Publication
Multifunctional Gelatin/Chitosan Electrospun Wound Dressing Dopped with Undaria pinnatifida Phlorotannin-Enriched Extract for Skin Regeneration
| Summary: | The similarities of electrospun fibers with the skin extracellular matrix (ECM) make them promising structures for advanced wound dressings. Moreover, infection and resistance in wounds are a major health concern that may be reduced with antibacterial wound dressings. In this work, a multifunctional wound dressing was developed based on gelatin/chitosan hybrid fibers dopped with phlorotannin-enrich extract from the seaweed Undaria pinnatifida. The intrinsic electrospun structure properties combined with the antimicrobial and anti-inflammatory properties of phlorotannin-enrich extract will enhance the wound healing process. Electrospun meshes were produced by incorporating 1 or 2 wt% of extract, and the structure without extract was used as a control. Physico-chemical, mechanical, and biological properties were evaluated for all conditions. Results demonstrated that all developed samples presented a homogenous fiber deposition with the average diameters closer to the native ECM fibrils, and high porosities (~90%) that will be crucial to control the wound moist environment. According to the tensile test assays, the incorporation of phlorotannin-enriched extract enhances the elastic performance of the samples. Additionally, the extract incorporation made the structure stable over time since its in vitro degradation rates decreased under enzymatic medium. Extract release profile demonstrated a longstanding delivery (up to 160 days), reaching a maximum value of ~98% over time. Moreover, the preliminary antimicrobial results confirm the mesh’s antimicrobial activity against Pseudomonas aeruginosa and Staphylococcus aureus. In terms of biological characterization, no condition presented cytotoxicity effects on hDNF cells, allowing their adhesion and proliferation over 14 days, except the condition of 2 wt% after 7 days. Overall, the electrospun structure comprising phlorotannins-enriched extract is a promising bioactive structure with potential to be used as a drug delivery system for skin regeneration by reducing the bacterial infection in the wound bed. |
|---|---|
| Main Authors: | Ferreira, Carolina A. M. |
| Other Authors: | Januário, Adriana P.; Félix, Rafael; Alves, Nuno; Lemos, Marco F.L.; Dias, Juliana R. |
| Subject: | Chronic wounds Chitosan/gelatin/ phlorotannin-enriched extract Electrospun wound dressing Multifunctional structures Wound healing |
| Year: | 2021 |
| Country: | Portugal |
| Document type: | article |
| Access type: | open access |
| Associated institution: | Universidade de Leiria e Oeste |
| Language: | English |
| Origin: | IC-online |
| _version_ | 1877756050104385536 |
|---|---|
| author | Ferreira, Carolina A. M. |
| author2 | Januário, Adriana P. Félix, Rafael Alves, Nuno Lemos, Marco F.L. Dias, Juliana R. |
| author2_role | author author author author author |
| author_facet | Ferreira, Carolina A. M. Januário, Adriana P. Félix, Rafael Alves, Nuno Lemos, Marco F.L. Dias, Juliana R. |
| author_role | author |
| contributor_name_str_mv | Repositório IC-Online |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Ferreira, Carolina A. M.\",\"Person.identifier.cienciaID\":\"3215-5FE5-B516\",\"Person.identifier.orcid\":\"0000-0002-6838-3744\"},{\"Person.name\":\"Januário, Adriana P.\",\"Person.identifier.cienciaID\":\"241B-FEA3-3BED\",\"Person.identifier.orcid\":\"0000-0002-3228-922X\"},{\"Person.name\":\"Félix, Rafael\",\"Person.identifier.cienciaID\":\"A315-5CB0-097C\",\"Person.identifier.orcid\":\"0000-0003-4766-3736\",\"Person.identifier.scopusAuthorID\":\"57200377641\"},{\"Person.name\":\"Alves, Nuno\",\"Person.identifier.cienciaID\":\"311E-1559-8F6C\",\"Person.identifier.orcid\":\"0000-0002-5016-0868\",\"Person.identifier.researcherID\":\"N-4073-2013\",\"Person.identifier.scopusAuthorID\":\"7006403383\"},{\"Person.name\":\"Lemos, Marco F.L.\",\"Person.identifier.cienciaID\":\"971F-ACCA-C0D1\",\"Person.identifier.orcid\":\"0000-0001-9887-1864\",\"Person.identifier.researcherID\":\"F-7951-2011\",\"Person.identifier.scopusAuthorID\":\"7006042884\"},{\"Person.name\":\"Dias, Juliana R.\",\"Person.identifier.cienciaID\":\"0612-D971-F00D\",\"Person.identifier.orcid\":\"0000-0002-7079-1069\",\"Person.identifier.researcherID\":\"A-6086-2014\",\"Person.identifier.researcherID\":\"V-9083-2017\",\"Person.identifier.scopusAuthorID\":\"55749822200\",\"Person.identifier.scopusAuthorID\":\"55493446000\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório IC-Online |
| datacite.creators.creator.creatorName.fl_str_mv | Ferreira, Carolina A. M. Januário, Adriana P. Félix, Rafael Alves, Nuno Lemos, Marco F.L. Dias, Juliana R. |
| datacite.date.Accepted.fl_str_mv | 2021-12-14T00:00:00Z |
| datacite.date.available.fl_str_mv | 2023-04-13T15:29:33Z |
| datacite.date.embargoed.fl_str_mv | 2023-04-13T15:29:33Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Chronic wounds Chitosan/gelatin/ phlorotannin-enriched extract Electrospun wound dressing Multifunctional structures Wound healing |
| datacite.titles.title.fl_str_mv | Multifunctional Gelatin/Chitosan Electrospun Wound Dressing Dopped with Undaria pinnatifida Phlorotannin-Enriched Extract for Skin Regeneration |
| dc.contributor.none.fl_str_mv | Repositório IC-Online |
| dc.creator.none.fl_str_mv | Ferreira, Carolina A. M. Januário, Adriana P. Félix, Rafael Alves, Nuno Lemos, Marco F.L. Dias, Juliana R. |
| dc.date.Accepted.fl_str_mv | 2021-12-14T00:00:00Z |
| dc.date.available.fl_str_mv | 2023-04-13T15:29:33Z |
| dc.date.embargoed.fl_str_mv | 2023-04-13T15:29:33Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.8/8381 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | MDPI |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Chronic wounds Chitosan/gelatin/ phlorotannin-enriched extract Electrospun wound dressing Multifunctional structures Wound healing |
| dc.title.fl_str_mv | Multifunctional Gelatin/Chitosan Electrospun Wound Dressing Dopped with Undaria pinnatifida Phlorotannin-Enriched Extract for Skin Regeneration |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The similarities of electrospun fibers with the skin extracellular matrix (ECM) make them promising structures for advanced wound dressings. Moreover, infection and resistance in wounds are a major health concern that may be reduced with antibacterial wound dressings. In this work, a multifunctional wound dressing was developed based on gelatin/chitosan hybrid fibers dopped with phlorotannin-enrich extract from the seaweed Undaria pinnatifida. The intrinsic electrospun structure properties combined with the antimicrobial and anti-inflammatory properties of phlorotannin-enrich extract will enhance the wound healing process. Electrospun meshes were produced by incorporating 1 or 2 wt% of extract, and the structure without extract was used as a control. Physico-chemical, mechanical, and biological properties were evaluated for all conditions. Results demonstrated that all developed samples presented a homogenous fiber deposition with the average diameters closer to the native ECM fibrils, and high porosities (~90%) that will be crucial to control the wound moist environment. According to the tensile test assays, the incorporation of phlorotannin-enriched extract enhances the elastic performance of the samples. Additionally, the extract incorporation made the structure stable over time since its in vitro degradation rates decreased under enzymatic medium. Extract release profile demonstrated a longstanding delivery (up to 160 days), reaching a maximum value of ~98% over time. Moreover, the preliminary antimicrobial results confirm the mesh’s antimicrobial activity against Pseudomonas aeruginosa and Staphylococcus aureus. In terms of biological characterization, no condition presented cytotoxicity effects on hDNF cells, allowing their adhesion and proliferation over 14 days, except the condition of 2 wt% after 7 days. Overall, the electrospun structure comprising phlorotannins-enriched extract is a promising bioactive structure with potential to be used as a drug delivery system for skin regeneration by reducing the bacterial infection in the wound bed. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10400.8/8381 |
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| institution | Universidade de Leiria e Oeste |
| instname_str | Universidade de Leiria e Oeste |
| language | eng |
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| oai_identifier_str | oai:iconline.ulo.pt:10400.8/8381 |
| organization_str_mv | urn:organizationAcronym:ulo |
| person_str_mv | Ferreira, Carolina A. M. Ferreira, Carolina A. M. https://www.ciencia-id.pt/3215-5FE5-B516 3215-5FE5-B516 http://orcid.org/0000-0002-6838-3744 0000-0002-6838-3744 Januário, Adriana P. Januário, Adriana P. https://www.ciencia-id.pt/241B-FEA3-3BED 241B-FEA3-3BED http://orcid.org/0000-0002-3228-922X 0000-0002-3228-922X Félix, Rafael Félix, Rafael https://www.ciencia-id.pt/A315-5CB0-097C A315-5CB0-097C http://orcid.org/0000-0003-4766-3736 0000-0003-4766-3736 Alves, Nuno Alves, Nuno https://www.ciencia-id.pt/311E-1559-8F6C 311E-1559-8F6C http://orcid.org/0000-0002-5016-0868 0000-0002-5016-0868 Lemos, Marco F.L. Lemos, Marco F.L. https://www.ciencia-id.pt/971F-ACCA-C0D1 971F-ACCA-C0D1 http://orcid.org/0000-0001-9887-1864 0000-0001-9887-1864 Dias, Juliana R. Dias, Juliana R. https://www.ciencia-id.pt/0612-D971-F00D 0612-D971-F00D http://orcid.org/0000-0002-7079-1069 0000-0002-7079-1069 |
| publishDate | 2021 |
| publisher.none.fl_str_mv | MDPI |
| repo_facet_str | urn:repositoryAcronym:iconline{{{_:::_}}}IC-online |
| reponame_str | IC-online |
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| spelling | engMDPIpt_PTThe similarities of electrospun fibers with the skin extracellular matrix (ECM) make them promising structures for advanced wound dressings. Moreover, infection and resistance in wounds are a major health concern that may be reduced with antibacterial wound dressings. In this work, a multifunctional wound dressing was developed based on gelatin/chitosan hybrid fibers dopped with phlorotannin-enrich extract from the seaweed Undaria pinnatifida. The intrinsic electrospun structure properties combined with the antimicrobial and anti-inflammatory properties of phlorotannin-enrich extract will enhance the wound healing process. Electrospun meshes were produced by incorporating 1 or 2 wt% of extract, and the structure without extract was used as a control. Physico-chemical, mechanical, and biological properties were evaluated for all conditions. Results demonstrated that all developed samples presented a homogenous fiber deposition with the average diameters closer to the native ECM fibrils, and high porosities (~90%) that will be crucial to control the wound moist environment. According to the tensile test assays, the incorporation of phlorotannin-enriched extract enhances the elastic performance of the samples. Additionally, the extract incorporation made the structure stable over time since its in vitro degradation rates decreased under enzymatic medium. Extract release profile demonstrated a longstanding delivery (up to 160 days), reaching a maximum value of ~98% over time. Moreover, the preliminary antimicrobial results confirm the mesh’s antimicrobial activity against Pseudomonas aeruginosa and Staphylococcus aureus. In terms of biological characterization, no condition presented cytotoxicity effects on hDNF cells, allowing their adhesion and proliferation over 14 days, except the condition of 2 wt% after 7 days. Overall, the electrospun structure comprising phlorotannins-enriched extract is a promising bioactive structure with potential to be used as a drug delivery system for skin regeneration by reducing the bacterial infection in the wound bed.application/pdfpt_PTMultifunctional Gelatin/Chitosan Electrospun Wound Dressing Dopped with Undaria pinnatifida Phlorotannin-Enriched Extract for Skin RegenerationPersonalFerreira, Carolina A. M.DSpacehttp://dspace.org/items/6424f682-0c7d-4236-95f4-77f6091208e8DSpacehttp://dspace.org/items/6424f682-0c7d-4236-95f4-77f6091208e8Marzia FerreiraCarolina AlexandraCiência IDhttps://www.ciencia-id.pt3215-5FE5-B516ORCIDhttp://orcid.org0000-0002-6838-3744PersonalJanuário, Adriana P.DSpacehttp://dspace.org/items/576c1b04-ad90-4771-80f0-e42f080e3b2bDSpacehttp://dspace.org/items/576c1b04-ad90-4771-80f0-e42f080e3b2bJanuárioAdriana P.Ciência IDhttps://www.ciencia-id.pt241B-FEA3-3BEDORCIDhttp://orcid.org0000-0002-3228-922XPersonalFélix, RafaelDSpacehttp://dspace.org/items/d4d70589-8897-4538-b004-bfe2ce623b69DSpacehttp://dspace.org/items/d4d70589-8897-4538-b004-bfe2ce623b69Faria da Costa FélixRafael AlexandreCiência IDhttps://www.ciencia-id.ptA315-5CB0-097CORCIDhttp://orcid.org0000-0003-4766-3736Scopus Author IDhttps://www.scopus.com57200377641PersonalAlves, NunoDSpacehttp://dspace.org/items/bbd46a74-b77e-4539-a5fe-62ee95cdc4faDSpacehttp://dspace.org/items/bbd46a74-b77e-4539-a5fe-62ee95cdc4faAlvesNunoCiência IDhttps://www.ciencia-id.pt311E-1559-8F6CORCIDhttp://orcid.org0000-0002-5016-0868Researcher IDhttps://www.researcherid.comN-4073-2013Scopus Author IDhttps://www.scopus.com7006403383PersonalLemos, Marco F.L.DSpacehttp://dspace.org/items/f21e5540-df76-43e9-ad64-93edd70da1f1DSpacehttp://dspace.org/items/f21e5540-df76-43e9-ad64-93edd70da1f1LemosMarcoCiência IDhttps://www.ciencia-id.pt971F-ACCA-C0D1ORCIDhttp://orcid.org0000-0001-9887-1864Researcher IDhttps://www.researcherid.comF-7951-2011Scopus Author IDhttps://www.scopus.com7006042884PersonalDias, Juliana R.DSpacehttp://dspace.org/items/9e70a657-1b71-4366-ab59-b9f988a19d5aDSpacehttp://dspace.org/items/9e70a657-1b71-4366-ab59-b9f988a19d5aDiasJulianaCiência IDhttps://www.ciencia-id.pt0612-D971-F00DORCIDhttp://orcid.org0000-0002-7079-1069Researcher IDhttps://www.researcherid.comA-6086-2014Researcher IDhttps://www.researcherid.comV-9083-2017Scopus Author IDhttps://www.scopus.com55749822200Scopus Author IDhttps://www.scopus.com55493446000HostingInstitutionOrganizationalRepositório IC-Onlinee-mailmailto:repositorio@ulo.ptrepositorio@ulo.ptISSNIsPartOf1999-4923DOIIsPartOf10.3390/ pharmaceutics131221522023-04-13T15:29:33Z2021-12-142021-12-14T00:00:00ZHandlehttp://hdl.handle.net/10400.8/8381http://purl.org/coar/access_right/c_abf2open accessChronic woundsChitosan/gelatin/ phlorotannin-enriched extractElectrospun wound dressingMultifunctional structuresWound healing3819851 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2021-12-14http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://iconline.ulo.pt/bitstreams/a000f287-91b2-4827-8b3b-c48b967b7647/downloadPharmaceutics13 |
| spellingShingle | Multifunctional Gelatin/Chitosan Electrospun Wound Dressing Dopped with Undaria pinnatifida Phlorotannin-Enriched Extract for Skin Regeneration Ferreira, Carolina A. M. Chronic wounds Chitosan/gelatin/ phlorotannin-enriched extract Electrospun wound dressing Multifunctional structures Wound healing |
| status | SINGLETON |
| subject.fl_str_mv | Chronic wounds Chitosan/gelatin/ phlorotannin-enriched extract Electrospun wound dressing Multifunctional structures Wound healing |
| title | Multifunctional Gelatin/Chitosan Electrospun Wound Dressing Dopped with Undaria pinnatifida Phlorotannin-Enriched Extract for Skin Regeneration |
| title_full | Multifunctional Gelatin/Chitosan Electrospun Wound Dressing Dopped with Undaria pinnatifida Phlorotannin-Enriched Extract for Skin Regeneration |
| title_fullStr | Multifunctional Gelatin/Chitosan Electrospun Wound Dressing Dopped with Undaria pinnatifida Phlorotannin-Enriched Extract for Skin Regeneration |
| title_full_unstemmed | Multifunctional Gelatin/Chitosan Electrospun Wound Dressing Dopped with Undaria pinnatifida Phlorotannin-Enriched Extract for Skin Regeneration |
| title_short | Multifunctional Gelatin/Chitosan Electrospun Wound Dressing Dopped with Undaria pinnatifida Phlorotannin-Enriched Extract for Skin Regeneration |
| title_sort | Multifunctional Gelatin/Chitosan Electrospun Wound Dressing Dopped with Undaria pinnatifida Phlorotannin-Enriched Extract for Skin Regeneration |
| topic | Chronic wounds Chitosan/gelatin/ phlorotannin-enriched extract Electrospun wound dressing Multifunctional structures Wound healing |
| topic_facet | Chronic wounds Chitosan/gelatin/ phlorotannin-enriched extract Electrospun wound dressing Multifunctional structures Wound healing |
| url | http://hdl.handle.net/10400.8/8381 |
| visible | 1 |
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