Publicação
Cell-free layer measurements of in vitro blood flow in a microfluidic network: an automatic and manual approach
| Resumo: | In microcirculation, the cell-free layer (CFL) is a well-known physiological phenomenon that plays an important role in reducing the flow resistance and in balancing nitric oxide (NO) production by endothelial cells and NO scavenging by red blood cells. To better understand this phenomenon, several blood flow studies have been performed in simple geometries at both in vivo and in vitro environments. However, to date little information is available regarding the effects imposed by a complex branching network on the CFL. The present study shows the CFL layer variation at a microchannel network. The images were captured using a high-speed video microscopy system and the thickness of the CFL was measured using both manual and automatic image analysis techniques. Using this methodology, it was possible to visualise the in vitro blood flowing through the network and to identify several flow phenomena that happen in microcirculation. Overall, the results have shown that the concentration of cells and the geometrical configuration of the network have a major impact on the CFL thickness. In particular, the thickness of the CFL decreases as the fluid flows through a microchannel network composed with successive smaller channels. It was also clear that, for the full length of the network, the CFL thickness tends to decrease with the increase of the concentration of cells. The automatic method developed becomes inaccurate for high haematocrit and needs be calibrated by manual methods for Hcts bigger than 10%. The results obtained from this study could help the development and validation of multiscale numerical models able to take into account the CFL for simulating microvascular blood flow. |
|---|---|
| Autores principais: | Bento, David |
| Outros Autores: | Pereira, Ana I.; Lima, José; Miranda, João Mário; Lima, Rui A. |
| Assunto: | Cell-free layer Microfluidic network Red blood cells |
| Ano: | 2017 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Politécnica de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital da UPB |
| _version_ | 1877782637988282368 |
|---|---|
| author | Bento, David |
| author2 | Pereira, Ana I. Lima, José Miranda, João Mário Lima, Rui A. |
| author2_role | author author author author |
| author_facet | Bento, David Pereira, Ana I. Lima, José Miranda, João Mário Lima, Rui A. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital da UPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Bento, David\"},{\"Person.name\":\"Pereira, Ana I.\",\"Person.identifier.cienciaID\":\"0716-B7C2-93E4\",\"Person.identifier.orcid\":\"0000-0003-3803-2043\",\"Person.identifier.researcherID\":\"F-3168-2010\",\"Person.identifier.scopusAuthorID\":\"15071961600\"},{\"Person.name\":\"Lima, José\",\"Person.identifier.cienciaID\":\"6016-C902-86A9\",\"Person.identifier.orcid\":\"0000-0001-7902-1207\",\"Person.identifier.researcherID\":\"L-3370-2014\",\"Person.identifier.scopusAuthorID\":\"55851941311\"},{\"Person.name\":\"Miranda, João Mário\"},{\"Person.name\":\"Lima, Rui A.\",\"Person.identifier.cienciaID\":\"EE12-C3FB-349D\",\"Person.identifier.orcid\":\"0000-0003-3428-637X\",\"Person.identifier.researcherID\":\"H-5157-2016\",\"Person.identifier.scopusAuthorID\":\"18437397800\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital da UPB |
| datacite.creators.creator.creatorName.fl_str_mv | Bento, David Pereira, Ana I. Lima, José Miranda, João Mário Lima, Rui A. |
| datacite.date.Accepted.fl_str_mv | 2017-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-04-24T10:48:38Z |
| datacite.date.embargoed.fl_str_mv | 2018-04-24T10:48:38Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Cell-free layer Microfluidic network Red blood cells |
| datacite.titles.title.fl_str_mv | Cell-free layer measurements of in vitro blood flow in a microfluidic network: an automatic and manual approach |
| dc.contributor.none.fl_str_mv | Biblioteca Digital da UPB |
| dc.creator.none.fl_str_mv | Bento, David Pereira, Ana I. Lima, José Miranda, João Mário Lima, Rui A. |
| dc.date.Accepted.fl_str_mv | 2017-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-04-24T10:48:38Z |
| dc.date.embargoed.fl_str_mv | 2018-04-24T10:48:38Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/17228 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Cell-free layer Microfluidic network Red blood cells |
| dc.title.fl_str_mv | Cell-free layer measurements of in vitro blood flow in a microfluidic network: an automatic and manual approach |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | In microcirculation, the cell-free layer (CFL) is a well-known physiological phenomenon that plays an important role in reducing the flow resistance and in balancing nitric oxide (NO) production by endothelial cells and NO scavenging by red blood cells. To better understand this phenomenon, several blood flow studies have been performed in simple geometries at both in vivo and in vitro environments. However, to date little information is available regarding the effects imposed by a complex branching network on the CFL. The present study shows the CFL layer variation at a microchannel network. The images were captured using a high-speed video microscopy system and the thickness of the CFL was measured using both manual and automatic image analysis techniques. Using this methodology, it was possible to visualise the in vitro blood flowing through the network and to identify several flow phenomena that happen in microcirculation. Overall, the results have shown that the concentration of cells and the geometrical configuration of the network have a major impact on the CFL thickness. In particular, the thickness of the CFL decreases as the fluid flows through a microchannel network composed with successive smaller channels. It was also clear that, for the full length of the network, the CFL thickness tends to decrease with the increase of the concentration of cells. The automatic method developed becomes inaccurate for high haematocrit and needs be calibrated by manual methods for Hcts bigger than 10%. The results obtained from this study could help the development and validation of multiscale numerical models able to take into account the CFL for simulating microvascular blood flow. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.unipb.pt/bitstreams/1c83d019-edd5-4545-ac31-cadfa0a8380f/download |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10198/17228 |
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| institution | Universidade Politécnica de Bragança |
| instname_str | Universidade Politécnica de Bragança |
| language | eng |
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| network_name_str | Biblioteca Digital da UPB |
| oai_identifier_str | oai:bibliotecadigital.unipb.pt:10198/17228 |
| organization_str_mv | urn:organizationAcronym:unipb |
| person_str_mv | Bento, David Pereira, Ana I. Pereira, Ana I. https://www.ciencia-id.pt/0716-B7C2-93E4 0716-B7C2-93E4 http://orcid.org/0000-0003-3803-2043 0000-0003-3803-2043 Lima, José Lima, José https://www.ciencia-id.pt/6016-C902-86A9 6016-C902-86A9 http://orcid.org/0000-0001-7902-1207 0000-0001-7902-1207 Miranda, João Mário Lima, Rui A. Lima, Rui A. https://www.ciencia-id.pt/EE12-C3FB-349D EE12-C3FB-349D http://orcid.org/0000-0003-3428-637X 0000-0003-3428-637X |
| publishDate | 2017 |
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| reponame_str | Biblioteca Digital da UPB |
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| spelling | engpt_PTIn microcirculation, the cell-free layer (CFL) is a well-known physiological phenomenon that plays an important role in reducing the flow resistance and in balancing nitric oxide (NO) production by endothelial cells and NO scavenging by red blood cells. To better understand this phenomenon, several blood flow studies have been performed in simple geometries at both in vivo and in vitro environments. However, to date little information is available regarding the effects imposed by a complex branching network on the CFL. The present study shows the CFL layer variation at a microchannel network. The images were captured using a high-speed video microscopy system and the thickness of the CFL was measured using both manual and automatic image analysis techniques. Using this methodology, it was possible to visualise the in vitro blood flowing through the network and to identify several flow phenomena that happen in microcirculation. Overall, the results have shown that the concentration of cells and the geometrical configuration of the network have a major impact on the CFL thickness. In particular, the thickness of the CFL decreases as the fluid flows through a microchannel network composed with successive smaller channels. It was also clear that, for the full length of the network, the CFL thickness tends to decrease with the increase of the concentration of cells. The automatic method developed becomes inaccurate for high haematocrit and needs be calibrated by manual methods for Hcts bigger than 10%. The results obtained from this study could help the development and validation of multiscale numerical models able to take into account the CFL for simulating microvascular blood flow.application/pdfpt_PTCell-free layer measurements of in vitro blood flow in a microfluidic network: an automatic and manual approachBento, DavidPersonalPereira, Ana I.DSpacehttp://dspace.org/items/e9981d62-2a2b-4fef-b75e-c2a14b0e7846DSpacehttp://dspace.org/items/e9981d62-2a2b-4fef-b75e-c2a14b0e7846PereiraAna I.Ciência IDhttps://www.ciencia-id.pt0716-B7C2-93E4ORCIDhttp://orcid.org0000-0003-3803-2043Researcher IDhttps://www.researcherid.comF-3168-2010Scopus Author IDhttps://www.scopus.com15071961600PersonalLima, JoséDSpacehttp://dspace.org/items/d88c2b2a-efc2-48ef-b1fd-1145475e0055DSpacehttp://dspace.org/items/d88c2b2a-efc2-48ef-b1fd-1145475e0055LimaJoséCiência IDhttps://www.ciencia-id.pt6016-C902-86A9ORCIDhttp://orcid.org0000-0001-7902-1207Researcher IDhttps://www.researcherid.comL-3370-2014Scopus Author IDhttps://www.scopus.com55851941311Miranda, João MárioPersonalLima, Rui A.DSpacehttp://dspace.org/items/7b50c499-8095-4f4f-8b1b-fa7388e4ff62DSpacehttp://dspace.org/items/7b50c499-8095-4f4f-8b1b-fa7388e4ff62LimaRui A.Ciência IDhttps://www.ciencia-id.ptEE12-C3FB-349DORCIDhttp://orcid.org0000-0003-3428-637XResearcher IDhttps://www.researcherid.comH-5157-2016Scopus Author IDhttps://www.scopus.com18437397800HostingInstitutionOrganizationalBiblioteca Digital da UPBe-mailmailto:dspace@unipb.ptdspace@unipb.ptDOIIsPartOf10.1080/21681163.2017.13290292018-04-24T10:48:38Z20172017-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/17228http://purl.org/coar/access_right/c_abf2open accessCell-free layerMicrofluidic networkRed blood cells2029870 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2017http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.unipb.pt/bitstreams/1c83d019-edd5-4545-ac31-cadfa0a8380f/downloadComputer Methods in Biomechanics and Biomedical Engineering: Imaging & Visualization19 |
| spellingShingle | Cell-free layer measurements of in vitro blood flow in a microfluidic network: an automatic and manual approach Bento, David Cell-free layer Microfluidic network Red blood cells |
| status | SINGLETON |
| subject.fl_str_mv | Cell-free layer Microfluidic network Red blood cells |
| title | Cell-free layer measurements of in vitro blood flow in a microfluidic network: an automatic and manual approach |
| title_full | Cell-free layer measurements of in vitro blood flow in a microfluidic network: an automatic and manual approach |
| title_fullStr | Cell-free layer measurements of in vitro blood flow in a microfluidic network: an automatic and manual approach |
| title_full_unstemmed | Cell-free layer measurements of in vitro blood flow in a microfluidic network: an automatic and manual approach |
| title_short | Cell-free layer measurements of in vitro blood flow in a microfluidic network: an automatic and manual approach |
| title_sort | Cell-free layer measurements of in vitro blood flow in a microfluidic network: an automatic and manual approach |
| topic | Cell-free layer Microfluidic network Red blood cells |
| topic_facet | Cell-free layer Microfluidic network Red blood cells |
| url | http://hdl.handle.net/10198/17228 |
| visible | 1 |
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