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SEMICONDUCTOR DEVICE FOR DETECTION OF FRET SIGNALS

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Bibliographic Details
Summary:The transducer consists on a semiconductor device based on two stacked-i-n  heterostructures, that were designed to detect the emissions of the fluorescence resonance energy transfer between fluorophores in the cyan (470 nm) and yellow (588 nm) range of the spectrum. This research represents a preliminary study on the use of such wavelength sensitive devices as photodetectors for this kind of application. The device was characterized through optoelectronic measurements concerning spectral response measurements under different electrical and optical biasing conditions. To simulate the FRET pairs a chromatic time dependent combination of cyan and yellow wavelengths was applied to the device. The generated photocurrent was measured under reverse and forward bias to readout the output photocurrent signal. Different wavelength biasing light was also superimposed. Results show that under reverse bias the photocurrent signal presents four separate levels each one assigned to the different wavelength combinations of the FRET pairs. If a blue background is superimposed the yellow channel is enhanced and the cyan suppressed while under red irradiation the opposite behavior occurs. So under suitable biasing light the transducer is able to detect separately the cyan and yellow fluorescence pairs. An electrical model, supported by a numerical simulation supports the transduction mechanism of the device.
Main Authors:Louro, Paula
Other Authors:Vieira, M.; Vieira, M. A.; Karmali, A.; Fernades, M.
Subject:Semiconductor Devices; Optoelectronics; Electronics FRET; biosensor; spectral response; pinpin photodiode
Year:2013
Country:Portugal
Document type:article
Access type:unknown
Associated institution:Instituto Superior de Engenharia de Lisboa
Language:English
Origin:i-ETC : ISEL Academic Journal of Electronics Telecommunications and Computers
Description
Summary:The transducer consists on a semiconductor device based on two stacked-i-n  heterostructures, that were designed to detect the emissions of the fluorescence resonance energy transfer between fluorophores in the cyan (470 nm) and yellow (588 nm) range of the spectrum. This research represents a preliminary study on the use of such wavelength sensitive devices as photodetectors for this kind of application. The device was characterized through optoelectronic measurements concerning spectral response measurements under different electrical and optical biasing conditions. To simulate the FRET pairs a chromatic time dependent combination of cyan and yellow wavelengths was applied to the device. The generated photocurrent was measured under reverse and forward bias to readout the output photocurrent signal. Different wavelength biasing light was also superimposed. Results show that under reverse bias the photocurrent signal presents four separate levels each one assigned to the different wavelength combinations of the FRET pairs. If a blue background is superimposed the yellow channel is enhanced and the cyan suppressed while under red irradiation the opposite behavior occurs. So under suitable biasing light the transducer is able to detect separately the cyan and yellow fluorescence pairs. An electrical model, supported by a numerical simulation supports the transduction mechanism of the device.

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