Publicação
SEMICONDUCTOR DEVICE FOR DETECTION OF FRET SIGNALS
| Resumo: | The transducer consists on a semiconductor device based on two stacked-i-n heterostructures, that were designed to detect the emissions of the fluorescence resonance energy transfer between fluorophores in the cyan (470 nm) and yellow (588 nm) range of the spectrum. This research represents a preliminary study on the use of such wavelength sensitive devices as photodetectors for this kind of application. The device was characterized through optoelectronic measurements concerning spectral response measurements under different electrical and optical biasing conditions. To simulate the FRET pairs a chromatic time dependent combination of cyan and yellow wavelengths was applied to the device. The generated photocurrent was measured under reverse and forward bias to readout the output photocurrent signal. Different wavelength biasing light was also superimposed. Results show that under reverse bias the photocurrent signal presents four separate levels each one assigned to the different wavelength combinations of the FRET pairs. If a blue background is superimposed the yellow channel is enhanced and the cyan suppressed while under red irradiation the opposite behavior occurs. So under suitable biasing light the transducer is able to detect separately the cyan and yellow fluorescence pairs. An electrical model, supported by a numerical simulation supports the transduction mechanism of the device. |
|---|---|
| Autores principais: | Louro, Paula |
| Outros Autores: | Vieira, M.; Vieira, M. A.; Karmali, A.; Fernades, M. |
| Assunto: | Semiconductor Devices; Optoelectronics; Electronics FRET; biosensor; spectral response; pinpin photodiode |
| Ano: | 2013 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | unknown |
| Instituição associada: | Instituto Superior de Engenharia de Lisboa |
| Idioma: | inglês |
| Origem: | i-ETC : ISEL Academic Journal of Electronics Telecommunications and Computers |
| _version_ | 1868798937906806784 |
|---|---|
| author | Louro, Paula |
| author2 | Vieira, M. Vieira, M. A. Karmali, A. Fernades, M. |
| author2_role | author author author author |
| author_facet | Louro, Paula Vieira, M. Vieira, M. A. Karmali, A. Fernades, M. |
| author_role | author |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Louro, Paula\"},{\"Person.name\":\"Vieira, M.\"},{\"Person.name\":\"Vieira, M. A.\"},{\"Person.name\":\"Karmali, A.\"},{\"Person.name\":\"Fernades, M.\"}] |
| datacite.creators.creator.creatorName.fl_str_mv | Louro, Paula Vieira, M. Vieira, M. A. Karmali, A. Fernades, M. |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Semiconductor Devices; Optoelectronics; Electronics FRET; biosensor; spectral response; pinpin photodiode |
| datacite.titles.title.fl_str_mv | SEMICONDUCTOR DEVICE FOR DETECTION OF FRET SIGNALS |
| dc.creator.none.fl_str_mv | Louro, Paula Vieira, M. Vieira, M. A. Karmali, A. Fernades, M. |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://doi.org/10.34629/ipl.isel.i-ETC.4 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | ISEL - High Institute of Engineering of Lisbon |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.source.none.fl_str_mv | i-ETC : ISEL Academic Journal of Electronics Telecommunications and Computers; Vol 2, No 1 (2013): The CETC2011 Issue; ID-2 i-ETC : ISEL Academic Journal of Electronics Telecommunications and Computers; Vol 2, No 1 (2013): The CETC2011 Issue; ID-2 2182-4010 |
| dc.subject.none.fl_str_mv | Semiconductor Devices; Optoelectronics; Electronics FRET; biosensor; spectral response; pinpin photodiode |
| dc.title.fl_str_mv | SEMICONDUCTOR DEVICE FOR DETECTION OF FRET SIGNALS |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 http://purl.org/coar/resource_type/c_1843 |
| description | The transducer consists on a semiconductor device based on two stacked-i-n heterostructures, that were designed to detect the emissions of the fluorescence resonance energy transfer between fluorophores in the cyan (470 nm) and yellow (588 nm) range of the spectrum. This research represents a preliminary study on the use of such wavelength sensitive devices as photodetectors for this kind of application. The device was characterized through optoelectronic measurements concerning spectral response measurements under different electrical and optical biasing conditions. To simulate the FRET pairs a chromatic time dependent combination of cyan and yellow wavelengths was applied to the device. The generated photocurrent was measured under reverse and forward bias to readout the output photocurrent signal. Different wavelength biasing light was also superimposed. Results show that under reverse bias the photocurrent signal presents four separate levels each one assigned to the different wavelength combinations of the FRET pairs. If a blue background is superimposed the yellow channel is enhanced and the cyan suppressed while under red irradiation the opposite behavior occurs. So under suitable biasing light the transducer is able to detect separately the cyan and yellow fluorescence pairs. An electrical model, supported by a numerical simulation supports the transduction mechanism of the device. |
| dirty | 0 |
| eu_rights_str_mv | unknown |
| format | article |
| id | ietc_662101e5d065ed23636867d0c601d82d |
| identifier.doi.fl_str_mv | https://doi.org/10.34629/ipl.isel.i-ETC.4 |
| instacron_str | isel |
| institution | Instituto Superior de Engenharia de Lisboa |
| instname_str | Instituto Superior de Engenharia de Lisboa |
| language | eng |
| network_acronym_str | ietc |
| network_name_str | i-ETC : ISEL Academic Journal of Electronics Telecommunications and Computers |
| oai_identifier_str | oai:i-ETC.journals.isel.pt:article/4 |
| organization_str_mv | urn:organizationAcronym:isel |
| person_str_mv | Louro, Paula Vieira, M. Vieira, M. A. Karmali, A. Fernades, M. |
| publishDate | 2013 |
| publisher.none.fl_str_mv | ISEL - High Institute of Engineering of Lisbon |
| reponame_str | i-ETC : ISEL Academic Journal of Electronics Telecommunications and Computers |
| repository_id_str | urn:repositoryAcronym:ietc |
| service_str_mv | urn:repositoryAcronym:ietc |
| spelling | en-USSEMICONDUCTOR DEVICE FOR DETECTION OF FRET SIGNALSLouro, PaulaVieira, M.Vieira, M. A.Karmali, A.Fernades, M.Semiconductor Devices; Optoelectronics; ElectronicsFRET; biosensor; spectral response; pinpin photodiodeCopyright (c) 2013 i-ETC : ISEL Academic Journal of Electronics Telecommunications and Computershttp://purl.org/coar/access_right/c_abf2http://creativecommons.org/licenses/by-nc/4.0https://doi.org/10.34629/ipl.isel.i-ETC.4DOIoai:i-ETC.journals.isel.pt:article/4OAIhttp://journals.isel.pt/index.php/i-ETC/article/view/4URLhttps://doi.org/10.34629/ipl.isel.i-ETC.4DOIhttp://journals.isel.pt/index.php/i-ETC/article/view/4/4URLHasVersion2013-06-27T00:00:00Zen-USThe transducer consists on a semiconductor device based on two stacked-i-n heterostructures, that were designed to detect the emissions of the fluorescence resonance energy transfer between fluorophores in the cyan (470 nm) and yellow (588 nm) range of the spectrum. This research represents a preliminary study on the use of such wavelength sensitive devices as photodetectors for this kind of application. The device was characterized through optoelectronic measurements concerning spectral response measurements under different electrical and optical biasing conditions. To simulate the FRET pairs a chromatic time dependent combination of cyan and yellow wavelengths was applied to the device. The generated photocurrent was measured under reverse and forward bias to readout the output photocurrent signal. Different wavelength biasing light was also superimposed. Results show that under reverse bias the photocurrent signal presents four separate levels each one assigned to the different wavelength combinations of the FRET pairs. If a blue background is superimposed the yellow channel is enhanced and the cyan suppressed while under red irradiation the opposite behavior occurs. So under suitable biasing light the transducer is able to detect separately the cyan and yellow fluorescence pairs. An electrical model, supported by a numerical simulation supports the transduction mechanism of the device.ISEL - High Institute of Engineering of Lisbonapplication/pdfen-USi-ETC : ISEL Academic Journal of Electronics Telecommunications and Computers; Vol 2, No 1 (2013): The CETC2011 Issue; ID-2pt-PTi-ETC : ISEL Academic Journal of Electronics Telecommunications and Computers; Vol 2, No 1 (2013): The CETC2011 Issue; ID-22182-4010engjournal articlehttp://purl.org/coar/resource_type/c_6501literatureVoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_1843 other research product |
| spellingShingle | SEMICONDUCTOR DEVICE FOR DETECTION OF FRET SIGNALS Louro, Paula Semiconductor Devices; Optoelectronics; Electronics FRET; biosensor; spectral response; pinpin photodiode |
| status | SINGLETON |
| status_str | VoR |
| subject.fl_str_mv | Semiconductor Devices; Optoelectronics; Electronics FRET; biosensor; spectral response; pinpin photodiode |
| title | SEMICONDUCTOR DEVICE FOR DETECTION OF FRET SIGNALS |
| title_full | SEMICONDUCTOR DEVICE FOR DETECTION OF FRET SIGNALS |
| title_fullStr | SEMICONDUCTOR DEVICE FOR DETECTION OF FRET SIGNALS |
| title_full_unstemmed | SEMICONDUCTOR DEVICE FOR DETECTION OF FRET SIGNALS |
| title_short | SEMICONDUCTOR DEVICE FOR DETECTION OF FRET SIGNALS |
| title_sort | SEMICONDUCTOR DEVICE FOR DETECTION OF FRET SIGNALS |
| topic | Semiconductor Devices; Optoelectronics; Electronics FRET; biosensor; spectral response; pinpin photodiode |
| topic_facet | Semiconductor Devices; Optoelectronics; Electronics FRET; biosensor; spectral response; pinpin photodiode |
| url | https://doi.org/10.34629/ipl.isel.i-ETC.4 |
| visible | 1 |
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