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Combined detection of nitrite and bioelectrical activity using microelectrode arrays and a phosphate buffered saline solution

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Summary:This paper presents the manufacturing of amicroelectrode array, on printed circuit boards (PCB) and onsilicon substrate, composed of 60 to 64 gold microelectrodes(between 4 µm and 70 µm each in diameter). The sensor has ameasurement total area of 0.5 cm in radius, one referenceelectrode (1 mm2 in area) and 28.4 mm wide and 28.4 mm long.These microelectrodes were used for checking and logging ofextracelullar local field potential of cell culture and nitritemeasurement in a phosphate buffered saline solution(electrolytical aqueous medium). In addition, an apparatus toshield from electromagnetic interference for connecting thearrays was designed to allow the capture of electrochemicalreactions or electronic signals by the microelectrodes, forexample: nitrite or cardiac potential measurement, respectively.Finally, biocompatibility tests of the array structures wereperformed. The preliminary electrical and biocompatibilitytesting, along with the collected data, has shown promisingresults pointing to the development of an accurate sensor afterthe completion of this study. The sensor has potentially abroader range of applications with only a few adaptations anddue its good accuracy it can be a very useful resource for manychemical and biological applications.
Main Authors:Almeida, Fernando Luis de
Other Authors:Souza Junior, Wilmar; Moraes, Yann Lima; dos Santos Filho, Sebastião Gomes; Veronez, Douglas; Cestari, Idagene
Subject:extracelullar local field potentials myoblasts gold microelectrodes nitrite heart.
Year:2021
Country:Portugal
Document type:article
Access type:unknown
Associated institution:Instituto Superior de Engenharia de Lisboa
Language:English
Origin:i-ETC : ISEL Academic Journal of Electronics Telecommunications and Computers

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