Publicação
Maximization of fructose esters synthesis by response surface methodology
| Resumo: | Enzymatic synthesis of fructose fatty acid ester was performed in organic solvent media, using a purified lipase from Candida antartica B immobilized in acrylic resin. Response surface methodology with a central composite rotatable design based on five levels was implemented to optimize three experimental operating conditions (temperature, agitation and reaction time). A statistical significant cubic model was established. Temperature and reaction time were found to be the most significant parameters. The optimum operational conditions for maximizing the synthesis of fructose esters were 57.18C, 100 rpm and 37.8 h. The model was validated in the identified optimal conditions to check its adequacy and accuracy, and an experimental esterification percentage of 88.4% (+/-0.3%) was obtained. These results showed that an improvement of the enzymatic synthesis of fructose esters was obtained under the optimized conditions. |
|---|---|
| Autores principais: | Neta, Nair S. |
| Outros Autores: | Peres, António M.; Teixeira, José; Rodrigues, Lígia R. |
| Assunto: | Candida antartica B Fructose esters Response surface methodology |
| Ano: | 2011 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade Politécnica de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital da UPB |
| _version_ | 1877782360551849984 |
|---|---|
| author | Neta, Nair S. |
| author2 | Peres, António M. Teixeira, José Rodrigues, Lígia R. |
| author2_role | author author author |
| author_facet | Neta, Nair S. Peres, António M. Teixeira, José Rodrigues, Lígia R. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital da UPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Neta, Nair S.\"},{\"Person.name\":\"Peres, António M.\",\"Person.identifier.cienciaID\":\"CF16-5443-F420\",\"Person.identifier.orcid\":\"0000-0001-6595-9165\",\"Person.identifier.researcherID\":\"I-8470-2012\",\"Person.identifier.scopusAuthorID\":\"7102331969\"},{\"Person.name\":\"Teixeira, José\"},{\"Person.name\":\"Rodrigues, Lígia R.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital da UPB |
| datacite.creators.creator.creatorName.fl_str_mv | Neta, Nair S. Peres, António M. Teixeira, José Rodrigues, Lígia R. |
| datacite.date.Accepted.fl_str_mv | 2011-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2011-12-06T14:14:32Z |
| datacite.date.embargoed.fl_str_mv | 2011-12-06T14:14:32Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Candida antartica B Fructose esters Response surface methodology |
| datacite.titles.title.fl_str_mv | Maximization of fructose esters synthesis by response surface methodology |
| dc.contributor.none.fl_str_mv | Biblioteca Digital da UPB |
| dc.creator.none.fl_str_mv | Neta, Nair S. Peres, António M. Teixeira, José Rodrigues, Lígia R. |
| dc.date.Accepted.fl_str_mv | 2011-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2011-12-06T14:14:32Z |
| dc.date.embargoed.fl_str_mv | 2011-12-06T14:14:32Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/6406 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Candida antartica B Fructose esters Response surface methodology |
| dc.title.fl_str_mv | Maximization of fructose esters synthesis by response surface methodology |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Enzymatic synthesis of fructose fatty acid ester was performed in organic solvent media, using a purified lipase from Candida antartica B immobilized in acrylic resin. Response surface methodology with a central composite rotatable design based on five levels was implemented to optimize three experimental operating conditions (temperature, agitation and reaction time). A statistical significant cubic model was established. Temperature and reaction time were found to be the most significant parameters. The optimum operational conditions for maximizing the synthesis of fructose esters were 57.18C, 100 rpm and 37.8 h. The model was validated in the identified optimal conditions to check its adequacy and accuracy, and an experimental esterification percentage of 88.4% (+/-0.3%) was obtained. These results showed that an improvement of the enzymatic synthesis of fructose esters was obtained under the optimized conditions. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.unipb.pt/bitstreams/3b633a23-b142-4e2e-87d5-285f1a18e652/download |
| id | ipb_2f7ccee9bebdaf049cae796a6388fa40 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/6406 |
| inst_facet_str | urn:organizationAcronym:unipb{{{_:::_}}}Universidade Politécnica de Bragança |
| instacron_str | unipb |
| institution | Universidade Politécnica de Bragança |
| instname_str | Universidade Politécnica de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital da UPB |
| oai_identifier_str | oai:bibliotecadigital.unipb.pt:10198/6406 |
| organization_str_mv | urn:organizationAcronym:unipb |
| person_str_mv | Neta, Nair S. Peres, António M. Peres, António M. https://www.ciencia-id.pt/CF16-5443-F420 CF16-5443-F420 http://orcid.org/0000-0001-6595-9165 0000-0001-6595-9165 Teixeira, José Rodrigues, Lígia R. |
| publishDate | 2011 |
| publisher.none.fl_str_mv | Elsevier |
| repo_facet_str | urn:repositoryAcronym:ipb{{{_:::_}}}Biblioteca Digital da UPB |
| reponame_str | Biblioteca Digital da UPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engElsevierporEnzymatic synthesis of fructose fatty acid ester was performed in organic solvent media, using a purified lipase from Candida antartica B immobilized in acrylic resin. Response surface methodology with a central composite rotatable design based on five levels was implemented to optimize three experimental operating conditions (temperature, agitation and reaction time). A statistical significant cubic model was established. Temperature and reaction time were found to be the most significant parameters. The optimum operational conditions for maximizing the synthesis of fructose esters were 57.18C, 100 rpm and 37.8 h. The model was validated in the identified optimal conditions to check its adequacy and accuracy, and an experimental esterification percentage of 88.4% (+/-0.3%) was obtained. These results showed that an improvement of the enzymatic synthesis of fructose esters was obtained under the optimized conditions.application/pdfporMaximization of fructose esters synthesis by response surface methodologyNeta, Nair S.PersonalPeres, António M.DSpacehttp://dspace.org/items/7d93be47-8dc4-4413-9304-5b978773d3bbDSpacehttp://dspace.org/items/7d93be47-8dc4-4413-9304-5b978773d3bbPeresAntónio M.Ciência IDhttps://www.ciencia-id.ptCF16-5443-F420ORCIDhttp://orcid.org0000-0001-6595-9165Researcher IDhttps://www.researcherid.comI-8470-2012Scopus Author IDhttps://www.scopus.com7102331969Teixeira, JoséRodrigues, Lígia R.HostingInstitutionOrganizationalBiblioteca Digital da UPBe-mailmailto:dspace@unipb.ptdspace@unipb.ptISSNIsPartOf1871-6784DOIIsPartOf10.1016/j.nbt.2011.02.0072011-12-06T14:14:32Z20112011-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/6406http://purl.org/coar/access_right/c_16ecrestricted accessCandida antartica BFructose estersResponse surface methodology349163 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://bibliotecadigital.unipb.pt/bitstreams/3b633a23-b142-4e2e-87d5-285f1a18e652/downloadNew Biotechnology28349355 |
| spellingShingle | Maximization of fructose esters synthesis by response surface methodology Neta, Nair S. Candida antartica B Fructose esters Response surface methodology |
| status | SINGLETON |
| subject.fl_str_mv | Candida antartica B Fructose esters Response surface methodology |
| title | Maximization of fructose esters synthesis by response surface methodology |
| title_full | Maximization of fructose esters synthesis by response surface methodology |
| title_fullStr | Maximization of fructose esters synthesis by response surface methodology |
| title_full_unstemmed | Maximization of fructose esters synthesis by response surface methodology |
| title_short | Maximization of fructose esters synthesis by response surface methodology |
| title_sort | Maximization of fructose esters synthesis by response surface methodology |
| topic | Candida antartica B Fructose esters Response surface methodology |
| topic_facet | Candida antartica B Fructose esters Response surface methodology |
| url | http://hdl.handle.net/10198/6406 |
| visible | 1 |
Atividades financiadas
Não foram encontrados projetos financiados.