Na microrregião de Viçosa-MG, foi testado um controle estratégico experimental do carrapato de bovinos Boophilus microplus em bezerros 3/4 Holandês x Zebu pela administração do Amitraz, de outubro de 1990 a fevereiro de 1991, em cinco banhos intervalados de 28 dias. O controle estratégico apresentou efeito redutor sobre o número de formas adultas de Boophilus microplus nos animais tratados, mas não foi suficiente para impedir que estes níveis se elevassem após o início de condições meteorológicas satisfatórias para o desenvolvimento de carrapato na primavera de 1991.

Bovines of three and four month of old age received monthly and subcutaneously, three immunizations of synthetic peptide SBm7462 emulsified in saponin. The development both cellular and humoral immune response were evaluated. It s was achieved identifying the IgG1 and IgG2 antigen specific isotypes, determining of the profile of circulating lymphocytes (CD4+, CD8+, CD21+ and WC1+) by flow citometry and following of the microscopic events in lymphonodes using histology and immunochemistry techniques. Serologic analysis showed stimulation of the production of antigen-specific immunoglobulin by SBm7462, with predominance of the IgG1 isotype over IgG2 isotype (p<0.05). The histology analysis revealed the activation of the response immune beginning at the seventh day after the first immunization and reactivity evident of germinal centers fifteen days after first immunization. Medullars cords hyperplasia was more evident five days after the second immunization. Clusters of apoptotics cells were detected in entire of the lymphonodes of immunized animals and were more abundant that in control animals. SBm7462antigen was detected in SBm7462 positive cells during whole experiment, and simultaneous to the histology alterations in lymphonodes. Evaluating phenotypic composition of the of peripheral blood lymphocytes, a progressive increase of CD21+ lymphocytes was detected. The variation in total number of T lymphocytes were not statistically significant, although an increment of TCD4+ and WC1+ lymphocytes was observed five days after second immunization. It s suggested that the SBm7462 synthetic peptide induces an antigen-specific immune response which involves mechanisms of the immune system cellular and humoral.

Submitted by Biblioteca Suporte PUCRS (biblioteca.suporte@pucrs.br) on 2022-09-28T15:08:38Z No. of bitstreams: 2 Isolamento_e_seqenciamento_de_fragmentos_de_uma_bilbioteca_de_cDNA_de_glndula_salivar_de_Boophilus_microplus.pdf: 13177 bytes, checksum: d2e17e7dbee6004d200bb57597b5a60e (MD5) Isolamento_e_seqenciamento_de_fragmentos_de_uma_bilbioteca_de_cDNA_de_glndula_salivar_de_Boophilus_microplus.pdf: 13177 bytes, checksum: d2e17e7dbee6004d200bb57597b5a60e (MD5)

Submitted by Biblioteca Suporte PUCRS (biblioteca.suporte@pucrs.br) on 2022-09-28T15:08:47Z No. of bitstreams: 2 Coimunizao_de_bovino_com_calreticulina_e_paramiosina_recombinantes_do_carrapato_Boophilus_microplus.pdf: 14101 bytes, checksum: e7a7aa86885369178b55215d39e5ed76 (MD5) Coimunizao_de_bovino_com_calreticulina_e_paramiosina_recombinantes_do_carrapato_Boophilus_microplus.pdf: 14101 bytes, checksum: e7a7aa86885369178b55215d39e5ed76 (MD5)