355Scopus Author IDhttps://www.scopus.com55865568268PersonalBarros, LillianDSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01DSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01BarrosLillianCiência IDhttps://www.ciencia-id.pt9616-35CB-D001ORCIDhttp://orcid.org0000-0002-9050-5189Researcher IDhttps://www.researcherid.comJ-3600-2013Researcher IDhttps://www.researcherid.comJ-3600-2013Scopus Author IDhttps://www.scopus.com35236343600Scopus Author IDhttps://www.scopus.com57194900801PersonalFerreira, Isabel C.F.R.DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8FerreiraIsabel C.F.R.Ciência IDhttps://www.ciencia-id.pt9418-CF95-9919ORCIDhttp://orcid.org0000-0003-4910-4882Researcher IDhttps://www.researcherid.comE-8500-2013Scopus Author IDhttps://www.scopus.com36868826600Rosa, EduardoOliveira, Paula A.HostingInstitutionOrganizationalBiblioteca Digital da UPBe-mailmailto:dspace@unipb.ptdspace@unipb.ptISBNIsPartOf978-972-745-286-62023-03-15T11:23:15Z20212021-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/27736http://purl.org/coar/access_right/c_abf2open accessSpearmint (Mentha spicata)Research Subject Categories::MEDICINE::Morphology, cell biology, pathology553252 bytesFundação para a Ciência e a TecnologiaMountain Research Center - UIDB/00690/2020info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PTUIDB/00690/20206817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871other research producthttp://purl.org/coar/resource_type/c_6670conference poster2021http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.unipb.pt/bitstreams/429392fc-8a6e-406f-877a-aaa2b27e41e6/download1st Natural products application: Health, Cosmetic and FoodBragança |
| spellingShingle | The anti-tumour effect of spearmint (Mentha spicata) in a HPV-16-transgenic mouse model Jesus, T.M. Spearmint (Mentha spicata) Research Subject Categories::MEDICINE::Morphology, cell biology, pathology |
| status | SINGLETON |
| subject.fl_str_mv | Spearmint (Mentha spicata) Research Subject Categories::MEDICINE::Morphology, cell biology, pathology |
| title | The anti-tumour effect of spearmint (Mentha spicata) in a HPV-16-transgenic mouse model |
| title_full | The anti-tumour effect of spearmint (Mentha spicata) in a HPV-16-transgenic mouse model |
| title_fullStr | The anti-tumour effect of spearmint (Mentha spicata) in a HPV-16-transgenic mouse model |
| title_full_unstemmed | The anti-tumour effect of spearmint (Mentha spicata) in a HPV-16-transgenic mouse model |
| title_short | The anti-tumour effect of spearmint (Mentha spicata) in a HPV-16-transgenic mouse model |
| title_sort | The anti-tumour effect of spearmint (Mentha spicata) in a HPV-16-transgenic mouse model |
| topic | Spearmint (Mentha spicata) Research Subject Categories::MEDICINE::Morphology, cell biology, pathology |
| topic_facet | Spearmint (Mentha spicata) Research Subject Categories::MEDICINE::Morphology, cell biology, pathology |
| url | http://hdl.handle.net/10198/27736 |
| visible | 1 |