Publicação
Transport of acetic acid in Zygosaccharomyces bailii: effects of ethanol and implications on the resistance of the yeast to acid environments
| Resumo: | Cells of Zygosaccharomyces bailii ISA 1307 grown in a medium with acetic acid, ethanol, or glycerol as the sole carbon and energy source transported acetic acid by a saturable transport system. This system accepted propionic and formic acids but not lactic, sorbic, and benzoic acids. When the carbon source was glucose or fructose, the cells displayed activity of a mediated transport system specific for acetic acid, apparently not being able to recognize other monocarboxylic acids. In both types of cells, ethanol inhibited the transport of labelled acetic acid. The inhibition was noncompetitive, and the dependence of the maximum transport rate on the ethanol concentration was found to be exponential. These results reinforced the belief that, under the referenced growth conditions, the acid entered the cells mainly through a transporter protein. The simple diffusion of the undissociated acid appeared to contribute, with a relatively low weight, to the overall acid uptake. It was concluded that in Z. bailii, ethanol plays a protective role against the possible negative effects of acetic acid by inhibiting its transport and accumulation. Thus, the intracellular concentration of the acid could be maintained at levels lower than those expected if the acid entered the cells only by simple diffusion. |
|---|---|
| Autores principais: | Sousa, Maria João |
| Outros Autores: | Estevinho, Leticia M.; Côrte-Real, Manuela; Leão, Cecília |
| Assunto: | Zygosaccharomyces bailii |
| Ano: | 1996 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Politécnica de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital da UPB |
| _version_ | 1877782273832517632 |
|---|---|
| author | Sousa, Maria João |
| author2 | Estevinho, Leticia M. Côrte-Real, Manuela Leão, Cecília |
| author2_role | author author author |
| author_facet | Sousa, Maria João Estevinho, Leticia M. Côrte-Real, Manuela Leão, Cecília |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital da UPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Sousa, Maria João\",\"Person.identifier.cienciaID\":\"D91E-3571-3979\",\"Person.identifier.orcid\":\"0000-0002-9946-4926\",\"Person.identifier.scopusAuthorID\":\"55230282700\"},{\"Person.name\":\"Estevinho, Leticia M.\",\"Person.identifier.cienciaID\":\"BA14-09D6-A406\",\"Person.identifier.orcid\":\"0000-0002-9249-1948\",\"Person.identifier.scopusAuthorID\":\"6506577664\",\"Person.identifier.scopusAuthorID\":\"57211945650\"},{\"Person.name\":\"Côrte-Real, Manuela\"},{\"Person.name\":\"Leão, Cecília\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital da UPB |
| datacite.creators.creator.creatorName.fl_str_mv | Sousa, Maria João Estevinho, Leticia M. Côrte-Real, Manuela Leão, Cecília |
| datacite.date.Accepted.fl_str_mv | 1996-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2011-05-19T09:42:56Z |
| datacite.date.embargoed.fl_str_mv | 2011-05-19T09:42:56Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Zygosaccharomyces bailii |
| datacite.titles.title.fl_str_mv | Transport of acetic acid in Zygosaccharomyces bailii: effects of ethanol and implications on the resistance of the yeast to acid environments |
| dc.contributor.none.fl_str_mv | Biblioteca Digital da UPB |
| dc.creator.none.fl_str_mv | Sousa, Maria João Estevinho, Leticia M. Côrte-Real, Manuela Leão, Cecília |
| dc.date.Accepted.fl_str_mv | 1996-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2011-05-19T09:42:56Z |
| dc.date.embargoed.fl_str_mv | 2011-05-19T09:42:56Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/4395 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | American Society of Microbiology |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Zygosaccharomyces bailii |
| dc.title.fl_str_mv | Transport of acetic acid in Zygosaccharomyces bailii: effects of ethanol and implications on the resistance of the yeast to acid environments |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Cells of Zygosaccharomyces bailii ISA 1307 grown in a medium with acetic acid, ethanol, or glycerol as the sole carbon and energy source transported acetic acid by a saturable transport system. This system accepted propionic and formic acids but not lactic, sorbic, and benzoic acids. When the carbon source was glucose or fructose, the cells displayed activity of a mediated transport system specific for acetic acid, apparently not being able to recognize other monocarboxylic acids. In both types of cells, ethanol inhibited the transport of labelled acetic acid. The inhibition was noncompetitive, and the dependence of the maximum transport rate on the ethanol concentration was found to be exponential. These results reinforced the belief that, under the referenced growth conditions, the acid entered the cells mainly through a transporter protein. The simple diffusion of the undissociated acid appeared to contribute, with a relatively low weight, to the overall acid uptake. It was concluded that in Z. bailii, ethanol plays a protective role against the possible negative effects of acetic acid by inhibiting its transport and accumulation. Thus, the intracellular concentration of the acid could be maintained at levels lower than those expected if the acid entered the cells only by simple diffusion. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.unipb.pt/bitstreams/5382b11a-1a90-40d4-ad20-9009f11097fa/download |
| id | ipb_13f46fc26387c8a2dca79bc4de6d0dec |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/4395 |
| inst_facet_str | urn:organizationAcronym:unipb{{{_:::_}}}Universidade Politécnica de Bragança |
| instacron_str | unipb |
| institution | Universidade Politécnica de Bragança |
| instname_str | Universidade Politécnica de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital da UPB |
| oai_identifier_str | oai:bibliotecadigital.unipb.pt:10198/4395 |
| organization_str_mv | urn:organizationAcronym:unipb |
| person_str_mv | Sousa, Maria João Sousa, Maria João https://www.ciencia-id.pt/D91E-3571-3979 D91E-3571-3979 http://orcid.org/0000-0002-9946-4926 0000-0002-9946-4926 Estevinho, Leticia M. Estevinho, Leticia M. https://www.ciencia-id.pt/BA14-09D6-A406 BA14-09D6-A406 http://orcid.org/0000-0002-9249-1948 0000-0002-9249-1948 Côrte-Real, Manuela Leão, Cecília |
| publishDate | 1996 |
| publisher.none.fl_str_mv | American Society of Microbiology |
| repo_facet_str | urn:repositoryAcronym:ipb{{{_:::_}}}Biblioteca Digital da UPB |
| reponame_str | Biblioteca Digital da UPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engAmerican Society of MicrobiologyporCells of Zygosaccharomyces bailii ISA 1307 grown in a medium with acetic acid, ethanol, or glycerol as the sole carbon and energy source transported acetic acid by a saturable transport system. This system accepted propionic and formic acids but not lactic, sorbic, and benzoic acids. When the carbon source was glucose or fructose, the cells displayed activity of a mediated transport system specific for acetic acid, apparently not being able to recognize other monocarboxylic acids. In both types of cells, ethanol inhibited the transport of labelled acetic acid. The inhibition was noncompetitive, and the dependence of the maximum transport rate on the ethanol concentration was found to be exponential. These results reinforced the belief that, under the referenced growth conditions, the acid entered the cells mainly through a transporter protein. The simple diffusion of the undissociated acid appeared to contribute, with a relatively low weight, to the overall acid uptake. It was concluded that in Z. bailii, ethanol plays a protective role against the possible negative effects of acetic acid by inhibiting its transport and accumulation. Thus, the intracellular concentration of the acid could be maintained at levels lower than those expected if the acid entered the cells only by simple diffusion.application/pdfporTransport of acetic acid in Zygosaccharomyces bailii: effects of ethanol and implications on the resistance of the yeast to acid environmentsPersonalSousa, Maria JoãoDSpacehttp://dspace.org/items/da5c7615-9844-4fdf-b4d4-64d0c8e72de0DSpacehttp://dspace.org/items/da5c7615-9844-4fdf-b4d4-64d0c8e72de0SousaMaria JoãoCiência IDhttps://www.ciencia-id.ptD91E-3571-3979ORCIDhttp://orcid.org0000-0002-9946-4926Scopus Author IDhttps://www.scopus.com55230282700PersonalEstevinho, Leticia M.DSpacehttp://dspace.org/items/4a1d5ba2-1854-4ca5-89a4-73f35e964df9DSpacehttp://dspace.org/items/4a1d5ba2-1854-4ca5-89a4-73f35e964df9EstevinhoLetícia M.Ciência IDhttps://www.ciencia-id.ptBA14-09D6-A406ORCIDhttp://orcid.org0000-0002-9249-1948Scopus Author IDhttps://www.scopus.com6506577664Scopus Author IDhttps://www.scopus.com57211945650Côrte-Real, ManuelaLeão, CecíliaHostingInstitutionOrganizationalBiblioteca Digital da UPBe-mailmailto:dspace@unipb.ptdspace@unipb.ptISSNIsPartOf0099-2240DOIIsPartOf10.1128/aem.62.9.3152-3157.19962011-05-19T09:42:56Z19961996-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/4395http://purl.org/coar/access_right/c_abf2open accessZygosaccharomyces bailii255431 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.unipb.pt/bitstreams/5382b11a-1a90-40d4-ad20-9009f11097fa/downloadApplied and Environmental Microbiology31523157 |
| spellingShingle | Transport of acetic acid in Zygosaccharomyces bailii: effects of ethanol and implications on the resistance of the yeast to acid environments Sousa, Maria João Zygosaccharomyces bailii |
| status | SINGLETON |
| subject.fl_str_mv | Zygosaccharomyces bailii |
| title | Transport of acetic acid in Zygosaccharomyces bailii: effects of ethanol and implications on the resistance of the yeast to acid environments |
| title_full | Transport of acetic acid in Zygosaccharomyces bailii: effects of ethanol and implications on the resistance of the yeast to acid environments |
| title_fullStr | Transport of acetic acid in Zygosaccharomyces bailii: effects of ethanol and implications on the resistance of the yeast to acid environments |
| title_full_unstemmed | Transport of acetic acid in Zygosaccharomyces bailii: effects of ethanol and implications on the resistance of the yeast to acid environments |
| title_short | Transport of acetic acid in Zygosaccharomyces bailii: effects of ethanol and implications on the resistance of the yeast to acid environments |
| title_sort | Transport of acetic acid in Zygosaccharomyces bailii: effects of ethanol and implications on the resistance of the yeast to acid environments |
| topic | Zygosaccharomyces bailii |
| topic_facet | Zygosaccharomyces bailii |
| url | http://hdl.handle.net/10198/4395 |
| visible | 1 |
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